Artificial gynogenesis in Cynoglossus semilaevis with homologous sperm and its verification using microsatellite markers
Identifieur interne : 000859 ( Istex/Corpus ); précédent : 000858; suivant : 000860Artificial gynogenesis in Cynoglossus semilaevis with homologous sperm and its verification using microsatellite markers
Auteurs : Xiang-Shan Ji ; Yong-Sheng Tian ; Jing-Feng Yang ; Peng-Fei Wu ; Yun-Liang Jiang ; Song-Lin ChenSource :
- Aquaculture Research [ 1355-557X ] ; 2010-05.
English descriptors
Abstract
Effective methods for induction of gynogenetic diploids in Cynoglossus semilaevis are needed to initiate monosex culture. An effective protocol to induce half‐smooth tongue sole gynogenesis using homologous sperm was developed in this study. A UV dose of 50 mJ cm−2 was found to be the most effective for genetic inactivation of tongue sole sperm. Treatment optima for cold shocks were 5 °C for 20–23 min at 5 min after fertilization and the hatching rate of gynogenetic diploids was 10.0%. Microsatellite analysis at locus Csou 6 revealed that there was no genetic contribution from the paternal genome in 24 progenies of a meiotic gynogenetic family. Polymerase chain reaction demonstrated that only four individuals of 24 meiotic gynogenetic diploids produced the female‐specific band of about 205 bp. The female/male ratio of gynogenetic diploids was significantly different from the theoretical ratio of 1:1. It is possible that there are some recessive lethal genes in W chromosome.
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DOI: 10.1111/j.1365-2109.2009.02373.x
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ISTEX:D44318BBCCB0DE78AF7DFFEBF4495CF62A56BBBFLe document en format XML
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An effective protocol to induce half‐smooth tongue sole gynogenesis using homologous sperm was developed in this study. A UV dose of 50 mJ cm−2 was found to be the most effective for genetic inactivation of tongue sole sperm. Treatment optima for cold shocks were 5 °C for 20–23 min at 5 min after fertilization and the hatching rate of gynogenetic diploids was 10.0%. Microsatellite analysis at locus Csou 6 revealed that there was no genetic contribution from the paternal genome in 24 progenies of a meiotic gynogenetic family. Polymerase chain reaction demonstrated that only four individuals of 24 meiotic gynogenetic diploids produced the female‐specific band of about 205 bp. The female/male ratio of gynogenetic diploids was significantly different from the theoretical ratio of 1:1. It is possible that there are some recessive lethal genes in W chromosome.</div></front></TEI><istex><corpusName>wiley</corpusName><author><json:item><name>Xiang‐Shan Ji</name><affiliations><json:string>Yellow Sea Fisheries Research Institute, Qingdao, China</json:string><json:string>College of Animal Science and Technology, Shandong Agricultural University, Taian, China</json:string></affiliations></json:item><json:item><name>Yong‐Sheng Tian</name><affiliations><json:string>Yellow Sea Fisheries Research Institute, Qingdao, China</json:string></affiliations></json:item><json:item><name>Jing‐Feng Yang</name><affiliations><json:string>Yellow Sea Fisheries Research Institute, Qingdao, China</json:string></affiliations></json:item><json:item><name>Peng‐Fei Wu</name><affiliations><json:string>Yellow Sea Fisheries Research Institute, Qingdao, China</json:string></affiliations></json:item><json:item><name>Yun‐Liang Jiang</name><affiliations><json:string>College of Animal Science and Technology, Shandong Agricultural University, Taian, China</json:string></affiliations></json:item><json:item><name>Song‐Lin Chen</name><affiliations><json:string>Yellow Sea Fisheries Research Institute, Qingdao, China</json:string></affiliations></json:item></author><subject><json:item><lang><json:string>eng</json:string></lang><value>Cynoglossus semilaevis</value></json:item><json:item><lang><json:string>eng</json:string></lang><value>gynogenesis</value></json:item><json:item><lang><json:string>eng</json:string></lang><value>ploidy analyser</value></json:item><json:item><lang><json:string>eng</json:string></lang><value>microsatellite</value></json:item></subject><articleId><json:string>ARE2373</json:string></articleId><language><json:string>eng</json:string></language><originalGenre><json:string>article</json:string></originalGenre><abstract>Effective methods for induction of gynogenetic diploids in Cynoglossus semilaevis are needed to initiate monosex culture. 