Microsatellite multiplex assay for the analysis of Atlantic sturgeon populations
Identifieur interne : 000012 ( Pmc/Checkpoint ); précédent : 000011; suivant : 000013Microsatellite multiplex assay for the analysis of Atlantic sturgeon populations
Auteurs : H. Panagiotopoulou [Pologne] ; D. Popovic [Pologne] ; K. Zalewska [Pologne] ; P. Weglenski [Pologne] ; A. Stankovic [Pologne]Source :
- Journal of Applied Genetics [ 1234-1983 ] ; 2014.
Abstract
We have developed a multiplex assay covering 16 microsatellite loci, amplified in four polymerase chain reaction (PCR) assays, and loaded on the ABI DNA Analyzer in two separate panels. The assay was tested on 603 individuals originating from wild populations and hatchery stocks of Atlantic sturgeon. The assay was also tested on 12 individuals of European sturgeon and appeared to be almost equally useful. The multiplex assay designed in this study can be successfully applied in studies requiring high genetic resolution, such as relatedness analysis, selective breeding programs, and stock identification of Atlantic sturgeon.
The online version of this article (doi:10.1007/s13353-014-0216-y) contains supplementary material, which is available to authorized users.
Url:
DOI: 10.1007/s13353-014-0216-y
PubMed: 24793446
PubMed Central: 4185099
Affiliations:
Links toward previous steps (curation, corpus...)
Links to Exploration step
PMC:4185099Le document en format XML
<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">Microsatellite multiplex assay for the analysis of Atlantic sturgeon populations</title><author><name sortKey="Panagiotopoulou, H" sort="Panagiotopoulou, H" uniqKey="Panagiotopoulou H" first="H." last="Panagiotopoulou">H. Panagiotopoulou</name><affiliation wicri:level="1"><nlm:aff id="Aff1">Institute of Biochemistry and Biophysics, Polish Academy of Sciences, Pawińskiego 5a, 02-106 Warsaw, Poland</nlm:aff><country xml:lang="fr">Pologne</country><wicri:regionArea>Institute of Biochemistry and Biophysics, Polish Academy of Sciences, Pawińskiego 5a, 02-106 Warsaw</wicri:regionArea><wicri:noRegion>02-106 Warsaw</wicri:noRegion></affiliation><affiliation wicri:level="1"><nlm:aff id="Aff5">Institute of Genetics and Biotechnology, University of Warsaw, Pawińskiego 5a, 02-106 Warsaw, Poland</nlm:aff><country xml:lang="fr">Pologne</country><wicri:regionArea>Institute of Genetics and Biotechnology, University of Warsaw, Pawińskiego 5a, 02-106 Warsaw</wicri:regionArea><wicri:noRegion>02-106 Warsaw</wicri:noRegion></affiliation></author><author><name sortKey="Popovic, D" sort="Popovic, D" uniqKey="Popovic D" first="D." last="Popovic">D. Popovic</name><affiliation wicri:level="1"><nlm:aff id="Aff2">Centre of New Technologies (CeNT), University of Warsaw, ul. Żwirki i Wigury 93, 02-089 Warsaw, Poland</nlm:aff><country xml:lang="fr">Pologne</country><wicri:regionArea>Centre of New Technologies (CeNT), University of Warsaw, ul. Żwirki i Wigury 93, 02-089 Warsaw</wicri:regionArea><wicri:noRegion>02-089 Warsaw</wicri:noRegion></affiliation></author><author><name sortKey="Zalewska, K" sort="Zalewska, K" uniqKey="Zalewska K" first="K." last="Zalewska">K. Zalewska</name><affiliation wicri:level="1"><nlm:aff id="Aff3">Faculty of Biology, Institute of Genetics and Biotechnology, University of Warsaw, Pawińskiego 5a, 02-106 Warsaw, Poland</nlm:aff><country xml:lang="fr">Pologne</country><wicri:regionArea>Faculty of Biology, Institute of Genetics and Biotechnology, University of Warsaw, Pawińskiego 5a, 02-106 Warsaw</wicri:regionArea><wicri:noRegion>02-106 Warsaw</wicri:noRegion></affiliation></author><author><name sortKey="Weglenski, P" sort="Weglenski, P" uniqKey="Weglenski P" first="P." last="Weglenski">P. Weglenski</name><affiliation wicri:level="1"><nlm:aff id="Aff1">Institute of Biochemistry and Biophysics, Polish Academy of Sciences, Pawińskiego 5a, 02-106 Warsaw, Poland</nlm:aff><country xml:lang="fr">Pologne</country><wicri:regionArea>Institute of Biochemistry and Biophysics, Polish Academy of Sciences, Pawińskiego 5a, 02-106 Warsaw</wicri:regionArea><wicri:noRegion>02-106 