A new species of nucleo-cytoplasmic large DNA virus (NCLDV) associated with mortalities in Manitoba lake sturgeon Acipenser fulvescens.
Identifieur interne : 000307 ( Main/Curation ); précédent : 000306; suivant : 000308A new species of nucleo-cytoplasmic large DNA virus (NCLDV) associated with mortalities in Manitoba lake sturgeon Acipenser fulvescens.
Auteurs : Sharon C. Clouthier [Canada] ; Elissa Vanwalleghem ; Shelagh Copeland ; Cheryl Klassen ; Gary Hobbs ; Ole Nielsen ; Eric D. AndersonSource :
- Diseases of aquatic organisms [ 0177-5103 ] ; 2013.
English descriptors
- KwdEn :
- Amino Acid Sequence, Animals, Base Sequence, Conservation of Natural Resources, DNA Virus Infections (mortality), DNA Virus Infections (pathology), DNA Virus Infections (veterinary), DNA Virus Infections (virology), DNA Viruses (classification), DNA Viruses (isolation & purification), DNA, Viral (genetics), Fish Diseases (mortality), Fish Diseases (pathology), Fish Diseases (virology), Fishes, Gene Expression Regulation, Viral (physiology), Lakes, Manitoba, Molecular Sequence Annotation, Phylogeny, Polymerase Chain Reaction, Viral Proteins (genetics), Viral Proteins (metabolism).
- MESH :
- chemical , genetics : DNA, Viral, Viral Proteins.
- chemical , metabolism : Viral Proteins.
- geographic : Manitoba.
- classification : DNA Viruses.
- isolation & purification : DNA Viruses.
- mortality : DNA Virus Infections, Fish Diseases.
- pathology : DNA Virus Infections, Fish Diseases.
- physiology : Gene Expression Regulation, Viral.
- veterinary : DNA Virus Infections.
- virology : DNA Virus Infections, Fish Diseases.
- Amino Acid Sequence, Animals, Base Sequence, Conservation of Natural Resources, Fishes, Lakes, Molecular Sequence Annotation, Phylogeny, Polymerase Chain Reaction.
Abstract
A newly discovered virus, Namao virus, associated with morbidity and mortality, was detected among juvenile lake sturgeon Acipenser fulvescens being propagated by a conservation stocking program for this endangered species in Manitoba, Canada. The outbreaks resulted in cumulative mortalities of 62 to 99.6% among progeny of wild Winnipeg River or Nelson River lake sturgeon and occurred at 2 geographically separate facilities. Namao virus was detected in almost 94% of the moribund or dead lake sturgeon according to a conventional polymerase chain reaction (cPCR) test that is based upon amplification of a 219 bp fragment of the virus major capsid protein (MCP). The virus itself was large (242 to 282 nm) and icosahedral-shaped with 2 capsids and a condensed bar-shaped core. It was found in virus factories within the host cell cytoplasm and displayed a tropism for the integument. Namao virus caused cellular changes characterized by enlarged eosinophilic epithelial cells in the gills and skin. Samples suspected of containing Namao virus did not have cytopathic effects on primary lake sturgeon or established white sturgeon cell lines. However, viral nucleic acid was detected in the former after prolonged incubation periods. Using primers designed from conserved regions of the MCP from NCLDVs, an estimated 95 to 96% of the Namao virus MCP open reading frame was captured. Phylogenetic analysis using the MCP of Namao virus and 27 other NCLDVs suggested that Namao virus and white sturgeon iridovirus share a common evolutionary past and might be members of the family Mimiviridae or a new, as yet unrecognized, virus family.
