Cryopreservation of sterlet (Acipenser ruthenus) spermatozoa using different cryoprotectants
Identifieur interne : 000718 ( Main/Merge ); précédent : 000717; suivant : 000719Cryopreservation of sterlet (Acipenser ruthenus) spermatozoa using different cryoprotectants
Auteurs : S. Boryshpolets [République tchèque] ; B. Dzyuba [République tchèque, Ukraine] ; M. Rodina [République tchèque] ; S. M. H. Alavi [République tchèque] ; D. Gela [République tchèque] ; O. Linhart [République tchèque]Source :
- Journal of Applied Ichthyology [ 0175-8659 ] ; 2011-10.
Abstract
The present study examined the effectiveness of different cryoprotectan uses for cryopreservation of sterlet (Acipenser ruthenus) sperm. Four different cryoprotectans [dimethyl‐sulfoxide (DMSO), dimethylacetamide (DMA), ethyleneglycol (EG) and methanol (MET)] in concentrations of 5 and 10% in the extender media (30 mm sucrose, 1 mm KCl, 25 mm Tris–HCl pH 8.5) were used for the cryopreservation of sperm from five sterlet males. Percentages of motility, velocity, fertilization and hatching rate were measured. The highest post‐thawing motility was observed in sperm samples cryopreserved with 10% MET (46 ± 19%), 10% DMA (47 ± 18%) and 10% DMSO (45 ± 7%). Fertilization rate in the control (fresh sperm) was 69 ± 9% and the hatching rate 61 ± 8%. A higher hatching rate after cryopreservation was obtained with 10% MET (32 ± 17%) and 5% DMA (23 ± 3%) when compared to data obtained with 10% DMA (9 ± 5%), 5% MET and 5 and 10% DMSO, wherein the hatching rate was around zero. Our results demonstrate that DMA can be used for cryopreservation of sturgeon spermatozoa.
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DOI: 10.1111/j.1439-0426.2011.01866.x
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Dzyuba</name><affiliation wicri:level="1"><country xml:lang="fr">République tchèque</country><wicri:regionArea>Faculty of Fisheries and Protection of Water, Research Institute of Fish Culture and Hydrobiology, University of South Bohemia, Vodnany</wicri:regionArea><wicri:noRegion>Vodnany</wicri:noRegion></affiliation><affiliation wicri:level="1"><country xml:lang="fr">Ukraine</country><wicri:regionArea>Institute for Problems of Cryobiology and Cryomedicine, National Academy of Sciences of the Ukraine, Kharkov</wicri:regionArea><wicri:noRegion>Kharkov</wicri:noRegion></affiliation></author><author><name sortKey="Rodina, M" sort="Rodina, M" uniqKey="Rodina M" first="M." last="Rodina">M. Rodina</name><affiliation wicri:level="1"><country xml:lang="fr">République tchèque</country><wicri:regionArea>Faculty of Fisheries and Protection of Water, Research Institute of Fish Culture and Hydrobiology, University of South Bohemia, Vodnany</wicri:regionArea><wicri:noRegion>Vodnany</wicri:noRegion></affiliation></author><author><name sortKey="Alavi, S M H" sort="Alavi, S M H" uniqKey="Alavi S" first="S. M. H." last="Alavi">S. M. H. Alavi</name><affiliation wicri:level="1"><country xml:lang="fr">République tchèque</country><wicri:regionArea>Faculty of Fisheries and Protection of Water, Research Institute of Fish Culture and Hydrobiology, University of South Bohemia, Vodnany</wicri:regionArea><wicri:noRegion>Vodnany</wicri:noRegion></affiliation></author><author><name sortKey="Gela, D" sort="Gela, D" uniqKey="Gela D" first="D." last="Gela">D. 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Four different cryoprotectans [dimethyl‐sulfoxide (DMSO), dimethylacetamide (DMA), ethyleneglycol (EG) and methanol (MET)] in concentrations of 5 and 10% in the extender media (30 mm sucrose, 1 mm KCl, 25 mm Tris–HCl pH 8.5) were used for the cryopreservation of sperm from five sterlet males. Percentages of motility, velocity, fertilization and hatching rate were measured. The highest post‐thawing motility was observed in sperm samples cryopreserved with 10% MET (46 ± 19%), 10% DMA (47 ± 18%) and 10% DMSO (45 ± 7%). Fertilization rate in the control (fresh sperm) was 69 ± 9% and the hatching rate 61 ± 8%. A higher hatching rate after cryopreservation was obtained with 10% MET (32 ± 17%) and 5% DMA (23 ± 3%) when compared to data obtained with 10% DMA (9 ± 5%), 5% MET and 5 and 10% DMSO, wherein the hatching rate was around zero. Our results demonstrate that DMA can be used for cryopreservation of sturgeon spermatozoa.</div></front></TEI></record>Pour manipuler ce document sous Unix (Dilib)
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