Warsaw</wicri:noRegion></affiliation><affiliation wicri:level="1"><nlm:aff id="Aff2">Centre of New Technologies (CeNT), University of Warsaw, ul. Żwirki i Wigury 93, 02-089 Warsaw, Poland</nlm:aff><country xml:lang="fr">Pologne</country><wicri:regionArea>Centre of New Technologies (CeNT), University of Warsaw, ul. Żwirki i Wigury 93, 02-089 Warsaw</wicri:regionArea><wicri:noRegion>02-089 Warsaw</wicri:noRegion></affiliation></author><author><name sortKey="Stankovic, A" sort="Stankovic, A" uniqKey="Stankovic A" first="A." last="Stankovic">A. Stankovic</name><affiliation wicri:level="1"><nlm:aff id="Aff1">Institute of Biochemistry and Biophysics, Polish Academy of Sciences, Pawińskiego 5a, 02-106 Warsaw, Poland</nlm:aff><country xml:lang="fr">Pologne</country><wicri:regionArea>Institute of Biochemistry and Biophysics, Polish Academy of Sciences, Pawińskiego 5a, 02-106 Warsaw</wicri:regionArea><wicri:noRegion>02-106 Warsaw</wicri:noRegion></affiliation><affiliation wicri:level="1"><nlm:aff id="Aff3">Faculty of Biology, Institute of Genetics and Biotechnology, University of Warsaw, Pawińskiego 5a, 02-106 Warsaw, Poland</nlm:aff><country xml:lang="fr">Pologne</country><wicri:regionArea>Faculty of Biology, Institute of Genetics and Biotechnology, University of Warsaw, Pawińskiego 5a, 02-106 Warsaw</wicri:regionArea><wicri:noRegion>02-106 Warsaw</wicri:noRegion></affiliation><affiliation wicri:level="1"><nlm:aff id="Aff4">The Antiquity of Southeastern Europe Research Center, University of Warsaw, Krakowskie Przedmieście 32, 00-927 Warsaw, Poland</nlm:aff><country xml:lang="fr">Pologne</country><wicri:regionArea>The Antiquity of Southeastern Europe Research Center, University of Warsaw, Krakowskie Przedmieście 32, 00-927 Warsaw</wicri:regionArea><wicri:noRegion>00-927 Warsaw</wicri:noRegion></affiliation></author></titleStmt><publicationStmt><idno type="wicri:source">PMC</idno><idno type="pmid">24793446</idno><idno type="pmc">4185099</idno><idno type="url">http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4185099</idno><idno type="RBID">PMC:4185099</idno><idno type="doi">10.1007/s13353-014-0216-y</idno><date when="2014">2014</date><idno type="wicri:Area/Pmc/Corpus">000008</idno><idno type="wicri:explorRef" wicri:stream="Pmc" wicri:step="Corpus" wicri:corpus="PMC">000008</idno><idno type="wicri:Area/Pmc/Curation">000008</idno><idno type="wicri:explorRef" wicri:stream="Pmc" wicri:step="Curation">000008</idno><idno type="wicri:Area/Pmc/Checkpoint">000012</idno><idno type="wicri:explorRef" wicri:stream="Pmc" wicri:step="Checkpoint">000012</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en" level="a" type="main">Microsatellite multiplex assay for the analysis of Atlantic sturgeon populations</title><author><name sortKey="Panagiotopoulou, H" sort="Panagiotopoulou, H" uniqKey="Panagiotopoulou H" first="H." last="Panagiotopoulou">H. Panagiotopoulou</name><affiliation wicri:level="1"><nlm:aff id="Aff1">Institute of Biochemistry and Biophysics, Polish Academy of Sciences, Pawińskiego 5a, 02-106 Warsaw, Poland</nlm:aff><country xml:lang="fr">Pologne</country><wicri:regionArea>Institute of Biochemistry and Biophysics, Polish Academy of Sciences, Pawińskiego 5a, 02-106 Warsaw</wicri:regionArea><wicri:noRegion>02-106 Warsaw</wicri:noRegion></affiliation><affiliation wicri:level="1"><nlm:aff id="Aff5">Institute of Genetics and Biotechnology, University of Warsaw, Pawińskiego 5a, 02-106 Warsaw, Poland</nlm:aff><country xml:lang="fr">Pologne</country><wicri:regionArea>Institute of Genetics and Biotechnology, University of Warsaw, Pawińskiego 5a, 02-106 Warsaw</wicri:regionArea><wicri:noRegion>02-106 Warsaw</wicri:noRegion></affiliation></author><author><name sortKey="Popovic, D" sort="Popovic, D" uniqKey="Popovic D" first="D." last="Popovic">D. Popovic</name><affiliation wicri:level="1"><nlm:aff id="Aff2">Centre of New Technologies (CeNT), University of Warsaw, ul. Żwirki i Wigury 93, 02-089 Warsaw, Poland</nlm:aff><country xml:lang="fr">Pologne</country><wicri:regionArea>Centre of New Technologies (CeNT), University of Warsaw, ul. Żwirki