Cryopreservation of Persian sturgeon (Acipenser persicus) embryos by DMSO-based vitrificant solutions.
Identifieur interne : 000077 ( PubMed/Curation ); précédent : 000076; suivant : 000078Cryopreservation of Persian sturgeon (Acipenser persicus) embryos by DMSO-based vitrificant solutions.
Auteurs : Saeide Keivanloo [Iran] ; Mohammad Sudagar [Iran]Source :
- Theriogenology [ 1879-3231 ] ; 2016.
English descriptors
- KwdEn :
- MESH :
- chemical , pharmacology : Cryoprotective Agents, Dimethyl Sulfoxide.
- drug effects : Cell Survival, Embryonic Development, Vitrification.
- embryology : Fishes.
- methods : Cryopreservation.
- veterinary : Cryopreservation.
- Animals, Embryo, Nonmammalian, Freezing.
Abstract
Vitrification could provide a promising tool for the cryopreservation of fish embryos. To achieve successful cryopreservation, several parameters should be taken into account in the design of a vitrification protocol. In the present study, some relevant factors were investigated (choice of a proper vitrificant solutions and temperature for thawing) using neurulation-stage embryos. Six DMSO-based vitrificant solutions (V1-V6) were tested using a 6-step incorporation protocol. DMSO-based vitrificant solutions contained DMSO + permeable cryoprotectants + nonpermeable cryoprotectants. Embryos were immersed in vitrificant solutions for 7 minutes and directly plunged into liquid nitrogen. After vitrification (-196 °C for 10 minutes), the thawing was performed in a water bath at 0 or 20 °C and then embryos incubated until hatched. Our results demonstrated that some embryos vitrified in 5 of 6 vitrification solutions survived and hatched out, but none survived after vitrification in V2. The highest survival rate (45.45%) was observed in samples frozen with the best vitrificant solution (V6) and thawing combination (20 °C). These results establish that cryopreservation of Persian sturgeon (Acipenser persicus) embryos by DMSO-based vitrificant solutions is possible.
DOI: 10.1016/j.theriogenology.2015.11.012
PubMed: 26768541
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Electronic address: skeivanloo@gau.ac.ir.</nlm:affiliation><country xml:lang="fr">Iran</country><wicri:regionArea>Department of Fishery Sciences, Gorgan University of Agricultural Sciences and Natural Resources, Gorgan</wicri:regionArea></affiliation></author><author><name sortKey="Sudagar, Mohammad" sort="Sudagar, Mohammad" uniqKey="Sudagar M" first="Mohammad" last="Sudagar">Mohammad Sudagar</name><affiliation wicri:level="1"><nlm:affiliation>Department of Fishery Sciences, Gorgan University of Agricultural Sciences and Natural Resources, Gorgan, Iran.</nlm:affiliation><country xml:lang="fr">Iran</country><wicri:regionArea>Department of Fishery Sciences, Gorgan University of Agricultural Sciences and Natural Resources, Gorgan</wicri:regionArea></affiliation></author></analytic><series><title level="j">Theriogenology</title><idno type="eISSN">1879-3231</idno><imprint><date when="2016" type="published">2016</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Animals</term><term>Cell Survival (drug effects)</term><term>Cryopreservation (methods)</term><term>Cryopreservation (veterinary)</term><term>Cryoprotective Agents (pharmacology)</term><term>Dimethyl Sulfoxide (pharmacology)</term><term>Embryo, Nonmammalian</term><term>Embryonic Development (drug effects)</term><term>Fishes (embryology)</term><term>Freezing</term><term>Vitrification (drug effects)</term></keywords><keywords scheme="MESH" type="chemical" qualifier="pharmacology" xml:lang="en"><term>Cryoprotective Agents</term><term>Dimethyl Sulfoxide</term></keywords><keywords scheme="MESH" qualifier="drug effects" xml:lang="en"><term>Cell Survival</term><term>Embryonic Development</term><term>Vitrification</term></keywords><keywords scheme="MESH" qualifier="embryology" xml:lang="en"><term>Fishes</term></keywords><keywords scheme="MESH" qualifier="methods" xml:lang="en"><term>Cryopreservation</term></keywords><keywords