Optimization of enzyme-assisted extraction and characterization of collagen from Chinese sturgeon (Acipenser sturio Linnaeus) skin
Identifieur interne : 000510 ( Ncbi/Checkpoint ); précédent : 000509; suivant : 000511Optimization of enzyme-assisted extraction and characterization of collagen from Chinese sturgeon (Acipenser sturio Linnaeus) skin
Auteurs : Weiwei Feng ; Ting Zhao ; Ye Zhou ; Fang Li ; Ye Zou ; Shiqi Bai ; Wei Wang ; Liuqing Yang ; Xiangyang WuSource :
- Pharmacognosy Magazine [ 0973-1296 ] ; 2013.
Abstract
Sturgeon (
To determine the optimum conditions with the maximum yield of the pepsin-soluble collagen (PSC) extraction.
The conditions of the extraction were optimized using response surface methodology. The Box–Behnken design was used to evaluate the effects of the three independent variables (extraction time, enzyme concentration, and solid–liquid ratio) on the PSC yield of the sturgeon skin.
The optimal conditions were: solid–liquid ratio of 1:11.88, enzyme concentration of 2.42%, and extraction time of 6.45 h. The maximum yield of 86.69% of PSC was obtained under the optimal conditions. This value was not significantly different from the predicted value (87.4%) of the RSM (
The results of this study indicated that the production of PSC from sturgeon skin is feasible and beneficial. The patterns of sodium dodecyl sulfate-polyacrylamide gel electrophoretic patterns (SDS-PAGE) indicated that the sturgeon skin contains type I collagen, which is made of α-chain and β-chain. The infrared spectra of the collagens also indicated that pepsin hydrolysis does not affect the secondary structure of collagen, especially triple-helical structure.
Url:
DOI: 10.4103/0973-1296.117859
PubMed: 24143042
PubMed Central: 3798137
Affiliations:
Links toward previous steps (curation, corpus...)
- to stream Pmc, to step Corpus: 000051
- to stream Pmc, to step Curation: 000051
- to stream Pmc, to step Checkpoint: 000020
- to stream PubMed, to step Corpus: 000240
- to stream PubMed, to step Curation: 000240
- to stream PubMed, to step Checkpoint: 000240
- to stream Ncbi, to step Merge: 000510
- to stream Ncbi, to step Curation: 000510
Links to Exploration step
PMC:3798137Le document en format XML
<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">Optimization of enzyme-assisted extraction and characterization of collagen from Chinese sturgeon (<italic>Acipenser sturio Linnaeus</italic>) skin</title><author><name sortKey="Feng, Weiwei" sort="Feng, Weiwei" uniqKey="Feng W" first="Weiwei" last="Feng">Weiwei Feng</name><affiliation><nlm:aff id="aff1"></nlm:aff></affiliation></author><author><name sortKey="Zhao, Ting" sort="Zhao, Ting" uniqKey="Zhao T" first="Ting" last="Zhao">Ting Zhao</name><affiliation><nlm:aff id="aff1"></nlm:aff></affiliation></author><author><name sortKey="Zhou, Ye" sort="Zhou, Ye" uniqKey="Zhou Y" first="Ye" last="Zhou">Ye Zhou</name><affiliation><nlm:aff id="aff2"><italic>School of Pharmacy, Jiangsu University, 301 Xuefu Rd., 212013 Zhenjiang, Jiangsu, China</italic></nlm:aff></affiliation></author><author><name sortKey="Li, Fang" sort="Li, Fang" uniqKey="Li F" first="Fang" last="Li">Fang Li</name><affiliation><nlm:aff id="aff1"></nlm:aff></affiliation></author><author><name sortKey="Zou, Ye" sort="Zou, Ye" uniqKey="Zou Y" first="Ye" last="Zou">Ye Zou</name><affiliation><nlm:aff id="aff1"></nlm:aff></affiliation></author><author><name sortKey="Bai, Shiqi" sort="Bai, Shiqi" uniqKey="Bai