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Journal Compilation © 2009 Blackwell Publishing Ltd</p></availability><date>2010</date></publicationStmt><sourceDesc><biblStruct type="inbook"><analytic><title level="a" type="main" xml:lang="en">Artificial gynogenesis in Cynoglossus semilaevis with homologous sperm and its verification using microsatellite markers</title><author xml:id="author-1"><persName><forename type="first">Xiang‐Shan</forename><surname>Ji</surname></persName><note type="biography">*Contributed equally to this work.</note><affiliation>*Contributed equally to this work.</affiliation><affiliation>Yellow Sea Fisheries Research Institute, Qingdao, China</affiliation><affiliation>College of Animal Science and Technology, Shandong Agricultural University, Taian, China</affiliation></author><author xml:id="author-2"><persName><forename type="first">Yong‐Sheng</forename><surname>Tian</surname></persName><note type="biography">*Contributed equally to this work.</note><affiliation>*Contributed equally to this work.</affiliation><affiliation>Yellow Sea Fisheries Research Institute, Qingdao, China</affiliation></author><author xml:id="author-3"><persName><forename type="first">Jing‐Feng</forename><surname>Yang</surname></persName><affiliation>Yellow Sea Fisheries Research Institute, Qingdao, China</affiliation></author><author xml:id="author-4"><persName><forename type="first">Peng‐Fei</forename><surname>Wu</surname></persName><affiliation>Yellow Sea Fisheries Research Institute, Qingdao, China</affiliation></author><author xml:id="author-5"><persName><forename type="first">Yun‐Liang</forename><surname>Jiang</surname></persName><affiliation>College of Animal Science and Technology, Shandong Agricultural University, Taian, China</affiliation></author><author xml:id="author-6"><persName><forename type="first">Song‐Lin</forename><surname>Chen</surname></persName><affiliation>Yellow Sea Fisheries Research Institute, Qingdao, China</affiliation></author></analytic><monogr><title level="j">Aquaculture Research</title><idno type="pISSN">1355-557X</idno><idno type="eISSN">1365-2109</idno><idno type="DOI">10.1111/(ISSN)1365-2109</idno><imprint><publisher>Blackwell Publishing Ltd</publisher><pubPlace>Oxford, UK</pubPlace><date type="published" when="2010-05"></date><biblScope unit="volume">41</biblScope><biblScope unit="issue">6</biblScope><biblScope unit="page" from="913">913</biblScope><biblScope unit="page" to="920">920</biblScope></imprint></monogr><idno type="istex">D44318BBCCB0DE78AF7DFFEBF4495CF62A56BBBF</idno><idno type="DOI">10.1111/j.1365-2109.2009.02373.x</idno><idno type="ArticleID">ARE2373</idno></biblStruct></sourceDesc></fileDesc><profileDesc><creation><date>2010</date></creation><langUsage><language ident="en">en</language></langUsage><abstract xml:lang="en"><p>Effective methods for induction of gynogenetic diploids in Cynoglossus semilaevis are needed to initiate monosex culture. An effective protocol to induce half‐smooth tongue sole gynogenesis using homologous sperm was developed in this study. A UV dose of 50 mJ cm−2 was found to be the most effective for genetic inactivation of tongue sole sperm. Treatment optima for cold shocks were 5 °C for 20–23 min at 5 min after fertilization and the hatching rate of gynogenetic diploids was 10.0%. Microsatellite analysis at locus Csou 6 revealed that there was no genetic contribution from the paternal genome in 24 progenies of a meiotic gynogenetic family. Polymerase chain reaction demonstrated that only four individuals of 24 meiotic gynogenetic diploids produced the female‐specific band of about 205 bp. The female/male ratio of gynogenetic diploids was significantly different from the theoretical ratio of 1:1. It is possible that there are some recessive lethal genes in W chromosome.