Spontaneous activation of spermatozoa motility by routine freeze‐thawing in different fish species
Identifieur interne : 000831 ( Istex/Corpus ); précédent : 000830; suivant : 000832Spontaneous activation of spermatozoa motility by routine freeze‐thawing in different fish species
Auteurs : B. Dzyuba ; S. Boryshpolets ; M. Rodina ; D. Gela ; O. LinhartSource :
- Journal of Applied Ichthyology [ 0175-8659 ] ; 2010-10.
Abstract
In the present study, we investigate the possibility of spermatozoa motility activation resulted from freeze‐thawing, defined by us as spontaneous sperm activation (SSA) in common carp (Cyprinus carpio L.), Eurasian perch (Perca fluviatilis L.), rainbow trout (Oncorhynchus mykiss), Northern pike (Esox lucius L.) and sterlet (Acipenser ruthenus L.) using cryopreservation methods described previously. To evaluate that, the motility percentage and velocity of frozen‐thawed spermatozoa before and after dilution with activating media were used. The SSA phenomenon was found in carp and perch, but not in pike, rainbow trout and sterlet. The motility and velocity of spermatozoa for carp SSA were characterized by 16% (median value) and velocity 98 ± 2 μm s−1 (mean ± 0.95 CI), for perch 5% and 53 ± 5 μm s−1, correspondingly. Significant decrease of sperm velocity was observed, when activation media were applied for thawed sperm in carp, perch, trout and pike, while for sterlet this parameter became significantly increased. We believe that the phenomena of spontaneous activation by freeze‐thawing processes are species‐specific ones.
Url:
DOI: 10.1111/j.1439-0426.2010.01553.x
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Boryshpolets</name><affiliation><mods:affiliation>University of South Bohemia, Faculty of Fisheries and Protection of Water, South Bohemian Research Center of Aquaculture and Biodiversity of Hydrocenoses, Vodnany, Czech Republic</mods:affiliation></affiliation></author><author><name sortKey="Rodina, M" sort="Rodina, M" uniqKey="Rodina M" first="M." last="Rodina">M. Rodina</name><affiliation><mods:affiliation>University of South Bohemia, Faculty of Fisheries and Protection of Water, South Bohemian Research Center of Aquaculture and Biodiversity of Hydrocenoses, Vodnany, Czech Republic</mods:affiliation></affiliation></author><author><name sortKey="Gela, D" sort="Gela, D" uniqKey="Gela D" first="D." last="Gela">D. 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Dzyuba</name><affiliation><mods:affiliation>University of South Bohemia, Faculty of Fisheries and Protection of Water, South Bohemian Research Center of Aquaculture and Biodiversity of Hydrocenoses, Vodnany, Czech Republic</mods:affiliation></affiliation><affiliation><mods:affiliation>Institute for Problems of Cryobiology & Cryomedicine of National Academy of Sciences of the Ukraine, Kharkov, Ukraine</mods:affiliation></affiliation></author><author><name sortKey="Boryshpolets, S" sort="Boryshpolets, S" uniqKey="Boryshpolets S" first="S." last="Boryshpolets">S. Boryshpolets</name><affiliation><mods:affiliation>University of South Bohemia, Faculty of Fisheries and Protection of Water, South Bohemian Research Center of Aquaculture and Biodiversity of Hydrocenoses, Vodnany, Czech Republic</mods:affiliation></affiliation></author><author><name sortKey="Rodina, M" sort="Rodina, M" uniqKey="Rodina M" first="M." last="Rodina">M. Rodina</name><affiliation><mods:affiliation>University of South Bohemia, Faculty of Fisheries and Protection of Water, South Bohemian Research Center of Aquaculture and Biodiversity of Hydrocenoses, Vodnany, Czech Republic</mods:affiliation></affiliation></author><author><name sortKey="Gela, D" sort="Gela, D" uniqKey="Gela D" first="D." last="Gela">D. Gela</name><affiliation><mods:affiliation>University of South Bohemia, Faculty of Fisheries and Protection of Water, South Bohemian Research Center of Aquaculture and Biodiversity of Hydrocenoses, Vodnany, Czech Republic</mods:affiliation></affiliation></author><author><name sortKey="Linhart, O" sort="Linhart, O" uniqKey="Linhart O" first="O." last="Linhart">O. Linhart</name><affiliation><mods:affiliation>University of South Bohemia, Faculty of Fisheries and Protection of Water, South Bohemian Research Center of Aquaculture and Biodiversity of Hydrocenoses, Vodnany, Czech Republic</mods:affiliation></affiliation></author></analytic><monogr></monogr><series><title level="j">Journal