S" first="Shiqi" last="Bai">Shiqi Bai</name><affiliation><nlm:aff id="aff2"><italic>School of Pharmacy, Jiangsu University, 301 Xuefu Rd., 212013 Zhenjiang, Jiangsu, China</italic></nlm:aff></affiliation></author><author><name sortKey="Wang, Wei" sort="Wang, Wei" uniqKey="Wang W" first="Wei" last="Wang">Wei Wang</name><affiliation><nlm:aff id="aff2"><italic>School of Pharmacy, Jiangsu University, 301 Xuefu Rd., 212013 Zhenjiang, Jiangsu, China</italic></nlm:aff></affiliation></author><author><name sortKey="Yang, Liuqing" sort="Yang, Liuqing" uniqKey="Yang L" first="Liuqing" last="Yang">Liuqing Yang</name><affiliation><nlm:aff id="aff3"><italic>School of Chemistry and Chemical Engineering, Jiangsu University, 301 Xuefu Rd., 212013 Zhenjiang, Jiangsu, China</italic></nlm:aff></affiliation></author><author><name sortKey="Wu, Xiangyang" sort="Wu, Xiangyang" uniqKey="Wu X" first="Xiangyang" last="Wu">Xiangyang Wu</name><affiliation><nlm:aff id="aff4"><italic>School of Environment, Jiangsu University, 301 Xuefu Rd., 212013 Zhenjiang, Jiangsu, China</italic></nlm:aff></affiliation></author></titleStmt><publicationStmt><idno type="wicri:source">PMC</idno><idno type="pmid">24143042</idno><idno type="pmc">3798137</idno><idno type="url">http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3798137</idno><idno type="RBID">PMC:3798137</idno><idno type="doi">10.4103/0973-1296.117859</idno><date when="2013">2013</date><idno type="wicri:Area/Pmc/Corpus">000051</idno><idno type="wicri:explorRef" wicri:stream="Pmc" wicri:step="Corpus" wicri:corpus="PMC">000051</idno><idno type="wicri:Area/Pmc/Curation">000051</idno><idno type="wicri:explorRef" wicri:stream="Pmc" wicri:step="Curation">000051</idno><idno type="wicri:Area/Pmc/Checkpoint">000020</idno><idno type="wicri:explorRef" wicri:stream="Pmc" wicri:step="Checkpoint">000020</idno><idno type="wicri:source">PubMed</idno><idno type="wicri:Area/PubMed/Corpus">000240</idno><idno type="wicri:explorRef" wicri:stream="PubMed" wicri:step="Corpus" wicri:corpus="PubMed">000240</idno><idno type="wicri:Area/PubMed/Curation">000240</idno><idno type="wicri:explorRef" wicri:stream="PubMed" wicri:step="Curation">000240</idno><idno type="wicri:Area/PubMed/Checkpoint">000240</idno><idno type="wicri:explorRef" wicri:stream="Checkpoint" wicri:step="PubMed">000240</idno><idno type="wicri:Area/Ncbi/Merge">000510</idno><idno type="wicri:Area/Ncbi/Curation">000510</idno><idno type="wicri:Area/Ncbi/Checkpoint">000510</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en" level="a" type="main">Optimization of enzyme-assisted extraction and characterization of collagen from Chinese sturgeon (<italic>Acipenser sturio Linnaeus</italic>) skin</title><author><name sortKey="Feng, Weiwei" sort="Feng, Weiwei" uniqKey="Feng W" first="Weiwei" last="Feng">Weiwei Feng</name><affiliation><nlm:aff id="aff1"></nlm:aff></affiliation></author><author><name sortKey="Zhao, Ting" sort="Zhao, Ting" uniqKey="Zhao T" first="Ting" last="Zhao">Ting Zhao</name><affiliation><nlm:aff id="aff1"></nlm:aff></affiliation></author><author><name sortKey="Zhou, Ye" sort="Zhou, Ye" uniqKey="Zhou Y" first="Ye" last="Zhou">Ye Zhou</name><affiliation><nlm:aff id="aff2"><italic>School of Pharmacy, Jiangsu University, 301 Xuefu Rd., 212013 Zhenjiang, Jiangsu, China</italic></nlm:aff></affiliation></author><author><name sortKey="Li, Fang" sort="Li, Fang" uniqKey="Li F" first="Fang" last="Li">Fang