Effects of pre-incubation of eggs in fresh water and varying sperm concentration on fertilization rate in sterlet sturgeon, Acipenser ruthenus.
Identifieur interne : 000120 ( PubMed/Corpus ); précédent : 000119; suivant : 000121Effects of pre-incubation of eggs in fresh water and varying sperm concentration on fertilization rate in sterlet sturgeon, Acipenser ruthenus.
Auteurs : Mohammad Abdul Momin Siddique ; Ian Anthony Ernest Butts ; Martin Psenicka ; Otomar LinhartSource :
- Animal reproduction science [ 1873-2232 ] ; 2015.
English descriptors
- KwdEn :
- MESH :
- chemical : Water.
- physiology : Fertilization, Fishes, Ovum, Spermatozoa.
- veterinary : Fertilization in Vitro.
- Animals, Male, Sperm Count, Time Factors.
Abstract
Standardization of fertilization protocols for sterlet Acipenser ruthenus is crucial for improving reproductive techniques and for conservation purposes. Our objectives were to determine the number of sperm (tested 430,000:1, 43,000:1, 4300:1, 430:1 sperm to egg) required to fertilize eggs and explore how pre-incubation of eggs in freshwater for 0min, 0.5min, 1min, and 10min interacts with different sperm ratios. Fertilization success ranged from 29.7% at 430:1 to 84.2% at 430,000:1. Pre-incubation time had no effect on fertilization success at 430,000:1 and 43,000:1 sperm to egg ratios, while it was significant at the 4300:1 and 430:1 ratios. The use of adequate experimental suboptimal sperm to egg ratio revealed a positive effect of pre-incubation time, such that at the 430:1 ratio, 0.5min pre-incubation increased the fertilization rate than 10min. At 0min pre-incubation the proportion of fertilized eggs increased at the 430,000:1 ratio, while at 1min fertilization increased at the 4300:1 ratio. At the 10min pre-incubation time, fertilization increased at the 43,000:1 ratio. Moreover, at the 0.5min pre-incubation time, the 43,000:1 ratio increased the fertilization rate than the 430:1 ratio. Generally, for 430:1 ratio, the fertilization rate is lower than in control. Transmission electron microscopy showed that pre-incubation of eggs in water for <10min does not trigger a cortical reaction or the formation of a perivitelline space. Results suggest that with a low sperm to egg ratio 0.5 to 1min pre-incubation of eggs in freshwater prior to fertilization can enhance fertilization rate of sterlet.
DOI: 10.1016/j.anireprosci.2015.06.008
PubMed: 26119142
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pubmed:26119142Le document en format XML
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Electronic address: siddique@frov.jcu.cz.</nlm:affiliation></affiliation></author><author><name sortKey="Butts, Ian Anthony Ernest" sort="Butts, Ian Anthony Ernest" uniqKey="Butts I" first="Ian Anthony Ernest" last="Butts">Ian Anthony Ernest Butts</name><affiliation><nlm:affiliation>DTU Aqua-National Institute of Aquatic Resources, Section for Marine Ecology and Oceanography, Technical University of Denmark, Charlottenlund Slot, Jægersborg Allé 1, 2920 Charlottenlund, Denmark.</nlm:affiliation></affiliation></author><author><name sortKey="Psenicka, Martin" sort="Psenicka, Martin" uniqKey="Psenicka M" first="Martin" last="Psenicka">Martin Psenicka</name><affiliation><nlm:affiliation>University of South Bohemia in Ceske Budejovice, Faculty of Fisheries and Protection of Waters, South Bohemian Research Center of Aquaculture and Biodiversity of Hydrocenoses, Research Institute of Fish Culture and Hydrobiology, Zátiší 728/II, 389 25 Vodňany, Czech Republic.</nlm:affiliation></affiliation></author><author><name sortKey="Linhart, Otomar" sort="Linhart, Otomar" uniqKey="Linhart O" first="Otomar" last="Linhart">Otomar Linhart</name><affiliation><nlm:affiliation>University of South Bohemia in Ceske Budejovice, Faculty of Fisheries and Protection of Waters, South Bohemian Research Center of Aquaculture and Biodiversity of Hydrocenoses, Research Institute of Fish Culture and Hydrobiology, Zátiší 728/II, 389 25 Vodňany, Czech Republic. 