Characterization of acrosin-like activity of lake sturgeon (Acipenser fulvescens) spermatozoa.
Identifieur interne : 000684 ( PubMed/Corpus ); précédent : 000683; suivant : 000685Characterization of acrosin-like activity of lake sturgeon (Acipenser fulvescens) spermatozoa.
Auteurs : A. Ciereszko ; K. Dabrowski ; S I OchkurSource :
- Molecular reproduction and development [ 1040-452X ] ; 1996.
English descriptors
- KwdEn :
- Acrosin (chemistry), Animals, Benzoates (pharmacology), Benzoylarginine Nitroanilide (metabolism), Benzoylarginine Nitroanilide (pharmacology), Cryopreservation, Endopeptidases (chemistry), Endopeptidases (metabolism), Enzyme Stability, Fishes, Guanidines (pharmacology), Kinetics, Male, Octoxynol (pharmacology), Protease Inhibitors (pharmacology), Semen (chemistry), Spermatozoa (enzymology), Temperature, Zinc (pharmacology).
- MESH :
- chemical , chemistry : Acrosin, Endopeptidases.
- chemical , metabolism : Benzoylarginine Nitroanilide, Endopeptidases.
- chemical , pharmacology : Benzoates, Benzoylarginine Nitroanilide, Guanidines, Octoxynol, Protease Inhibitors, Zinc.
- chemistry : Semen.
- enzymology : Spermatozoa.
- Animals, Cryopreservation, Enzyme Stability, Fishes, Kinetics, Male, Temperature.
Abstract
Acipenserid fish sperm possess trypsin-like activity, resembling acrosin activity of mammalian sperm, which can be measured by hydrolysis of N-alpha-benzoyl-DL-arginine p-nitroanilide (BAPNA) (Ciereszko et al., 1994: J Exp Zool 268:486-491). We found that this activity can be preserved when sperm is frozen on dry ice with 0.6 M sucrose-10% dimethylsulfoxide (DMSO) extender (sperm:extender ratio 1:3), and subsequently stored in liquid nitrogen. However, other methods of freezing (without cryoprotectant at -18 degrees C, -80 degrees C, and -196 degrees C) did not protect this activity. Acrosin-like activity decreased in the course of storage of milt on ice; 88% decline was recorded after 13 days. Acrosin-like activity increased with temperature from 10 degrees C to 30 degrees C, but was inactivated at 40 degrees C to about 40% as compared to the optimum temperature. Triton X-100 inhibited activity by 15% and 72% at 0.01% and 0.1% concentrations, respectively. Activity was not affected by Mg2+ but was inhibited by Zn2+ (30% and 75% in the presence of 0.1 mM and 1 mM, respectively). Maximum velocity of substrate hydrolysis was observed at 2 mM of BAPNA. Acrosin-like activity was effectively inhibited by 4'-acetamidophenyl 4-guanidinobenzoate (AGB), an inhibitor of mammalian acrosin. Sperm acrosin-like activity correlated negatively with antiproteinase activity of seminal plasma. We conclude that sturgeon acrosin-like activity shares many properties with mammalian acrosin. On the other hand, it has some unique properties which may represent adaptations of this enzyme to the environment of external fertilization.
