Cryopreservation of sterlet (Acipenser ruthenus) spermatozoa using different cryoprotectants
Identifieur interne : 000300 ( Istex/Corpus ); précédent : 000299; suivant : 000301Cryopreservation of sterlet (Acipenser ruthenus) spermatozoa using different cryoprotectants
Auteurs : S. Boryshpolets ; B. Dzyuba ; M. Rodina ; S. M. H. Alavi ; D. Gela ; O. LinhartSource :
- Journal of Applied Ichthyology [ 0175-8659 ] ; 2011-10.
Abstract
The present study examined the effectiveness of different cryoprotectan uses for cryopreservation of sterlet (Acipenser ruthenus) sperm. Four different cryoprotectans [dimethyl‐sulfoxide (DMSO), dimethylacetamide (DMA), ethyleneglycol (EG) and methanol (MET)] in concentrations of 5 and 10% in the extender media (30 mm sucrose, 1 mm KCl, 25 mm Tris–HCl pH 8.5) were used for the cryopreservation of sperm from five sterlet males. Percentages of motility, velocity, fertilization and hatching rate were measured. The highest post‐thawing motility was observed in sperm samples cryopreserved with 10% MET (46 ± 19%), 10% DMA (47 ± 18%) and 10% DMSO (45 ± 7%). Fertilization rate in the control (fresh sperm) was 69 ± 9% and the hatching rate 61 ± 8%. A higher hatching rate after cryopreservation was obtained with 10% MET (32 ± 17%) and 5% DMA (23 ± 3%) when compared to data obtained with 10% DMA (9 ± 5%), 5% MET and 5 and 10% DMSO, wherein the hatching rate was around zero. Our results demonstrate that DMA can be used for cryopreservation of sturgeon spermatozoa.
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DOI: 10.1111/j.1439-0426.2011.01866.x
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Linhart</name><affiliation><mods:affiliation>Faculty of Fisheries and Protection of Water, Research Institute of Fish Culture and Hydrobiology, University of South Bohemia, Vodnany, Czech Republic</mods:affiliation></affiliation></author></analytic><monogr></monogr><series><title level="j">Journal of Applied Ichthyology</title><idno type="ISSN">0175-8659</idno><idno type="eISSN">1439-0426</idno><imprint><publisher>Blackwell Publishing Ltd</publisher><pubPlace>Oxford, UK</pubPlace><date type="published" when="2011-10">2011-10</date><biblScope unit="volume">27</biblScope><biblScope unit="issue">5</biblScope><biblScope unit="page" from="1147">1147</biblScope><biblScope unit="page" to="1149">1149</biblScope></imprint><idno type="ISSN">0175-8659</idno></series><idno type="istex">16E44A3D1E6841A6E53F3E36822E0D119D437C89</idno><idno type="DOI">10.1111/j.1439-0426.2011.01866.x</idno><idno type="ArticleID">JAI1866</idno></biblStruct></sourceDesc><seriesStmt><idno type="ISSN">0175-8659</idno></seriesStmt></fileDesc><profileDesc><textClass></textClass><langUsage><language ident="en">en</language></langUsage></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">The present study examined the effectiveness of different cryoprotectan uses for cryopreservation of sterlet (Acipenser ruthenus) sperm. Four different cryoprotectans [dimethyl‐sulfoxide (DMSO), dimethylacetamide (DMA), ethyleneglycol (EG) and methanol (MET)] in concentrations of 5 and 10% in the extender media (30 mm sucrose, 1 mm KCl, 25 mm Tris–HCl pH 8.5) were used for the cryopreservation of sperm from five sterlet males. Percentages of motility, velocity, fertilization and hatching rate were measured. The highest post‐thawing motility was observed in sperm samples cryopreserved with 10% MET (46 ± 19%), 10% DMA (47 ± 18%) and 10% DMSO (45 ± 7%). Fertilization rate in the control (fresh sperm) was 69 ± 9% and the hatching rate 61 ± 8%. A higher hatching rate after cryopreservation was obtained with 10% MET (32 ± 17%) and 5% DMA (23 ± 3%) when compared to data obtained with 10% DMA (9 ± 5%), 5% MET and 5 and 10% DMSO, wherein the hatching rate was around zero. Our results demonstrate that DMA can be used for cryopreservation of sturgeon spermatozoa.</div></front></TEI><istex><corpusName>wiley</corpusName><author><json:item><name>S. Boryshpolets</name><affiliations><json:string>Faculty of Fisheries and Protection of Water, Research Institute of Fish Culture and Hydrobiology, University of South Bohemia, Vodnany, Czech Republic</json:string></affiliations></json:item><json:item><name>B. Dzyuba</name><affiliations><json:string>Faculty of Fisheries and Protection of Water, Research Institute of Fish Culture and Hydrobiology, University of South Bohemia, Vodnany, Czech Republic</json:string><json:string>Institute for Problems of Cryobiology and Cryomedicine, National Academy of Sciences of the Ukraine, Kharkov, Ukraine</json:string></affiliations></json:item><json:item><name>M. 