Li</name><affiliation><nlm:aff id="aff1"></nlm:aff></affiliation></author><author><name sortKey="Zou, Ye" sort="Zou, Ye" uniqKey="Zou Y" first="Ye" last="Zou">Ye Zou</name><affiliation><nlm:aff id="aff1"></nlm:aff></affiliation></author><author><name sortKey="Bai, Shiqi" sort="Bai, Shiqi" uniqKey="Bai S" first="Shiqi" last="Bai">Shiqi Bai</name><affiliation><nlm:aff id="aff2"><italic>School of Pharmacy, Jiangsu University, 301 Xuefu Rd., 212013 Zhenjiang, Jiangsu, China</italic></nlm:aff></affiliation></author><author><name sortKey="Wang, Wei" sort="Wang, Wei" uniqKey="Wang W" first="Wei" last="Wang">Wei Wang</name><affiliation><nlm:aff id="aff2"><italic>School of Pharmacy, Jiangsu University, 301 Xuefu Rd., 212013 Zhenjiang, Jiangsu, China</italic></nlm:aff></affiliation></author><author><name sortKey="Yang, Liuqing" sort="Yang, Liuqing" uniqKey="Yang L" first="Liuqing" last="Yang">Liuqing Yang</name><affiliation><nlm:aff id="aff3"><italic>School of Chemistry and Chemical Engineering, Jiangsu University, 301 Xuefu Rd., 212013 Zhenjiang, Jiangsu, China</italic></nlm:aff></affiliation></author><author><name sortKey="Wu, Xiangyang" sort="Wu, Xiangyang" uniqKey="Wu X" first="Xiangyang" last="Wu">Xiangyang Wu</name><affiliation><nlm:aff id="aff4"><italic>School of Environment, Jiangsu University, 301 Xuefu Rd., 212013 Zhenjiang, Jiangsu, China</italic></nlm:aff></affiliation></author></analytic><series><title level="j">Pharmacognosy Magazine</title><idno type="ISSN">0973-1296</idno><idno type="eISSN">0976-4062</idno><imprint><date when="2013">2013</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en"><sec id="st1"><title>Background:</title><p>Sturgeon (<italic>Acipenser sturio Linnaeus</italic>) skin contains high amount of nutrients including unsaturated fatty acids and collagen. A pepsin-assisted extraction procedure was developed and optimized for the extraction of collagen from Chinese sturgeon (<italic>Acipenser sturio Linnaeus</italic>) skins.</p></sec><sec id="st2"><title>Objective:</title><p>To determine the optimum conditions with the maximum yield of the pepsin-soluble collagen (PSC) extraction.</p></sec><sec id="st3"><title>Materials and Methods:</title><p>The conditions of the extraction were optimized using response surface methodology. The Box–Behnken design was used to evaluate the effects of the three independent variables (extraction time, enzyme concentration, and solid–liquid ratio) on the PSC yield of the sturgeon skin.</p></sec><sec id="st4"><title>Results:</title><p>The optimal conditions were: solid–liquid ratio of 1:11.88, enzyme concentration of 2.42%, and extraction time of 6.45 h. The maximum yield of 86.69% of PSC was obtained under the optimal conditions. This value was not significantly different from the predicted value (87.4%) of the RSM (<italic>P</italic> < 0.05).</p></sec><sec id="st5"><title>Conclusion:</title><p>The results of this study indicated that the production of PSC from sturgeon skin is feasible and beneficial. The patterns of sodium dodecyl sulfate-polyacrylamide gel electrophoretic patterns (SDS-PAGE) indicated that the sturgeon skin contains type I collagen, which is made of α-chain and β-chain. The infrared spectra of the collagens also indicated that pepsin hydrolysis does not affect the secondary structure of collagen, especially triple-helical structure.