DOI: 10.1002/(SICI)1098-2795(199609)45:1<72::AID-MRD10>3.0.CO;2-Z
PubMed: 8873072
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<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">Characterization of acrosin-like activity of lake sturgeon (Acipenser fulvescens) spermatozoa.</title><author><name sortKey="Ciereszko, A" sort="Ciereszko, A" uniqKey="Ciereszko A" first="A" last="Ciereszko">A. Ciereszko</name><affiliation><nlm:affiliation>School of Natural Resources, Ohio State University, Columbus 43210, USA.</nlm:affiliation></affiliation></author><author><name sortKey="Dabrowski, K" sort="Dabrowski, K" uniqKey="Dabrowski K" first="K" last="Dabrowski">K. Dabrowski</name></author><author><name sortKey="Ochkur, S I" sort="Ochkur, S I" uniqKey="Ochkur S" first="S I" last="Ochkur">S I Ochkur</name></author></titleStmt><publicationStmt><idno type="wicri:source">PubMed</idno><date when="1996">1996</date><idno type="RBID">pubmed:8873072</idno><idno type="pmid">8873072</idno><idno type="doi">10.1002/(SICI)1098-2795(199609)45:1<72::AID-MRD10>3.0.CO;2-Z</idno><idno type="wicri:Area/PubMed/Corpus">000684</idno><idno type="wicri:explorRef" wicri:stream="PubMed" wicri:step="Corpus" wicri:corpus="PubMed">000684</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en">Characterization of acrosin-like activity of lake sturgeon (Acipenser fulvescens) spermatozoa.</title><author><name sortKey="Ciereszko, A" sort="Ciereszko, A" uniqKey="Ciereszko A" first="A" last="Ciereszko">A. Ciereszko</name><affiliation><nlm:affiliation>School of Natural Resources, Ohio State University, Columbus 43210, USA.</nlm:affiliation></affiliation></author><author><name sortKey="Dabrowski, K" sort="Dabrowski, K" uniqKey="Dabrowski K" first="K" last="Dabrowski">K. Dabrowski</name></author><author><name sortKey="Ochkur, S I" sort="Ochkur, S I" uniqKey="Ochkur S" first="S I" last="Ochkur">S I Ochkur</name></author></analytic><series><title level="j">Molecular reproduction and development</title><idno type="ISSN">1040-452X</idno><imprint><date when="1996" type="published">1996</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Acrosin (chemistry)</term><term>Animals</term><term>Benzoates (pharmacology)</term><term>Benzoylarginine Nitroanilide (metabolism)</term><term>Benzoylarginine Nitroanilide (pharmacology)</term><term>Cryopreservation</term><term>Endopeptidases (chemistry)</term><term>Endopeptidases (metabolism)</term><term>Enzyme Stability</term><term>Fishes</term><term>Guanidines (pharmacology)</term><term>Kinetics</term><term>Male</term><term>Octoxynol (pharmacology)</term><term>Protease Inhibitors (pharmacology)</term><term>Semen (chemistry)</term><term>Spermatozoa (enzymology)</term><term>Temperature</term><term>Zinc (pharmacology)</term></keywords><keywords scheme="MESH" type="chemical" qualifier="chemistry" xml:lang="en"><term>Acrosin</term><term>Endopeptidases</term></keywords><keywords scheme="MESH" type="chemical" qualifier="metabolism" xml:lang="en"><term>Benzoylarginine Nitroanilide</term><term>Endopeptidases</term></keywords><keywords scheme="MESH" type="chemical" qualifier="pharmacology" xml:lang="en"><term>Benzoates</term><term>Benzoylarginine Nitroanilide</term><term>Guanidines</term><term>Octoxynol</term><term>Protease Inhibitors</term><term>Zinc</term></keywords><keywords scheme="MESH" qualifier="chemistry" xml:lang="en"><term>Semen</term></keywords><keywords scheme="MESH" qualifier="enzymology" xml:lang="en"><term>Spermatozoa</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Animals</term><term>Cryopreservation</term><term>Enzyme Stability</term><term>Fishes</term><term>Kinetics</term><term>Male</term><term>Temperature</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Acipenserid fish sperm possess trypsin-like activity, resembling acrosin activity of mammalian sperm, which can be measured by hydrolysis of N-alpha-benzoyl-DL-arginine p-nitroanilide (BAPNA) (Ciereszko et al., 1994: J Exp Zool 268:486-491). We found that this activity can be preserved when sperm is frozen on dry ice with 0.6 M