Effects of pre-incubation of eggs in fresh water and varying sperm concentration on fertilization rate in sterlet sturgeon, Acipenser ruthenus.
Identifieur interne : 000136 ( Main/Merge ); précédent : 000135; suivant : 000137Effects of pre-incubation of eggs in fresh water and varying sperm concentration on fertilization rate in sterlet sturgeon, Acipenser ruthenus.
Auteurs : Mohammad Abdul Momin Siddique [République tchèque] ; Ian Anthony Ernest Butts [Danemark] ; Martin Psenicka [République tchèque] ; Otomar Linhart [République tchèque]Source :
- Animal reproduction science [ 1873-2232 ] ; 2015.
English descriptors
- KwdEn :
- MESH :
- chemical : Water.
- physiology : Fertilization, Fishes, Ovum, Spermatozoa.
- veterinary : Fertilization in Vitro.
- Animals, Male, Sperm Count, Time Factors.
Abstract
Standardization of fertilization protocols for sterlet Acipenser ruthenus is crucial for improving reproductive techniques and for conservation purposes. Our objectives were to determine the number of sperm (tested 430,000:1, 43,000:1, 4300:1, 430:1 sperm to egg) required to fertilize eggs and explore how pre-incubation of eggs in freshwater for 0min, 0.5min, 1min, and 10min interacts with different sperm ratios. Fertilization success ranged from 29.7% at 430:1 to 84.2% at 430,000:1. Pre-incubation time had no effect on fertilization success at 430,000:1 and 43,000:1 sperm to egg ratios, while it was significant at the 4300:1 and 430:1 ratios. The use of adequate experimental suboptimal sperm to egg ratio revealed a positive effect of pre-incubation time, such that at the 430:1 ratio, 0.5min pre-incubation increased the fertilization rate than 10min. At 0min pre-incubation the proportion of fertilized eggs increased at the 430,000:1 ratio, while at 1min fertilization increased at the 4300:1 ratio. At the 10min pre-incubation time, fertilization increased at the 43,000:1 ratio. Moreover, at the 0.5min pre-incubation time, the 43,000:1 ratio increased the fertilization rate than the 430:1 ratio. Generally, for 430:1 ratio, the fertilization rate is lower than in control. Transmission electron microscopy showed that pre-incubation of eggs in water for <10min does not trigger a cortical reaction or the formation of a perivitelline space. Results suggest that with a low sperm to egg ratio 0.5 to 1min pre-incubation of eggs in freshwater prior to fertilization can enhance fertilization rate of sterlet.
DOI: 10.1016/j.anireprosci.2015.06.008
PubMed: 26119142
Links toward previous steps (curation, corpus...)
- to stream PubMed, to step Corpus: 000120
- to stream PubMed, to step Curation: 000120
- to stream PubMed, to step Checkpoint: 000120
- to stream Ncbi, to step Merge: 000638
- to stream Ncbi, to step Curation: 000638
- to stream Ncbi, to step Checkpoint: 000638
Links to Exploration step
pubmed:26119142Le document en format XML
<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">Effects of pre-incubation of eggs in fresh water and varying sperm concentration on fertilization rate in sterlet sturgeon, Acipenser ruthenus.</title><author><name sortKey="Siddique, Mohammad Abdul Momin" sort="Siddique, Mohammad Abdul Momin" uniqKey="Siddique M" first="Mohammad Abdul Momin" last="Siddique">Mohammad Abdul Momin Siddique</name><affiliation wicri:level="1"><nlm:affiliation>University of South Bohemia in Ceske Budejovice, Faculty of Fisheries and Protection of Waters, South Bohemian Research Center of Aquaculture and Biodiversity of Hydrocenoses, Research Institute of Fish Culture and Hydrobiology, Zátiší 728/II, 389 25 Vodňany, Czech Republic. Electronic address: siddique@frov.jcu.cz.