Spontaneous activation of spermatozoa motility by routine freeze‐thawing in different fish species
Identifieur interne : 000810 ( Main/Merge ); précédent : 000809; suivant : 000811Spontaneous activation of spermatozoa motility by routine freeze‐thawing in different fish species
Auteurs : B. Dzyuba [République tchèque, Ukraine] ; S. Boryshpolets [République tchèque] ; M. Rodina [République tchèque] ; D. Gela [République tchèque] ; O. Linhart [République tchèque]Source :
- Journal of Applied Ichthyology [ 0175-8659 ] ; 2010-10.
Abstract
In the present study, we investigate the possibility of spermatozoa motility activation resulted from freeze‐thawing, defined by us as spontaneous sperm activation (SSA) in common carp (Cyprinus carpio L.), Eurasian perch (Perca fluviatilis L.), rainbow trout (Oncorhynchus mykiss), Northern pike (Esox lucius L.) and sterlet (Acipenser ruthenus L.) using cryopreservation methods described previously. To evaluate that, the motility percentage and velocity of frozen‐thawed spermatozoa before and after dilution with activating media were used. The SSA phenomenon was found in carp and perch, but not in pike, rainbow trout and sterlet. The motility and velocity of spermatozoa for carp SSA were characterized by 16% (median value) and velocity 98 ± 2 μm s−1 (mean ± 0.95 CI), for perch 5% and 53 ± 5 μm s−1, correspondingly. Significant decrease of sperm velocity was observed, when activation media were applied for thawed sperm in carp, perch, trout and pike, while for sterlet this parameter became significantly increased. We believe that the phenomena of spontaneous activation by freeze‐thawing processes are species‐specific ones.
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DOI: 10.1111/j.1439-0426.2010.01553.x
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To evaluate that, the motility percentage and velocity of frozen‐thawed spermatozoa before and after dilution with activating media were used. The SSA phenomenon was found in carp and perch, but not in pike, rainbow trout and sterlet. The motility and velocity of spermatozoa for carp SSA were characterized by 16% (median value) and velocity 98 ± 2 μm s−1 (mean ± 0.95 CI), for perch 5% and 53 ± 5 μm s−1, correspondingly. Significant decrease of sperm velocity was observed, when activation media were applied for thawed sperm in carp, perch, trout and pike, while for sterlet this parameter became significantly increased. We believe that the phenomena of spontaneous activation by freeze‐thawing processes are species‐specific ones.</div></front></TEI></record>Pour manipuler ce document sous Unix (Dilib)
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