sucrose-10% dimethylsulfoxide (DMSO) extender (sperm:extender ratio 1:3), and subsequently stored in liquid nitrogen. However, other methods of freezing (without cryoprotectant at -18 degrees C, -80 degrees C, and -196 degrees C) did not protect this activity. Acrosin-like activity decreased in the course of storage of milt on ice; 88% decline was recorded after 13 days. Acrosin-like activity increased with temperature from 10 degrees C to 30 degrees C, but was inactivated at 40 degrees C to about 40% as compared to the optimum temperature. Triton X-100 inhibited activity by 15% and 72% at 0.01% and 0.1% concentrations, respectively. Activity was not affected by Mg2+ but was inhibited by Zn2+ (30% and 75% in the presence of 0.1 mM and 1 mM, respectively). Maximum velocity of substrate hydrolysis was observed at 2 mM of BAPNA. Acrosin-like activity was effectively inhibited by 4'-acetamidophenyl 4-guanidinobenzoate (AGB), an inhibitor of mammalian acrosin. Sperm acrosin-like activity correlated negatively with antiproteinase activity of seminal plasma. We conclude that sturgeon acrosin-like activity shares many properties with mammalian acrosin. On the other hand, it has some unique properties which may represent adaptations of this enzyme to the environment of external fertilization.</div></front></TEI><pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">8873072</PMID><DateCreated><Year>1997</Year><Month>01</Month><Day>08</Day></DateCreated><DateCompleted><Year>1997</Year><Month>01</Month><Day>08</Day></DateCompleted><DateRevised><Year>2013</Year><Month>11</Month><Day>21</Day></DateRevised><Article PubModel="Print"><Journal><ISSN IssnType="Print">1040-452X</ISSN><JournalIssue CitedMedium="Print"><Volume>45</Volume><Issue>1</Issue><PubDate><Year>1996</Year><Month>Sep</Month></PubDate></JournalIssue><Title>Molecular reproduction and development</Title><ISOAbbreviation>Mol. Reprod. Dev.</ISOAbbreviation></Journal><ArticleTitle>Characterization of acrosin-like activity of lake sturgeon (Acipenser fulvescens) spermatozoa.</ArticleTitle><Pagination><MedlinePgn>72-7</MedlinePgn></Pagination><Abstract><AbstractText>Acipenserid fish sperm possess trypsin-like activity, resembling acrosin activity of mammalian sperm, which can be measured by hydrolysis of N-alpha-benzoyl-DL-arginine p-nitroanilide (BAPNA) (Ciereszko et al., 1994: J Exp Zool 268:486-491). We found that this activity can be preserved when sperm is frozen on dry ice with 0.6 M sucrose-10% dimethylsulfoxide (DMSO) extender (sperm:extender ratio 1:3), and subsequently stored in liquid nitrogen. However, other methods of freezing (without cryoprotectant at -18 degrees C, -80 degrees C, and -196 degrees C) did not protect this activity. Acrosin-like activity decreased in the course of storage of milt on ice; 88% decline was recorded after 13 days. Acrosin-like activity increased with temperature from 10 degrees C to 30 degrees C, but was inactivated at 40 degrees C to about 40% as compared to the optimum temperature. Triton X-100 inhibited activity by 15% and 72% at 0.01% and 0.1% concentrations, respectively. Activity was not affected by Mg2+ but was inhibited by Zn2+ (30% and 75% in the presence of 0.1 mM and 1 mM, respectively). Maximum velocity of substrate hydrolysis was observed at 2 mM of BAPNA. Acrosin-like activity was effectively inhibited by 4'-acetamidophenyl 4-guanidinobenzoate (AGB), an inhibitor of mammalian acrosin. Sperm acrosin-like activity correlated negatively with antiproteinase activity of seminal plasma. We conclude that sturgeon acrosin-like activity shares many properties with mammalian acrosin. On the other hand, it has some unique properties which may represent adaptations of this enzyme to the environment of external fertilization.</AbstractText></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Ciereszko</LastName><ForeName>A</ForeName><Initials>A</Initials><AffiliationInfo><Affiliation>School of Natural Resources, Ohio State University, Columbus 43210, USA.