A new species of nucleo-cytoplasmic large DNA virus (NCLDV) associated with mortalities in Manitoba lake sturgeon Acipenser fulvescens.
Identifieur interne : 000270 ( PubMed/Corpus ); précédent : 000269; suivant : 000271A new species of nucleo-cytoplasmic large DNA virus (NCLDV) associated with mortalities in Manitoba lake sturgeon Acipenser fulvescens.
Auteurs : Sharon C. Clouthier ; Elissa Vanwalleghem ; Shelagh Copeland ; Cheryl Klassen ; Gary Hobbs ; Ole Nielsen ; Eric D. AndersonSource :
- Diseases of aquatic organisms [ 0177-5103 ] ; 2013.
English descriptors
- KwdEn :
- Amino Acid Sequence, Animals, Base Sequence, Conservation of Natural Resources, DNA Virus Infections (mortality), DNA Virus Infections (pathology), DNA Virus Infections (veterinary), DNA Virus Infections (virology), DNA Viruses (classification), DNA Viruses (isolation & purification), DNA, Viral (genetics), Fish Diseases (mortality), Fish Diseases (pathology), Fish Diseases (virology), Fishes, Gene Expression Regulation, Viral (physiology), Lakes, Manitoba, Molecular Sequence Annotation, Phylogeny, Polymerase Chain Reaction, Viral Proteins (genetics), Viral Proteins (metabolism).
- MESH :
- chemical , genetics : DNA, Viral, Viral Proteins.
- chemical , metabolism : Viral Proteins.
- geographic : Manitoba.
- classification : DNA Viruses.
- isolation & purification : DNA Viruses.
- mortality : DNA Virus Infections, Fish Diseases.
- pathology : DNA Virus Infections, Fish Diseases.
- physiology : Gene Expression Regulation, Viral.
- veterinary : DNA Virus Infections.
- virology : DNA Virus Infections, Fish Diseases.
- Amino Acid Sequence, Animals, Base Sequence, Conservation of Natural Resources, Fishes, Lakes, Molecular Sequence Annotation, Phylogeny, Polymerase Chain Reaction.
Abstract
A newly discovered virus, Namao virus, associated with morbidity and mortality, was detected among juvenile lake sturgeon Acipenser fulvescens being propagated by a conservation stocking program for this endangered species in Manitoba, Canada. The outbreaks resulted in cumulative mortalities of 62 to 99.6% among progeny of wild Winnipeg River or Nelson River lake sturgeon and occurred at 2 geographically separate facilities. Namao virus was detected in almost 94% of the moribund or dead lake sturgeon according to a conventional polymerase chain reaction (cPCR) test that is based upon amplification of a 219 bp fragment of the virus major capsid protein (MCP). The virus itself was large (242 to 282 nm) and icosahedral-shaped with 2 capsids and a condensed bar-shaped core. It was found in virus factories within the host cell cytoplasm and displayed a tropism for the integument. Namao virus caused cellular changes characterized by enlarged eosinophilic epithelial cells in the gills and skin. Samples suspected of containing Namao virus did not have cytopathic effects on primary lake sturgeon or established white sturgeon cell lines. However, viral nucleic acid was detected in the former after prolonged incubation periods. Using primers designed from conserved regions of the MCP from NCLDVs, an estimated 95 to 96% of the Namao virus MCP open reading frame was captured. Phylogenetic analysis using the MCP of Namao virus and 27 other NCLDVs suggested that Namao virus and white sturgeon iridovirus share a common evolutionary past and might be members of the family Mimiviridae or a new, as yet unrecognized, virus family.
