Physico‐chemical properties and protein profiles of sperm from three freshwater chondrostean species: a comparative study among Siberian sturgeon (Acipenser baerii), sterlet (Acipenser ruthenus) and paddlefish (Polyodon spathula)
Identifieur interne : 000651 ( Main/Merge ); précédent : 000650; suivant : 000652Physico‐chemical properties and protein profiles of sperm from three freshwater chondrostean species: a comparative study among Siberian sturgeon (Acipenser baerii), sterlet (Acipenser ruthenus) and paddlefish (Polyodon spathula)
Auteurs : P. Li [République tchèque, République populaire de Chine] ; M. Rodina [République tchèque] ; M. Hulak [République tchèque] ; D. Gela [République tchèque] ; M. Psenicka [République tchèque] ; Z. H. Li [République tchèque, République populaire de Chine] ; O. Linhart [République tchèque]Source :
- Journal of Applied Ichthyology [ 0175-8659 ] ; 2011-04.
Abstract
Physico‐chemical properties (spermatozoa motility, spermatozoa concentration, seminal plasma protein concentration, osmolality, pH, ions) and sperm protein profiles were evaluated in three Acipenseriforme species [Siberian sturgeon (Acipenser baerii), sterlet (Acipenser ruthenus) and paddlefish (Polyodon spathula)]. The spermatozoa velocity was observed significantly highest in sterlet and lowest in Siberian sturgeon (P < 0.05). Spermatozoa velocity was positively correlated to pH and the concentration of Na+ in seminal plasma in all species examined. Other tested parameters, in particular the concentration of K+, expressed species specific patterns, which could be linked to taxonomical status of species. The protein profiles of seminal plasma indicated a protein band with a molecular weight of 32.6 kDa exclusively detected in paddlefish. In spermatozoa, 95 protein spots were detected in Siberian sturgeon and sterlet, respectively, whereas only 70 spots were observed in paddlefish. Each spermatozoa protein map expressed a strong species specific pattern, which offers a basic benchmark to trace molecular differences among Acipenseriformes. It can be concluded that results presented in this study could be applied for successful reproduction management and cryopreservation protocols of these endangered species.
Url:
DOI: 10.1111/j.1439-0426.2010.01634.x
Links toward previous steps (curation, corpus...)
- to stream Istex, to step Corpus: 000697
- to stream Istex, to step Curation: 000695
- to stream Istex, to step Checkpoint: 000219
Links to Exploration step
ISTEX:D926A41A4B1E0FA3675F57E9BF81814D69960181Le document en format XML
<record><TEI wicri:istexFullTextTei="biblStruct"><teiHeader><fileDesc><titleStmt><title xml:lang="en">Physico‐chemical properties and protein profiles of sperm from three freshwater chondrostean species: a comparative study among Siberian sturgeon (Acipenser baerii), sterlet (Acipenser ruthenus) and paddlefish (Polyodon spathula)</title><author><name sortKey="Li, P" sort="Li, P" uniqKey="Li P" first="P." last="Li">P. Li</name></author><author><name sortKey="Rodina, M" sort="Rodina, M" uniqKey="Rodina M" first="M." last="Rodina">M. Rodina</name></author><author><name sortKey="Hulak, M" sort="Hulak, M" uniqKey="Hulak M" first="M." last="Hulak">M. Hulak</name></author><author><name sortKey="Gela, D" sort="Gela, D" uniqKey="Gela D" first="D." last="Gela">D. Gela</name></author><author><name sortKey="Psenicka, M" sort="Psenicka, M" uniqKey="Psenicka M" first="M." last="Psenicka">M. Psenicka</name></author><author><name sortKey="Li, Z H" sort="Li, Z H" uniqKey="Li Z" first="Z. H." last="Li">Z. H. Li</name></author><author><name sortKey="Linhart, O" sort="Linhart, O" uniqKey="Linhart O" first="O." last="Linhart">O. Linhart</name></author></titleStmt><publicationStmt><idno type="wicri:source">ISTEX</idno><idno type="RBID">ISTEX:D926A41A4B1E0FA3675F57E9BF81814D69960181</idno><date when="2011" year="2011">2011</date><idno type="doi">10.1111/j.1439-0426.2010.01634.x</idno><idno