Molecular Cloning and Pharmacological Characterization of a Somatostatin Receptor Subtype in the Gymnotiform Fish Apteronotus albifrons
Identifieur interne : 000D67 ( Istex/Checkpoint ); précédent : 000D66; suivant : 000D68Molecular Cloning and Pharmacological Characterization of a Somatostatin Receptor Subtype in the Gymnotiform Fish Apteronotus albifrons
Auteurs : Günther K. H. Zupanc ; Sandra Siehler [Suisse] ; Eugenia M. C. Jones ; Klaus Seuwen [Suisse] ; Hiroto Furuta ; Daniel Hoyer [Suisse] ; Hideki YanoSource :
- General and Comparative Endocrinology [ 0016-6480 ] ; 1999.
Abstract
The actions of the various forms of somatostatin (SRIF), including those of the tetradecapeptide SRIF14, are mediated by specific receptors. In mammals, five subtypes of SRIF receptors, termed sst1–5, have been cloned. Using a combination of reverse transcriptase-polymerase chain reaction and genomic library screening in the gymnotiform fish Apteronotus albifrons, a gene encoding the first-known nonmammalian SRIF receptor has been isolated. The deduced amino acid sequence displays 59% identity with the human sst3 receptor protein; hence, the gene is termed “Apteronotus sst3.” The predicted protein consists of 494 amino acid residues exhibiting a putative seven-transmembrane domain topology typical of G protein-coupled receptors. A signal corresponding to the Apteronotus sst3 receptor was detected in brain after amplification of poly(A)+-RNA by reverse transcriptase-polymerase chain reaction, but not by Northern blot analysis or in situ hybridization, suggesting a low level of expression. Membranes prepared from CCL39 cells stably expressing the Apteronotus sst3 receptor gene bound [125I][Leu8,d-Trp22,125 I-Tyr25]SRIF28 with high affinity and in a saturable manner (Bmax=4470 fmol/mg protein; pKD=10.5). SRIF14 and various synthetic SRIF receptor agonists produced a dose-dependent inhibition of radioligand binding, with the following rank order of potency: SRIF14 ≈ SRIF28 > BIM 23052 > octreotide > BIM 23056. Under low stringency conditions, an Apteronotus sst3 probe hybridized to multiple DNA fragments in HindIII or EcoRI digests of A. albifrons DNA, indicating that the Apteronotus sst3 receptor is a member of a larger family of Apteronotus SRIF receptors.
Url:
DOI: 10.1006/gcen.1999.7316
Affiliations:
Links toward previous steps (curation, corpus...)
Links to Exploration step
ISTEX:A6A3C008B4D1EA869D48A60ADF5BA7F6D3A8683ELe document en format XML
<record><TEI wicri:istexFullTextTei="biblStruct"><teiHeader><fileDesc><titleStmt><title xml:lang="en">Molecular Cloning and Pharmacological Characterization of a Somatostatin Receptor Subtype in the Gymnotiform Fish Apteronotus albifrons</title><author><name sortKey="Zupanc, Gunther K H" sort="Zupanc, Gunther K H" uniqKey="Zupanc G" first="Günther K. H." last="Zupanc">Günther K. H. Zupanc</name></author><author><name sortKey="Siehler, Sandra" sort="Siehler, Sandra" uniqKey="Siehler S" first="Sandra" last="Siehler">Sandra Siehler</name></author><author><name sortKey="Jones, Eugenia M C" sort="Jones, Eugenia M C" uniqKey="Jones E" first="Eugenia M. C." last="Jones">Eugenia M. C. Jones</name></author><author><name sortKey="Seuwen, Klaus" sort="Seuwen, Klaus" uniqKey="Seuwen K" first="Klaus" last="Seuwen">Klaus Seuwen</name></author><author><name sortKey="Furuta, Hiroto" sort="Furuta, Hiroto" uniqKey="Furuta H" first="Hiroto" last="Furuta">Hiroto Furuta</name></author><author><name sortKey="Hoyer, Daniel" sort="Hoyer, Daniel" uniqKey="Hoyer D" first="Daniel" last="Hoyer">Daniel Hoyer</name></author><author><name sortKey="Yano, Hideki" sort="Yano, Hideki" uniqKey="Yano