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Electronic address: siddique@frov.jcu.cz.</nlm:affiliation></affiliation></author><author><name sortKey="Butts, Ian Anthony Ernest" sort="Butts, Ian Anthony Ernest" uniqKey="Butts I" first="Ian Anthony Ernest" last="Butts">Ian Anthony Ernest Butts</name><affiliation><nlm:affiliation>DTU Aqua-National Institute of Aquatic Resources, Section for Marine Ecology and Oceanography, Technical University of Denmark, Charlottenlund Slot, Jægersborg Allé 1, 2920 Charlottenlund, Denmark.</nlm:affiliation></affiliation></author><author><name sortKey="Psenicka, Martin" sort="Psenicka, Martin" uniqKey="Psenicka M" first="Martin" last="Psenicka">Martin Psenicka</name><affiliation><nlm:affiliation>University of South Bohemia in Ceske Budejovice, Faculty of Fisheries and Protection of Waters, South Bohemian Research Center of Aquaculture and Biodiversity of Hydrocenoses, Research Institute of Fish Culture and Hydrobiology, Zátiší 728/II, 389 25 Vodňany, Czech Republic.</nlm:affiliation></affiliation></author><author><name sortKey="Linhart, Otomar" sort="Linhart, Otomar" uniqKey="Linhart O" first="Otomar" last="Linhart">Otomar Linhart</name><affiliation><nlm:affiliation>University of South Bohemia in Ceske Budejovice, Faculty of Fisheries and Protection of Waters, South Bohemian Research Center of Aquaculture and Biodiversity of Hydrocenoses, Research Institute of Fish Culture and Hydrobiology, Zátiší 728/II, 389 25 Vodňany, Czech Republic. Electronic address: linhart@frov.jcu.cz.</nlm:affiliation></affiliation></author></analytic><series><title level="j">Animal reproduction science</title><idno type="eISSN">1873-2232</idno><imprint><date when="2015" type="published">2015</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Animals</term><term>Fertilization (physiology)</term><term>Fertilization in Vitro (veterinary)</term><term>Fishes (physiology)</term><term>Male</term><term>Ovum (physiology)</term><term>Sperm Count</term><term>Spermatozoa (physiology)</term><term>Time Factors</term><term>Water</term></keywords><keywords scheme="MESH" type="chemical" xml:lang="en"><term>Water</term></keywords><keywords scheme="MESH" qualifier="physiology" xml:lang="en"><term>Fertilization</term><term>Fishes</term><term>Ovum</term><term>Spermatozoa</term></keywords><keywords scheme="MESH" qualifier="veterinary" xml:lang="en"><term>Fertilization in Vitro</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Animals</term><term>Male</term><term>Sperm Count</term><term>Time Factors</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Standardization of fertilization protocols for sterlet Acipenser ruthenus is crucial for improving reproductive techniques and for conservation purposes. Our objectives were to determine the number of sperm (tested 430,000:1, 43,000:1, 4300:1, 430:1 sperm to egg) required to fertilize eggs and explore how pre-incubation of eggs in freshwater for 0min, 0.5min, 1min, and 10min interacts with different sperm ratios. Fertilization success ranged from 29.7% at 430:1 to 84.2% at 430,000:1. Pre-incubation time had no effect on fertilization success at 430,000:1 and 43,000:1 sperm to egg ratios, while it was significant at the 4300:1 and 430:1 ratios. The use of adequate experimental suboptimal sperm to egg ratio revealed a positive effect of pre-incubation time, such that at the 430:1 ratio, 0.5min pre-incubation increased the fertilization rate than 10min. At 0min pre-incubation the proportion of fertilized eggs increased at the 430,000:1 ratio, while at 1min fertilization increased at the 4300:1 ratio. At the 10min pre-incubation time, fertilization increased at the 43,000:1 ratio. Moreover, at the 0.5min pre-incubation time, the 43,000:1 ratio increased the fertilization rate than the 430:1 ratio. Generally, for 430:1 ratio, the fertilization rate is lower than in control. Transmission electron microscopy showed that pre-incubation of eggs in water for <10min does not trigger a cortical reaction or the formation of a perivitelline space. Results suggest that with a low sperm to egg ratio 0.5 to 1min pre-incubation of eggs in freshwater prior to fertilization can enhance fertilization rate of sterlet.