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Mammalian gonadotropin-releasing hormone (GnRH) identified by primary structure in Russian sturgeon, Acipenser gueldenstaedti.

Identifieur interne : 000649 ( PubMed/Checkpoint ); précédent : 000648; suivant : 000650

Mammalian gonadotropin-releasing hormone (GnRH) identified by primary structure in Russian sturgeon, Acipenser gueldenstaedti.

Auteurs : D W Lescheid [Canada] ; J F Powell ; W H Fischer ; M. Park ; A. Craig ; O. Bukovskaya ; I A Barannikova ; N M Sherwood

Source :

Regulatory peptides [ 0167-0115 ] ; 1995.

RBID : pubmed:7761629

English descriptors

KwdEn :
- Amino Acid Sequence, Animals, Brain Chemistry, Chromatography, High Pressure Liquid, Female, Fishes, Gonadotropin-Releasing Hormone (analysis), Gonadotropin-Releasing Hormone (chemistry), Male, Mass Spectrometry, Molecular Sequence Data, Sequence Analysis.
MESH :
- chemical , analysis : Gonadotropin-Releasing Hormone.
- chemical , chemistry : Gonadotropin-Releasing Hormone.
- Amino Acid Sequence, Animals, Brain Chemistry, Chromatography, High Pressure Liquid, Female, Fishes, Male, Mass Spectrometry, Molecular Sequence Data, Sequence Analysis.

Abstract

The mammalian form of gonadotropin-releasing hormone (GnRH) was purified from the brains of Russian sturgeon, Acipenser gueldenstaedti, using reversed-phase high pressure liquid chromatography (HPLC). The total concentration of mGnRH within these fish was 5.4 ng/brain. Small amounts of immunoreactive chicken GnRH-II like molecules were also detected but at insufficient quantities for purification. The primary structure of mGnRH was determined using automated Edman degradation. Because sequence data could not be obtained until after digestion by bovine pyroglutamyl amino-peptidase, it was determined that the amino-terminal residue was modified. Furthermore, mass spectrometric data and co-elution with synthetic mGnRH on HPLC confirmed that the carboxy-terminal residue was amidated. The amino acid sequence of sturgeon GnRH is pGlu-His-Trp-Ser-Tyr-Gly-Leu-Arg-Pro-Gly-NH2.

PubMed: 7761629

Affiliations:

Canada

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pubmed:7761629

Le document en format XML

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<author><name sortKey="Lescheid, D W" sort="Lescheid, D W" uniqKey="Lescheid D" first="D W" last="Lescheid">D W Lescheid</name>
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<author><name sortKey="Powell, J F" sort="Powell, J F" uniqKey="Powell J" first="J F" last="Powell">J F Powell</name>
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<author><name sortKey="Fischer, W H" sort="Fischer, W H" uniqKey="Fischer W" first="W H" last="Fischer">W H Fischer</name>
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<author><name sortKey="Park, M" sort="Park, M" uniqKey="Park M" first="M" last="Park">M. Park</name>
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<author><name sortKey="Craig, A" sort="Craig, A" uniqKey="Craig A" first="A" last="Craig">A. Craig</name>
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<author><name sortKey="Bukovskaya, O" sort="Bukovskaya, O" uniqKey="Bukovskaya O" first="O" last="Bukovskaya">O. Bukovskaya</name>
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<author><name sortKey="Barannikova, I A" sort="Barannikova, I A" uniqKey="Barannikova I" first="I A" last="Barannikova">I A Barannikova</name>
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<term>Female</term>
<term>Fishes</term>
<term>Gonadotropin-Releasing Hormone (analysis)</term>
<term>Gonadotropin-Releasing Hormone (chemistry)</term>
<term>Male</term>
<term>Mass Spectrometry</term>
<term>Molecular Sequence Data</term>
<term>Sequence Analysis</term>
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<front><div type="abstract" xml:lang="en">The mammalian form of gonadotropin-releasing hormone (GnRH) was purified from the brains of Russian sturgeon, Acipenser gueldenstaedti, using reversed-phase high pressure liquid chromatography (HPLC). The total concentration of mGnRH within these fish was 5.4 ng/brain. Small amounts of immunoreactive chicken GnRH-II like molecules were also detected but at insufficient quantities for purification. The primary structure of mGnRH was determined using automated Edman degradation. Because sequence data could not be obtained until after digestion by bovine pyroglutamyl amino-peptidase, it was determined that the amino-terminal residue was modified. Furthermore, mass spectrometric data and co-elution with synthetic mGnRH on HPLC confirmed that the carboxy-terminal residue was amidated. The amino acid sequence of sturgeon GnRH is pGlu-His-Trp-Ser-Tyr-Gly-Leu-Arg-Pro-Gly-NH2.</div>
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<Title>Regulatory peptides</Title>
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<Abstract><AbstractText>The mammalian form of gonadotropin-releasing hormone (GnRH) was purified from the brains of Russian sturgeon, Acipenser gueldenstaedti, using reversed-phase high pressure liquid chromatography (HPLC). The total concentration of mGnRH within these fish was 5.4 ng/brain. Small amounts of immunoreactive chicken GnRH-II like molecules were also detected but at insufficient quantities for purification. The primary structure of mGnRH was determined using automated Edman degradation. Because sequence data could not be obtained until after digestion by bovine pyroglutamyl amino-peptidase, it was determined that the amino-terminal residue was modified. Furthermore, mass spectrometric data and co-elution with synthetic mGnRH on HPLC confirmed that the carboxy-terminal residue was amidated. The amino acid sequence of sturgeon GnRH is pGlu-His-Trp-Ser-Tyr-Gly-Leu-Arg-Pro-Gly-NH2.</AbstractText>
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Mammalian gonadotropin-releasing hormone (GnRH) identified by primary structure in Russian sturgeon, Acipenser gueldenstaedti.

Mammalian gonadotropin-releasing hormone (GnRH) identified by primary structure in Russian sturgeon, Acipenser gueldenstaedti.

Source :

English descriptors

Abstract

Links toward previous steps (curation, corpus...)

Links to Exploration step

Le document en format XML

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Pour générer des pages wiki