</p></abstract><textClass xml:lang="en"><keywords scheme="keyword"><list><head>keywords</head><item><term>Cynoglossus semilaevis</term></item><item><term>gynogenesis</term></item><item><term>ploidy analyser</term></item><item><term>microsatellite</term></item></list></keywords></textClass></profileDesc><revisionDesc><change when="2010-05">Published</change></revisionDesc></teiHeader></istex:fulltextTEI><json:item><extension>txt</extension><original>false</original><mimetype>text/plain</mimetype><uri>https://api.istex.fr/document/D44318BBCCB0DE78AF7DFFEBF4495CF62A56BBBF/fulltext/txt</uri></json:item></fulltext><metadata><istex:metadataXml wicri:clean="Wiley, elements deleted: body"><istex:xmlDeclaration>version="1.0" encoding="UTF-8" standalone="yes"</istex:xmlDeclaration><istex:document><component version="2.0" type="serialArticle" xml:lang="en"><header><publicationMeta level="product"><publisherInfo><publisherName>Blackwell Publishing Ltd</publisherName><publisherLoc>Oxford, UK</publisherLoc></publisherInfo><doi origin="wiley" registered="yes">10.1111/(ISSN)1365-2109</doi><issn type="print">1355-557X</issn><issn type="electronic">1365-2109</issn><idGroup><id type="product" value="ARE"></id><id type="publisherDivision" value="ST"></id></idGroup><titleGroup><title type="main" sort="AQUACULTURE RESEARCH">Aquaculture Research</title></titleGroup></publicationMeta><publicationMeta level="part" position="05006"><doi origin="wiley">10.1111/are.2010.41.issue-6</doi><numberingGroup><numbering type="journalVolume" number="41">41</numbering><numbering type="journalIssue" number="6">6</numbering></numberingGroup><coverDate startDate="2010-05">May 2010</coverDate></publicationMeta><publicationMeta level="unit" type="article" position="13" status="forIssue"><doi origin="wiley">10.1111/j.1365-2109.2009.02373.x</doi><idGroup><id type="unit" value="ARE2373"></id><id type="supplier" value="2373"></id></idGroup><countGroup><count type="pageTotal" number="8"></count></countGroup><titleGroup><title type="tocHeading1">Original Articles</title></titleGroup><copyright>© 2009 The Authors. Journal Compilation © 2009 Blackwell Publishing Ltd</copyright><eventGroup><event type="firstOnline" date="2009-10-13"></event><event type="publishedOnlineFinalForm" date="2010-05-13"></event><event type="xmlConverted" agent="Converter:BPG_TO_WML3G version:2.3.6 mode:FullText source:FullText result:FullText" date="2010-05-17"></event><event type="xmlConverted" agent="Converter:WILEY_ML3G_TO_WILEY_ML3GV2 version:3.8.8" date="2014-01-06"></event><event type="xmlConverted" agent="Converter:WML3G_To_WML3G version:4.1.7 mode:FullText,remove_FC" date="2014-10-15"></event></eventGroup><numberingGroup><numbering type="pageFirst" number="913">913</numbering><numbering type="pageLast" number="920">920</numbering></numberingGroup><correspondenceTo><b>Correspondence:</b> Dr S‐L Chen, Yellow Sea Fisheries Research Institute, Chinese Academy of Fisheries Sciences, Nanjing Road 106, 266071 Qingdao, China. E‐mail: <email normalForm="chensl@ysfri.ac.cn">chensl@ysfri.ac.cn</email></correspondenceTo><linkGroup><link type="toTypesetVersion" href="file:ARE.ARE2373.pdf"></link></linkGroup></publicationMeta><contentMeta><countGroup><count type="figureTotal" number="4"></count><count type="tableTotal" number="2"></count><count type="formulaTotal" number="0"></count><count type="referenceTotal" number="24"></count><count type="wordTotal" number="4867"></count><count type="linksCrossRef" number="41"></count></countGroup><titleGroup><title type="main">Artificial gynogenesis in <i>Cynoglossus semilaevis</i> with homologous sperm and its verification using microsatellite markers</title><title type="shortAuthors">X‐S Ji <i>et al.