</div></front></TEI><pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">26119142</PMID><DateCreated><Year>2015</Year><Month>07</Month><Day>21</Day></DateCreated><DateCompleted><Year>2016</Year><Month>04</Month><Day>25</Day></DateCompleted><DateRevised><Year>2015</Year><Month>07</Month><Day>21</Day></DateRevised><Article PubModel="Print-Electronic"><Journal><ISSN IssnType="Electronic">1873-2232</ISSN><JournalIssue CitedMedium="Internet"><Volume>159</Volume><PubDate><Year>2015</Year><Month>Aug</Month></PubDate></JournalIssue><Title>Animal reproduction science</Title><ISOAbbreviation>Anim. Reprod. Sci.</ISOAbbreviation></Journal><ArticleTitle>Effects of pre-incubation of eggs in fresh water and varying sperm concentration on fertilization rate in sterlet sturgeon, Acipenser ruthenus.</ArticleTitle><Pagination><MedlinePgn>141-7</MedlinePgn></Pagination><ELocationID EIdType="doi" ValidYN="Y">10.1016/j.anireprosci.2015.06.008</ELocationID><ELocationID EIdType="pii" ValidYN="Y">S0378-4320(15)00139-6</ELocationID><Abstract><AbstractText>Standardization of fertilization protocols for sterlet Acipenser ruthenus is crucial for improving reproductive techniques and for conservation purposes. Our objectives were to determine the number of sperm (tested 430,000:1, 43,000:1, 4300:1, 430:1 sperm to egg) required to fertilize eggs and explore how pre-incubation of eggs in freshwater for 0min, 0.5min, 1min, and 10min interacts with different sperm ratios. Fertilization success ranged from 29.7% at 430:1 to 84.2% at 430,000:1. Pre-incubation time had no effect on fertilization success at 430,000:1 and 43,000:1 sperm to egg ratios, while it was significant at the 4300:1 and 430:1 ratios. The use of adequate experimental suboptimal sperm to egg ratio revealed a positive effect of pre-incubation time, such that at the 430:1 ratio, 0.5min pre-incubation increased the fertilization rate than 10min. At 0min pre-incubation the proportion of fertilized eggs increased at the 430,000:1 ratio, while at 1min fertilization increased at the 4300:1 ratio. At the 10min pre-incubation time, fertilization increased at the 43,000:1 ratio. Moreover, at the 0.5min pre-incubation time, the 43,000:1 ratio increased the fertilization rate than the 430:1 ratio. Generally, for 430:1 ratio, the fertilization rate is lower than in control. Transmission electron microscopy showed that pre-incubation of eggs in water for <10min does not trigger a cortical reaction or the formation of a perivitelline space. Results suggest that with a low sperm to egg ratio 0.5 to 1min pre-incubation of eggs in freshwater prior to fertilization can enhance fertilization rate of sterlet.</AbstractText><CopyrightInformation>Copyright © 2015 Elsevier B.V. All rights reserved.</CopyrightInformation></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Siddique</LastName><ForeName>Mohammad Abdul Momin</ForeName><Initials>MA</Initials><AffiliationInfo><Affiliation>University of South Bohemia in Ceske Budejovice, Faculty of Fisheries and Protection of Waters, South Bohemian Research Center of Aquaculture and Biodiversity of Hydrocenoses, Research Institute of Fish Culture and Hydrobiology, Zátiší 728/II, 389 25 Vodňany, Czech Republic. Electronic address: siddique@frov.jcu.cz.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Butts</LastName><ForeName>Ian Anthony Ernest</ForeName><Initials>IA</Initials><AffiliationInfo><Affiliation>DTU Aqua-National Institute of Aquatic Resources, Section for Marine Ecology and Oceanography, Technical University of Denmark, Charlottenlund Slot, Jægersborg Allé 1, 2920 Charlottenlund, Denmark.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Psenicka</LastName><ForeName>Martin</ForeName><Initials>M</Initials><AffiliationInfo><Affiliation>University of South Bohemia in Ceske Budejovice, Faculty of Fisheries and Protection of Waters, South Bohemian Research Center of Aquaculture and Biodiversity of Hydrocenoses, Research Institute of Fish Culture and Hydrobiology, Zátiší 728/II, 389 25 Vodňany, Czech Republic.