of Applied Ichthyology</title><idno type="ISSN">0175-8659</idno><idno type="eISSN">1439-0426</idno><imprint><publisher>Blackwell Publishing Ltd</publisher><pubPlace>Oxford, UK</pubPlace><date type="published" when="2010-10">2010-10</date><biblScope unit="volume">26</biblScope><biblScope unit="issue">5</biblScope><biblScope unit="page" from="720">720</biblScope><biblScope unit="page" to="725">725</biblScope></imprint><idno type="ISSN">0175-8659</idno></series><idno type="istex">F586C7226DD75370454621E1719E87DB753C1A42</idno><idno type="DOI">10.1111/j.1439-0426.2010.01553.x</idno><idno type="ArticleID">JAI1553</idno></biblStruct></sourceDesc><seriesStmt><idno type="ISSN">0175-8659</idno></seriesStmt></fileDesc><profileDesc><textClass></textClass><langUsage><language ident="en">en</language></langUsage></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">In the present study, we investigate the possibility of spermatozoa motility activation resulted from freeze‐thawing, defined by us as spontaneous sperm activation (SSA) in common carp (Cyprinus carpio L.), Eurasian perch (Perca fluviatilis L.), rainbow trout (Oncorhynchus mykiss), Northern pike (Esox lucius L.) and sterlet (Acipenser ruthenus L.) using cryopreservation methods described previously. To evaluate that, the motility percentage and velocity of frozen‐thawed spermatozoa before and after dilution with activating media were used. The SSA phenomenon was found in carp and perch, but not in pike, rainbow trout and sterlet. The motility and velocity of spermatozoa for carp SSA were characterized by 16% (median value) and velocity 98 ± 2 μm s−1 (mean ± 0.95 CI), for perch 5% and 53 ± 5 μm s−1, correspondingly. Significant decrease of sperm velocity was observed, when activation media were applied for thawed sperm in carp, perch, trout and pike, while for sterlet this parameter became significantly increased. We believe that the phenomena of spontaneous activation by freeze‐thawing processes are species‐specific ones.</div></front></TEI><istex><corpusName>wiley</corpusName><author><json:item><name>B. Dzyuba</name><affiliations><json:string>University of South Bohemia, Faculty of Fisheries and Protection of Water, South Bohemian Research Center of Aquaculture and Biodiversity of Hydrocenoses, Vodnany, Czech Republic</json:string><json:string>Institute for Problems of Cryobiology & Cryomedicine of National Academy of Sciences of the Ukraine, Kharkov, Ukraine</json:string></affiliations></json:item><json:item><name>S. Boryshpolets</name><affiliations><json:string>University of South Bohemia, Faculty of Fisheries and Protection of Water, South Bohemian Research Center of Aquaculture and Biodiversity of Hydrocenoses, Vodnany, Czech Republic</json:string></affiliations></json:item><json:item><name>M. Rodina</name><affiliations><json:string>University of South Bohemia, Faculty of Fisheries and Protection of Water, South Bohemian Research Center of Aquaculture and Biodiversity of Hydrocenoses, Vodnany, Czech Republic</json:string></affiliations></json:item><json:item><name>D. 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The SSA phenomenon was found in carp and perch, but not in pike, rainbow trout and sterlet. The motility and velocity of spermatozoa for carp SSA were characterized by 16% (median value) and velocity 98 ± 2 μm s−1 (mean ± 0.95 CI), for perch 5% and 53 ± 5 μm s−1, correspondingly. Significant decrease of sperm velocity was observed, when activation media were applied for thawed sperm in carp, perch, trout and pike, while for sterlet this parameter became significantly increased. 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type="first">O.</forename><surname>Linhart</surname></persName><affiliation>University of South Bohemia, Faculty of Fisheries and Protection of Water, South Bohemian Research Center of Aquaculture and Biodiversity of Hydrocenoses, Vodnany, Czech Republic</affiliation></author></analytic><monogr><title level="j">Journal of Applied Ichthyology</title><idno type="pISSN">0175-8659</idno><idno type="eISSN">1439-0426</idno><idno type="DOI">10.1111/(ISSN)1439-0426</idno><imprint><publisher>Blackwell Publishing Ltd</publisher><pubPlace>Oxford, UK</pubPlace><date type="published" when="2010-10"></date><biblScope unit="volume">26</biblScope><biblScope unit="issue">5</biblScope><biblScope unit="page" from="720">720</biblScope><biblScope