Partial deglycosylation of alpha subunit modifies sturgeon gonadotropin function.
Identifieur interne : 001510 ( Main/Exploration ); précédent : 001509; suivant : 001511Partial deglycosylation of alpha subunit modifies sturgeon gonadotropin function.
Auteurs : H. Zenkevics [Lettonie] ; V. Vose ; I. Vosekalne ; A. B CenaSource :
- Acta biochimica Polonica [ 0001-527X ] ; 2000.
English descriptors
- KwdEn :
- MESH :
- chemical , chemistry : Gonadotropins, Pituitary, Recombinant Proteins.
- physiology : Fishes, Gonadotropins, Pituitary.
- Animals, Circular Dichroism, Dimerization, Glycosylation, Protein Structure, Secondary, Protein Subunits.
Abstract
Chemical deglycosylation (dg) of sturgeon Acipenser gueldenstaedti Br. (alphaGTH) resulted in the loss of 83% of its initial carbohydrate content. It altered also recombinant dg alphaGTH + betaGTH dimer molecule, reducing its immunoreactivity by 30%, and fully blocking the hormonal function. CD spectroscopy showed that deglycosylation led to changes in the secondary structure of dg alphaGTH and in the alpha-beta recombinant. The sugar moiety of sturgeon alphaGTH is suggested to play an important role in maintaining the biological function of the hormone dimer molecule.
PubMed: 11310980
Affiliations:
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Le document en format XML
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Zenkevics</name><affiliation wicri:level="1"><nlm:affiliation>Laboratory of Biochemistry, Institute of Biology, Latvian University, Salaspils. zenkevics@email.lubi.edu.lv</nlm:affiliation><country wicri:rule="url">Lettonie</country></affiliation></author><author><name sortKey="Vose, V" sort="Vose, V" uniqKey="Vose V" first="V" last="Vose">V. Vose</name></author><author><name sortKey="Vosekalne, I" sort="Vosekalne, I" uniqKey="Vosekalne I" first="I" last="Vosekalne">I. Vosekalne</name></author><author><name sortKey="B Cena, A" sort="B Cena, A" uniqKey="B Cena A" first="A" last="B Cena">A. B Cena</name></author></analytic><series><title level="j">Acta biochimica Polonica</title><idno type="ISSN">0001-527X</idno><imprint><date when="2000" type="published">2000</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Animals</term><term>Circular Dichroism</term><term>Dimerization</term><term>Fishes (physiology)</term><term>Glycosylation</term><term>Gonadotropins, Pituitary (chemistry)</term><term>Gonadotropins, Pituitary (physiology)</term><term>Protein Structure, Secondary</term><term>Protein Subunits</term><term>Recombinant Proteins (chemistry)</term></keywords><keywords scheme="MESH" type="chemical" qualifier="chemistry" xml:lang="en"><term>Gonadotropins, Pituitary</term><term>Recombinant Proteins</term></keywords><keywords scheme="MESH" qualifier="physiology" xml:lang="en"><term>Fishes</term><term>Gonadotropins, Pituitary</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Animals</term><term>Circular Dichroism</term><term>Dimerization</term><term>Glycosylation</term><term>Protein Structure, Secondary</term><term>Protein Subunits</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Chemical deglycosylation (dg) of sturgeon Acipenser gueldenstaedti Br. (alphaGTH) resulted in the loss of 83% of its initial carbohydrate content. It altered also recombinant dg alphaGTH + betaGTH dimer molecule, reducing its immunoreactivity by 30%, and fully blocking the hormonal function. CD spectroscopy showed that deglycosylation led to changes in the secondary structure of dg alphaGTH and in the alpha-beta recombinant. The sugar moiety of sturgeon alphaGTH is suggested to play an important role in maintaining the biological function of the hormone dimer molecule.</div></front></TEI><affiliations><list><country><li>Lettonie</li></country></list><tree><noCountry><name sortKey="B Cena, A" sort="B Cena, A" uniqKey="B Cena A" first="A" last="B Cena">A. B Cena</name><name sortKey="Vose, V" sort="Vose, V" uniqKey="Vose V" first="V" last="Vose">V. Vose</name><name sortKey="Vosekalne, I" sort="Vosekalne, I" uniqKey="Vosekalne I" first="I" last="Vosekalne">I. Vosekalne</name></noCountry><country name="Lettonie"><noRegion><name sortKey="Zenkevics, H" sort="Zenkevics, H" uniqKey="Zenkevics H" first="H" last="Zenkevics">H. Zenkevics</name></noRegion></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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