i Wigury 93, 02-089 Warsaw</wicri:regionArea><wicri:noRegion>02-089 Warsaw</wicri:noRegion></affiliation></author><author><name sortKey="Zalewska, K" sort="Zalewska, K" uniqKey="Zalewska K" first="K." last="Zalewska">K. Zalewska</name><affiliation wicri:level="1"><nlm:aff id="Aff3">Faculty of Biology, Institute of Genetics and Biotechnology, University of Warsaw, Pawińskiego 5a, 02-106 Warsaw, Poland</nlm:aff><country xml:lang="fr">Pologne</country><wicri:regionArea>Faculty of Biology, Institute of Genetics and Biotechnology, University of Warsaw, Pawińskiego 5a, 02-106 Warsaw</wicri:regionArea><wicri:noRegion>02-106 Warsaw</wicri:noRegion></affiliation></author><author><name sortKey="Weglenski, P" sort="Weglenski, P" uniqKey="Weglenski P" first="P." last="Weglenski">P. Weglenski</name><affiliation wicri:level="1"><nlm:aff id="Aff1">Institute of Biochemistry and Biophysics, Polish Academy of Sciences, Pawińskiego 5a, 02-106 Warsaw, Poland</nlm:aff><country xml:lang="fr">Pologne</country><wicri:regionArea>Institute of Biochemistry and Biophysics, Polish Academy of Sciences, Pawińskiego 5a, 02-106 Warsaw</wicri:regionArea><wicri:noRegion>02-106 Warsaw</wicri:noRegion></affiliation><affiliation wicri:level="1"><nlm:aff id="Aff2">Centre of New Technologies (CeNT), University of Warsaw, ul. Żwirki i Wigury 93, 02-089 Warsaw, Poland</nlm:aff><country xml:lang="fr">Pologne</country><wicri:regionArea>Centre of New Technologies (CeNT), University of Warsaw, ul. Żwirki i Wigury 93, 02-089 Warsaw</wicri:regionArea><wicri:noRegion>02-089 Warsaw</wicri:noRegion></affiliation></author><author><name sortKey="Stankovic, A" sort="Stankovic, A" uniqKey="Stankovic A" first="A." last="Stankovic">A. Stankovic</name><affiliation wicri:level="1"><nlm:aff id="Aff1">Institute of Biochemistry and Biophysics, Polish Academy of Sciences, Pawińskiego 5a, 02-106 Warsaw, Poland</nlm:aff><country xml:lang="fr">Pologne</country><wicri:regionArea>Institute of Biochemistry and Biophysics, Polish Academy of Sciences, Pawińskiego 5a, 02-106 Warsaw</wicri:regionArea><wicri:noRegion>02-106 Warsaw</wicri:noRegion></affiliation><affiliation wicri:level="1"><nlm:aff id="Aff3">Faculty of Biology, Institute of Genetics and Biotechnology, University of Warsaw, Pawińskiego 5a, 02-106 Warsaw, Poland</nlm:aff><country xml:lang="fr">Pologne</country><wicri:regionArea>Faculty of Biology, Institute of Genetics and Biotechnology, University of Warsaw, Pawińskiego 5a, 02-106 Warsaw</wicri:regionArea><wicri:noRegion>02-106 Warsaw</wicri:noRegion></affiliation><affiliation wicri:level="1"><nlm:aff id="Aff4">The Antiquity of Southeastern Europe Research Center, University of Warsaw, Krakowskie Przedmieście 32, 00-927 Warsaw, Poland</nlm:aff><country xml:lang="fr">Pologne</country><wicri:regionArea>The Antiquity of Southeastern Europe Research Center, University of Warsaw, Krakowskie Przedmieście 32, 00-927 Warsaw</wicri:regionArea><wicri:noRegion>00-927 Warsaw</wicri:noRegion></affiliation></author></analytic><series><title level="j">Journal of Applied Genetics</title><idno type="ISSN">1234-1983</idno><idno type="eISSN">2190-3883</idno><imprint><date when="2014">2014</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en"><p>We have developed a multiplex assay covering 16 microsatellite loci, amplified in four polymerase chain reaction (PCR) assays, and loaded on the ABI DNA Analyzer in two separate panels. The assay was tested on 603 individuals originating from wild populations and hatchery stocks of Atlantic sturgeon. The assay was also tested on 12 individuals of European sturgeon and appeared to be almost equally useful. The multiplex assay designed in this study can be successfully applied in studies requiring high genetic resolution, such as relatedness analysis, selective breeding programs, and stock identification of Atlantic sturgeon.</p><sec><title>Electronic supplementary material</title><p>The online version of this article (doi:10.1007/s13353-014-0216-y) contains supplementary material, which is available to authorized users.