DOI: 10.3354/dao02548
PubMed: 23446969
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pubmed:23446969Le document en format XML
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Clouthier</name><affiliation wicri:level="1"><nlm:affiliation>Freshwater Institute, Fisheries & Oceans Canada, Winnipeg, Manitoba, Canada.</nlm:affiliation><country xml:lang="fr">Canada</country><wicri:regionArea>Freshwater Institute, Fisheries & Oceans Canada, Winnipeg, Manitoba</wicri:regionArea><wicri:noRegion>Manitoba</wicri:noRegion></affiliation></author><author><name sortKey="Vanwalleghem, Elissa" sort="Vanwalleghem, Elissa" uniqKey="Vanwalleghem E" first="Elissa" last="Vanwalleghem">Elissa Vanwalleghem</name></author><author><name sortKey="Copeland, Shelagh" sort="Copeland, Shelagh" uniqKey="Copeland S" first="Shelagh" last="Copeland">Shelagh Copeland</name></author><author><name sortKey="Klassen, Cheryl" sort="Klassen, Cheryl" uniqKey="Klassen C" first="Cheryl" last="Klassen">Cheryl Klassen</name></author><author><name sortKey="Hobbs, Gary" sort="Hobbs, Gary" uniqKey="Hobbs G" first="Gary" last="Hobbs">Gary Hobbs</name></author><author><name sortKey="Nielsen, Ole" sort="Nielsen, Ole" uniqKey="Nielsen O" first="Ole" last="Nielsen">Ole Nielsen</name></author><author><name sortKey="Anderson, Eric D" sort="Anderson, Eric D" uniqKey="Anderson E" first="Eric D" last="Anderson">Eric D. Anderson</name></author></titleStmt><publicationStmt><idno type="wicri:source">PubMed</idno><date when="2013">2013</date><idno type="RBID">pubmed:23446969</idno><idno type="pmid">23446969</idno><idno type="doi">10.3354/dao02548</idno><idno type="wicri:Area/PubMed/Corpus">000270</idno><idno type="wicri:explorRef" wicri:stream="PubMed" wicri:step="Corpus" wicri:corpus="PubMed">000270</idno><idno type="wicri:Area/PubMed/Curation">000270</idno><idno type="wicri:explorRef" wicri:stream="PubMed" wicri:step="Curation">000270</idno><idno type="wicri:Area/PubMed/Checkpoint">000270</idno><idno type="wicri:explorRef" wicri:stream="Checkpoint" wicri:step="PubMed">000270</idno><idno type="wicri:Area/Ncbi/Merge">000470</idno><idno type="wicri:Area/Ncbi/Curation">000470</idno><idno type="wicri:Area/Ncbi/Checkpoint">000470</idno><idno type="wicri:doubleKey">0177-5103:2013:Clouthier S:a:new:species</idno><idno type="wicri:Area/Main/Merge">000308</idno><idno type="wicri:Area/Main/Curation">000307</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en">A new species of nucleo-cytoplasmic large DNA virus (NCLDV) associated with mortalities in Manitoba lake sturgeon Acipenser fulvescens.</title><author><name sortKey="Clouthier, Sharon C" sort="Clouthier, Sharon C" uniqKey="Clouthier S" first="Sharon C" last="Clouthier">Sharon C. Clouthier</name><affiliation wicri:level="1"><nlm:affiliation>Freshwater Institute, Fisheries & Oceans Canada, Winnipeg, Manitoba, Canada.</nlm:affiliation><country xml:lang="fr">Canada</country><wicri:regionArea>Freshwater Institute, Fisheries & Oceans Canada, Winnipeg, Manitoba</wicri:regionArea><wicri:noRegion>Manitoba</wicri:noRegion></affiliation></author><author><name sortKey="Vanwalleghem, Elissa" sort="Vanwalleghem, Elissa" uniqKey="Vanwalleghem E" first="Elissa" last="Vanwalleghem">Elissa Vanwalleghem</name></author><author><name sortKey="Copeland, Shelagh" sort="Copeland, Shelagh" uniqKey="Copeland S" first="Shelagh" last="Copeland">Shelagh Copeland</name></author><author><name sortKey="Klassen, Cheryl" sort="Klassen, Cheryl" uniqKey="Klassen C" first="Cheryl" last="Klassen">Cheryl Klassen</name></author><author><name sortKey="Hobbs, Gary" sort="Hobbs, Gary" uniqKey="Hobbs G" first="Gary" last="Hobbs">Gary Hobbs</name></author><author><name sortKey="Nielsen, Ole" sort="Nielsen, Ole" uniqKey="Nielsen O" first="Ole" last="Nielsen">Ole Nielsen</name></author><author><name sortKey="Anderson, Eric D" sort="Anderson, Eric D" uniqKey="Anderson E" first="Eric D" last="Anderson">Eric D. Anderson</name></author></analytic><series><title level="j">Diseases of aquatic organisms</title><idno type="ISSN">0177-5103</idno><imprint><date when="2013" type="published">2013</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Amino