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The highest post‐thawing motility was observed in sperm samples cryopreserved with 10% MET (46 ± 19%), 10% DMA (47 ± 18%) and 10% DMSO (45 ± 7%). Fertilization rate in the control (fresh sperm) was 69 ± 9% and the hatching rate 61 ± 8%. A higher hatching rate after cryopreservation was obtained with 10% MET (32 ± 17%) and 5% DMA (23 ± 3%) when compared to data obtained with 10% DMA (9 ± 5%), 5% MET and 5 and 10% DMSO, wherein the hatching rate was around zero. 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Four different cryoprotectans [dimethyl‐sulfoxide (DMSO), dimethylacetamide (DMA), ethyleneglycol (EG) and methanol (MET)] in concentrations of 5 and 10% in the extender media (30 mm sucrose, 1 mm KCl, 25 mm Tris–HCl pH 8.5) were used for the cryopreservation of sperm from five sterlet males. Percentages of motility, velocity, fertilization and hatching rate were measured. The highest post‐thawing motility was observed in sperm samples cryopreserved with 10% MET (46 ± 19%), 10% DMA (47 ± 18%) and 10% DMSO (45 ± 7%). Fertilization rate in the control (fresh sperm) was 69 ± 9% and the hatching rate 61 ± 8%. A higher hatching rate after cryopreservation was obtained with 10% MET (32 ± 17%) and 5% DMA (23 ± 3%) when compared to data obtained with 10% DMA (9 ± 5%), 5% MET and 5 and 10% DMSO, wherein the hatching rate was around zero. Our results demonstrate that DMA can be used for cryopreservation of sturgeon spermatozoa.</p></abstract></profileDesc><revisionDesc><change when="2011-10">Published</change></revisionDesc></teiHeader></istex:fulltextTEI><json:item><extension>txt</extension><original>false</original><mimetype>text/plain</mimetype><uri>https://api.istex.fr/document/16E44A3D1E6841A6E53F3E36822E0D119D437C89/fulltext/txt</uri></json:item></fulltext><metadata><istex:metadataXml wicri:clean="Wiley, elements deleted: body"><istex:xmlDeclaration>version="1.0" encoding="UTF-8" standalone="yes"</istex:xmlDeclaration><istex:document><component version="2.0" type="serialArticle" xml:lang="en"><header><publicationMeta level="product"><publisherInfo><publisherName>Blackwell Publishing Ltd</publisherName><publisherLoc>Oxford, UK</publisherLoc></publisherInfo><doi origin="wiley" registered="yes">10.1111/(ISSN)1439-0426</doi><issn type="print">0175-8659</issn><issn type="electronic">1439-0426</issn><idGroup><id type="product" value="JAI"></id><id type="publisherDivision" value="ST"></id></idGroup><titleGroup><title type="main" sort="JOURNAL OF APPLIED ICHTHYOLOGY">Journal of Applied Ichthyology</title></titleGroup></publicationMeta><publicationMeta level="part" position="10105"><doi origin="wiley">10.1111/jai.2011.27.issue-5</doi><numberingGroup><numbering type="journalVolume" number="27">27</numbering><numbering type="journalIssue" number="5">5</numbering></numberingGroup><coverDate startDate="2011-10">October 2011</coverDate></publicationMeta><publicationMeta level="unit" type="article" position="2" status="forIssue"><doi origin="wiley">10.1111/j.1439-0426.2011.01866.x</doi><idGroup><id type="unit" value="JAI1866"></id></idGroup><countGroup><count type="pageTotal" number="3"></count></countGroup><titleGroup><title type="tocHeading1">Original articles</title></titleGroup><copyright>© 2011 Blackwell Verlag, Berlin</copyright><eventGroup><event type="xmlConverted" agent="Converter:BPG_TO_WML3G version:3.0 mode:FullText" date="2011-11-21"></event><event type="publishedOnlineEarlyUnpaginated" date="2011-08-04"></event><event type="publishedOnlineFinalForm" date="2011-09-14"></event><event type="firstOnline" date="2011-08-04"></event><event type="xmlConverted" agent="Converter:WILEY_ML3G_TO_WILEY_ML3GV2 version:3.8.8" date="2014-01-28"></event><event type="xmlConverted" agent="Converter:WML3G_To_WML3G version:4.1.7 mode:FullText,remove_FC" date="2014-10-24"></event></eventGroup><numberingGroup><numbering type="pageFirst" number="1147">1147</numbering><numbering type="pageLast" number="1149">1149</numbering></numberingGroup><correspondenceTo><b>Author’s address:</b> S. Boryshpolets, University of South Bohemia, Faculty of Fisheries and Protection of Water, Research Institute of Fish Culture and Hydrobiology, Zatisi 728/II, 389 25, Vodnany, Czech Republic.