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Linhart</LastName><ForeName>Otomar</ForeName><Initials>O</Initials><AffiliationInfo><Affiliation>University of South Bohemia in Ceske Budejovice, Faculty of Fisheries and Protection of Waters, South Bohemian Research Center of Aquaculture and Biodiversity of Hydrocenoses, Research Institute of Fish Culture and Hydrobiology, Zátiší 728/II, 389 25 Vodňany, Czech Republic. Electronic address: linhart@frov.jcu.cz.</Affiliation></AffiliationInfo></Author></AuthorList><Language>eng</Language><PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType><PublicationType UI="D013485">Research Support, Non-U.S. Gov't</PublicationType></PublicationTypeList><ArticleDate DateType="Electronic"><Year>2015</Year><Month>06</Month><Day>12</Day></ArticleDate></Article><MedlineJournalInfo><Country>Netherlands</Country><MedlineTA>Anim Reprod Sci</MedlineTA><NlmUniqueID>7807205</NlmUniqueID><ISSNLinking>0378-4320</ISSNLinking></MedlineJournalInfo><ChemicalList><Chemical><RegistryNumber>059QF0KO0R</RegistryNumber><NameOfSubstance UI="D014867">Water</NameOfSubstance></Chemical></ChemicalList><CitationSubset>IM</CitationSubset><MeshHeadingList><MeshHeading><DescriptorName UI="D000818" MajorTopicYN="N">Animals</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D005306" MajorTopicYN="N">Fertilization</DescriptorName><QualifierName UI="Q000502" MajorTopicYN="Y">physiology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D005307" MajorTopicYN="N">Fertilization in Vitro</DescriptorName><QualifierName UI="Q000662" MajorTopicYN="N">veterinary</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D005399" MajorTopicYN="N">Fishes</DescriptorName><QualifierName UI="Q000502" MajorTopicYN="Y">physiology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D008297" MajorTopicYN="N">Male</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D010063" MajorTopicYN="N">Ovum</DescriptorName><QualifierName UI="Q000502" MajorTopicYN="Y">physiology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D013076" MajorTopicYN="N">Sperm Count</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D013094" MajorTopicYN="N">Spermatozoa</DescriptorName><QualifierName UI="Q000502" MajorTopicYN="Y">physiology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D013997" MajorTopicYN="N">Time Factors</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D014867" MajorTopicYN="N">Water</DescriptorName></MeshHeading></MeshHeadingList><KeywordList Owner="NOTNLM"><Keyword MajorTopicYN="N">Artificial reproduction</Keyword><Keyword MajorTopicYN="N">Effects of pre-incubation</Keyword><Keyword MajorTopicYN="N">Fertilization success</Keyword><Keyword MajorTopicYN="N">Sperm to egg ratio</Keyword></KeywordList></MedlineCitation><PubmedData><History><PubMedPubDate PubStatus="received"><Year>2015</Year><Month>02</Month><Day>05</Day></PubMedPubDate><PubMedPubDate PubStatus="revised"><Year>2015</Year><Month>06</Month><Day>02</Day></PubMedPubDate><PubMedPubDate PubStatus="accepted"><Year>2015</Year><Month>06</Month><Day>08</Day></PubMedPubDate><PubMedPubDate PubStatus="entrez"><Year>2015</Year><Month>6</Month><Day>30</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="pubmed"><Year>2015</Year><Month>6</Month><Day>30</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="medline"><Year>2016</Year><Month>4</Month><Day>26</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate></History><PublicationStatus>ppublish</PublicationStatus><ArticleIdList><ArticleId IdType="pubmed">26119142</ArticleId><ArticleId IdType="pii">S0378-4320(15)00139-6</ArticleId><ArticleId IdType="doi">10.1016/j.anireprosci.2015.06.008</ArticleId></ArticleIdList></PubmedData></pubmed></record>Pour manipuler ce document sous Unix (Dilib)
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