</p></sec></div></front><back><div1 type="bibliography"><listBibl><biblStruct><analytic><author><name sortKey="Blouin, Ms" uniqKey="Blouin M">MS Blouin</name></author><author><name sortKey="Parsons, M" uniqKey="Parsons M">M Parsons</name></author><author><name sortKey="Lacaille, V" uniqKey="Lacaille V">V Lacaille</name></author><author><name sortKey="Lotz, S" uniqKey="Lotz S">S Lotz</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Brookfield, Jfy" uniqKey="Brookfield J">JFY Brookfield</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Chassaing, O" uniqKey="Chassaing O">O Chassaing</name></author><author><name sortKey="Desse Berset, N" uniqKey="Desse Berset N">N Desse-Berset</name></author><author><name sortKey="Duffraisse, M" uniqKey="Duffraisse M">M Duffraisse</name></author><author><name sortKey="Piques, G" uniqKey="Piques G">G Piquès</name></author><author><name sortKey="H Nni, C" uniqKey="H Nni C">C Hänni</name></author><author><name sortKey="Berrebi, P" uniqKey="Berrebi P">P Berrebi</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Chassaing, O" uniqKey="Chassaing O">O Chassaing</name></author><author><name sortKey="H Nni, C" uniqKey="H Nni C">C Hänni</name></author><author><name sortKey="Berrebi, P" uniqKey="Berrebi P">P Berrebi</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Chassaing, O" uniqKey="Chassaing O">O Chassaing</name></author><author><name sortKey="Desse Berset, N" uniqKey="Desse Berset N">N Desse-Berset</name></author><author><name sortKey="Duffraisse, M" uniqKey="Duffraisse M">M Duffraisse</name></author><author><name sortKey="Hughes, S" uniqKey="Hughes S">S Hughes</name></author><author><name sortKey="H Nni, C" uniqKey="H Nni C">C Hänni</name></author><author><name sortKey="Berrebi, P" uniqKey="Berrebi P">P Berrebi</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Ciofi, C" uniqKey="Ciofi C">C Ciofi</name></author><author><name sortKey="Tzika, Ac" uniqKey="Tzika A">AC Tzika</name></author><author><name sortKey="Natali, C" uniqKey="Natali C">C Natali</name></author><author><name sortKey="Watts, Pc" uniqKey="Watts P">PC Watts</name></author><author><name sortKey="Sulandari, S" uniqKey="Sulandari S">S Sulandari</name></author><author><name sortKey="Zein, Msa" uniqKey="Zein M">MSA Zein</name></author><author><name sortKey="Milinkovitch, Mc" uniqKey="Milinkovitch M">MC Milinkovitch</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Desalle, R" uniqKey="Desalle R">R DeSalle</name></author><author><name sortKey="Amato, G" uniqKey="Amato G">G Amato</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Excoffier, L" uniqKey="Excoffier L">L Excoffier</name></author><author><name sortKey="Lischer, Hel" uniqKey="Lischer H">HEL Lischer</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Gessner, J" uniqKey="Gessner J">J Gessner</name></author><author><name sortKey="Tautenhahn, M" uniqKey="Tautenhahn M">M Tautenhahn</name></author><author><name sortKey="Spratte, S" uniqKey="Spratte S">S Spratte</name></author><author><name sortKey="Arndt, G M" uniqKey="Arndt G">G-M Arndt</name></author><author><name sortKey="Von Nordheim, H" uniqKey="Von Nordheim H">H von Nordheim</name></author></analytic></biblStruct><biblStruct></biblStruct><biblStruct><analytic><author><name sortKey="Henderson Arzapalo, A" uniqKey="Henderson Arzapalo A">A Henderson-Arzapalo</name></author><author><name sortKey="King, Tl" uniqKey="King T">TL King</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Kalendar, R" uniqKey="Kalendar R">R Kalendar</name></author><author><name sortKey="Lee, D" uniqKey="Lee D">D Lee</name></author><author><name sortKey="Schulman, Ah" uniqKey="Schulman A">AH Schulman</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Kalinowski, St" uniqKey="Kalinowski S">ST Kalinowski</name></author><author><name sortKey="Taper, Ml" uniqKey="Taper M">ML Taper</name></author><author><name sortKey="Marshall, Tc" uniqKey="Marshall T">TC Marshall</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="King, Tl" uniqKey="King T">TL King</name></author><author><name sortKey="Lubinski, Ba" uniqKey="Lubinski B">BA Lubinski</name></author><author><name