Molecular Cloning and Pharmacological Characterization of a Somatostatin Receptor Subtype in the Gymnotiform Fish Apteronotus albifrons
Identifieur interne : 001673 ( Main/Curation ); précédent : 001672; suivant : 001674Molecular Cloning and Pharmacological Characterization of a Somatostatin Receptor Subtype in the Gymnotiform Fish Apteronotus albifrons
Auteurs : Günther K. H. Zupanc ; Sandra Siehler [Suisse] ; Eugenia M. C. Jones ; Klaus Seuwen [Suisse] ; Hiroto Furuta ; Daniel Hoyer [Suisse] ; Hideki YanoSource :
- General and Comparative Endocrinology [ 0016-6480 ] ; 1999.
Abstract
The actions of the various forms of somatostatin (SRIF), including those of the tetradecapeptide SRIF14, are mediated by specific receptors. In mammals, five subtypes of SRIF receptors, termed sst1–5, have been cloned. Using a combination of reverse transcriptase-polymerase chain reaction and genomic library screening in the gymnotiform fish Apteronotus albifrons, a gene encoding the first-known nonmammalian SRIF receptor has been isolated. The deduced amino acid sequence displays 59% identity with the human sst3 receptor protein; hence, the gene is termed “Apteronotus sst3.” The predicted protein consists of 494 amino acid residues exhibiting a putative seven-transmembrane domain topology typical of G protein-coupled receptors. A signal corresponding to the Apteronotus sst3 receptor was detected in brain after amplification of poly(A)+-RNA by reverse transcriptase-polymerase chain reaction, but not by Northern blot analysis or in situ hybridization, suggesting a low level of expression. Membranes prepared from CCL39 cells stably expressing the Apteronotus sst3 receptor gene bound [125I][Leu8,d-Trp22,125 I-Tyr25]SRIF28 with high affinity and in a saturable manner (Bmax=4470 fmol/mg protein; pKD=10.5). SRIF14 and various synthetic SRIF receptor agonists produced a dose-dependent inhibition of radioligand binding, with the following rank order of potency: SRIF14 ≈ SRIF28 > BIM 23052 > octreotide > BIM 23056. Under low stringency conditions, an Apteronotus sst3 probe hybridized to multiple DNA fragments in HindIII or EcoRI digests of A. albifrons DNA, indicating that the Apteronotus sst3 receptor is a member of a larger family of Apteronotus SRIF receptors.
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DOI: 10.1006/gcen.1999.7316
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<front><div type="abstract" xml:lang="en">The actions of the various forms of somatostatin (SRIF), including those of the tetradecapeptide SRIF14, are mediated by specific receptors. In mammals, five subtypes of SRIF receptors, termed sst1–5, have been cloned. Using a combination of reverse transcriptase-polymerase chain reaction and genomic library screening in the gymnotiform fish Apteronotus albifrons, a gene encoding the first-known nonmammalian SRIF receptor has been isolated. The deduced amino acid sequence displays 59% identity with the human sst3 receptor protein; hence, the gene is termed “Apteronotus sst3.” The predicted protein consists of 494 amino acid residues exhibiting a putative seven-transmembrane domain topology typical of G protein-coupled receptors. A signal corresponding to the Apteronotus sst3 receptor was detected in brain after amplification of poly(A)+-RNA by reverse transcriptase-polymerase chain reaction, but not by Northern blot analysis or in situ hybridization, suggesting a low level of expression. Membranes prepared from CCL39 cells stably expressing the Apteronotus sst3 receptor gene bound [125I][Leu8,d-Trp22,125 I-Tyr25]SRIF28 with high affinity and in a saturable manner (Bmax=4470 fmol/mg protein; pKD=10.5). SRIF14 and various synthetic SRIF receptor agonists produced a dose-dependent inhibition of radioligand binding, with the following rank order of potency: SRIF14 ≈ SRIF28 > BIM 23052 > octreotide > BIM 23056. Under low stringency conditions, an Apteronotus sst3 probe hybridized to multiple DNA fragments in HindIII or EcoRI digests of A. albifrons DNA, indicating that the Apteronotus sst3 receptor is a member of a larger family of Apteronotus SRIF receptors.</div>
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