H" first="Hideki" last="Yano">Hideki Yano</name></author></titleStmt><publicationStmt><idno type="wicri:source">ISTEX</idno><idno type="RBID">ISTEX:A6A3C008B4D1EA869D48A60ADF5BA7F6D3A8683E</idno><date when="1999" year="1999">1999</date><idno type="doi">10.1006/gcen.1999.7316</idno><idno type="url">https://api.istex.fr/document/A6A3C008B4D1EA869D48A60ADF5BA7F6D3A8683E/fulltext/pdf</idno><idno type="wicri:Area/Istex/Corpus">001174</idno><idno type="wicri:explorRef" wicri:stream="Istex" wicri:step="Corpus" wicri:corpus="ISTEX">001174</idno><idno type="wicri:Area/Istex/Curation">001172</idno><idno type="wicri:Area/Istex/Checkpoint">000D67</idno><idno type="wicri:explorRef" wicri:stream="Istex" wicri:step="Checkpoint">000D67</idno></publicationStmt><sourceDesc><biblStruct><analytic><title level="a" type="main" xml:lang="en">Molecular Cloning and Pharmacological Characterization of a Somatostatin Receptor Subtype in the Gymnotiform Fish Apteronotus albifrons</title><author><name sortKey="Zupanc, Gunther K H" sort="Zupanc, Gunther K H" uniqKey="Zupanc G" first="Günther K. H." last="Zupanc">Günther K. H. Zupanc</name><affiliation><wicri:noCountry code="subField">60637</wicri:noCountry></affiliation></author><author><name sortKey="Siehler, Sandra" sort="Siehler, Sandra" uniqKey="Siehler S" first="Sandra" last="Siehler">Sandra Siehler</name><affiliation wicri:level="1"><country xml:lang="fr">Suisse</country><wicri:regionArea>Nervous System Research, Novartis Pharma AG, CH-4002, Basel</wicri:regionArea><wicri:noRegion>Basel</wicri:noRegion></affiliation></author><author><name sortKey="Jones, Eugenia M C" sort="Jones, Eugenia M C" uniqKey="Jones E" first="Eugenia M. C." last="Jones">Eugenia M. C. Jones</name><affiliation><wicri:noCountry code="subField">60637</wicri:noCountry></affiliation></author><author><name sortKey="Seuwen, Klaus" sort="Seuwen, Klaus" uniqKey="Seuwen K" first="Klaus" last="Seuwen">Klaus Seuwen</name><affiliation wicri:level="1"><country xml:lang="fr">Suisse</country><wicri:regionArea>Nervous System Research, Novartis Pharma AG, CH-4002, Basel</wicri:regionArea><wicri:noRegion>Basel</wicri:noRegion></affiliation></author><author><name sortKey="Furuta, Hiroto" sort="Furuta, Hiroto" uniqKey="Furuta H" first="Hiroto" last="Furuta">Hiroto Furuta</name><affiliation><wicri:noCountry code="subField">60637</wicri:noCountry></affiliation></author><author><name sortKey="Hoyer, Daniel" sort="Hoyer, Daniel" uniqKey="Hoyer D" first="Daniel" last="Hoyer">Daniel Hoyer</name><affiliation wicri:level="1"><country xml:lang="fr">Suisse</country><wicri:regionArea>Nervous System Research, Novartis Pharma AG, CH-4002, Basel</wicri:regionArea><wicri:noRegion>Basel</wicri:noRegion></affiliation></author><author><name sortKey="Yano, Hideki" sort="Yano, Hideki" uniqKey="Yano H" first="Hideki" last="Yano">Hideki Yano</name><affiliation><wicri:noCountry code="subField">60637</wicri:noCountry></affiliation></author></analytic><monogr></monogr><series><title level="j">General and Comparative Endocrinology</title><title level="j" type="abbrev">YGCEN</title><idno type="ISSN">0016-6480</idno><imprint><publisher>ELSEVIER</publisher><date type="published" when="1999">1999</date><biblScope unit="volume">115</biblScope><biblScope unit="issue">3</biblScope><biblScope unit="page" from="333">333</biblScope><biblScope unit="page" to="345">345</biblScope></imprint><idno type="ISSN">0016-6480</idno></series><idno type="istex">A6A3C008B4D1EA869D48A60ADF5BA7F6D3A8683E</idno><idno type="DOI">10.1006/gcen.1999.7316</idno><idno type="PII">S0016-6480(99)97316-1</idno></biblStruct></sourceDesc><seriesStmt><idno