</nlm:affiliation><country xml:lang="fr">République tchèque</country><wicri:regionArea>University of South Bohemia in Ceske Budejovice, Faculty of Fisheries and Protection of Waters, South Bohemian Research Center of Aquaculture and Biodiversity of Hydrocenoses, Research Institute of Fish Culture and Hydrobiology, Zátiší 728/II, 389 25 Vodňany</wicri:regionArea><wicri:noRegion>389 25 Vodňany</wicri:noRegion></affiliation></author><author><name sortKey="Butts, Ian Anthony Ernest" sort="Butts, Ian Anthony Ernest" uniqKey="Butts I" first="Ian Anthony Ernest" last="Butts">Ian Anthony Ernest Butts</name><affiliation wicri:level="1"><nlm:affiliation>DTU Aqua-National Institute of Aquatic Resources, Section for Marine Ecology and Oceanography, Technical University of Denmark, Charlottenlund Slot, Jægersborg Allé 1, 2920 Charlottenlund, Denmark.</nlm:affiliation><country xml:lang="fr">Danemark</country><wicri:regionArea>DTU Aqua-National Institute of Aquatic Resources, Section for Marine Ecology and Oceanography, Technical University of Denmark, Charlottenlund Slot, Jægersborg Allé 1, 2920 Charlottenlund</wicri:regionArea><wicri:noRegion>2920 Charlottenlund</wicri:noRegion></affiliation></author><author><name sortKey="Psenicka, Martin" sort="Psenicka, Martin" uniqKey="Psenicka M" first="Martin" last="Psenicka">Martin Psenicka</name><affiliation wicri:level="1"><nlm:affiliation>University of South Bohemia in Ceske Budejovice, Faculty of Fisheries and Protection of Waters, South Bohemian Research Center of Aquaculture and Biodiversity of Hydrocenoses, Research Institute of Fish Culture and Hydrobiology, Zátiší 728/II, 389 25 Vodňany, Czech Republic.</nlm:affiliation><country xml:lang="fr">République tchèque</country><wicri:regionArea>University of South Bohemia in Ceske Budejovice, Faculty of Fisheries and Protection of Waters, South Bohemian Research Center of Aquaculture and Biodiversity of Hydrocenoses, Research Institute of Fish Culture and Hydrobiology, Zátiší 728/II, 389 25 Vodňany</wicri:regionArea><wicri:noRegion>389 25 Vodňany</wicri:noRegion></affiliation></author><author><name sortKey="Linhart, Otomar" sort="Linhart, Otomar" uniqKey="Linhart O" first="Otomar" last="Linhart">Otomar Linhart</name><affiliation wicri:level="1"><nlm:affiliation>University of South Bohemia in Ceske Budejovice, Faculty of Fisheries and Protection of Waters, South Bohemian Research Center of Aquaculture and Biodiversity of Hydrocenoses, Research Institute of Fish Culture and Hydrobiology, Zátiší 728/II, 389 25 Vodňany, Czech Republic. Electronic address: linhart@frov.jcu.cz.</nlm:affiliation><country xml:lang="fr">République tchèque</country><wicri:regionArea>University of South Bohemia in Ceske Budejovice, Faculty of Fisheries and Protection of Waters, South Bohemian Research Center of Aquaculture and Biodiversity of Hydrocenoses, Research Institute of Fish Culture and Hydrobiology, Zátiší 728/II, 389 25 Vodňany</wicri:regionArea><wicri:noRegion>389 25 Vodňany</wicri:noRegion></affiliation></author></titleStmt><publicationStmt><idno type="wicri:source">PubMed</idno><date when="2015">2015</date><idno type="RBID">pubmed:26119142</idno><idno type="pmid">26119142</idno><idno type="doi">10.1016/j.anireprosci.2015.06.008</idno><idno type="wicri:Area/PubMed/Corpus">000120</idno><idno type="wicri:explorRef" wicri:stream="PubMed" wicri:step="Corpus" wicri:corpus="PubMed">000120</idno><idno type="wicri:Area/PubMed/Curation">000120</idno><idno type="wicri:explorRef" wicri:stream="PubMed" wicri:step="Curation">000120</idno><idno type="wicri:Area/PubMed/Checkpoint">000120</idno><idno type="wicri:explorRef" wicri:stream="Checkpoint" wicri:step="PubMed">000120</idno><idno type="wicri:Area/Ncbi/Merge">000638</idno><idno type="wicri:Area/Ncbi/Curation">000638</idno><idno type="wicri:Area/Ncbi/Checkpoint">000638</idno><idno type="wicri:Area/Main/Merge">000136</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en">Effects of pre-incubation of eggs in fresh water and varying sperm concentration on fertilization rate in sterlet sturgeon, Acipenser ruthenus.