E‐mail: <email>boryss00@vurh.jcu.cz</email></correspondenceTo><linkGroup><link type="toTypesetVersion" href="file:JAI.JAI1866.pdf"></link></linkGroup></publicationMeta><contentMeta><unparsedEditorialHistory>Received: April 10, 2011 Accepted: June 12, 2011</unparsedEditorialHistory><countGroup><count type="figureTotal" number="2"></count><count type="tableTotal" number="1"></count></countGroup><titleGroup><title type="main">Cryopreservation of sterlet (<i>Acipenser ruthenus</i>) spermatozoa using different cryoprotectants</title><title type="shortAuthors">S. Boryshpolets et al.</title><title type="short">Cryopreservation of sterlet (<i>Acipenser ruthenus</i>) spermatozoa</title></titleGroup><creators><creator creatorRole="author" xml:id="cr1" affiliationRef="#a1"><personName><givenNames>S.</givenNames><familyName>Boryshpolets</familyName></personName></creator><creator creatorRole="author" xml:id="cr2" affiliationRef="#a1 #a2"><personName><givenNames>B.</givenNames><familyName>Dzyuba</familyName></personName></creator><creator creatorRole="author" xml:id="cr3" affiliationRef="#a1"><personName><givenNames>M.</givenNames><familyName>Rodina</familyName></personName></creator><creator creatorRole="author" xml:id="cr4" affiliationRef="#a1"><personName><givenNames>S. M. H.</givenNames><familyName>Alavi</familyName></personName></creator><creator creatorRole="author" xml:id="cr5" affiliationRef="#a1"><personName><givenNames>D.</givenNames><familyName>Gela</familyName></personName></creator><creator creatorRole="author" xml:id="cr6" affiliationRef="#a1"><personName><givenNames>O.</givenNames><familyName>Linhart</familyName></personName></creator></creators><affiliationGroup><affiliation xml:id="a1" countryCode="CZ"><unparsedAffiliation>Faculty of Fisheries and Protection of Water, Research Institute of Fish Culture and Hydrobiology, University of South Bohemia, Vodnany, Czech Republic</unparsedAffiliation></affiliation><affiliation xml:id="a2" countryCode="UA"><unparsedAffiliation>Institute for Problems of Cryobiology and Cryomedicine, National Academy of Sciences of the Ukraine, Kharkov, Ukraine</unparsedAffiliation></affiliation></affiliationGroup><abstractGroup><abstract type="main" xml:lang="en"><title type="main">Summary</title><p>The present study examined the effectiveness of different cryoprotectan uses for cryopreservation of sterlet (<i>Acipenser ruthenus</i>) sperm. Four different cryoprotectans [dimethyl‐sulfoxide (DMSO), dimethylacetamide (DMA), ethyleneglycol (EG) and methanol (MET)] in concentrations of 5 and 10% in the extender media (30 m<sc>m</sc> sucrose, 1 m<sc>m</sc> KCl, 25 m<sc>m</sc> Tris–HCl pH 8.5) were used for the cryopreservation of sperm from five sterlet males. Percentages of motility, velocity, fertilization and hatching rate were measured. The highest post‐thawing motility was observed in sperm samples cryopreserved with 10% MET (46 ± 19%), 10% DMA (47 ± 18%) and 10% DMSO (45 ± 7%). Fertilization rate in the control (fresh sperm) was 69 ± 9% and the hatching rate 61 ± 8%. A higher hatching rate after cryopreservation was obtained with 10% MET (32 ± 17%) and 5% DMA (23 ± 3%) when compared to data obtained with 10% DMA (9 ± 5%), 5% MET and 5 and 10% DMSO, wherein the hatching rate was around zero. Our results demonstrate that DMA can be used for cryopreservation of sturgeon spermatozoa.</p></abstract></abstractGroup></contentMeta></header></component></istex:document></istex:metadataXml><mods version="3.6"><titleInfo lang="en"><title>Cryopreservation of sterlet (Acipenser ruthenus) spermatozoa using different cryoprotectants</title></titleInfo><titleInfo type="abbreviated" lang="en"><title>Cryopreservation of sterlet (Acipenser ruthenus) spermatozoa</title></titleInfo><titleInfo type="alternative" contentType="CDATA" lang="en"><title>Cryopreservation of sterlet (Acipenser ruthenus) spermatozoa using different cryoprotectants</title></titleInfo><name type="personal"><namePart type="given">S.</namePart><namePart type="family">Boryshpolets</namePart><affiliation>Faculty of Fisheries and Protection of Water, Research Institute of Fish Culture and Hydrobiology, University of South Bohemia, Vodnany, Czech Republic</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">B.