Culture of Differentiated Adult Rabbit Auricular Chondrocytes
Identifieur interne : 000F23 ( Main/Exploration ); précédent : 000F22; suivant : 000F24Culture of Differentiated Adult Rabbit Auricular Chondrocytes
Auteurs : V. Villar [Espagne] ; B. Colaço [Portugal] ; I. Calles-Venal [Espagne] ; J. G. Fernández-Álvarez [Espagne] ; M. Fernández-Caso [Espagne] ; J. M. Villar [Espagne]Source :
- Anatomia, Histologia, Embryologia [ 0340-2096 ] ; 2005-12.
Abstract
Chondrocytes dedifferentiate to a fibroblast‐like phenotype on plastic surfaces. Dedifferentiation is reversible if these cells are then cultured embedded in gels as alginate, agarose or collagen. Chondrocytes cultured in suspension on a non‐adherent surface are also known to form aggregates of differentiated cells. The knowledge of chondrocyte behavior in culture is relevant for tissue engineering purposes. In this report we describe a simple method to culture differentiated or redifferentiated rabbit auricular chondrocytes on plastic surfaces with a stable phenotype. When chondrocyte aggregates formed in suspension are next seeded on plastic surfaces, most of them attach to the plastic as round or polygonal cells, and this morphological differentiation, confirmed by the presence of type II collagen, is stable for long culture periods. We also report that the addition of aggregates to monolayer cultures of dedifferentiated chondrocytes results in their redifferentiation, as is shown by their morphological changes and the synthesis of type II collagen. Therefore, this simple method can be useful for the study of chondrocyte behavior on plastic surfaces and for redifferentiating previously proliferated chondrocytes in tissue engineering techniques. Furthermore, these results demonstrate that, in addition to culture conditions such as cell isolation method or cell‐density, chondrocyte behavior on plastic depends on the presence or absence of aggregates resulting from the dissociation process.
Url:
DOI: 10.1111/j.1439-0264.2005.00669_123.x
Affiliations:
Links toward previous steps (curation, corpus...)
- to stream Istex, to step Corpus: 001528
- to stream Istex, to step Curation: 001526
- to stream Istex, to step Checkpoint: 000834
- to stream Main, to step Merge: 000F69
- to stream Main, to step Curation: 000F23
Le document en format XML
<record><TEI wicri:istexFullTextTei="biblStruct"><teiHeader><fileDesc><titleStmt><title xml:lang="en">Culture of Differentiated Adult Rabbit Auricular Chondrocytes</title>
<author><name sortKey="Villar, V" sort="Villar, V" uniqKey="Villar V" first="V." last="Villar">V. Villar</name>
</author>
<author><name sortKey="Colaco, B" sort="Colaco, B" uniqKey="Colaco B" first="B." last="Colaço">B. Colaço</name>
</author>
<author><name sortKey="Calles Enal, I" sort="Calles Enal, I" uniqKey="Calles Enal I" first="I." last="Calles-Venal">I. Calles-Venal</name>
</author>
<author><name sortKey="Fernandez Varez, J G" sort="Fernandez Varez, J G" uniqKey="Fernandez Varez J" first="J. G." last="Fernández-Álvarez">J. G. Fernández-Álvarez</name>
</author>
<author><name sortKey="Fernandez Aso, M" sort="Fernandez Aso, M" uniqKey="Fernandez Aso M" first="M." last="Fernández-Caso">M. Fernández-Caso</name>
</author>
<author><name sortKey="Villar, J M" sort="Villar, J M" uniqKey="Villar J" first="J. M." last="Villar">J. M. Villar</name>
</author>
</titleStmt>
<publicationStmt><idno type="wicri:source">ISTEX</idno>
<idno type="RBID">ISTEX:7714BB7591E93B72830B18CEA0A9DE94FD4F3AE6</idno>
<date when="2005" year="2005">2005</date>
<idno type="doi">10.1111/j.1439-0264.2005.00669_123.x</idno>