</p></sec></div></front><back><div1 type="bibliography"><listBibl><biblStruct><analytic><author><name sortKey="Bai, J" uniqKey="Bai J">J Bai</name></author><author><name sortKey="Ma, D" uniqKey="Ma D">D Ma</name></author><author><name sortKey="Lao, H" uniqKey="Lao H">H Lao</name></author><author><name sortKey="Jian, Q" uniqKey="Jian Q">Q Jian</name></author><author><name sortKey="Ye, X" uniqKey="Ye X">X Ye</name></author><author><name sortKey="Luo, J" uniqKey="Luo J">J Luo</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Bo, Cheng Yh" uniqKey="Bo C">Cheng YH Bo</name></author><author><name sortKey="Zhong, Lv" uniqKey="Zhong L">LV Zhong</name></author><author><name sortKey="Qiang, D" uniqKey="Qiang D">D Qiang</name></author><author><name sortKey="Hongyan, Z" uniqKey="Hongyan Z">Z Hongyan</name></author><author><name sortKey="Fenfen, Fu" uniqKey="Fenfen F">FU Fenfen</name></author><author><name sortKey="Zeqiao, L" uniqKey="Zeqiao L">L Zeqiao</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Hao, S" uniqKey="Hao S">S Hao</name></author><author><name sortKey="Li, L" uniqKey="Li L">L Li</name></author><author><name sortKey="Yang, X" uniqKey="Yang X">X Yang</name></author><author><name sortKey="Cen, J" uniqKey="Cen J">J Cen</name></author><author><name sortKey="Shi, H" uniqKey="Shi H">H Shi</name></author><author><name sortKey="Bo, Q" uniqKey="Bo Q">Q Bo</name></author><author><name sortKey="He, J" uniqKey="He J">J He</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Kittiphattanabawon, P" uniqKey="Kittiphattanabawon P">P Kittiphattanabawon</name></author><author><name sortKey="Benjakul, S" uniqKey="Benjakul S">S Benjakul</name></author><author><name sortKey="Visessanguan, W" uniqKey="Visessanguan W">W Visessanguan</name></author><author><name sortKey="Kishimura, H" uniqKey="Kishimura H">H Kishimura</name></author><author><name sortKey="Shahidi, F" uniqKey="Shahidi F">F Shahidi</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Guo, J" uniqKey="Guo J">J Guo</name></author><author><name sortKey="Luo, Ye" uniqKey="Luo Y">Ye Luo</name></author><author><name sortKey="Fan, D" uniqKey="Fan D">D Fan</name></author><author><name sortKey="Gao, P" uniqKey="Gao P">P Gao</name></author><author><name sortKey="Ma, X" uniqKey="Ma X">X Ma</name></author><author><name sortKey="Zhu, C" uniqKey="Zhu C">C Zhu</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Zhang, C" uniqKey="Zhang C">C Zhang</name></author><author><name sortKey="Fan, D" uniqKey="Fan D">D Fan</name></author><author><name sortKey="Shang, La" uniqKey="Shang L">La Shang</name></author><author><name sortKey="Ma, X" uniqKey="Ma X">X Ma</name></author><author><name sortKey="Luo, Ye" uniqKey="Luo Y">Ye Luo</name></author><author><name sortKey="Xue, W" uniqKey="Xue W">W Xue</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Singh, P" uniqKey="Singh P">P Singh</name></author><author><name sortKey="Benjakul, S" uniqKey="Benjakul S">S Benjakul</name></author><author><name sortKey="Maqsood, S" uniqKey="Maqsood S">S Maqsood</name></author><author><name sortKey="Kishimura, H" uniqKey="Kishimura H">H Kishimura</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Nalinanon, S" uniqKey="Nalinanon S">S Nalinanon</name></author><author><name sortKey="Benjakul, S" uniqKey="Benjakul S">S Benjakul</name></author><author><name sortKey="Kishimura, H" uniqKey="Kishimura H">H Kishimura</name></author><author><name sortKey="Osako, K" uniqKey="Osako K">K Osako</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Triveni, R" uniqKey="Triveni