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Dabrowski</LastName><ForeName>K</ForeName><Initials>K</Initials></Author><Author ValidYN="Y"><LastName>Ochkur</LastName><ForeName>S I</ForeName><Initials>SI</Initials></Author></AuthorList><Language>eng</Language><PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType><PublicationType UI="D013486">Research Support, U.S. Gov't, Non-P.H.S.</PublicationType></PublicationTypeList></Article><MedlineJournalInfo><Country>United States</Country><MedlineTA>Mol Reprod Dev</MedlineTA><NlmUniqueID>8903333</NlmUniqueID><ISSNLinking>1040-452X</ISSNLinking></MedlineJournalInfo><ChemicalList><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D001565">Benzoates</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D006146">Guanidines</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D011480">Protease Inhibitors</NameOfSubstance></Chemical><Chemical><RegistryNumber>79126-29-7</RegistryNumber><NameOfSubstance UI="C058095">4'-acetamidophenyl 4-guanidinobenzoate</NameOfSubstance></Chemical><Chemical><RegistryNumber>9002-93-1</RegistryNumber><NameOfSubstance UI="D017830">Octoxynol</NameOfSubstance></Chemical><Chemical><RegistryNumber>911-76-2</RegistryNumber><NameOfSubstance UI="D001586">Benzoylarginine Nitroanilide</NameOfSubstance></Chemical><Chemical><RegistryNumber>EC 3.4.-</RegistryNumber><NameOfSubstance UI="D010450">Endopeptidases</NameOfSubstance></Chemical><Chemical><RegistryNumber>EC 3.4.21.10</RegistryNumber><NameOfSubstance UI="D000176">Acrosin</NameOfSubstance></Chemical><Chemical><RegistryNumber>J41CSQ7QDS</RegistryNumber><NameOfSubstance UI="D015032">Zinc</NameOfSubstance></Chemical></ChemicalList><CitationSubset>IM</CitationSubset><MeshHeadingList><MeshHeading><DescriptorName UI="D000176" MajorTopicYN="N">Acrosin</DescriptorName><QualifierName UI="Q000737" MajorTopicYN="Y">chemistry</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D000818" MajorTopicYN="N">Animals</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D001565" MajorTopicYN="N">Benzoates</DescriptorName><QualifierName UI="Q000494" MajorTopicYN="N">pharmacology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D001586" MajorTopicYN="N">Benzoylarginine Nitroanilide</DescriptorName><QualifierName UI="Q000378" MajorTopicYN="N">metabolism</QualifierName><QualifierName UI="Q000494" MajorTopicYN="N">pharmacology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D015925" MajorTopicYN="N">Cryopreservation</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D010450" MajorTopicYN="N">Endopeptidases</DescriptorName><QualifierName UI="Q000737" MajorTopicYN="N">chemistry</QualifierName><QualifierName UI="Q000378" MajorTopicYN="N">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D004795" MajorTopicYN="N">Enzyme Stability</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D005399" MajorTopicYN="N">Fishes</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D006146" MajorTopicYN="N">Guanidines</DescriptorName><QualifierName UI="Q000494" MajorTopicYN="N">pharmacology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D007700" MajorTopicYN="N">Kinetics</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D008297" MajorTopicYN="N">Male</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D017830" MajorTopicYN="N">Octoxynol</DescriptorName><QualifierName UI="Q000494" MajorTopicYN="N">pharmacology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D011480" MajorTopicYN="N">Protease Inhibitors</DescriptorName><QualifierName UI="Q000494" MajorTopicYN="N">pharmacology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D012661" MajorTopicYN="N">Semen</DescriptorName><QualifierName UI="Q000737" MajorTopicYN="N">chemistry</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D013094" MajorTopicYN="N">Spermatozoa</DescriptorName><QualifierName UI="Q000201" MajorTopicYN="Y">enzymology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D013696" MajorTopicYN="N">Temperature</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D015032" MajorTopicYN="N">Zinc</DescriptorName><QualifierName UI="Q000494" 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