</title><author><name sortKey="Siddique, Mohammad Abdul Momin" sort="Siddique, Mohammad Abdul Momin" uniqKey="Siddique M" first="Mohammad Abdul Momin" last="Siddique">Mohammad Abdul Momin Siddique</name><affiliation wicri:level="1"><nlm:affiliation>University of South Bohemia in Ceske Budejovice, Faculty of Fisheries and Protection of Waters, South Bohemian Research Center of Aquaculture and Biodiversity of Hydrocenoses, Research Institute of Fish Culture and Hydrobiology, Zátiší 728/II, 389 25 Vodňany, Czech Republic. Electronic address: siddique@frov.jcu.cz.</nlm:affiliation><country xml:lang="fr">République tchèque</country><wicri:regionArea>University of South Bohemia in Ceske Budejovice, Faculty of Fisheries and Protection of Waters, South Bohemian Research Center of Aquaculture and Biodiversity of Hydrocenoses, Research Institute of Fish Culture and Hydrobiology, Zátiší 728/II, 389 25 Vodňany</wicri:regionArea><wicri:noRegion>389 25 Vodňany</wicri:noRegion></affiliation></author><author><name sortKey="Butts, Ian Anthony Ernest" sort="Butts, Ian Anthony Ernest" uniqKey="Butts I" first="Ian Anthony Ernest" last="Butts">Ian Anthony Ernest Butts</name><affiliation wicri:level="1"><nlm:affiliation>DTU Aqua-National Institute of Aquatic Resources, Section for Marine Ecology and Oceanography, Technical University of Denmark, Charlottenlund Slot, Jægersborg Allé 1, 2920 Charlottenlund, Denmark.</nlm:affiliation><country xml:lang="fr">Danemark</country><wicri:regionArea>DTU Aqua-National Institute of Aquatic Resources, Section for Marine Ecology and Oceanography, Technical University of Denmark, Charlottenlund Slot, Jægersborg Allé 1, 2920 Charlottenlund</wicri:regionArea><wicri:noRegion>2920 Charlottenlund</wicri:noRegion></affiliation></author><author><name sortKey="Psenicka, Martin" sort="Psenicka, Martin" uniqKey="Psenicka M" first="Martin" last="Psenicka">Martin Psenicka</name><affiliation wicri:level="1"><nlm:affiliation>University of South Bohemia in Ceske Budejovice, Faculty of Fisheries and Protection of Waters, South Bohemian Research Center of Aquaculture and Biodiversity of Hydrocenoses, Research Institute of Fish Culture and Hydrobiology, Zátiší 728/II, 389 25 Vodňany, Czech Republic.</nlm:affiliation><country xml:lang="fr">République tchèque</country><wicri:regionArea>University of South Bohemia in Ceske Budejovice, Faculty of Fisheries and Protection of Waters, South Bohemian Research Center of Aquaculture and Biodiversity of Hydrocenoses, Research Institute of Fish Culture and Hydrobiology, Zátiší 728/II, 389 25 Vodňany</wicri:regionArea><wicri:noRegion>389 25 Vodňany</wicri:noRegion></affiliation></author><author><name sortKey="Linhart, Otomar" sort="Linhart, Otomar" uniqKey="Linhart O" first="Otomar" last="Linhart">Otomar Linhart</name><affiliation wicri:level="1"><nlm:affiliation>University of South Bohemia in Ceske Budejovice, Faculty of Fisheries and Protection of Waters, South Bohemian Research Center of Aquaculture and Biodiversity of Hydrocenoses, Research Institute of Fish Culture and Hydrobiology, Zátiší 728/II, 389 25 Vodňany, Czech Republic. Electronic address: linhart@frov.jcu.cz.