unit="page" to="725">725</biblScope></imprint></monogr><idno type="istex">F586C7226DD75370454621E1719E87DB753C1A42</idno><idno type="DOI">10.1111/j.1439-0426.2010.01553.x</idno><idno type="ArticleID">JAI1553</idno></biblStruct></sourceDesc></fileDesc><profileDesc><creation><date>2010</date></creation><langUsage><language ident="en">en</language></langUsage><abstract xml:lang="en"><p>In the present study, we investigate the possibility of spermatozoa motility activation resulted from freeze‐thawing, defined by us as spontaneous sperm activation (SSA) in common carp (Cyprinus carpio L.), Eurasian perch (Perca fluviatilis L.), rainbow trout (Oncorhynchus mykiss), Northern pike (Esox lucius L.) and sterlet (Acipenser ruthenus L.) using cryopreservation methods described previously. To evaluate that, the motility percentage and velocity of frozen‐thawed spermatozoa before and after dilution with activating media were used. The SSA phenomenon was found in carp and perch, but not in pike, rainbow trout and sterlet. The motility and velocity of spermatozoa for carp SSA were characterized by 16% (median value) and velocity 98 ± 2 μm s−1 (mean ± 0.95 CI), for perch 5% and 53 ± 5 μm s−1, correspondingly. Significant decrease of sperm velocity was observed, when activation media were applied for thawed sperm in carp, perch, trout and pike, while for sterlet this parameter became significantly increased. We believe that the phenomena of spontaneous activation by freeze‐thawing processes are species‐specific ones.</p></abstract></profileDesc><revisionDesc><change when="2010-10">Published</change></revisionDesc></teiHeader></istex:fulltextTEI><json:item><extension>txt</extension><original>false</original><mimetype>text/plain</mimetype><uri>https://api.istex.fr/document/F586C7226DD75370454621E1719E87DB753C1A42/fulltext/txt</uri></json:item></fulltext><metadata><istex:metadataXml wicri:clean="Wiley, elements deleted: body"><istex:xmlDeclaration>version="1.0" encoding="UTF-8" standalone="yes"</istex:xmlDeclaration><istex:document><component version="2.0" type="serialArticle" xml:lang="en"><header><publicationMeta level="product"><publisherInfo><publisherName>Blackwell Publishing Ltd</publisherName><publisherLoc>Oxford, UK</publisherLoc></publisherInfo><doi origin="wiley" registered="yes">10.1111/(ISSN)1439-0426</doi><issn type="print">0175-8659</issn><issn type="electronic">1439-0426</issn><idGroup><id type="product" value="JAI"></id><id type="publisherDivision" value="ST"></id></idGroup><titleGroup><title type="main" sort="JOURNAL OF APPLIED ICHTHYOLOGY">Journal of Applied Ichthyology</title></titleGroup></publicationMeta><publicationMeta level="part" position="10105"><doi origin="wiley">10.1111/jai.2010.26.issue-5</doi><titleGroup><title type="specialIssueTitle">Proceedings of the Second International Workshop on Biology of Fish Gametes
Special Issue Editors: Harald Rosenthal, Juan F. Asturiano, Otomar Linhart, Akos Horvath</title></titleGroup><numberingGroup><numbering type="journalVolume" number="26">26</numbering><numbering type="journalIssue">5</numbering></numberingGroup><coverDate startDate="2010-10">October 2010</coverDate></publicationMeta><publicationMeta level="unit" type="article" position="17" status="forIssue"><doi origin="wiley">10.1111/j.1439-0426.2010.01553.x</doi><idGroup><id type="unit" value="JAI1553"></id></idGroup><countGroup><count type="pageTotal" number="6"></count></countGroup><titleGroup><title type="tocHeading1">Original articles</title></titleGroup><copyright>© 2010 Blackwell Verlag, Berlin</copyright><eventGroup><event type="xmlConverted" agent="Converter:BPG_TO_WML3G version:2.3.19 mode:FullText" date="2010-09-17"></event><event type="firstOnline" date="2010-09-17"></event><event type="publishedOnlineFinalForm" date="2010-09-17"></event><event type="xmlConverted" agent="Converter:WILEY_ML3G_TO_WILEY_ML3GV2 version:3.8.8" date="2014-01-28"></event><event type="xmlConverted" agent="Converter:WML3G_To_WML3G version:4.1.7 mode:FullText,remove_FC" date="2014-10-23"></event></eventGroup><numberingGroup><numbering type="pageFirst" number="720">720</numbering><numbering type="pageLast" number="725">725</numbering></numberingGroup><correspondenceTo><b>Author’s address:</b> B. Dzyuba, University of South Bohemia, Faculty of Fisheries and Protection of Water, Research Institute of Fish Culture and Hydrobiology, Vodnany 389 25, Czech Republic.