DOI: 10.3354/dao02548
PubMed: 23446969
Links to Exploration step
pubmed:23446969Le document en format XML
<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">A new species of nucleo-cytoplasmic large DNA virus (NCLDV) associated with mortalities in Manitoba lake sturgeon Acipenser fulvescens.</title><author><name sortKey="Clouthier, Sharon C" sort="Clouthier, Sharon C" uniqKey="Clouthier S" first="Sharon C" last="Clouthier">Sharon C. Clouthier</name><affiliation><nlm:affiliation>Freshwater Institute, Fisheries & Oceans Canada, Winnipeg, Manitoba, Canada.</nlm:affiliation></affiliation></author><author><name sortKey="Vanwalleghem, Elissa" sort="Vanwalleghem, Elissa" uniqKey="Vanwalleghem E" first="Elissa" last="Vanwalleghem">Elissa Vanwalleghem</name></author><author><name sortKey="Copeland, Shelagh" sort="Copeland, Shelagh" uniqKey="Copeland S" first="Shelagh" last="Copeland">Shelagh Copeland</name></author><author><name sortKey="Klassen, Cheryl" sort="Klassen, Cheryl" uniqKey="Klassen C" first="Cheryl" last="Klassen">Cheryl Klassen</name></author><author><name sortKey="Hobbs, Gary" sort="Hobbs, Gary" uniqKey="Hobbs G" first="Gary" last="Hobbs">Gary Hobbs</name></author><author><name sortKey="Nielsen, Ole" sort="Nielsen, Ole" uniqKey="Nielsen O" first="Ole" last="Nielsen">Ole Nielsen</name></author><author><name sortKey="Anderson, Eric D" sort="Anderson, Eric D" uniqKey="Anderson E" first="Eric D" last="Anderson">Eric D. Anderson</name></author></titleStmt><publicationStmt><idno type="wicri:source">PubMed</idno><date when="2013">2013</date><idno type="RBID">pubmed:23446969</idno><idno type="pmid">23446969</idno><idno type="doi">10.3354/dao02548</idno><idno type="wicri:Area/PubMed/Corpus">000270</idno><idno type="wicri:explorRef" wicri:stream="PubMed" wicri:step="Corpus" wicri:corpus="PubMed">000270</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en">A new species of nucleo-cytoplasmic large DNA virus (NCLDV) associated with mortalities in Manitoba lake sturgeon Acipenser fulvescens.</title><author><name sortKey="Clouthier, Sharon C" sort="Clouthier, Sharon C" uniqKey="Clouthier S" first="Sharon C" last="Clouthier">Sharon C. Clouthier</name><affiliation><nlm:affiliation>Freshwater Institute, Fisheries & Oceans Canada, Winnipeg, Manitoba, Canada.</nlm:affiliation></affiliation></author><author><name sortKey="Vanwalleghem, Elissa" sort="Vanwalleghem, Elissa" uniqKey="Vanwalleghem E" first="Elissa" last="Vanwalleghem">Elissa Vanwalleghem</name></author><author><name sortKey="Copeland, Shelagh" sort="Copeland, Shelagh" uniqKey="Copeland S" first="Shelagh" last="Copeland">Shelagh Copeland</name></author><author><name sortKey="Klassen, Cheryl" sort="Klassen, Cheryl" uniqKey="Klassen C" first="Cheryl" last="Klassen">Cheryl Klassen</name></author><author><name sortKey="Hobbs, Gary" sort="Hobbs, Gary" uniqKey="Hobbs G" first="Gary" last="Hobbs">Gary Hobbs</name></author><author><name sortKey="Nielsen, Ole" sort="Nielsen, Ole" uniqKey="Nielsen O" first="Ole" last="Nielsen">Ole Nielsen</name></author><author><name sortKey="Anderson, Eric D" sort="Anderson, Eric D" uniqKey="Anderson E" first="Eric D" last="Anderson">Eric D. Anderson</name></author></analytic><series><title level="j">Diseases of aquatic organisms</title><idno type="ISSN">0177-5103</idno><imprint><date when="2013" type="published">2013</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Amino Acid Sequence</term><term>Animals</term><term>Base Sequence</term><term>Conservation of Natural Resources</term><term>DNA Virus Infections (mortality)</term><term>DNA Virus Infections (pathology)</term><term>DNA Virus Infections (veterinary)</term><term>DNA Virus Infections (virology)</term><term>DNA Viruses (classification)</term><term>DNA Viruses (isolation & purification)</term><term>DNA, Viral (genetics)</term><term>Fish Diseases (mortality)</term><term>Fish Diseases (pathology)</term><term>Fish Diseases (virology)</term><term>Fishes</term><term>Gene Expression Regulation, Viral (physiology)</term><term>Lakes</term><term>Manitoba</term><term>Molecular Sequence Annotation</term><term>Phylogeny</term><term>Polymerase Chain Reaction</term><term>Viral Proteins (genetics)</term><term>Viral Proteins (metabolism)</term></keywords><keywords scheme="MESH" type="chemical" qualifier="genetics" xml:lang="en"><term>DNA, Viral</term><term>Viral Proteins</term></keywords><keywords scheme="MESH" type="chemical" qualifier="metabolism" xml:lang="en"><term>Viral Proteins</term></keywords><keywords scheme="MESH" type="geographic" xml:lang="en"><term>Manitoba</term></keywords><keywords scheme="MESH" qualifier="classification" xml:lang="en"><term>DNA Viruses</term></keywords><keywords scheme="MESH" qualifier="isolation & purification" xml:lang="en"><term>DNA Viruses</term></keywords><keywords scheme="MESH" qualifier="mortality" xml:lang="en"><term>DNA Virus Infections</term><term>Fish Diseases</term></keywords><keywords scheme="MESH" qualifier="pathology" xml:lang="en"><term>DNA Virus Infections</term><term>Fish Diseases</term></keywords><keywords scheme="MESH" qualifier="physiology" xml:lang="en"><term>Gene Expression Regulation, Viral</term></keywords><keywords scheme="MESH" qualifier="veterinary" xml:lang="en"><term>DNA Virus Infections</term></keywords><keywords scheme="MESH" qualifier="virology" xml:lang="en"><term>DNA Virus Infections</term><term>Fish Diseases</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Amino Acid Sequence</term><term>Animals</term><term>Base Sequence</term><term>Conservation of Natural Resources</term><term>Fishes</term><term>Lakes</term><term>Molecular Sequence Annotation</term><term>Phylogeny</term><term>Polymerase Chain Reaction</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">A newly discovered virus, Namao virus, associated with morbidity and mortality, was detected among juvenile lake sturgeon Acipenser fulvescens being propagated by a conservation stocking program for this endangered species in Manitoba, Canada. The outbreaks resulted in cumulative mortalities of 62 to 99.6% among progeny of wild Winnipeg River or Nelson River lake sturgeon and occurred at 2 geographically separate facilities. Namao virus was detected in almost 94% of the moribund or dead lake sturgeon according to a conventional polymerase chain reaction (cPCR) test that is based upon amplification of a 219 bp fragment of the virus major capsid protein (MCP). The virus itself was large (242 to 282 nm) and icosahedral-shaped with 2 capsids and a condensed bar-shaped core. It was found in virus factories within the host cell cytoplasm and displayed a tropism for the integument. Namao virus caused cellular changes characterized by enlarged eosinophilic epithelial cells in the gills and skin. Samples suspected of containing Namao virus did not have cytopathic effects on primary lake sturgeon or established white sturgeon cell lines. However, viral nucleic acid was detected in the former after prolonged incubation periods. Using primers designed from conserved regions of the MCP from NCLDVs, an estimated 95 to 96% of the Namao virus MCP open reading frame was captured. Phylogenetic analysis using the MCP of Namao virus and 27 other NCLDVs suggested that Namao virus and white sturgeon iridovirus share a common evolutionary past and might be members of the family Mimiviridae or a new, as yet unrecognized, virus family.</div></front></TEI><pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">23446969</PMID><DateCreated><Year>2013</Year><Month>02</Month><Day>28</Day></DateCreated><DateCompleted><Year>2013</Year><Month>06</Month><Day>20</Day></DateCompleted><DateRevised><Year>2013</Year><Month>02</Month><Day>28</Day></DateRevised><Article PubModel="Print"><Journal><ISSN IssnType="Print">0177-5103</ISSN><JournalIssue CitedMedium="Print"><Volume>102</Volume><Issue>3</Issue><PubDate><Year>2013</Year><Month>Feb</Month><Day>28</Day></PubDate></JournalIssue><Title>Diseases of aquatic organisms</Title><ISOAbbreviation>Dis. Aquat. Org.