</i></title><title type="short">Gynogenesis induction in half‐smooth tongue sole</title></titleGroup><creators><creator creatorRole="author" xml:id="cr1" affiliationRef="#a1 #a2" noteRef="#fn1"><personName><givenNames>Xiang‐Shan</givenNames><familyName>Ji</familyName></personName></creator><creator creatorRole="author" xml:id="cr2" affiliationRef="#a1" noteRef="#fn1"><personName><givenNames>Yong‐Sheng</givenNames><familyName>Tian</familyName></personName></creator><creator creatorRole="author" xml:id="cr3" affiliationRef="#a1"><personName><givenNames>Jing‐Feng</givenNames><familyName>Yang</familyName></personName></creator><creator creatorRole="author" xml:id="cr4" affiliationRef="#a1"><personName><givenNames>Peng‐Fei</givenNames><familyName>Wu</familyName></personName></creator><creator creatorRole="author" xml:id="cr5" affiliationRef="#a2"><personName><givenNames>Yun‐Liang</givenNames><familyName>Jiang</familyName></personName></creator><creator creatorRole="author" xml:id="cr6" affiliationRef="#a1"><personName><givenNames>Song‐Lin</givenNames><familyName>Chen</familyName></personName></creator></creators><affiliationGroup><affiliation xml:id="a1" countryCode="CN"><unparsedAffiliation>Yellow Sea Fisheries Research Institute, Qingdao, China</unparsedAffiliation></affiliation><affiliation xml:id="a2" countryCode="CN"><unparsedAffiliation>College of Animal Science and Technology, Shandong Agricultural University, Taian, China</unparsedAffiliation></affiliation></affiliationGroup><keywordGroup xml:lang="en"><keyword xml:id="k1"><i>Cynoglossus semilaevis</i></keyword><keyword xml:id="k2">gynogenesis</keyword><keyword xml:id="k3">ploidy analyser</keyword><keyword xml:id="k4">microsatellite</keyword></keywordGroup><abstractGroup><abstract type="main" xml:lang="en"><title type="main">Abstract</title><p>Effective methods for induction of gynogenetic diploids in <i>Cynoglossus semilaevis</i> are needed to initiate monosex culture. An effective protocol to induce half‐smooth tongue sole gynogenesis using homologous sperm was developed in this study. A UV dose of 50 mJ cm<sup>−2</sup> was found to be the most effective for genetic inactivation of tongue sole sperm. Treatment optima for cold shocks were 5 °C for 20–23 min at 5 min after fertilization and the hatching rate of gynogenetic diploids was 10.0%. Microsatellite analysis at locus <i>Csou 6</i> revealed that there was no genetic contribution from the paternal genome in 24 progenies of a meiotic gynogenetic family. Polymerase chain reaction demonstrated that only four individuals of 24 meiotic gynogenetic diploids produced the female‐specific band of about 205 bp. The female/male ratio of gynogenetic diploids was significantly different from the theoretical ratio of 1:1. It is possible that there are some recessive lethal genes in W chromosome.</p></abstract></abstractGroup></contentMeta><noteGroup><note xml:id="fn1" numbered="no"><p><sup>*</sup>Contributed equally to this work.</p></note></noteGroup></header></component></istex:document></istex:metadataXml><mods version="3.6"><titleInfo lang="en"><title>Artificial gynogenesis in Cynoglossus semilaevis with homologous sperm and its verification using microsatellite markers</title></titleInfo><titleInfo type="abbreviated" lang="en"><title>Gynogenesis induction in half‐smooth tongue sole</title></titleInfo><titleInfo type="alternative" contentType="CDATA" lang="en"><title>Artificial gynogenesis in Cynoglossus semilaevis with homologous sperm and its verification using microsatellite markers</title></titleInfo><name type="personal"><namePart type="given">Xiang‐Shan</namePart><namePart type="family">Ji</namePart><affiliation>Yellow Sea Fisheries Research Institute, Qingdao, China</affiliation><affiliation>College of Animal Science and Technology, Shandong Agricultural University, Taian, China</affiliation><description>*Contributed equally to this work.