sortKey="Spidle, Ap" uniqKey="Spidle A">AP Spidle</name></author></analytic></biblStruct><biblStruct></biblStruct><biblStruct><analytic><author><name sortKey="Kolman, R" uniqKey="Kolman R">R Kolman</name></author><author><name sortKey="Kapusta, A" uniqKey="Kapusta A">A Kapusta</name></author><author><name sortKey="Duda, A" uniqKey="Duda A">A Duda</name></author><author><name sortKey="Wiszniewski, G" uniqKey="Wiszniewski G">G Wiszniewski</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Lassalle, G" uniqKey="Lassalle G">G Lassalle</name></author><author><name sortKey="Beguer, M" uniqKey="Beguer M">M Béguer</name></author><author><name sortKey="Rochard, E" uniqKey="Rochard E">E Rochard</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Ludwig, A" uniqKey="Ludwig A">A Ludwig</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Ludwig, An" uniqKey="Ludwig A">AN Ludwig</name></author><author><name sortKey="Jenneckens, I" uniqKey="Jenneckens I">I Jenneckens</name></author><author><name sortKey="Debus, L" uniqKey="Debus L">L Debus</name></author><author><name sortKey="Ludwig, A" uniqKey="Ludwig A">A Ludwig</name></author><author><name sortKey="Becker, J" uniqKey="Becker J">J Becker</name></author><author><name sortKey="Kirschbaum, F" uniqKey="Kirschbaum F">F Kirschbaum</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Ludwig, A" uniqKey="Ludwig A">A Ludwig</name></author><author><name sortKey="Debus, L" uniqKey="Debus L">L Debus</name></author><author><name sortKey="Lieckfeldt, D" uniqKey="Lieckfeldt D">D Lieckfeldt</name></author><author><name sortKey="Wirgin, I" uniqKey="Wirgin I">I Wirgin</name></author><author><name sortKey="Benecke, N" uniqKey="Benecke N">N Benecke</name></author><author><name sortKey="Jenneckens, I" uniqKey="Jenneckens I">I Jenneckens</name></author><author><name sortKey="Williot, P" uniqKey="Williot P">P Williot</name></author><author><name sortKey="Waldman, Jr" uniqKey="Waldman J">JR Waldman</name></author><author><name sortKey="Pitra, C" uniqKey="Pitra C">C Pitra</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Ludwig, A" uniqKey="Ludwig A">A Ludwig</name></author><author><name sortKey="Arndt, U" uniqKey="Arndt U">U Arndt</name></author><author><name sortKey="Lippold, S" uniqKey="Lippold S">S Lippold</name></author><author><name sortKey="Benecke, N" uniqKey="Benecke N">N Benecke</name></author><author><name sortKey="Debus, L" uniqKey="Debus L">L Debus</name></author><author><name sortKey="King, Tl" uniqKey="King T">TL King</name></author><author><name sortKey="Matsumura, S" uniqKey="Matsumura S">S Matsumura</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Ludwig, A" uniqKey="Ludwig A">A Ludwig</name></author><author><name sortKey="Makowiecki, D" uniqKey="Makowiecki D">D Makowiecki</name></author><author><name sortKey="Benecke, N" uniqKey="Benecke N">N Benecke</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="May, B" uniqKey="May B">B May</name></author><author><name sortKey="Krueger, Cc" uniqKey="Krueger C">CC Krueger</name></author><author><name sortKey="Kincaid, Kl" uniqKey="Kincaid K">KL Kincaid</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Moyer, Gr" uniqKey="Moyer G">GR Moyer</name></author><author><name sortKey="Sweka, Ja" uniqKey="Sweka J">JA Sweka</name></author><author><name sortKey="Peterson, Dl" uniqKey="Peterson D">DL Peterson</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Norris, At" uniqKey="Norris A">AT Norris</name></author><author><name sortKey="Bradley, Dg" uniqKey="Bradley D">DG Bradley</name></author><author><name sortKey="Cunningham, Ep" uniqKey="Cunningham E">EP Cunningham</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Olafsson, K" uniqKey="Olafsson K">K Olafsson</name></author><author><name sortKey="Hjorleifsdottir, S" uniqKey="Hjorleifsdottir S">S Hjorleifsdottir</name></author><author><name sortKey="Pampoulie, C" uniqKey="Pampoulie C">C Pampoulie</name></author><author><name sortKey="Hreggvidsson, Go" uniqKey="Hreggvidsson G">GO Hreggvidsson</name></author><author><name sortKey="Gudjonsson, S" uniqKey="Gudjonsson