R">R Triveni</name></author><author><name sortKey="Shamala, Tr" uniqKey="Shamala T">TR Shamala</name></author><author><name sortKey="Rastogi, Nk" uniqKey="Rastogi N">NK Rastogi</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Tang, Xh" uniqKey="Tang X">XH Tang</name></author><author><name sortKey="Yan, Lf" uniqKey="Yan L">LF Yan</name></author><author><name sortKey="Gao, J" uniqKey="Gao J">J Gao</name></author><author><name sortKey="Ge, Hy" uniqKey="Ge H">HY Ge</name></author><author><name sortKey="Yang, Hd" uniqKey="Yang H">HD Yang</name></author><author><name sortKey="Lin, N" uniqKey="Lin N">N Lin</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Yang, B" uniqKey="Yang B">B Yang</name></author><author><name sortKey="Zhao, M" uniqKey="Zhao M">M Zhao</name></author><author><name sortKey="Shi, J" uniqKey="Shi J">J Shi</name></author><author><name sortKey="Yang, N" uniqKey="Yang N">N Yang</name></author><author><name sortKey="Jiang, Y" uniqKey="Jiang Y">Y Jiang</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Wang, L" uniqKey="Wang L">L Wang</name></author><author><name sortKey="Yang, B" uniqKey="Yang B">B Yang</name></author><author><name sortKey="Du, X" uniqKey="Du X">X Du</name></author><author><name sortKey="Yi, C" uniqKey="Yi C">C Yi</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Suphatharaprateep, W" uniqKey="Suphatharaprateep W">W Suphatharaprateep</name></author><author><name sortKey="Cheirsilp, B" uniqKey="Cheirsilp B">B Cheirsilp</name></author><author><name sortKey="Jongjareonrak, A" uniqKey="Jongjareonrak A">A Jongjareonrak</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Cao, H" uniqKey="Cao H">H Cao</name></author><author><name sortKey="Xu, S Y" uniqKey="Xu S">S-Y Xu</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Xu, R" uniqKey="Xu R">R Xu</name></author><author><name sortKey="Chen, Yj" uniqKey="Chen Y">YJ Chen</name></author><author><name sortKey="Wan, Dr" uniqKey="Wan D">DR Wan</name></author><author><name sortKey="Wang, J" uniqKey="Wang J">J Wang</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Liyana Pathirana, C" uniqKey="Liyana Pathirana C">C Liyana-Pathirana</name></author><author><name sortKey="Shahidi, F" uniqKey="Shahidi F">F Shahidi</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Nath, A" uniqKey="Nath A">A Nath</name></author><author><name sortKey="Chattopadhyay, Pk" uniqKey="Chattopadhyay P">PK Chattopadhyay</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Sin, Hn" uniqKey="Sin H">HN Sin</name></author><author><name sortKey="Yusof, S" uniqKey="Yusof S">S Yusof</name></author><author><name sortKey="Sheikh, Abdul Hamid N" uniqKey="Sheikh A">Abdul Hamid N Sheikh</name></author><author><name sortKey="Rahman, Ra" uniqKey="Rahman R">RA Rahman</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Rosenthal, A" uniqKey="Rosenthal A">A Rosenthal</name></author><author><name sortKey="Pyle, Dl" uniqKey="Pyle D">DL Pyle</name></author><author><name sortKey="Niranjan, K" uniqKey="Niranjan K">K Niranjan</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Muyonga, Jh" uniqKey="Muyonga J">JH Muyonga</name></author><author><name sortKey="Cole, Cgb" uniqKey="Cole C">CGB Cole</name></author><author><name sortKey="Duodu, Kg" uniqKey="Duodu K">KG Duodu</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Kittiphattanabawon, P" uniqKey="Kittiphattanabawon P">P Kittiphattanabawon</name></author><author><name