Short-term storage of sterlet Acipenser ruthenus testicular cells at -80 °C.
Identifieur interne : 000034 ( Main/Exploration ); précédent : 000033; suivant : 000035Short-term storage of sterlet Acipenser ruthenus testicular cells at -80 °C.
Auteurs : Amin Golpour [République tchèque] ; Mohammad Abdul Momin Siddique [République tchèque] ; Marek Rodina [République tchèque] ; Martin Pšeni Ka [République tchèque]Source :
- Cryobiology [ 1090-2392 ] ; 2016.
English descriptors
- KwdEn :
- MESH :
- chemical , pharmacology : Cryoprotective Agents, Ethylene Glycol.
- cytology : Testis.
- methods : Cryopreservation, Organ Preservation.
- Animals, Cell Survival, Endangered Species, Fishes, Male, Temperature.
Abstract
The conservation of sturgeons is of critical importance, and optimization of long-term storage is crucial to cell survival. This study aimed to examine the viability rates of several variations of sturgeon testicular cells storage at -80 °C for purpose of a short-term storage in a deep freezer or shipment on dried ice. Testes extracted from three immature fish were cut into small pieces, immersed in a cryomedium composed of phosphate buffered saline with 0.5% bovine serum albumin, 50 mM glucose, and 1.5 M ethylene glycol as a cryoprotectant, chilled from 10 to -80 °C at a cooling rate of 1 °C per min, and stored under varying conditions. Our results revealed a significant effect of storage conditions on the number of living and dead cells (p > 0.05). Samples that were stored for 7 days at -80 °C showed a considerable decline in terms of cell viability compared to samples stored for 2 days storage at -80 °C or in LN. This result indicated that testicular cells can be stored at -80 °C and/or on dry ice, for 2 days with minimum loss of viability.
DOI: 10.1016/j.cryobiol.2016.03.002
PubMed: 26964775
Affiliations:
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Le document en format XML
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This study aimed to examine the viability rates of several variations of sturgeon testicular cells storage at -80 °C for purpose of a short-term storage in a deep freezer or shipment on dried ice. Testes extracted from three immature fish were cut into small pieces, immersed in a cryomedium composed of phosphate buffered saline with 0.5% bovine serum albumin, 50 mM glucose, and 1.5 M ethylene glycol as a cryoprotectant, chilled from 10 to -80 °C at a cooling rate of 1 °C per min, and stored under varying conditions. Our results revealed a significant effect of storage conditions on the number of living and dead cells (p > 0.05). Samples that were stored for 7 days at -80 °C showed a considerable decline in terms of cell viability compared to samples stored for 2 days storage at -80 °C or in LN. This result indicated that testicular cells can be stored at -80 °C and/or on dry ice, for 2 days with minimum loss of viability.</div></front></TEI><affiliations><list><country><li>République tchèque</li></country></list><tree><country name="République tchèque"><noRegion><name sortKey="Golpour, Amin" sort="Golpour, Amin" uniqKey="Golpour A" first="Amin" last="Golpour">Amin Golpour</name></noRegion><name sortKey="Pseni Ka, Martin" sort="Pseni Ka, Martin" uniqKey="Pseni Ka M" first="Martin" last="Pšeni Ka">Martin Pšeni Ka</name><name sortKey="Rodina, Marek" sort="Rodina, Marek" uniqKey="Rodina M" first="Marek" last="Rodina">Marek Rodina</name><name sortKey="Siddique, Mohammad Abdul Momin" sort="Siddique, Mohammad Abdul Momin" uniqKey="Siddique M" first="Mohammad Abdul Momin" last="Siddique">Mohammad Abdul Momin Siddique</name></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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