S">S Gudjonsson</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Paaver, T" uniqKey="Paaver T">T Paaver</name></author></analytic></biblStruct><biblStruct></biblStruct><biblStruct><analytic><author><name sortKey="Pikitch, Ek" uniqKey="Pikitch E">EK Pikitch</name></author><author><name sortKey="Doukakis, P" uniqKey="Doukakis P">P Doukakis</name></author><author><name sortKey="Lauck, L" uniqKey="Lauck L">L Lauck</name></author><author><name sortKey="Chakrabarty, P" uniqKey="Chakrabarty P">P Chakrabarty</name></author><author><name sortKey="Erickson, Dl" uniqKey="Erickson D">DL Erickson</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Raymond, M" uniqKey="Raymond M">M Raymond</name></author><author><name sortKey="Rousset, F" uniqKey="Rousset F">F Rousset</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Saarinen, Ev" uniqKey="Saarinen E">EV Saarinen</name></author><author><name sortKey="Flowers, Jh" uniqKey="Flowers J">JH Flowers</name></author><author><name sortKey="Pine, We" uniqKey="Pine W">WE Pine</name></author><author><name sortKey="Parauka, F" uniqKey="Parauka F">F Parauka</name></author><author><name sortKey="Austin, Jd" uniqKey="Austin J">JD Austin</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Schlotterer, C" uniqKey="Schlotterer C">C Schlötterer</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Stankovic, A" uniqKey="Stankovic A">A Stankovic</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Tiedemann, R" uniqKey="Tiedemann R">R Tiedemann</name></author><author><name sortKey="Schneider, A" uniqKey="Schneider A">A Schneider</name></author><author><name sortKey="Williot, P" uniqKey="Williot P">P Williot</name></author><author><name sortKey="Kirschbaum, F" uniqKey="Kirschbaum F">F Kirschbaum</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Van Oosterhout, C" uniqKey="Van Oosterhout C">C Van Oosterhout</name></author><author><name sortKey="Hutchinson, Wf" uniqKey="Hutchinson W">WF Hutchinson</name></author><author><name sortKey="Wills, Dpm" uniqKey="Wills D">DPM Wills</name></author><author><name sortKey="Shipley, P" uniqKey="Shipley P">P Shipley</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Vecsei, P" uniqKey="Vecsei P">P Vecsei</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Waldman, Jr" uniqKey="Waldman J">JR Waldman</name></author><author><name sortKey="Wirgin, Ii" uniqKey="Wirgin I">II Wirgin</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Waldman, Jr" uniqKey="Waldman J">JR Waldman</name></author><author><name sortKey="King, T" uniqKey="King T">T King</name></author><author><name sortKey="Savoy, T" uniqKey="Savoy T">T Savoy</name></author><author><name sortKey="Maceda, L" uniqKey="Maceda L">L Maceda</name></author><author><name sortKey="Grunwald, C" uniqKey="Grunwald C">C Grunwald</name></author><author><name sortKey="Wirgin, I" uniqKey="Wirgin I">I Wirgin</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Williot, P" uniqKey="Williot P">P Williot</name></author><author><name sortKey="Rouault, T" uniqKey="Rouault T">T Rouault</name></author><author><name sortKey="Pelard, M" uniqKey="Pelard M">M Pelard</name></author><author><name sortKey="Mercier, D" uniqKey="Mercier D">D Mercier</name></author><author><name sortKey="Lepage, M" uniqKey="Lepage M">M Lepage</name></author><author><name sortKey="Davail Cluisset, B" uniqKey="Davail Cluisset B">B Davail-Cluisset</name></author><author><name sortKey="Kirschbaum, F" uniqKey="Kirschbaum F">F Kirschbaum</name></author><author><name sortKey="Ludwig, A" uniqKey="Ludwig A">A Ludwig</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Wirgin, I" uniqKey="Wirgin I">I Wirgin</name></author><author><name sortKey="Waldman, Jr" uniqKey="Waldman J">JR Waldman</name></author><author><name sortKey="Stabile, J" uniqKey="Stabile J">J Stabile</name></author><author><name sortKey="Lubinski, B" uniqKey="Lubinski B">B Lubinski</name></author><author><name sortKey="King, T" uniqKey="King T">T King</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Wirgin, I" uniqKey="Wirgin I">I Wirgin</name></author><author><name