sortKey="Benjakul, S" uniqKey="Benjakul S">S Benjakul</name></author><author><name sortKey="Visessanguan, W" uniqKey="Visessanguan W">W Visessanguan</name></author><author><name sortKey="Nagai, T" uniqKey="Nagai T">T Nagai</name></author><author><name sortKey="Tanaka, M" uniqKey="Tanaka M">M Tanaka</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Friess, W" uniqKey="Friess W">W Friess</name></author><author><name sortKey="Lee, G" uniqKey="Lee G">G Lee</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Muyonga, Jh" uniqKey="Muyonga J">JH Muyonga</name></author><author><name sortKey="Cole, Cg" uniqKey="Cole C">CG Cole</name></author><author><name sortKey="Duodu, Kg" uniqKey="Duodu K">KG Duodu</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Abe, Y" uniqKey="Abe Y">Y Abe</name></author><author><name sortKey="Krimm, S" uniqKey="Krimm S">S Krimm</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Payne, Kj" uniqKey="Payne K">KJ Payne</name></author><author><name sortKey="Veis, A" uniqKey="Veis A">A Veis</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Surewicz, Wk" uniqKey="Surewicz W">WK Surewicz</name></author><author><name sortKey="Mantsh, H H" uniqKey="Mantsh H">H.H Mantsh</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Krimm, S" uniqKey="Krimm S">S Krimm</name></author><author><name sortKey="Bandekar, J" uniqKey="Bandekar J">J Bandekar</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Nagai, T" uniqKey="Nagai T">T Nagai</name></author><author><name sortKey="Suzuki, N" uniqKey="Suzuki N">N Suzuki</name></author></analytic></biblStruct></listBibl></div1></back></TEI><affiliations><list></list><tree><noCountry><name sortKey="Bai, Shiqi" sort="Bai, Shiqi" uniqKey="Bai S" first="Shiqi" last="Bai">Shiqi Bai</name><name sortKey="Feng, Weiwei" sort="Feng, Weiwei" uniqKey="Feng W" first="Weiwei" last="Feng">Weiwei Feng</name><name sortKey="Li, Fang" sort="Li, Fang" uniqKey="Li F" first="Fang" last="Li">Fang Li</name><name sortKey="Wang, Wei" sort="Wang, Wei" uniqKey="Wang W" first="Wei" last="Wang">Wei Wang</name><name sortKey="Wu, Xiangyang" sort="Wu, Xiangyang" uniqKey="Wu X" first="Xiangyang" last="Wu">Xiangyang Wu</name><name sortKey="Yang, Liuqing" sort="Yang, Liuqing" uniqKey="Yang L" first="Liuqing" last="Yang">Liuqing Yang</name><name sortKey="Zhao, Ting" sort="Zhao, Ting" uniqKey="Zhao T" first="Ting" last="Zhao">Ting Zhao</name><name sortKey="Zhou, Ye" sort="Zhou, Ye" uniqKey="Zhou Y" first="Ye" last="Zhou">Ye Zhou</name><name sortKey="Zou, Ye" sort="Zou, Ye" uniqKey="Zou Y" first="Ye" last="Zou">Ye Zou</name></noCountry></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
EXPLOR_STEP=$WICRI_ROOT/Wicri/Eau/explor/EsturgeonV1/Data/Ncbi/Checkpoint
HfdSelect -h $EXPLOR_STEP/biblio.hfd -nk 000510 | SxmlIndent | more
Ou
HfdSelect -h $EXPLOR_AREA/Data/Ncbi/Checkpoint/biblio.hfd -nk 000510 | SxmlIndent | more
Pour mettre un lien sur cette page dans le réseau Wicri
{{Explor lien
|wiki= Wicri/Eau
|area= EsturgeonV1
|flux= Ncbi
|étape= Checkpoint
|type= RBID
|clé= PMC:3798137
|texte= Optimization of enzyme-assisted extraction and characterization of collagen from Chinese sturgeon (Acipenser sturio Linnaeus) skin
}}
Pour générer des pages wiki
HfdIndexSelect -h $EXPLOR_AREA/Data/Ncbi/Checkpoint/RBID.i -Sk "pubmed:24143042" \
| HfdSelect -Kh $EXPLOR_AREA/Data/Ncbi/Checkpoint/biblio.hfd \
| NlmPubMed2Wicri -a EsturgeonV1
| This area was generated with Dilib version V0.6.27. |  |