type="url">https://api.istex.fr/document/D926A41A4B1E0FA3675F57E9BF81814D69960181/fulltext/pdf</idno><idno type="wicri:Area/Istex/Corpus">000697</idno><idno type="wicri:explorRef" wicri:stream="Istex" wicri:step="Corpus" wicri:corpus="ISTEX">000697</idno><idno type="wicri:Area/Istex/Curation">000695</idno><idno type="wicri:Area/Istex/Checkpoint">000219</idno><idno type="wicri:explorRef" wicri:stream="Istex" wicri:step="Checkpoint">000219</idno><idno type="wicri:doubleKey">0175-8659:2011:Li P:physico:chemical:properties</idno><idno type="wicri:Area/Main/Merge">000651</idno></publicationStmt><sourceDesc><biblStruct><analytic><title level="a" type="main" xml:lang="en">Physico‐chemical properties and protein profiles of sperm from three freshwater chondrostean species: a comparative study among Siberian sturgeon (Acipenser baerii), sterlet (Acipenser ruthenus) and paddlefish (Polyodon spathula)</title><author><name sortKey="Li, P" sort="Li, P" uniqKey="Li P" first="P." last="Li">P. Li</name><affiliation wicri:level="1"><country xml:lang="fr">République tchèque</country><wicri:regionArea>South Bohemian Research Center of Aquaculture and Biodiversity of Hydrocenoses, Faculty of Fisheries and Protection of Waters, University of South Bohemia in Ceske Budejovice, Vodnany</wicri:regionArea><wicri:noRegion>Vodnany</wicri:noRegion></affiliation><affiliation wicri:level="1"><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Key Laboratory of Freshwater Biodiversity Conservation and Utilization, Ministry of Agriculture, Yangtze River Fisheries Research Institute, Chinese Academy of Fishery Science, Jingzhou, Hubei</wicri:regionArea><wicri:noRegion>Hubei</wicri:noRegion></affiliation></author><author><name sortKey="Rodina, M" sort="Rodina, M" uniqKey="Rodina M" first="M." last="Rodina">M. Rodina</name><affiliation wicri:level="1"><country xml:lang="fr">République tchèque</country><wicri:regionArea>South Bohemian Research Center of Aquaculture and Biodiversity of Hydrocenoses, Faculty of Fisheries and Protection of Waters, University of South Bohemia in Ceske Budejovice, Vodnany</wicri:regionArea><wicri:noRegion>Vodnany</wicri:noRegion></affiliation></author><author><name sortKey="Hulak, M" sort="Hulak, M" uniqKey="Hulak M" first="M." last="Hulak">M. Hulak</name><affiliation wicri:level="1"><country xml:lang="fr">République tchèque</country><wicri:regionArea>South Bohemian Research Center of Aquaculture and Biodiversity of Hydrocenoses, Faculty of Fisheries and Protection of Waters, University of South Bohemia in Ceske Budejovice, Vodnany</wicri:regionArea><wicri:noRegion>Vodnany</wicri:noRegion></affiliation></author><author><name sortKey="Gela, D" sort="Gela, D" uniqKey="Gela D" first="D." last="Gela">D. Gela</name><affiliation wicri:level="1"><country xml:lang="fr">République tchèque</country><wicri:regionArea>South Bohemian Research Center of Aquaculture and Biodiversity of Hydrocenoses, Faculty of Fisheries and Protection of Waters, University of South Bohemia in Ceske Budejovice, Vodnany</wicri:regionArea><wicri:noRegion>Vodnany</wicri:noRegion></affiliation></author><author><name sortKey="Psenicka, M" sort="Psenicka, M" uniqKey="Psenicka M" first="M." last="Psenicka">M. Psenicka</name><affiliation wicri:level="1"><country xml:lang="fr">République tchèque</country><wicri:regionArea>South Bohemian Research Center of Aquaculture and Biodiversity of Hydrocenoses, Faculty of Fisheries and Protection of Waters, University of South Bohemia in Ceske Budejovice, Vodnany</wicri:regionArea><wicri:noRegion>Vodnany</wicri:noRegion></affiliation></author><author><name sortKey="Li, Z H" sort="Li, Z H" uniqKey="Li Z" first="Z. H." last="Li">Z. H. Li</name><affiliation wicri:level="1"><country xml:lang="fr">République tchèque</country><wicri:regionArea>South Bohemian Research Center of Aquaculture and Biodiversity of Hydrocenoses, Faculty of Fisheries and Protection of Waters, University of South Bohemia in Ceske