Artificial gynogenesis in Cynoglossus semilaevis with homologous sperm and its verification using microsatellite markers
Identifieur interne : 000857 ( Main/Exploration ); précédent : 000856; suivant : 000858Artificial gynogenesis in Cynoglossus semilaevis with homologous sperm and its verification using microsatellite markers
Auteurs : Xiang-Shan Ji [République populaire de Chine] ; Yong-Sheng Tian [République populaire de Chine] ; Jing-Feng Yang [République populaire de Chine] ; Peng-Fei Wu [République populaire de Chine] ; Yun-Liang Jiang [République populaire de Chine] ; Song-Lin Chen [République populaire de Chine]Source :
- Aquaculture Research [ 1355-557X ] ; 2010-05.
English descriptors
Abstract
Effective methods for induction of gynogenetic diploids in Cynoglossus semilaevis are needed to initiate monosex culture. An effective protocol to induce half‐smooth tongue sole gynogenesis using homologous sperm was developed in this study. A UV dose of 50 mJ cm−2 was found to be the most effective for genetic inactivation of tongue sole sperm. Treatment optima for cold shocks were 5 °C for 20–23 min at 5 min after fertilization and the hatching rate of gynogenetic diploids was 10.0%. Microsatellite analysis at locus Csou 6 revealed that there was no genetic contribution from the paternal genome in 24 progenies of a meiotic gynogenetic family. Polymerase chain reaction demonstrated that only four individuals of 24 meiotic gynogenetic diploids produced the female‐specific band of about 205 bp. The female/male ratio of gynogenetic diploids was significantly different from the theoretical ratio of 1:1. It is possible that there are some recessive lethal genes in W chromosome.
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DOI: 10.1111/j.1365-2109.2009.02373.x
Affiliations:
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induction of gynogenetic diploids in Cynoglossus semilaevis are needed to initiate monosex culture. An effective protocol to induce half‐smooth tongue sole gynogenesis using homologous sperm was developed in this study. A UV dose of 50 mJ cm−2 was found to be the most effective for genetic inactivation of tongue sole sperm. Treatment optima for cold shocks were 5 °C for 20–23 min at 5 min after fertilization and the hatching rate of gynogenetic diploids was 10.0%. Microsatellite analysis at locus Csou 6 revealed that there was no genetic contribution from the paternal genome in 24 progenies of a meiotic gynogenetic family. Polymerase chain reaction demonstrated that only four individuals of 24 meiotic gynogenetic diploids produced the female‐specific band of about 205 bp. The female/male ratio of gynogenetic diploids was significantly different from the theoretical ratio of 1:1. It is possible that there are some recessive lethal genes in W chromosome.</div></front></TEI><affiliations><list><country><li>République populaire de Chine</li></country></list><tree><country name="République populaire de Chine"><noRegion><name sortKey="Ji, Xiang Han" sort="Ji, Xiang Han" uniqKey="Ji X" first="Xiang-Shan" last="Ji">Xiang-Shan Ji</name></noRegion><name sortKey="Chen, Song In" sort="Chen, Song In" uniqKey="Chen S" first="Song-Lin" last="Chen">Song-Lin Chen</name><name sortKey="Ji, Xiang Han" sort="Ji, Xiang Han" uniqKey="Ji X" first="Xiang-Shan" last="Ji">Xiang-Shan Ji</name><name sortKey="Jiang, Yun Iang" sort="Jiang, Yun Iang" uniqKey="Jiang Y" first="Yun-Liang" last="Jiang">Yun-Liang Jiang</name><name sortKey="Tian, Yong Heng" sort="Tian, Yong Heng" uniqKey="Tian Y" first="Yong-Sheng" last="Tian">Yong-Sheng Tian</name><name sortKey="Wu, Peng Ei" sort="Wu, Peng Ei" uniqKey="Wu P" first="Peng-Fei" last="Wu">Peng-Fei Wu</name><name sortKey="Yang, Jing Eng" sort="Yang, Jing Eng" uniqKey="Yang J" first="Jing-Feng" last="Yang">Jing-Feng Yang</name></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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