type="ISSN">0016-6480</idno></seriesStmt></fileDesc><profileDesc><textClass></textClass><langUsage><language ident="en">en</language></langUsage></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">The actions of the various forms of somatostatin (SRIF), including those of the tetradecapeptide SRIF14, are mediated by specific receptors. In mammals, five subtypes of SRIF receptors, termed sst1–5, have been cloned. Using a combination of reverse transcriptase-polymerase chain reaction and genomic library screening in the gymnotiform fish Apteronotus albifrons, a gene encoding the first-known nonmammalian SRIF receptor has been isolated. The deduced amino acid sequence displays 59% identity with the human sst3 receptor protein; hence, the gene is termed “Apteronotus sst3.” The predicted protein consists of 494 amino acid residues exhibiting a putative seven-transmembrane domain topology typical of G protein-coupled receptors. A signal corresponding to the Apteronotus sst3 receptor was detected in brain after amplification of poly(A)+-RNA by reverse transcriptase-polymerase chain reaction, but not by Northern blot analysis or in situ hybridization, suggesting a low level of expression. Membranes prepared from CCL39 cells stably expressing the Apteronotus sst3 receptor gene bound [125I][Leu8,d-Trp22,125 I-Tyr25]SRIF28 with high affinity and in a saturable manner (Bmax=4470 fmol/mg protein; pKD=10.5). SRIF14 and various synthetic SRIF receptor agonists produced a dose-dependent inhibition of radioligand binding, with the following rank order of potency: SRIF14 ≈ SRIF28 > BIM 23052 > octreotide > BIM 23056. Under low stringency conditions, an Apteronotus sst3 probe hybridized to multiple DNA fragments in HindIII or EcoRI digests of A. albifrons DNA, indicating that the Apteronotus sst3 receptor is a member of a larger family of Apteronotus SRIF receptors.</div></front></TEI><affiliations><list><country><li>Suisse</li></country></list><tree><noCountry><name sortKey="Furuta, Hiroto" sort="Furuta, Hiroto" uniqKey="Furuta H" first="Hiroto" last="Furuta">Hiroto Furuta</name><name sortKey="Jones, Eugenia M C" sort="Jones, Eugenia M C" uniqKey="Jones E" first="Eugenia M. C." last="Jones">Eugenia M. C. Jones</name><name sortKey="Yano, Hideki" sort="Yano, Hideki" uniqKey="Yano H" first="Hideki" last="Yano">Hideki Yano</name><name sortKey="Zupanc, Gunther K H" sort="Zupanc, Gunther K H" uniqKey="Zupanc G" first="Günther K. H." last="Zupanc">Günther K. H. Zupanc</name></noCountry><country name="Suisse"><noRegion><name sortKey="Siehler, Sandra" sort="Siehler, Sandra" uniqKey="Siehler S" first="Sandra" last="Siehler">Sandra Siehler</name></noRegion><name sortKey="Hoyer, Daniel" sort="Hoyer, Daniel" uniqKey="Hoyer D" first="Daniel" last="Hoyer">Daniel Hoyer</name><name sortKey="Seuwen, Klaus" sort="Seuwen, Klaus" uniqKey="Seuwen K" first="Klaus" last="Seuwen">Klaus Seuwen</name></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
EXPLOR_STEP=$WICRI_ROOT/Wicri/Eau/explor/EsturgeonV1/Data/Istex/Checkpoint
HfdSelect -h $EXPLOR_STEP/biblio.hfd -nk 000D67 | SxmlIndent | more
Ou
HfdSelect -h $EXPLOR_AREA/Data/Istex/Checkpoint/biblio.hfd -nk 000D67 | SxmlIndent | more
Pour mettre un lien sur cette page dans le réseau Wicri
{{Explor lien
|wiki= Wicri/Eau
|area= EsturgeonV1
|flux= Istex
|étape= Checkpoint
|type= RBID
|clé= ISTEX:A6A3C008B4D1EA869D48A60ADF5BA7F6D3A8683E
|texte= Molecular Cloning and Pharmacological Characterization of a Somatostatin Receptor Subtype in the Gymnotiform Fish Apteronotus albifrons
}}
| This area was generated with Dilib version V0.6.27. |  |