Budejovice, Vodnany</wicri:regionArea><wicri:noRegion>Vodnany</wicri:noRegion></affiliation><affiliation wicri:level="1"><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Key Laboratory of Freshwater Biodiversity Conservation and Utilization, Ministry of Agriculture, Yangtze River Fisheries Research Institute, Chinese Academy of Fishery Science, Jingzhou, Hubei</wicri:regionArea><wicri:noRegion>Hubei</wicri:noRegion></affiliation></author><author><name sortKey="Linhart, O" sort="Linhart, O" uniqKey="Linhart O" first="O." last="Linhart">O. Linhart</name><affiliation wicri:level="1"><country xml:lang="fr">République tchèque</country><wicri:regionArea>South Bohemian Research Center of Aquaculture and Biodiversity of Hydrocenoses, Faculty of Fisheries and Protection of Waters, University of South Bohemia in Ceske Budejovice, Vodnany</wicri:regionArea><wicri:noRegion>Vodnany</wicri:noRegion></affiliation></author></analytic><monogr></monogr><series><title level="j">Journal of Applied Ichthyology</title><idno type="ISSN">0175-8659</idno><idno type="eISSN">1439-0426</idno><imprint><publisher>Blackwell Publishing Ltd</publisher><pubPlace>Oxford, UK</pubPlace><date type="published" when="2011-04">2011-04</date><biblScope unit="volume">27</biblScope><biblScope unit="issue">2</biblScope><biblScope unit="page" from="673">673</biblScope><biblScope unit="page" to="677">677</biblScope></imprint><idno type="ISSN">0175-8659</idno></series><idno type="istex">D926A41A4B1E0FA3675F57E9BF81814D69960181</idno><idno type="DOI">10.1111/j.1439-0426.2010.01634.x</idno><idno type="ArticleID">JAI1634</idno></biblStruct></sourceDesc><seriesStmt><idno type="ISSN">0175-8659</idno></seriesStmt></fileDesc><profileDesc><textClass></textClass><langUsage><language ident="en">en</language></langUsage></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Physico‐chemical properties (spermatozoa motility, spermatozoa concentration, seminal plasma protein concentration, osmolality, pH, ions) and sperm protein profiles were evaluated in three Acipenseriforme species [Siberian sturgeon (Acipenser baerii), sterlet (Acipenser ruthenus) and paddlefish (Polyodon spathula)]. The spermatozoa velocity was observed significantly highest in sterlet and lowest in Siberian sturgeon (P < 0.05). Spermatozoa velocity was positively correlated to pH and the concentration of Na+ in seminal plasma in all species examined. Other tested parameters, in particular the concentration of K+, expressed species specific patterns, which could be linked to taxonomical status of species. The protein profiles of seminal plasma indicated a protein band with a molecular weight of 32.6 kDa exclusively detected in paddlefish. In spermatozoa, 95 protein spots were detected in Siberian sturgeon and sterlet, respectively, whereas only 70 spots were observed in paddlefish. Each spermatozoa protein map expressed a strong species specific pattern, which offers a basic benchmark to trace molecular differences among Acipenseriformes. It can be concluded that results presented in this study could be applied for successful reproduction management and cryopreservation protocols of these endangered species.</div></front></TEI></record>Pour manipuler ce document sous Unix (Dilib)
EXPLOR_STEP=$WICRI_ROOT/Wicri/Eau/explor/EsturgeonV1/Data/Main/Merge
HfdSelect -h $EXPLOR_STEP/biblio.hfd -nk 000651 | SxmlIndent | more
Ou
HfdSelect -h $EXPLOR_AREA/Data/Main/Merge/biblio.hfd -nk 000651 | SxmlIndent | more
Pour mettre un lien sur cette page dans le réseau Wicri
{{Explor lien
|wiki= Wicri/Eau
|area= EsturgeonV1
|flux= Main
|étape= Merge
|type= RBID
|clé= ISTEX:D926A41A4B1E0FA3675F57E9BF81814D69960181
|texte= Physico‐chemical properties and protein profiles of sperm from three freshwater chondrostean species: a comparative study among Siberian sturgeon (Acipenser baerii), sterlet (Acipenser ruthenus) and paddlefish (Polyodon spathula)
}}
| This area was generated with Dilib version V0.6.27. |  |