</ISOAbbreviation></Journal><ArticleTitle>A new species of nucleo-cytoplasmic large DNA virus (NCLDV) associated with mortalities in Manitoba lake sturgeon Acipenser fulvescens.</ArticleTitle><Pagination><MedlinePgn>195-209</MedlinePgn></Pagination><ELocationID EIdType="doi" ValidYN="Y">10.3354/dao02548</ELocationID><Abstract><AbstractText>A newly discovered virus, Namao virus, associated with morbidity and mortality, was detected among juvenile lake sturgeon Acipenser fulvescens being propagated by a conservation stocking program for this endangered species in Manitoba, Canada. The outbreaks resulted in cumulative mortalities of 62 to 99.6% among progeny of wild Winnipeg River or Nelson River lake sturgeon and occurred at 2 geographically separate facilities. Namao virus was detected in almost 94% of the moribund or dead lake sturgeon according to a conventional polymerase chain reaction (cPCR) test that is based upon amplification of a 219 bp fragment of the virus major capsid protein (MCP). The virus itself was large (242 to 282 nm) and icosahedral-shaped with 2 capsids and a condensed bar-shaped core. It was found in virus factories within the host cell cytoplasm and displayed a tropism for the integument. Namao virus caused cellular changes characterized by enlarged eosinophilic epithelial cells in the gills and skin. Samples suspected of containing Namao virus did not have cytopathic effects on primary lake sturgeon or established white sturgeon cell lines. However, viral nucleic acid was detected in the former after prolonged incubation periods. Using primers designed from conserved regions of the MCP from NCLDVs, an estimated 95 to 96% of the Namao virus MCP open reading frame was captured. Phylogenetic analysis using the MCP of Namao virus and 27 other NCLDVs suggested that Namao virus and white sturgeon iridovirus share a common evolutionary past and might be members of the family Mimiviridae or a new, as yet unrecognized, virus family.</AbstractText></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Clouthier</LastName><ForeName>Sharon C</ForeName><Initials>SC</Initials><AffiliationInfo><Affiliation>Freshwater Institute, Fisheries & Oceans Canada, Winnipeg, Manitoba, Canada.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Vanwalleghem</LastName><ForeName>Elissa</ForeName><Initials>E</Initials></Author><Author ValidYN="Y"><LastName>Copeland</LastName><ForeName>Shelagh</ForeName><Initials>S</Initials></Author><Author ValidYN="Y"><LastName>Klassen</LastName><ForeName>Cheryl</ForeName><Initials>C</Initials></Author><Author ValidYN="Y"><LastName>Hobbs</LastName><ForeName>Gary</ForeName><Initials>G</Initials></Author><Author ValidYN="Y"><LastName>Nielsen</LastName><ForeName>Ole</ForeName><Initials>O</Initials></Author><Author ValidYN="Y"><LastName>Anderson</LastName><ForeName>Eric D</ForeName><Initials>ED</Initials></Author></AuthorList><Language>eng</Language><PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType><PublicationType UI="D013485">Research Support, Non-U.S. Gov't</PublicationType></PublicationTypeList></Article><MedlineJournalInfo><Country>Germany</Country><MedlineTA>Dis Aquat Organ</MedlineTA><NlmUniqueID>8807037</NlmUniqueID><ISSNLinking>0177-5103</ISSNLinking></MedlineJournalInfo><ChemicalList><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D004279">DNA, Viral</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D014764">Viral Proteins</NameOfSubstance></Chemical></ChemicalList><CitationSubset>IM</CitationSubset><MeshHeadingList><MeshHeading><DescriptorName