<idno type="url">https://api.istex.fr/document/7714BB7591E93B72830B18CEA0A9DE94FD4F3AE6/fulltext/pdf</idno>
<idno type="wicri:Area/Istex/Corpus">001528</idno>
<idno type="wicri:explorRef" wicri:stream="Istex" wicri:step="Corpus" wicri:corpus="ISTEX">001528</idno>
<idno type="wicri:Area/Istex/Curation">001526</idno>
<idno type="wicri:Area/Istex/Checkpoint">000834</idno>
<idno type="wicri:explorRef" wicri:stream="Istex" wicri:step="Checkpoint">000834</idno>
<idno type="wicri:doubleKey">0340-2096:2005:Villar V:culture:of:differentiated</idno>
<idno type="wicri:Area/Main/Merge">000F69</idno>
<idno type="wicri:Area/Main/Curation">000F23</idno>
<idno type="wicri:Area/Main/Exploration">000F23</idno>
</publicationStmt>
<sourceDesc><biblStruct><analytic><title level="a" type="main" xml:lang="en">Culture of Differentiated Adult Rabbit Auricular Chondrocytes</title>
<author><name sortKey="Villar, V" sort="Villar, V" uniqKey="Villar V" first="V." last="Villar">V. Villar</name>
<affiliation wicri:level="1"><country xml:lang="fr">Espagne</country>
<wicri:regionArea>Facultad de Veterinaria, Universidad Alfonso X, Villanueva de la Cañada‐ Madrid</wicri:regionArea>
<wicri:noRegion>Villanueva de la Cañada‐ Madrid</wicri:noRegion>
</affiliation>
</author>
<author><name sortKey="Colaco, B" sort="Colaco, B" uniqKey="Colaco B" first="B." last="Colaço">B. Colaço</name>
<affiliation wicri:level="1"><country xml:lang="fr">Portugal</country>
<wicri:regionArea>Departamento de Zootécnia, Universidade de Trás‐os‐Montes e Alto Douro, Vila Real</wicri:regionArea>
<wicri:noRegion>Vila Real</wicri:noRegion>
</affiliation>
</author>
<author><name sortKey="Calles Enal, I" sort="Calles Enal, I" uniqKey="Calles Enal I" first="I." last="Calles-Venal">I. Calles-Venal</name>
<affiliation wicri:level="1"><country xml:lang="fr">Espagne</country>
<wicri:regionArea>Facultad de Veterinaria, Universidad de León</wicri:regionArea>
<wicri:noRegion>Universidad de León</wicri:noRegion>
</affiliation>
</author>
<author><name sortKey="Fernandez Varez, J G" sort="Fernandez Varez, J G" uniqKey="Fernandez Varez J" first="J. G." last="Fernández-Álvarez">J. G. Fernández-Álvarez</name>
<affiliation wicri:level="1"><country xml:lang="fr">Espagne</country>
<wicri:regionArea>Facultad de Veterinaria, Universidad de León</wicri:regionArea>
<wicri:noRegion>Universidad de León</wicri:noRegion>
</affiliation>
</author>
<author><name sortKey="Fernandez Aso, M" sort="Fernandez Aso, M" uniqKey="Fernandez Aso M" first="M." last="Fernández-Caso">M. Fernández-Caso</name>
<affiliation wicri:level="1"><country xml:lang="fr">Espagne</country>
<wicri:regionArea>Facultad de Veterinaria, Universidad de León</wicri:regionArea>
<wicri:noRegion>Universidad de León</wicri:noRegion>
</affiliation>
</author>
<author><name sortKey="Villar, J M" sort="Villar, J M" uniqKey="Villar J" first="J. M." last="Villar">J. M. Villar</name>
<affiliation wicri:level="1"><country xml:lang="fr">Espagne</country>
<wicri:regionArea>Facultad de Veterinaria, Universidad de León</wicri:regionArea>
<wicri:noRegion>Universidad de León</wicri:noRegion>
</affiliation>
</author>
</analytic>
<monogr></monogr>
<series><title level="j">Anatomia, Histologia, Embryologia</title>
<idno type="ISSN">0340-2096</idno>
<idno type="eISSN">1439-0264</idno>
<imprint><publisher>Blackwell Verlag GmbH</publisher>
<pubPlace>Berlin, Germany</pubPlace>
<date type="published" when="2005-12">2005-12</date>
<biblScope unit="volume">34</biblScope>
<biblScope unit="supplement">s1</biblScope>
<biblScope unit="page" from="54">54</biblScope>
<biblScope unit="page" to="54">54</biblScope>
</imprint>
