EsturgeonV1, Main, Exploration, bibRecord, 001A00

Characterization of acrosin-like activity of lake sturgeon (Acipenser fulvescens) spermatozoa

Identifieur interne : 001A00 ( Main/Exploration ); précédent : 001999; suivant : 001A01

Characterization of acrosin-like activity of lake sturgeon (Acipenser fulvescens) spermatozoa

Auteurs : A. Ciereszko [États-Unis, Pologne] ; K. Dabrowski [États-Unis] ; S. I. Ochkur [Ukraine]

Source :

Molecular reproduction and development [ 1040-452X ] ; 1996.

RBID : Pascal:96-0429133

Descripteurs français

Pascal (Inist)
- Spermatozoïde, Acrosin, Trypsin, Homologie, Activité enzymatique, Régulation, Cryoconservation, Conservation sperme, Pisces, Mâle, Acipenser fulvescens.

English descriptors

KwdEn :
- Acrosin, Acrosin (chemistry), Animals, Benzoates (pharmacology), Benzoylarginine Nitroanilide (metabolism), Benzoylarginine Nitroanilide (pharmacology), Cryopreservation, Endopeptidases (chemistry), Endopeptidases (metabolism), Enzymatic activity, Enzyme Stability, Fishes, Guanidines (pharmacology), Homology, Kinetics, Male, Octoxynol (pharmacology), Pisces, Protease Inhibitors (pharmacology), Regulation(control), Semen (chemistry), Sperm conservation, Spermatozoa, Spermatozoa (enzymology), Temperature, Trypsin, Zinc (pharmacology).
MESH :
- chemical , chemistry : Acrosin, Endopeptidases.
- chemical , metabolism : Benzoylarginine Nitroanilide, Endopeptidases.
- chemical , pharmacology : Benzoates, Benzoylarginine Nitroanilide, Guanidines, Octoxynol, Protease Inhibitors, Zinc.
- chemistry : Semen.
- enzymology : Spermatozoa.
- Animals, Cryopreservation, Enzyme Stability, Fishes, Kinetics, Male, Temperature.

Abstract

Acipenserid fish sperm possess trypsin-like activity, resembling acrosin activity of mammalian sperm, which can be measured by hydrolysis of N-α-benzoyl-DL-arginine p-nitroanilide (BAPNA) (Ciereszko et al., 1994 : J Exp Zool 268 :486-491). We found that this activity can be preserved when sperm is frozen on dry ice with 0.6 M sucrose-10% dimethylsulfoxide (DMSO) extender (sperm :extender ratio 1 :3), and subsequently stored in liquid nitrogen. However, other methods of freezing (without cryoprotectant at -18°C, -80°C, and -196°C) did not protect this activity. Acrosin-like activity decreased in the course of storage of milt on ice ; 88% decline was recorded after 13 days. Acrosin-like activity increased with temperature from 10°C to 30°C, but was inactivated at 40°C to about 40% as compared to the optimum temperature. Triton X-100 inhibited activity by 15% and 72% at 0.01% and 0.1% concentrations, respectively. Activity was not affected by Mg²⁺ but was inhibited by Zn²⁺ (30% and 75% in the presence of 0.1 mM and 1 mM, respectively). Maximum velocity of substrate hydrolysis was observed at 2 mM of BAPNA. Acrosin-like activity was effectively inhibited by 4'-acetamidophenyl 4-guanidinobenzoate (AGB), an inhibitor of mammalian acrosin. Sperm acrosin-like activity correlated negatively with anti-proteinase activity of seminal plasma. We conclude that sturgeon acrosin-like activity shares many properties with mammalian acrosin. On the other hand, it has some unique properties which may represent adaptations of this enzyme to the environment of external fertilization.

Affiliations:

Pologne, Ukraine, États-Unis
Ohio

Le document en format XML

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<term>Benzoates (pharmacology)</term>
<term>Benzoylarginine Nitroanilide (metabolism)</term>
<term>Benzoylarginine Nitroanilide (pharmacology)</term>
<term>Cryopreservation</term>
<term>Endopeptidases (chemistry)</term>
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<term>Protease Inhibitors (pharmacology)</term>
<term>Regulation(control)</term>
<term>Semen (chemistry)</term>
<term>Sperm conservation</term>
<term>Spermatozoa</term>
<term>Spermatozoa (enzymology)</term>
<term>Temperature</term>
<term>Trypsin</term>
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<term>Guanidines</term>
<term>Octoxynol</term>
<term>Protease Inhibitors</term>
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<keywords scheme="MESH" xml:lang="en"><term>Animals</term>
<term>Cryopreservation</term>
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<term>Activité enzymatique</term>
<term>Régulation</term>
<term>Cryoconservation</term>
<term>Conservation sperme</term>
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<front><div type="abstract" xml:lang="en">Acipenserid fish sperm possess trypsin-like activity, resembling acrosin activity of mammalian sperm, which can be measured by hydrolysis of N-α-benzoyl-DL-arginine p-nitroanilide (BAPNA) (Ciereszko et al., 1994 : J Exp Zool 268 :486-491). We found that this activity can be preserved when sperm is frozen on dry ice with 0.6 M sucrose-10% dimethylsulfoxide (DMSO) extender (sperm :extender ratio 1 :3), and subsequently stored in liquid nitrogen. However, other methods of freezing (without cryoprotectant at -18°C, -80°C, and -196°C) did not protect this activity. Acrosin-like activity decreased in the course of storage of milt on ice ; 88% decline was recorded after 13 days. Acrosin-like activity increased with temperature from 10°C to 30°C, but was inactivated at 40°C to about 40% as compared to the optimum temperature. Triton X-100 inhibited activity by 15% and 72% at 0.01% and 0.1% concentrations, respectively. Activity was not affected by Mg<sup>2+</sup>
 but was inhibited by Zn<sup>2+</sup>
 (30% and 75% in the presence of 0.1 mM and 1 mM, respectively). Maximum velocity of substrate hydrolysis was observed at 2 mM of BAPNA. Acrosin-like activity was effectively inhibited by 4'-acetamidophenyl 4-guanidinobenzoate (AGB), an inhibitor of mammalian acrosin. Sperm acrosin-like activity correlated negatively with anti-proteinase activity of seminal plasma. We conclude that sturgeon acrosin-like activity shares many properties with mammalian acrosin. On the other hand, it has some unique properties which may represent adaptations of this enzyme to the environment of external fertilization.</div>
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Characterization of acrosin-like activity of lake sturgeon (Acipenser fulvescens) spermatozoa

Characterization of acrosin-like activity of lake sturgeon (Acipenser fulvescens) spermatozoa

Source :

Descripteurs français

English descriptors

Abstract

Links toward previous steps (curation, corpus...)

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