Cryopreservation of Persian sturgeon (Acipenser persicus) embryos by DMSO-based vitrificant solutions.
Identifieur interne : 000086 ( Main/Curation ); précédent : 000085; suivant : 000087Cryopreservation of Persian sturgeon (Acipenser persicus) embryos by DMSO-based vitrificant solutions.
Auteurs : Saeide Keivanloo [Iran] ; Mohammad Sudagar [Iran]Source :
- Theriogenology [ 1879-3231 ] ; 2016.
English descriptors
- KwdEn :
- MESH :
- chemical , pharmacology : Cryoprotective Agents, Dimethyl Sulfoxide.
- drug effects : Cell Survival, Embryonic Development, Vitrification.
- embryology : Fishes.
- methods : Cryopreservation.
- veterinary : Cryopreservation.
- Animals, Embryo, Nonmammalian, Freezing.
Abstract
Vitrification could provide a promising tool for the cryopreservation of fish embryos. To achieve successful cryopreservation, several parameters should be taken into account in the design of a vitrification protocol. In the present study, some relevant factors were investigated (choice of a proper vitrificant solutions and temperature for thawing) using neurulation-stage embryos. Six DMSO-based vitrificant solutions (V1-V6) were tested using a 6-step incorporation protocol. DMSO-based vitrificant solutions contained DMSO + permeable cryoprotectants + nonpermeable cryoprotectants. Embryos were immersed in vitrificant solutions for 7 minutes and directly plunged into liquid nitrogen. After vitrification (-196 °C for 10 minutes), the thawing was performed in a water bath at 0 or 20 °C and then embryos incubated until hatched. Our results demonstrated that some embryos vitrified in 5 of 6 vitrification solutions survived and hatched out, but none survived after vitrification in V2. The highest survival rate (45.45%) was observed in samples frozen with the best vitrificant solution (V6) and thawing combination (20 °C). These results establish that cryopreservation of Persian sturgeon (Acipenser persicus) embryos by DMSO-based vitrificant solutions is possible.
DOI: 10.1016/j.theriogenology.2015.11.012
PubMed: 26768541
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pubmed:26768541Le document en format XML
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<affiliation wicri:level="1"><nlm:affiliation>Department of Fishery Sciences, Gorgan University of Agricultural Sciences and Natural Resources, Gorgan, Iran. Electronic address: skeivanloo@gau.ac.ir.</nlm:affiliation>
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<author><name sortKey="Keivanloo, Saeide" sort="Keivanloo, Saeide" uniqKey="Keivanloo S" first="Saeide" last="Keivanloo">Saeide Keivanloo</name>
<affiliation wicri:level="1"><nlm:affiliation>Department of Fishery Sciences, Gorgan University of Agricultural Sciences and Natural Resources, Gorgan, Iran. Electronic address: skeivanloo@gau.ac.ir.</nlm:affiliation>
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<author><name sortKey="Sudagar, Mohammad" sort="Sudagar, Mohammad" uniqKey="Sudagar M" first="Mohammad" last="Sudagar">Mohammad Sudagar</name>
<affiliation wicri:level="1"><nlm:affiliation>Department of Fishery Sciences, Gorgan University of Agricultural Sciences and Natural Resources, Gorgan, Iran.</nlm:affiliation>
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<series><title level="j">Theriogenology</title>
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<imprint><date when="2016" type="published">2016</date>
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<term>Cell Survival (drug effects)</term>
<term>Cryopreservation (methods)</term>
<term>Cryopreservation (veterinary)</term>
<term>Cryoprotective Agents (pharmacology)</term>
<term>Dimethyl Sulfoxide (pharmacology)</term>
<term>Embryo, Nonmammalian</term>
<term>Embryonic Development (drug effects)</term>
<term>Fishes (embryology)</term>
<term>Freezing</term>
<term>Vitrification (drug effects)</term>
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<term>Vitrification</term>
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<keywords scheme="MESH" qualifier="veterinary" xml:lang="en"><term>Cryopreservation</term>
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<front><div type="abstract" xml:lang="en">Vitrification could provide a promising tool for the cryopreservation of fish embryos. To achieve successful cryopreservation, several parameters should be taken into account in the design of a vitrification protocol. In the present study, some relevant factors were investigated (choice of a proper vitrificant solutions and temperature for thawing) using neurulation-stage embryos. Six DMSO-based vitrificant solutions (V1-V6) were tested using a 6-step incorporation protocol. DMSO-based vitrificant solutions contained DMSO + permeable cryoprotectants + nonpermeable cryoprotectants. Embryos were immersed in vitrificant solutions for 7 minutes and directly plunged into liquid nitrogen. After vitrification (-196 °C for 10 minutes), the thawing was performed in a water bath at 0 or 20 °C and then embryos incubated until hatched. Our results demonstrated that some embryos vitrified in 5 of 6 vitrification solutions survived and hatched out, but none survived after vitrification in V2. The highest survival rate (45.45%) was observed in samples frozen with the best vitrificant solution (V6) and thawing combination (20 °C). These results establish that cryopreservation of Persian sturgeon (Acipenser persicus) embryos by DMSO-based vitrificant solutions is possible.</div>
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