<idno type="ISSN">0340-2096</idno>
</series>
<idno type="istex">7714BB7591E93B72830B18CEA0A9DE94FD4F3AE6</idno>
<idno type="DOI">10.1111/j.1439-0264.2005.00669_123.x</idno>
<idno type="ArticleID">AHE669_123_123</idno>
</biblStruct>
</sourceDesc>
<seriesStmt><idno type="ISSN">0340-2096</idno>
</seriesStmt>
</fileDesc>
<profileDesc><textClass></textClass>
<langUsage><language ident="en">en</language>
</langUsage>
</profileDesc>
</teiHeader>
<front><div type="abstract" xml:lang="en">Chondrocytes dedifferentiate to a fibroblast‐like phenotype on plastic surfaces. Dedifferentiation is reversible if these cells are then cultured embedded in gels as alginate, agarose or collagen. Chondrocytes cultured in suspension on a non‐adherent surface are also known to form aggregates of differentiated cells. The knowledge of chondrocyte behavior in culture is relevant for tissue engineering purposes. In this report we describe a simple method to culture differentiated or redifferentiated rabbit auricular chondrocytes on plastic surfaces with a stable phenotype. When chondrocyte aggregates formed in suspension are next seeded on plastic surfaces, most of them attach to the plastic as round or polygonal cells, and this morphological differentiation, confirmed by the presence of type II collagen, is stable for long culture periods. We also report that the addition of aggregates to monolayer cultures of dedifferentiated chondrocytes results in their redifferentiation, as is shown by their morphological changes and the synthesis of type II collagen. Therefore, this simple method can be useful for the study of chondrocyte behavior on plastic surfaces and for redifferentiating previously proliferated chondrocytes in tissue engineering techniques. Furthermore, these results demonstrate that, in addition to culture conditions such as cell isolation method or cell‐density, chondrocyte behavior on plastic depends on the presence or absence of aggregates resulting from the dissociation process.</div>
</front>
</TEI>
<affiliations><list><country><li>Espagne</li>
<li>Portugal</li>
</country>
</list>
<tree><country name="Espagne"><noRegion><name sortKey="Villar, V" sort="Villar, V" uniqKey="Villar V" first="V." last="Villar">V. Villar</name>
</noRegion>
<name sortKey="Calles Enal, I" sort="Calles Enal, I" uniqKey="Calles Enal I" first="I." last="Calles-Venal">I. Calles-Venal</name>
<name sortKey="Fernandez Aso, M" sort="Fernandez Aso, M" uniqKey="Fernandez Aso M" first="M." last="Fernández-Caso">M. Fernández-Caso</name>
<name sortKey="Fernandez Varez, J G" sort="Fernandez Varez, J G" uniqKey="Fernandez Varez J" first="J. G." last="Fernández-Álvarez">J. G. Fernández-Álvarez</name>
<name sortKey="Villar, J M" sort="Villar, J M" uniqKey="Villar J" first="J. M." last="Villar">J. M. Villar</name>
</country>
<country name="Portugal"><noRegion><name sortKey="Colaco, B" sort="Colaco, B" uniqKey="Colaco B" first="B." last="Colaço">B. Colaço</name>
</noRegion>
</country>
</tree>
</affiliations>
</record>
Pour manipuler ce document sous Unix (Dilib)
EXPLOR_STEP=$WICRI_ROOT/Wicri/Eau/explor/EsturgeonV1/Data/Main/Exploration
HfdSelect -h $EXPLOR_STEP/biblio.hfd -nk 000F23 | SxmlIndent | more
Ou
HfdSelect -h $EXPLOR_AREA/Data/Main/Exploration/biblio.hfd -nk 000F23 | SxmlIndent | more
Pour mettre un lien sur cette page dans le réseau Wicri
{{Explor lien |wiki= Wicri/Eau |area= EsturgeonV1 |flux= Main |étape= Exploration |type= RBID |clé= ISTEX:7714BB7591E93B72830B18CEA0A9DE94FD4F3AE6 |texte= Culture of Differentiated Adult Rabbit Auricular Chondrocytes }}
![]() | This area was generated with Dilib version V0.6.27. | ![]() |