sortKey="Maceda, L" uniqKey="Maceda L">L Maceda</name></author><author><name sortKey="Waldman, Jr" uniqKey="Waldman J">JR Waldman</name></author><author><name sortKey="Wehrell, S" uniqKey="Wehrell S">S Wehrell</name></author><author><name sortKey="Dadswell, D" uniqKey="Dadswell D">D Dadswell</name></author><author><name sortKey="King, T" uniqKey="King T">T King</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Zhu, Zy" uniqKey="Zhu Z">ZY Zhu</name></author><author><name sortKey="Wang, Cm" uniqKey="Wang C">CM Wang</name></author><author><name sortKey="Lo, Lc" uniqKey="Lo L">LC Lo</name></author><author><name sortKey="Lin, G" uniqKey="Lin G">G Lin</name></author><author><name sortKey="Feng, F" uniqKey="Feng F">F Feng</name></author><author><name sortKey="Tan, J" uniqKey="Tan J">J Tan</name></author><author><name sortKey="Chou, R" uniqKey="Chou R">R Chou</name></author><author><name sortKey="Lim, Hs" uniqKey="Lim H">HS Lim</name></author><author><name sortKey="Orban, L" uniqKey="Orban L">L Orban</name></author><author><name sortKey="Yue, Gh" uniqKey="Yue G">GH Yue</name></author></analytic></biblStruct></listBibl></div1></back></TEI><pmc article-type="brief-report"><pmc-dir>properties open_access</pmc-dir>
<front><journal-meta><journal-id journal-id-type="nlm-ta">J Appl Genet</journal-id><journal-id journal-id-type="iso-abbrev">J. Appl. Genet</journal-id><journal-title-group><journal-title>Journal of Applied Genetics</journal-title></journal-title-group><issn pub-type="ppub">1234-1983</issn><issn pub-type="epub">2190-3883</issn><publisher><publisher-name>Springer Berlin Heidelberg</publisher-name><publisher-loc>Berlin/Heidelberg</publisher-loc></publisher></journal-meta><article-meta><article-id pub-id-type="pmid">24793446</article-id><article-id pub-id-type="pmc">4185099</article-id><article-id pub-id-type="publisher-id">216</article-id><article-id pub-id-type="doi">10.1007/s13353-014-0216-y</article-id><article-categories><subj-group subj-group-type="heading"><subject>Animal Genetics • Short Communication</subject></subj-group></article-categories><title-group><article-title>Microsatellite multiplex assay for the analysis of Atlantic sturgeon populations</article-title></title-group><contrib-group><contrib contrib-type="author" corresp="yes"><name><surname>Panagiotopoulou</surname><given-names>H.</given-names></name><address><phone>+48-22-5923233</phone><fax>+48-22-6584176</fax><email>hpana@wp.pl</email></address><xref ref-type="aff" rid="Aff1"></xref><xref ref-type="aff" rid="Aff5"></xref></contrib><contrib contrib-type="author"><name><surname>Popovic</surname><given-names>D.</given-names></name><xref ref-type="aff" rid="Aff2"></xref></contrib><contrib contrib-type="author"><name><surname>Zalewska</surname><given-names>K.</given-names></name><xref ref-type="aff" rid="Aff3"></xref></contrib><contrib contrib-type="author"><name><surname>Weglenski</surname><given-names>P.</given-names></name><xref ref-type="aff" rid="Aff1"></xref><xref ref-type="aff" rid="Aff2"></xref></contrib><contrib contrib-type="author"><name><surname>Stankovic</surname><given-names>A.</given-names></name><xref ref-type="aff" rid="Aff1"></xref><xref ref-type="aff" rid="Aff3"></xref><xref ref-type="aff" rid="Aff4"></xref></contrib><aff id="Aff1"><label></label>Institute of Biochemistry and Biophysics, Polish Academy of Sciences, Pawińskiego 5a, 02-106 Warsaw, Poland</aff><aff id="Aff2"><label></label>Centre of New Technologies (CeNT), University of Warsaw, ul. Żwirki i Wigury 93, 02-089 Warsaw, Poland</aff><aff id="Aff3"><label></label>Faculty of Biology, Institute of Genetics and Biotechnology, University of Warsaw, Pawińskiego 5a, 02-106 Warsaw, Poland</aff><aff id="Aff4"><label></label>The Antiquity of Southeastern Europe Research Center, University of Warsaw, Krakowskie Przedmieście 32, 00-927 Warsaw, Poland</aff><aff id="Aff5"><label></label>Institute of Genetics and Biotechnology, University of Warsaw, Pawińskiego 5a, 02-106 Warsaw, Poland</aff></contrib-group><pub-date pub-type="epub"><day>4</day><month>5</month><year>2014</year></pub-date><pub-date