Optimization of enzyme-assisted extraction and characterization of collagen from Chinese sturgeon (Acipenser sturio Linnaeus) skin
Identifieur interne : 000272 ( Main/Merge ); précédent : 000271; suivant : 000273Optimization of enzyme-assisted extraction and characterization of collagen from Chinese sturgeon (Acipenser sturio Linnaeus) skin
Auteurs : Weiwei Feng ; Ting Zhao ; Ye Zhou ; Fang Li ; Ye Zou ; Shiqi Bai ; Wei Wang ; Liuqing Yang ; Xiangyang WuSource :
- Pharmacognosy Magazine [ 0973-1296 ] ; 2013.
Abstract
Sturgeon (
To determine the optimum conditions with the maximum yield of the pepsin-soluble collagen (PSC) extraction.
The conditions of the extraction were optimized using response surface methodology. The Box–Behnken design was used to evaluate the effects of the three independent variables (extraction time, enzyme concentration, and solid–liquid ratio) on the PSC yield of the sturgeon skin.
The optimal conditions were: solid–liquid ratio of 1:11.88, enzyme concentration of 2.42%, and extraction time of 6.45 h. The maximum yield of 86.69% of PSC was obtained under the optimal conditions. This value was not significantly different from the predicted value (87.4%) of the RSM (
The results of this study indicated that the production of PSC from sturgeon skin is feasible and beneficial. The patterns of sodium dodecyl sulfate-polyacrylamide gel electrophoretic patterns (SDS-PAGE) indicated that the sturgeon skin contains type I collagen, which is made of α-chain and β-chain. The infrared spectra of the collagens also indicated that pepsin hydrolysis does not affect the secondary structure of collagen, especially triple-helical structure.
Url:
DOI: 10.4103/0973-1296.117859
PubMed: 24143042
PubMed Central: 3798137
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<sourceDesc><biblStruct><analytic><title xml:lang="en" level="a" type="main">Optimization of enzyme-assisted extraction and characterization of collagen from Chinese sturgeon (<italic>Acipenser sturio Linnaeus</italic>
) skin</title>
<author><name sortKey="Feng, Weiwei" sort="Feng, Weiwei" uniqKey="Feng W" first="Weiwei" last="Feng">Weiwei Feng</name>
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<author><name sortKey="Zhao, Ting" sort="Zhao, Ting" uniqKey="Zhao T" first="Ting" last="Zhao">Ting Zhao</name>
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</author>
<author><name sortKey="Zhou, Ye" sort="Zhou, Ye" uniqKey="Zhou Y" first="Ye" last="Zhou">Ye Zhou</name>
<affiliation><nlm:aff id="aff2"><italic>School of Pharmacy, Jiangsu University, 301 Xuefu Rd., 212013 Zhenjiang, Jiangsu, China</italic>
</nlm:aff>
</affiliation>
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<author><name sortKey="Li, Fang" sort="Li, Fang" uniqKey="Li F" first="Fang" last="Li">Fang Li</name>
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<author><name sortKey="Zou, Ye" sort="Zou, Ye" uniqKey="Zou Y" first="Ye" last="Zou">Ye Zou</name>
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<author><name sortKey="Bai, Shiqi" sort="Bai, Shiqi" uniqKey="Bai S" first="Shiqi" last="Bai">Shiqi Bai</name>
<affiliation><nlm:aff id="aff2"><italic>School of Pharmacy, Jiangsu University, 301 Xuefu Rd., 212013 Zhenjiang, Jiangsu, China</italic>
</nlm:aff>
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<author><name sortKey="Wang, Wei" sort="Wang, Wei" uniqKey="Wang W" first="Wei" last="Wang">Wei Wang</name>
<affiliation><nlm:aff id="aff2"><italic>School of Pharmacy, Jiangsu University, 301 Xuefu Rd., 212013 Zhenjiang, Jiangsu, China</italic>
</nlm:aff>
</affiliation>
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<author><name sortKey="Yang, Liuqing" sort="Yang, Liuqing" uniqKey="Yang L" first="Liuqing" last="Yang">Liuqing Yang</name>
<affiliation><nlm:aff id="aff3"><italic>School of Chemistry and Chemical Engineering, Jiangsu University, 301 Xuefu Rd., 212013 Zhenjiang, Jiangsu, China</italic>
</nlm:aff>
</affiliation>
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<author><name sortKey="Wu, Xiangyang" sort="Wu, Xiangyang" uniqKey="Wu X" first="Xiangyang" last="Wu">Xiangyang Wu</name>
<affiliation><nlm:aff id="aff4"><italic>School of Environment, Jiangsu University, 301 Xuefu Rd., 212013 Zhenjiang, Jiangsu, China</italic>
</nlm:aff>
</affiliation>
</author>
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<series><title level="j">Pharmacognosy Magazine</title>
<idno type="ISSN">0973-1296</idno>
<idno type="eISSN">0976-4062</idno>
<imprint><date when="2013">2013</date>
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<front><div type="abstract" xml:lang="en"><sec id="st1"><title>Background:</title>
<p>Sturgeon (<italic>Acipenser sturio Linnaeus</italic>
) skin contains high amount of nutrients including unsaturated fatty acids and collagen. A pepsin-assisted extraction procedure was developed and optimized for the extraction of collagen from Chinese sturgeon (<italic>Acipenser sturio Linnaeus</italic>
) skins.</p>
</sec>
<sec id="st2"><title>Objective:</title>
<p>To determine the optimum conditions with the maximum yield of the pepsin-soluble collagen (PSC) extraction.</p>
</sec>
<sec id="st3"><title>Materials and Methods:</title>
<p>The conditions of the extraction were optimized using response surface methodology. The Box–Behnken design was used to evaluate the effects of the three independent variables (extraction time, enzyme concentration, and solid–liquid ratio) on the PSC yield of the sturgeon skin.</p>
</sec>
<sec id="st4"><title>Results:</title>
<p>The optimal conditions were: solid–liquid ratio of 1:11.88, enzyme concentration of 2.42%, and extraction time of 6.45 h. The maximum yield of 86.69% of PSC was obtained under the optimal conditions. This value was not significantly different from the predicted value (87.4%) of the RSM (<italic>P</italic>
< 0.05).</p>
</sec>
<sec id="st5"><title>Conclusion:</title>
<p>The results of this study indicated that the production of PSC from sturgeon skin is feasible and beneficial. The patterns of sodium dodecyl sulfate-polyacrylamide gel electrophoretic patterns (SDS-PAGE) indicated that the sturgeon skin contains type I collagen, which is made of α-chain and β-chain. The infrared spectra of the collagens also indicated that pepsin hydrolysis does not affect the secondary structure of collagen, especially triple-helical structure.</p>
</sec>
</div>
</front>
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