</description><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">Yong‐Sheng</namePart><namePart type="family">Tian</namePart><affiliation>Yellow Sea Fisheries Research Institute, Qingdao, China</affiliation><description>*Contributed equally to this work.</description><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">Jing‐Feng</namePart><namePart type="family">Yang</namePart><affiliation>Yellow Sea Fisheries Research Institute, Qingdao, China</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">Peng‐Fei</namePart><namePart type="family">Wu</namePart><affiliation>Yellow Sea Fisheries Research Institute, Qingdao, China</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">Yun‐Liang</namePart><namePart type="family">Jiang</namePart><affiliation>College of Animal Science and Technology, Shandong Agricultural University, Taian, China</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">Song‐Lin</namePart><namePart type="family">Chen</namePart><affiliation>Yellow Sea Fisheries Research Institute, Qingdao, China</affiliation><role><roleTerm type="text">author</roleTerm></role></name><typeOfResource>text</typeOfResource><genre type="article" displayLabel="article"></genre><originInfo><publisher>Blackwell Publishing Ltd</publisher><place><placeTerm type="text">Oxford, UK</placeTerm></place><dateIssued encoding="w3cdtf">2010-05</dateIssued><copyrightDate encoding="w3cdtf">2010</copyrightDate></originInfo><language><languageTerm type="code" authority="rfc3066">en</languageTerm><languageTerm type="code" authority="iso639-2b">eng</languageTerm></language><physicalDescription><internetMediaType>text/html</internetMediaType><extent unit="figures">4</extent><extent unit="tables">2</extent><extent unit="references">24</extent><extent unit="words">4867</extent></physicalDescription><abstract lang="en">Effective methods for induction of gynogenetic diploids in Cynoglossus semilaevis are needed to initiate monosex culture. An effective protocol to induce half‐smooth tongue sole gynogenesis using homologous sperm was developed in this study. A UV dose of 50 mJ cm−2 was found to be the most effective for genetic inactivation of tongue sole sperm. Treatment optima for cold shocks were 5 °C for 20–23 min at 5 min after fertilization and the hatching rate of gynogenetic diploids was 10.0%. Microsatellite analysis at locus Csou 6 revealed that there was no genetic contribution from the paternal genome in 24 progenies of a meiotic gynogenetic family. Polymerase chain reaction demonstrated that only four individuals of 24 meiotic gynogenetic diploids produced the female‐specific band of about 205 bp. The female/male ratio of gynogenetic diploids was significantly different from the theoretical ratio of 1:1. It is possible that there are some recessive lethal genes in W chromosome.</abstract><subject lang="en"><genre>keywords</genre><topic>Cynoglossus semilaevis</topic><topic>gynogenesis</topic><topic>ploidy analyser</topic><topic>microsatellite</topic></subject><relatedItem type="host"><titleInfo><title>Aquaculture Research</title></titleInfo><genre type="journal">journal</genre><identifier type="ISSN">1355-557X</identifier><identifier type="eISSN">1365-2109</identifier><identifier type="DOI">10.1111/(ISSN)1365-2109</identifier><identifier type="PublisherID">ARE</identifier><part><date>2010</date><detail type="volume"><caption>vol.</caption><number>41</number></detail><detail type="issue"><caption>no.</caption><number>6</number></detail><extent unit="pages"><start>913</start><end>920</end><total>8</total></extent></part></relatedItem><identifier type="istex">D44318BBCCB0DE78AF7DFFEBF4495CF62A56BBBF</identifier><identifier type="DOI">10.1111/j.1365-2109.2009.02373.x</identifier><identifier type="ArticleID">ARE2373</identifier><accessCondition type="use and reproduction" contentType="copyright">© 2009 The Authors. 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