pub-type="pmc-release"><day>4</day><month>5</month><year>2014</year></pub-date><pub-date pub-type="ppub"><year>2014</year></pub-date><volume>55</volume><issue>4</issue><fpage>505</fpage><lpage>510</lpage><history><date date-type="received"><day>2</day><month>9</month><year>2013</year></date><date date-type="accepted"><day>7</day><month>4</month><year>2014</year></date></history><permissions><copyright-statement>© The Author(s) 2014</copyright-statement><license license-type="OpenAccess"><license-p><bold>Open Access</bold> This article is distributed under the terms of the Creative Commons Attribution License which permits any use, distribution, and reproduction in any medium, provided the original author(s) and the source are credited.</license-p></license></permissions><abstract id="Abs1"><p>We have developed a multiplex assay covering 16 microsatellite loci, amplified in four polymerase chain reaction (PCR) assays, and loaded on the ABI DNA Analyzer in two separate panels. The assay was tested on 603 individuals originating from wild populations and hatchery stocks of Atlantic sturgeon. The assay was also tested on 12 individuals of European sturgeon and appeared to be almost equally useful. The multiplex assay designed in this study can be successfully applied in studies requiring high genetic resolution, such as relatedness analysis, selective breeding programs, and stock identification of Atlantic sturgeon.</p><sec><title>Electronic supplementary material</title><p>The online version of this article (doi:10.1007/s13353-014-0216-y) contains supplementary material, which is available to authorized users.</p></sec></abstract><kwd-group xml:lang="en"><title>Keywords</title><kwd>Microsatellite loci</kwd><kwd>Multiplex PCR</kwd><kwd>Atlantic sturgeon</kwd><kwd>European sturgeon</kwd></kwd-group><custom-meta-group><custom-meta><meta-name>issue-copyright-statement</meta-name><meta-value>© Institute of Plant Genetics, Polish Academy of Sciences, Poznan 2014</meta-value></custom-meta></custom-meta-group></article-meta></front></pmc><affiliations><list><country><li>Pologne</li></country></list><tree><country name="Pologne"><noRegion><name sortKey="Panagiotopoulou, H" sort="Panagiotopoulou, H" uniqKey="Panagiotopoulou H" first="H." last="Panagiotopoulou">H. Panagiotopoulou</name></noRegion><name sortKey="Panagiotopoulou, H" sort="Panagiotopoulou, H" uniqKey="Panagiotopoulou H" first="H." last="Panagiotopoulou">H. Panagiotopoulou</name><name sortKey="Popovic, D" sort="Popovic, D" uniqKey="Popovic D" first="D." last="Popovic">D. Popovic</name><name sortKey="Stankovic, A" sort="Stankovic, A" uniqKey="Stankovic A" first="A." last="Stankovic">A. Stankovic</name><name sortKey="Stankovic, A" sort="Stankovic, A" uniqKey="Stankovic A" first="A." last="Stankovic">A. Stankovic</name><name sortKey="Stankovic, A" sort="Stankovic, A" uniqKey="Stankovic A" first="A." last="Stankovic">A. Stankovic</name><name sortKey="Weglenski, P" sort="Weglenski, P" uniqKey="Weglenski P" first="P." last="Weglenski">P. Weglenski</name><name sortKey="Weglenski, P" sort="Weglenski, P" uniqKey="Weglenski P" first="P." last="Weglenski">P. Weglenski</name><name sortKey="Zalewska, K" sort="Zalewska, K" uniqKey="Zalewska K" first="K." last="Zalewska">K. Zalewska</name></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
EXPLOR_STEP=$WICRI_ROOT/Wicri/Eau/explor/EsturgeonV1/Data/Pmc/Checkpoint
HfdSelect -h $EXPLOR_STEP/biblio.hfd -nk 000012 | SxmlIndent | more
Ou
HfdSelect -h $EXPLOR_AREA/Data/Pmc/Checkpoint/biblio.hfd -nk 000012 | SxmlIndent | more
Pour mettre un lien sur cette page dans le réseau Wicri
{{Explor lien
|wiki= Wicri/Eau
|area= EsturgeonV1
|flux= Pmc
|étape= Checkpoint
|type= RBID
|clé= PMC:4185099
|texte= Microsatellite multiplex assay for the analysis of Atlantic sturgeon populations
}}
Pour générer des pages wiki
HfdIndexSelect -h $EXPLOR_AREA/Data/Pmc/Checkpoint/RBID.i -Sk "pubmed:24793446" \
| HfdSelect -Kh $EXPLOR_AREA/Data/Pmc/Checkpoint/biblio.hfd \
| NlmPubMed2Wicri -a EsturgeonV1
| This area was generated with Dilib version V0.6.27. |  |