E‐mail: <email>bdzuba@yahoo.com</email></correspondenceTo><linkGroup><link type="toTypesetVersion" href="file:JAI.JAI1553.pdf"></link></linkGroup></publicationMeta><contentMeta><unparsedEditorialHistory>Received: March 14, 2010 Accepted: August 3, 2010</unparsedEditorialHistory><countGroup><count type="figureTotal" number="5"></count><count type="tableTotal" number="1"></count></countGroup><titleGroup><title type="main">Spontaneous activation of spermatozoa motility by routine freeze‐thawing in different fish species</title><title type="shortAuthors">B. Dzyuba et al.</title><title type="short">Spontaneous activation of spermatozoa motility</title></titleGroup><creators><creator creatorRole="author" xml:id="cr1" affiliationRef="#a1 #a2"><personName><givenNames>B.</givenNames><familyName>Dzyuba</familyName></personName></creator><creator creatorRole="author" xml:id="cr2" affiliationRef="#a1"><personName><givenNames>S.</givenNames><familyName>Boryshpolets</familyName></personName></creator><creator creatorRole="author" xml:id="cr3" affiliationRef="#a1"><personName><givenNames>M.</givenNames><familyName>Rodina</familyName></personName></creator><creator creatorRole="author" xml:id="cr4" affiliationRef="#a1"><personName><givenNames>D.</givenNames><familyName>Gela</familyName></personName></creator><creator creatorRole="author" xml:id="cr5" affiliationRef="#a1"><personName><givenNames>O.</givenNames><familyName>Linhart</familyName></personName></creator></creators><affiliationGroup><affiliation xml:id="a1" countryCode="CZ"><unparsedAffiliation>University of South Bohemia, Faculty of Fisheries and Protection of Water, South Bohemian Research Center of Aquaculture and Biodiversity of Hydrocenoses, Vodnany, Czech Republic</unparsedAffiliation></affiliation><affiliation xml:id="a2" countryCode="UA"><unparsedAffiliation>Institute for Problems of Cryobiology & Cryomedicine of National Academy of Sciences of the Ukraine, Kharkov, Ukraine</unparsedAffiliation></affiliation></affiliationGroup><abstractGroup><abstract type="main" xml:lang="en"><title type="main">Summary</title><p>In the present study, we investigate the possibility of spermatozoa motility activation resulted from freeze‐thawing, defined by us as spontaneous sperm activation (SSA) in common carp (<i>Cyprinus carpio</i> L.), Eurasian perch (<i>Perca fluviatilis</i> L.), rainbow trout (<i>Oncorhynchus mykiss)</i>, Northern pike (<i>Esox lucius</i> L.) and sterlet (<i>Acipenser ruthenus</i> L.) using cryopreservation methods described previously. To evaluate that, the motility percentage and velocity of frozen‐thawed spermatozoa before and after dilution with activating media were used. The SSA phenomenon was found in carp and perch, but not in pike, rainbow trout and sterlet. The motility and velocity of spermatozoa for carp SSA were characterized by 16% (median value) and velocity 98 ± 2 μm s<sup>−1</sup> (mean ± 0.95 CI), for perch 5% and 53 ± 5 μm s<sup>−1</sup>, correspondingly. Significant decrease of sperm velocity was observed, when activation media were applied for thawed sperm in carp, perch, trout and pike, while for sterlet this parameter became significantly increased. We believe that the phenomena of spontaneous activation by freeze‐thawing processes are species‐specific ones.</p></abstract></abstractGroup></contentMeta></header></component></istex:document></istex:metadataXml><mods version="3.6"><titleInfo lang="en"><title>Spontaneous activation of spermatozoa motility by routine freeze‐thawing in different fish species</title></titleInfo><titleInfo type="abbreviated" lang="en"><title>Spontaneous activation of spermatozoa motility</title></titleInfo><titleInfo type="alternative" contentType="CDATA" lang="en"><title>Spontaneous activation of spermatozoa motility by routine freeze‐thawing in different fish species</title></titleInfo><name type="personal"><namePart type="given">B.</namePart><namePart type="family">Dzyuba</namePart><affiliation>University of South Bohemia, Faculty of Fisheries and Protection of Water, South Bohemian Research Center of Aquaculture and Biodiversity of Hydrocenoses, Vodnany, Czech Republic</affiliation><affiliation>Institute for Problems of Cryobiology & Cryomedicine of National Academy of Sciences of the Ukraine, Kharkov, Ukraine</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">S.