Culture of Differentiated Adult Rabbit Auricular Chondrocytes
Identifieur interne : 001528 ( Istex/Corpus ); précédent : 001527; suivant : 001529Culture of Differentiated Adult Rabbit Auricular Chondrocytes
Auteurs : V. Villar ; B. Colaço ; I. Calles-Venal ; J. G. Fernández-Álvarez ; M. Fernández-Caso ; J. M. VillarSource :
- Anatomia, Histologia, Embryologia [ 0340-2096 ] ; 2005-12.
Abstract
Chondrocytes dedifferentiate to a fibroblast‐like phenotype on plastic surfaces. Dedifferentiation is reversible if these cells are then cultured embedded in gels as alginate, agarose or collagen. Chondrocytes cultured in suspension on a non‐adherent surface are also known to form aggregates of differentiated cells. The knowledge of chondrocyte behavior in culture is relevant for tissue engineering purposes. In this report we describe a simple method to culture differentiated or redifferentiated rabbit auricular chondrocytes on plastic surfaces with a stable phenotype. When chondrocyte aggregates formed in suspension are next seeded on plastic surfaces, most of them attach to the plastic as round or polygonal cells, and this morphological differentiation, confirmed by the presence of type II collagen, is stable for long culture periods. We also report that the addition of aggregates to monolayer cultures of dedifferentiated chondrocytes results in their redifferentiation, as is shown by their morphological changes and the synthesis of type II collagen. Therefore, this simple method can be useful for the study of chondrocyte behavior on plastic surfaces and for redifferentiating previously proliferated chondrocytes in tissue engineering techniques. Furthermore, these results demonstrate that, in addition to culture conditions such as cell isolation method or cell‐density, chondrocyte behavior on plastic depends on the presence or absence of aggregates resulting from the dissociation process.
Url:
DOI: 10.1111/j.1439-0264.2005.00669_123.x
Links to Exploration step
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<affiliation>Departamento de Zootécnia, Universidade de Trás‐os‐Montes e Alto Douro, Vila Real, Portugal</affiliation>
<role><roleTerm type="text">author</roleTerm>
</role>
</name>
<name type="personal"><namePart type="given">I.</namePart>
<namePart type="family">Calles‐Venal</namePart>
<affiliation>Facultad de Veterinaria, Universidad de León, Spain</affiliation>
<role><roleTerm type="text">author</roleTerm>
</role>
</name>
<name type="personal"><namePart type="given">J. G.</namePart>
<namePart type="family">Fernández‐Álvarez</namePart>
<affiliation>Facultad de Veterinaria, Universidad de León, Spain</affiliation>
<role><roleTerm type="text">author</roleTerm>
</role>
</name>
<name type="personal"><namePart type="given">M.</namePart>
<namePart type="family">Fernández‐Caso</namePart>
<affiliation>Facultad de Veterinaria, Universidad de León, Spain</affiliation>
<role><roleTerm type="text">author</roleTerm>
</role>
</name>
<name type="personal"><namePart type="given">J. M.</namePart>
<namePart type="family">Villar</namePart>
<affiliation>Facultad de Veterinaria, Universidad de León, Spain</affiliation>
<role><roleTerm type="text">author</roleTerm>
</role>
</name>
<typeOfResource>text</typeOfResource>
<genre type="abstract" displayLabel="abstract"></genre>
<originInfo><publisher>Blackwell Verlag GmbH</publisher>
<place><placeTerm type="text">Berlin, Germany</placeTerm>
</place>
<dateIssued encoding="w3cdtf">2005-12</dateIssued>
<copyrightDate encoding="w3cdtf">2005</copyrightDate>
</originInfo>
<language><languageTerm type="code" authority="rfc3066">en</languageTerm>
<languageTerm type="code" authority="iso639-2b">eng</languageTerm>
</language>
<physicalDescription><internetMediaType>text/html</internetMediaType>
</physicalDescription>
<abstract lang="en">Chondrocytes dedifferentiate to a fibroblast‐like phenotype on plastic surfaces. Dedifferentiation is reversible if these cells are then cultured embedded in gels as alginate, agarose or collagen. Chondrocytes cultured in suspension on a non‐adherent surface are also known to form aggregates of differentiated cells. The knowledge of chondrocyte behavior in culture is relevant for tissue engineering purposes. In this report we describe a simple method to culture differentiated or redifferentiated rabbit auricular chondrocytes on plastic surfaces with a stable phenotype. When chondrocyte aggregates formed in suspension are next seeded on plastic surfaces, most of them attach to the plastic as round or polygonal cells, and this morphological differentiation, confirmed by the presence of type II collagen, is stable for long culture periods. We also report that the addition of aggregates to monolayer cultures of dedifferentiated chondrocytes results in their redifferentiation, as is shown by their morphological changes and the synthesis of type II collagen. Therefore, this simple method can be useful for the study of chondrocyte behavior on plastic surfaces and for redifferentiating previously proliferated chondrocytes in tissue engineering techniques. Furthermore, these results demonstrate that, in addition to culture conditions such as cell isolation method or cell‐density, chondrocyte behavior on plastic depends on the presence or absence of aggregates resulting from the dissociation process.</abstract>
<relatedItem type="host"><titleInfo><title>Anatomia, Histologia, Embryologia</title>
</titleInfo>
<genre type="journal">journal</genre>
<identifier type="ISSN">0340-2096</identifier>
<identifier type="eISSN">1439-0264</identifier>
<identifier type="DOI">10.1111/(ISSN)1439-0264</identifier>
<identifier type="PublisherID">AHE</identifier>
<part><date>2005</date>
<detail type="volume"><caption>vol.</caption>
<number>34</number>
</detail>
<detail type="supplement"><caption>Suppl. no.</caption>
<number>s1</number>
</detail>
<extent unit="pages"><start>54</start>
<end>54</end>
</extent>
</part>
</relatedItem>
<identifier type="istex">7714BB7591E93B72830B18CEA0A9DE94FD4F3AE6</identifier>
<identifier type="DOI">10.1111/j.1439-0264.2005.00669_123.x</identifier>
<identifier type="ArticleID">AHE669_123_123</identifier>
<recordInfo><recordContentSource>WILEY</recordContentSource>
<recordOrigin>Blackwell Verlag GmbH</recordOrigin>
</recordInfo>
</mods>
</metadata>
<serie></serie>
</istex>
</record>
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