Microsatellite multiplex assay for the analysis of Atlantic sturgeon populations
Identifieur interne : 000182 ( Main/Merge ); précédent : 000181; suivant : 000183Microsatellite multiplex assay for the analysis of Atlantic sturgeon populations
Auteurs : H. Panagiotopoulou [Pologne] ; D. Popovic [Pologne] ; K. Zalewska [Pologne] ; P. Weglenski [Pologne] ; A. Stankovic [Pologne]Source :
- Journal of Applied Genetics [ 1234-1983 ] ; 2014.
Abstract
We have developed a multiplex assay covering 16 microsatellite loci, amplified in four polymerase chain reaction (PCR) assays, and loaded on the ABI DNA Analyzer in two separate panels. The assay was tested on 603 individuals originating from wild populations and hatchery stocks of Atlantic sturgeon. The assay was also tested on 12 individuals of European sturgeon and appeared to be almost equally useful. The multiplex assay designed in this study can be successfully applied in studies requiring high genetic resolution, such as relatedness analysis, selective breeding programs, and stock identification of Atlantic sturgeon.
The online version of this article (doi:10.1007/s13353-014-0216-y) contains supplementary material, which is available to authorized users.
Url:
DOI: 10.1007/s13353-014-0216-y
PubMed: 24793446
PubMed Central: 4185099
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Stankovic</name><affiliation wicri:level="1"><nlm:aff id="Aff1">Institute of Biochemistry and Biophysics, Polish Academy of Sciences, Pawińskiego 5a, 02-106 Warsaw, Poland</nlm:aff><country xml:lang="fr">Pologne</country><wicri:regionArea>Institute of Biochemistry and Biophysics, Polish Academy of Sciences, Pawińskiego 5a, 02-106 Warsaw</wicri:regionArea><wicri:noRegion>02-106 Warsaw</wicri:noRegion></affiliation><affiliation wicri:level="1"><nlm:aff id="Aff3">Faculty of Biology, Institute of Genetics and Biotechnology, University of Warsaw, Pawińskiego 5a, 02-106 Warsaw, Poland</nlm:aff><country xml:lang="fr">Pologne</country><wicri:regionArea>Faculty of Biology, Institute of Genetics and Biotechnology, University of Warsaw, Pawińskiego 5a, 02-106 Warsaw</wicri:regionArea><wicri:noRegion>02-106 Warsaw</wicri:noRegion></affiliation><affiliation wicri:level="1"><nlm:aff id="Aff4">The Antiquity of Southeastern Europe Research Center, University of Warsaw, Krakowskie Przedmieście 32, 00-927 Warsaw, Poland</nlm:aff><country xml:lang="fr">Pologne</country><wicri:regionArea>The Antiquity of Southeastern Europe Research Center, University of Warsaw, Krakowskie Przedmieście 32, 00-927 Warsaw</wicri:regionArea><wicri:noRegion>00-927 Warsaw</wicri:noRegion></affiliation></author></analytic><series><title level="j">Journal of Applied Genetics</title><idno type="ISSN">1234-1983</idno><idno type="eISSN">2190-3883</idno><imprint><date when="2014">2014</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en"><p>We have developed a multiplex assay covering 16 microsatellite loci, amplified in four polymerase chain reaction (PCR) assays, and loaded on the ABI DNA Analyzer in two separate panels. The assay was tested on 603 individuals originating from wild populations and hatchery stocks of Atlantic sturgeon. The assay was also tested on 12 individuals of European sturgeon and appeared to be almost equally useful. The multiplex assay designed in this study can be successfully applied in studies requiring high genetic resolution, such as relatedness analysis, selective breeding programs, and stock identification of Atlantic sturgeon.</p><sec><title>Electronic supplementary material</title><p>The online version of this article (doi:10.1007/s13353-014-0216-y) contains supplementary material, which is available to authorized users.</p></sec></div></front><back><div1 type="bibliography"><listBibl><biblStruct><analytic><author><name sortKey="Blouin, Ms" uniqKey="Blouin M">MS Blouin</name></author><author><name sortKey="Parsons, M" uniqKey="Parsons M">M Parsons</name></author><author><name sortKey="Lacaille, V" uniqKey="Lacaille V">V Lacaille</name></author><author><name sortKey="Lotz, S" uniqKey="Lotz S">S Lotz</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Brookfield, Jfy" uniqKey="Brookfield J">JFY 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sortKey="King, T" uniqKey="King T">T King</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Wirgin, I" uniqKey="Wirgin I">I Wirgin</name></author><author><name sortKey="Maceda, L" uniqKey="Maceda L">L Maceda</name></author><author><name sortKey="Waldman, Jr" uniqKey="Waldman J">JR Waldman</name></author><author><name sortKey="Wehrell, S" uniqKey="Wehrell S">S Wehrell</name></author><author><name sortKey="Dadswell, D" uniqKey="Dadswell D">D Dadswell</name></author><author><name sortKey="King, T" uniqKey="King T">T King</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Zhu, Zy" uniqKey="Zhu Z">ZY Zhu</name></author><author><name sortKey="Wang, Cm" uniqKey="Wang C">CM Wang</name></author><author><name sortKey="Lo, Lc" uniqKey="Lo L">LC Lo</name></author><author><name sortKey="Lin, G" uniqKey="Lin G">G Lin</name></author><author><name sortKey="Feng, F" uniqKey="Feng F">F Feng</name></author><author><name sortKey="Tan, J" uniqKey="Tan J">J Tan</name></author><author><name sortKey="Chou, R" uniqKey="Chou R">R Chou</name></author><author><name sortKey="Lim, Hs" uniqKey="Lim H">HS Lim</name></author><author><name sortKey="Orban, L" uniqKey="Orban L">L Orban</name></author><author><name sortKey="Yue, Gh" uniqKey="Yue G">GH Yue</name></author></analytic></biblStruct></listBibl></div1></back></TEI></record>Pour manipuler ce document sous Unix (Dilib)
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