</namePart><namePart type="family">Boryshpolets</namePart><affiliation>University of South Bohemia, Faculty of Fisheries and Protection of Water, South Bohemian Research Center of Aquaculture and Biodiversity of Hydrocenoses, Vodnany, Czech Republic</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">M.</namePart><namePart type="family">Rodina</namePart><affiliation>University of South Bohemia, Faculty of Fisheries and Protection of Water, South Bohemian Research Center of Aquaculture and Biodiversity of Hydrocenoses, Vodnany, Czech Republic</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">D.</namePart><namePart type="family">Gela</namePart><affiliation>University of South Bohemia, Faculty of Fisheries and Protection of Water, South Bohemian Research Center of Aquaculture and Biodiversity of Hydrocenoses, Vodnany, Czech Republic</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">O.</namePart><namePart type="family">Linhart</namePart><affiliation>University of South Bohemia, Faculty of Fisheries and Protection of Water, South Bohemian Research Center of Aquaculture and Biodiversity of Hydrocenoses, Vodnany, Czech Republic</affiliation><role><roleTerm type="text">author</roleTerm></role></name><typeOfResource>text</typeOfResource><genre type="article" displayLabel="article"></genre><originInfo><publisher>Blackwell Publishing Ltd</publisher><place><placeTerm type="text">Oxford, UK</placeTerm></place><dateIssued encoding="w3cdtf">2010-10</dateIssued><edition>Received: March 14, 2010 Accepted: August 3, 2010</edition><copyrightDate encoding="w3cdtf">2010</copyrightDate></originInfo><language><languageTerm type="code" authority="rfc3066">en</languageTerm><languageTerm type="code" authority="iso639-2b">eng</languageTerm></language><physicalDescription><internetMediaType>text/html</internetMediaType><extent unit="figures">5</extent><extent unit="tables">1</extent></physicalDescription><abstract lang="en">In the present study, we investigate the possibility of spermatozoa motility activation resulted from freeze‐thawing, defined by us as spontaneous sperm activation (SSA) in common carp (Cyprinus carpio L.), Eurasian perch (Perca fluviatilis L.), rainbow trout (Oncorhynchus mykiss), Northern pike (Esox lucius L.) and sterlet (Acipenser ruthenus L.) using cryopreservation methods described previously. To evaluate that, the motility percentage and velocity of frozen‐thawed spermatozoa before and after dilution with activating media were used. The SSA phenomenon was found in carp and perch, but not in pike, rainbow trout and sterlet. The motility and velocity of spermatozoa for carp SSA were characterized by 16% (median value) and velocity 98 ± 2 μm s−1 (mean ± 0.95 CI), for perch 5% and 53 ± 5 μm s−1, correspondingly. Significant decrease of sperm velocity was observed, when activation media were applied for thawed sperm in carp, perch, trout and pike, while for sterlet this parameter became significantly increased. We believe that the phenomena of spontaneous activation by freeze‐thawing processes are species‐specific ones.</abstract><relatedItem type="host"><titleInfo><title>Journal of Applied Ichthyology</title></titleInfo><genre type="journal">journal</genre><identifier type="ISSN">0175-8659</identifier><identifier type="eISSN">1439-0426</identifier><identifier type="DOI">10.1111/(ISSN)1439-0426</identifier><identifier type="PublisherID">JAI</identifier><part><date>2010</date><detail type="title"><title>Proceedings of the Second International Workshop on Biology of Fish Gametes Special Issue Editors: Harald Rosenthal, Juan F. Asturiano, Otomar Linhart, Akos Horvath</title></detail><detail type="volume"><caption>vol.</caption><number>26</number></detail><detail type="issue"><caption>no.</caption><number>5</number></detail><extent unit="pages"><start>720</start><end>725</end><total>6</total></extent></part></relatedItem><identifier type="istex">F586C7226DD75370454621E1719E87DB753C1A42</identifier><identifier type="DOI">10.1111/j.1439-0426.2010.01553.x</identifier><identifier type="ArticleID">JAI1553</identifier><accessCondition type="use and reproduction" contentType="copyright">© 2010 Blackwell Verlag, Berlin</accessCondition><recordInfo><recordContentSource>WILEY</recordContentSource><recordOrigin>Blackwell Publishing Ltd</recordOrigin></recordInfo></mods></metadata><serie></serie></istex></record>Pour manipuler ce document sous Unix (Dilib)
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