UI="D000595" MajorTopicYN="N">Amino Acid Sequence</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D000818" MajorTopicYN="N">Animals</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D001483" MajorTopicYN="N">Base Sequence</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D003247" MajorTopicYN="N">Conservation of Natural Resources</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D004266" MajorTopicYN="N">DNA Virus Infections</DescriptorName><QualifierName UI="Q000401" MajorTopicYN="N">mortality</QualifierName><QualifierName UI="Q000473" MajorTopicYN="N">pathology</QualifierName><QualifierName UI="Q000662" MajorTopicYN="Y">veterinary</QualifierName><QualifierName UI="Q000821" MajorTopicYN="N">virology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D004267" MajorTopicYN="N">DNA Viruses</DescriptorName><QualifierName UI="Q000145" MajorTopicYN="Y">classification</QualifierName><QualifierName UI="Q000302" MajorTopicYN="Y">isolation & purification</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D004279" MajorTopicYN="N">DNA, Viral</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D005393" MajorTopicYN="N">Fish Diseases</DescriptorName><QualifierName UI="Q000401" MajorTopicYN="N">mortality</QualifierName><QualifierName UI="Q000473" MajorTopicYN="N">pathology</QualifierName><QualifierName UI="Q000821" MajorTopicYN="Y">virology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D005399" MajorTopicYN="N">Fishes</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D015967" MajorTopicYN="N">Gene Expression Regulation, Viral</DescriptorName><QualifierName UI="Q000502" MajorTopicYN="N">physiology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D060106" MajorTopicYN="N">Lakes</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D008350" MajorTopicYN="N" Type="Geographic">Manitoba</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D058977" MajorTopicYN="N">Molecular Sequence Annotation</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D010802" MajorTopicYN="N">Phylogeny</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D016133" MajorTopicYN="N">Polymerase Chain Reaction</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D014764" MajorTopicYN="N">Viral Proteins</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName><QualifierName UI="Q000378" MajorTopicYN="N">metabolism</QualifierName></MeshHeading></MeshHeadingList></MedlineCitation><PubmedData><History><PubMedPubDate PubStatus="entrez"><Year>2013</Year><Month>3</Month><Day>1</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="pubmed"><Year>2013</Year><Month>3</Month><Day>1</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="medline"><Year>2013</Year><Month>6</Month><Day>21</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate></History><PublicationStatus>ppublish</PublicationStatus><ArticleIdList><ArticleId IdType="pubmed">23446969</ArticleId><ArticleId IdType="doi">10.3354/dao02548</ArticleId></ArticleIdList></PubmedData></pubmed></record>Pour manipuler ce document sous Unix (Dilib)
EXPLOR_STEP=$WICRI_ROOT/Wicri/Eau/explor/EsturgeonV1/Data/PubMed/Corpus
HfdSelect -h $EXPLOR_STEP/biblio.hfd -nk 000270 | SxmlIndent | more
Ou
HfdSelect -h $EXPLOR_AREA/Data/PubMed/Corpus/biblio.hfd -nk 000270 | SxmlIndent | more
Pour mettre un lien sur cette page dans le réseau Wicri
{{Explor lien
|wiki= Wicri/Eau
|area= EsturgeonV1
|flux= PubMed
|étape= Corpus
|type= RBID
|clé= pubmed:23446969
|texte= A new species of nucleo-cytoplasmic large DNA virus (NCLDV) associated with mortalities in Manitoba lake sturgeon Acipenser fulvescens.
}}
Pour générer des pages wiki
HfdIndexSelect -h $EXPLOR_AREA/Data/PubMed/Corpus/RBID.i -Sk "pubmed:23446969" \
| HfdSelect -Kh $EXPLOR_AREA/Data/PubMed/Corpus/biblio.hfd \
| NlmPubMed2Wicri -a EsturgeonV1
| This area was generated with Dilib version V0.6.27. |  |