Acid Sequence</term><term>Animals</term><term>Base Sequence</term><term>Conservation of Natural Resources</term><term>DNA Virus Infections (mortality)</term><term>DNA Virus Infections (pathology)</term><term>DNA Virus Infections (veterinary)</term><term>DNA Virus Infections (virology)</term><term>DNA Viruses (classification)</term><term>DNA Viruses (isolation & purification)</term><term>DNA, Viral (genetics)</term><term>Fish Diseases (mortality)</term><term>Fish Diseases (pathology)</term><term>Fish Diseases (virology)</term><term>Fishes</term><term>Gene Expression Regulation, Viral (physiology)</term><term>Lakes</term><term>Manitoba</term><term>Molecular Sequence Annotation</term><term>Phylogeny</term><term>Polymerase Chain Reaction</term><term>Viral Proteins (genetics)</term><term>Viral Proteins (metabolism)</term></keywords><keywords scheme="MESH" type="chemical" qualifier="genetics" xml:lang="en"><term>DNA, Viral</term><term>Viral Proteins</term></keywords><keywords scheme="MESH" type="chemical" qualifier="metabolism" xml:lang="en"><term>Viral Proteins</term></keywords><keywords scheme="MESH" type="geographic" xml:lang="en"><term>Manitoba</term></keywords><keywords scheme="MESH" qualifier="classification" xml:lang="en"><term>DNA Viruses</term></keywords><keywords scheme="MESH" qualifier="isolation & purification" xml:lang="en"><term>DNA Viruses</term></keywords><keywords scheme="MESH" qualifier="mortality" xml:lang="en"><term>DNA Virus Infections</term><term>Fish Diseases</term></keywords><keywords scheme="MESH" qualifier="pathology" xml:lang="en"><term>DNA Virus Infections</term><term>Fish Diseases</term></keywords><keywords scheme="MESH" qualifier="physiology" xml:lang="en"><term>Gene Expression Regulation, Viral</term></keywords><keywords scheme="MESH" qualifier="veterinary" xml:lang="en"><term>DNA Virus Infections</term></keywords><keywords scheme="MESH" qualifier="virology" xml:lang="en"><term>DNA Virus Infections</term><term>Fish Diseases</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Amino Acid Sequence</term><term>Animals</term><term>Base Sequence</term><term>Conservation of Natural Resources</term><term>Fishes</term><term>Lakes</term><term>Molecular Sequence Annotation</term><term>Phylogeny</term><term>Polymerase Chain Reaction</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">A newly discovered virus, Namao virus, associated with morbidity and mortality, was detected among juvenile lake sturgeon Acipenser fulvescens being propagated by a conservation stocking program for this endangered species in Manitoba, Canada. The outbreaks resulted in cumulative mortalities of 62 to 99.6% among progeny of wild Winnipeg River or Nelson River lake sturgeon and occurred at 2 geographically separate facilities. Namao virus was detected in almost 94% of the moribund or dead lake sturgeon according to a conventional polymerase chain reaction (cPCR) test that is based upon amplification of a 219 bp fragment of the virus major capsid protein (MCP). The virus itself was large (242 to 282 nm) and icosahedral-shaped with 2 capsids and a condensed bar-shaped core. It was found in virus factories within the host cell cytoplasm and displayed a tropism for the integument. Namao virus caused cellular changes characterized by enlarged eosinophilic epithelial cells in the gills and skin. Samples suspected of containing Namao virus did not have cytopathic effects on primary lake sturgeon or established white sturgeon cell lines. However, viral nucleic acid was detected in the former after prolonged incubation periods. Using primers designed from conserved regions of the MCP from NCLDVs, an estimated 95 to 96% of the Namao virus MCP open reading frame was captured. Phylogenetic analysis using the MCP of Namao virus and 27 other NCLDVs suggested that Namao virus and white sturgeon iridovirus share a common evolutionary past and might be members of the family Mimiviridae or a new, as yet unrecognized, virus family.</div></front></TEI></record>Pour manipuler ce document sous Unix (Dilib)
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