</nlm:affiliation><country xml:lang="fr">République tchèque</country><wicri:regionArea>University of South Bohemia in Ceske Budejovice, Faculty of Fisheries and Protection of Waters, South Bohemian Research Center of Aquaculture and Biodiversity of Hydrocenoses, Research Institute of Fish Culture and Hydrobiology, Zátiší 728/II, 389 25 Vodňany</wicri:regionArea><wicri:noRegion>389 25 Vodňany</wicri:noRegion></affiliation></author></analytic><series><title level="j">Animal reproduction science</title><idno type="eISSN">1873-2232</idno><imprint><date when="2015" type="published">2015</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Animals</term><term>Fertilization (physiology)</term><term>Fertilization in Vitro (veterinary)</term><term>Fishes (physiology)</term><term>Male</term><term>Ovum (physiology)</term><term>Sperm Count</term><term>Spermatozoa (physiology)</term><term>Time Factors</term><term>Water</term></keywords><keywords scheme="MESH" type="chemical" xml:lang="en"><term>Water</term></keywords><keywords scheme="MESH" qualifier="physiology" xml:lang="en"><term>Fertilization</term><term>Fishes</term><term>Ovum</term><term>Spermatozoa</term></keywords><keywords scheme="MESH" qualifier="veterinary" xml:lang="en"><term>Fertilization in Vitro</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Animals</term><term>Male</term><term>Sperm Count</term><term>Time Factors</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Standardization of fertilization protocols for sterlet Acipenser ruthenus is crucial for improving reproductive techniques and for conservation purposes. Our objectives were to determine the number of sperm (tested 430,000:1, 43,000:1, 4300:1, 430:1 sperm to egg) required to fertilize eggs and explore how pre-incubation of eggs in freshwater for 0min, 0.5min, 1min, and 10min interacts with different sperm ratios. Fertilization success ranged from 29.7% at 430:1 to 84.2% at 430,000:1. Pre-incubation time had no effect on fertilization success at 430,000:1 and 43,000:1 sperm to egg ratios, while it was significant at the 4300:1 and 430:1 ratios. The use of adequate experimental suboptimal sperm to egg ratio revealed a positive effect of pre-incubation time, such that at the 430:1 ratio, 0.5min pre-incubation increased the fertilization rate than 10min. At 0min pre-incubation the proportion of fertilized eggs increased at the 430,000:1 ratio, while at 1min fertilization increased at the 4300:1 ratio. At the 10min pre-incubation time, fertilization increased at the 43,000:1 ratio. Moreover, at the 0.5min pre-incubation time, the 43,000:1 ratio increased the fertilization rate than the 430:1 ratio. Generally, for 430:1 ratio, the fertilization rate is lower than in control. Transmission electron microscopy showed that pre-incubation of eggs in water for <10min does not trigger a cortical reaction or the formation of a perivitelline space. Results suggest that with a low sperm to egg ratio 0.5 to 1min pre-incubation of eggs in freshwater prior to fertilization can enhance fertilization rate of sterlet.</div></front></TEI></record>Pour manipuler ce document sous Unix (Dilib)
EXPLOR_STEP=$WICRI_ROOT/Wicri/Eau/explor/EsturgeonV1/Data/Main/Merge
HfdSelect -h $EXPLOR_STEP/biblio.hfd -nk 000136 | SxmlIndent | more
Ou
HfdSelect -h $EXPLOR_AREA/Data/Main/Merge/biblio.hfd -nk 000136 | SxmlIndent | more
Pour mettre un lien sur cette page dans le réseau Wicri
{{Explor lien
|wiki= Wicri/Eau
|area= EsturgeonV1
|flux= Main
|étape= Merge
|type= RBID
|clé= pubmed:26119142
|texte= Effects of pre-incubation of eggs in fresh water and varying sperm concentration on fertilization rate in sterlet sturgeon, Acipenser ruthenus.
}}
Pour générer des pages wiki
HfdIndexSelect -h $EXPLOR_AREA/Data/Main/Merge/RBID.i -Sk "pubmed:26119142" \
| HfdSelect -Kh $EXPLOR_AREA/Data/Main/Merge/biblio.hfd \
| NlmPubMed2Wicri -a EsturgeonV1
| This area was generated with Dilib version V0.6.27. |  |