scheme="MESH" qualifier="veterinary" xml:lang="en"><term>Cryopreservation</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Animals</term><term>Embryo, Nonmammalian</term><term>Freezing</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Vitrification could provide a promising tool for the cryopreservation of fish embryos. To achieve successful cryopreservation, several parameters should be taken into account in the design of a vitrification protocol. In the present study, some relevant factors were investigated (choice of a proper vitrificant solutions and temperature for thawing) using neurulation-stage embryos. Six DMSO-based vitrificant solutions (V1-V6) were tested using a 6-step incorporation protocol. DMSO-based vitrificant solutions contained DMSO + permeable cryoprotectants + nonpermeable cryoprotectants. Embryos were immersed in vitrificant solutions for 7 minutes and directly plunged into liquid nitrogen. After vitrification (-196 °C for 10 minutes), the thawing was performed in a water bath at 0 or 20 °C and then embryos incubated until hatched. Our results demonstrated that some embryos vitrified in 5 of 6 vitrification solutions survived and hatched out, but none survived after vitrification in V2. The highest survival rate (45.45%) was observed in samples frozen with the best vitrificant solution (V6) and thawing combination (20 °C). These results establish that cryopreservation of Persian sturgeon (Acipenser persicus) embryos by DMSO-based vitrificant solutions is possible.</div></front></TEI><pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">26768541</PMID><DateCreated><Year>2016</Year><Month>02</Month><Day>06</Day></DateCreated><DateCompleted><Year>2016</Year><Month>12</Month><Day>13</Day></DateCompleted><DateRevised><Year>2016</Year><Month>12</Month><Day>30</Day></DateRevised><Article PubModel="Print-Electronic"><Journal><ISSN IssnType="Electronic">1879-3231</ISSN><JournalIssue CitedMedium="Internet"><Volume>85</Volume><Issue>5</Issue><PubDate><Year>2016</Year><Month>Mar</Month><Day>15</Day></PubDate></JournalIssue><Title>Theriogenology</Title><ISOAbbreviation>Theriogenology</ISOAbbreviation></Journal><ArticleTitle>Cryopreservation of Persian sturgeon (Acipenser persicus) embryos by DMSO-based vitrificant solutions.</ArticleTitle><Pagination><MedlinePgn>1013-8</MedlinePgn></Pagination><ELocationID EIdType="doi" ValidYN="Y">10.1016/j.theriogenology.2015.11.012</ELocationID><ELocationID EIdType="pii" ValidYN="Y">S0093-691X(15)00632-9</ELocationID><Abstract><AbstractText>Vitrification could provide a promising tool for the cryopreservation of fish embryos. To achieve successful cryopreservation, several parameters should be taken into account in the design of a vitrification protocol. In the present study, some relevant factors were investigated (choice of a proper vitrificant solutions and temperature for thawing) using neurulation-stage embryos. Six DMSO-based vitrificant solutions (V1-V6) were tested using a 6-step incorporation protocol. DMSO-based vitrificant solutions contained DMSO + permeable cryoprotectants + nonpermeable cryoprotectants. Embryos were immersed in vitrificant solutions for 7 minutes and directly plunged into liquid nitrogen. After vitrification (-196 °C for 10 minutes), the thawing was performed in a water bath at 0 or 20 °C and then embryos incubated until hatched. Our results demonstrated that some embryos vitrified in 5 of 6 vitrification solutions survived and hatched out, but none survived after vitrification in V2. The highest survival rate (45.45%) was observed in samples frozen with the best vitrificant solution (V6) and thawing combination (20 °C). These results establish that cryopreservation of Persian sturgeon (Acipenser persicus) embryos by DMSO-based vitrificant solutions is possible.</AbstractText><CopyrightInformation>Copyright © 2016 Elsevier Inc. All rights reserved.</CopyrightInformation></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Keivanloo</LastName><ForeName>Saeide</ForeName><Initials>S</Initials><AffiliationInfo><Affiliation>Department of Fishery Sciences, Gorgan University of Agricultural Sciences and Natural Resources, Gorgan, Iran. 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