</namePart><namePart type="family">Dzyuba</namePart><affiliation>Faculty of Fisheries and Protection of Water, Research Institute of Fish Culture and Hydrobiology, University of South Bohemia, Vodnany, Czech Republic</affiliation><affiliation>Institute for Problems of Cryobiology and Cryomedicine, National Academy of Sciences of the Ukraine, Kharkov, Ukraine</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">M.</namePart><namePart type="family">Rodina</namePart><affiliation>Faculty of Fisheries and Protection of Water, Research Institute of Fish Culture and Hydrobiology, University of South Bohemia, Vodnany, Czech Republic</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">S. M. H.</namePart><namePart type="family">Alavi</namePart><affiliation>Faculty of Fisheries and Protection of Water, Research Institute of Fish Culture and Hydrobiology, University of South Bohemia, Vodnany, Czech Republic</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">D.</namePart><namePart type="family">Gela</namePart><affiliation>Faculty of Fisheries and Protection of Water, Research Institute of Fish Culture and Hydrobiology, University of South Bohemia, Vodnany, Czech Republic</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">O.</namePart><namePart type="family">Linhart</namePart><affiliation>Faculty of Fisheries and Protection of Water, Research Institute of Fish Culture and Hydrobiology, University of South Bohemia, Vodnany, Czech Republic</affiliation><role><roleTerm type="text">author</roleTerm></role></name><typeOfResource>text</typeOfResource><genre type="article" displayLabel="article"></genre><originInfo><publisher>Blackwell Publishing Ltd</publisher><place><placeTerm type="text">Oxford, UK</placeTerm></place><dateIssued encoding="w3cdtf">2011-10</dateIssued><edition>Received: April 10, 2011 Accepted: June 12, 2011</edition><copyrightDate encoding="w3cdtf">2011</copyrightDate></originInfo><language><languageTerm type="code" authority="rfc3066">en</languageTerm><languageTerm type="code" authority="iso639-2b">eng</languageTerm></language><physicalDescription><internetMediaType>text/html</internetMediaType><extent unit="figures">2</extent><extent unit="tables">1</extent></physicalDescription><abstract lang="en">The present study examined the effectiveness of different cryoprotectan uses for cryopreservation of sterlet (Acipenser ruthenus) sperm. Four different cryoprotectans [dimethyl‐sulfoxide (DMSO), dimethylacetamide (DMA), ethyleneglycol (EG) and methanol (MET)] in concentrations of 5 and 10% in the extender media (30 mm sucrose, 1 mm KCl, 25 mm Tris–HCl pH 8.5) were used for the cryopreservation of sperm from five sterlet males. Percentages of motility, velocity, fertilization and hatching rate were measured. The highest post‐thawing motility was observed in sperm samples cryopreserved with 10% MET (46 ± 19%), 10% DMA (47 ± 18%) and 10% DMSO (45 ± 7%). Fertilization rate in the control (fresh sperm) was 69 ± 9% and the hatching rate 61 ± 8%. A higher hatching rate after cryopreservation was obtained with 10% MET (32 ± 17%) and 5% DMA (23 ± 3%) when compared to data obtained with 10% DMA (9 ± 5%), 5% MET and 5 and 10% DMSO, wherein the hatching rate was around zero. Our results demonstrate that DMA can be used for cryopreservation of sturgeon spermatozoa.</abstract><relatedItem type="host"><titleInfo><title>Journal of Applied Ichthyology</title></titleInfo><genre type="journal">journal</genre><identifier type="ISSN">0175-8659</identifier><identifier type="eISSN">1439-0426</identifier><identifier type="DOI">10.1111/(ISSN)1439-0426</identifier><identifier type="PublisherID">JAI</identifier><part><date>2011</date><detail type="volume"><caption>vol.</caption><number>27</number></detail><detail type="issue"><caption>no.</caption><number>5</number></detail><extent unit="pages"><start>1147</start><end>1149</end><total>3</total></extent></part></relatedItem><identifier type="istex">16E44A3D1E6841A6E53F3E36822E0D119D437C89</identifier><identifier type="DOI">10.1111/j.1439-0426.2011.01866.x</identifier><identifier type="ArticleID">JAI1866</identifier><accessCondition type="use and reproduction" contentType="copyright">© 2011 Blackwell Verlag, Berlin</accessCondition><recordInfo><recordContentSource>WILEY</recordContentSource><recordOrigin>Blackwell Publishing Ltd</recordOrigin></recordInfo></mods></metadata><serie></serie></istex></record>Pour manipuler ce document sous Unix (Dilib)
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