Cryopreservation of fish sperm: applications and perspectives
Identifieur interne : 001426 ( Istex/Corpus ); précédent : 001425; suivant : 001427Cryopreservation of fish sperm: applications and perspectives
Auteurs : E. Cabrita ; C. Sarasquete ; S. Martínez-Páramo ; V. Robles ; J. Beirão ; S. Pérez-Cerezales ; M. P. HerráezSource :
- Journal of Applied Ichthyology [ 0175-8659 ] ; 2010-10.
Abstract
Cryopreservation is of interest not only for fish farming but also for the conservation and genetic improvement of resources. This technique has been well established in some freshwater fish species mainly, salmonid, sturgeons and carps, however, only in the last decade research was focused in marine fish species. The benefits of sperm cryopreservation include: (i) synchronization of gamete availability of both sexes, (ii) sperm economy; (iii) simplification of broodstock management, (iv) transport of gametes from different fish farms, and (v) germplasm storage for genetic selection programs or conservation of species. These issues would certainly benefit the aquaculture industry. The tremendous impact that biotechnology is having in aquaculture has been particularly obvious in recent years. Several species are being used as research models not only for aquaculture development applications but also for medical research. Sperm cryopreservation can give an important contribution in the germ storage of all transgenic lines. However, in all applications in fish sperm, cryopreservation needs to overcome a lack in standardization of methodologies and procedures, a correct assay of seminal quality and the development of tools to characterize cryoinjury. Many efforts have recently been made in the study of DNA using different approaches such as the comet assay (single cell gel electrophoresis), TUNEL (terminal deoxynucleotidyl transferase‐nick‐end‐labelling), SCSA (sperm chromatin structure assay) and the analysis of specific DNA sequences using RT‐PCR, since DNA damage may impair fertility or embryo development. Cryopreservation of gametes would certainly benefit from a higher concern on male improvement, basically through nutrition or selection of resistant stocks (e.g. stress resistant individuals or highly adapted to captivity) producing gametes of higher quality. There is a huge window of opportunities for improve the resistance of cells to cryopreservation through diet supplementation of certain compounds such as amino acids (taurine and hypotaurine), vitamins (Vit. E and C) and lipids or through a direct supplementation of the extender media. An equilibrium of those compounds will improve spermatozoa and seminal plasma composition protecting cells against oxidative stress (lipid peroxidation, protein oxidation, DNA fragmentation, enzyme protection) that is gaining each day more importance in cryodamage research.
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DOI: 10.1111/j.1439-0426.2010.01556.x
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Cabrita</name><affiliation><mods:affiliation>Institute of Marine Sciences of Andalusia‐ ICMAN, Spanish National Research Council‐CSIC, Puerto Real, Cádiz, Spain</mods:affiliation></affiliation></author><author><name sortKey="Sarasquete, C" sort="Sarasquete, C" uniqKey="Sarasquete C" first="C." last="Sarasquete">C. Sarasquete</name><affiliation><mods:affiliation>Institute of Marine Sciences of Andalusia‐ ICMAN, Spanish National Research Council‐CSIC, Puerto Real, Cádiz, Spain</mods:affiliation></affiliation></author><author><name sortKey="Martinez Ramo, S" sort="Martinez Ramo, S" uniqKey="Martinez Ramo S" first="S." last="Martínez-Páramo">S. Martínez-Páramo</name><affiliation><mods:affiliation>Center for Marine Sciences‐CCMAR, University of Algarve, Campus Gambelas, Faro, Portugal</mods:affiliation></affiliation></author><author><name sortKey="Robles, V" sort="Robles, V" uniqKey="Robles V" first="V." last="Robles">V. Robles</name><affiliation><mods:affiliation>INDEGSAL and Department Molecular Biology, University of León, León, Spain</mods:affiliation></affiliation></author><author><name sortKey="Beirao, J" sort="Beirao, J" uniqKey="Beirao J" first="J." last="Beirão">J. Beirão</name><affiliation><mods:affiliation>INDEGSAL and Department Molecular Biology, University of León, León, Spain</mods:affiliation></affiliation></author><author><name sortKey="Perez Erezales, S" sort="Perez Erezales, S" uniqKey="Perez Erezales S" first="S." last="Pérez-Cerezales">S. Pérez-Cerezales</name><affiliation><mods:affiliation>INDEGSAL and Department Molecular Biology, University of León, León, Spain</mods:affiliation></affiliation></author><author><name sortKey="Herraez, M P" sort="Herraez, M P" uniqKey="Herraez M" first="M. P." last="Herráez">M. P. Herráez</name><affiliation><mods:affiliation>INDEGSAL and Department Molecular Biology, University of León, León, Spain</mods:affiliation></affiliation></author></analytic><monogr></monogr><series><title level="j">Journal of Applied Ichthyology</title><idno type="ISSN">0175-8659</idno><idno type="eISSN">1439-0426</idno><imprint><publisher>Blackwell Publishing Ltd</publisher><pubPlace>Oxford, UK</pubPlace><date type="published" when="2010-10">2010-10</date><biblScope unit="volume">26</biblScope><biblScope unit="issue">5</biblScope><biblScope unit="page" from="623">623</biblScope><biblScope unit="page" to="635">635</biblScope></imprint><idno type="ISSN">0175-8659</idno></series><idno type="istex">D1ECB1B41B856761A745161BE22B5234CA391771</idno><idno type="DOI">10.1111/j.1439-0426.2010.01556.x</idno><idno type="ArticleID">JAI1556</idno></biblStruct></sourceDesc><seriesStmt><idno type="ISSN">0175-8659</idno></seriesStmt></fileDesc><profileDesc><textClass></textClass><langUsage><language ident="en">en</language></langUsage></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Cryopreservation is of interest not only for fish farming but also for the conservation and genetic improvement of resources. This technique has been well established in some freshwater fish species mainly, salmonid, sturgeons and carps, however, only in the last decade research was focused in marine fish species. The benefits of sperm cryopreservation include: (i) synchronization of gamete availability of both sexes, (ii) sperm economy; (iii) simplification of broodstock management, (iv) transport of gametes from different fish farms, and (v) germplasm storage for genetic selection programs or conservation of species. These issues would certainly benefit the aquaculture industry. The tremendous impact that biotechnology is having in aquaculture has been particularly obvious in recent years. Several species are being used as research models not only for aquaculture development applications but also for medical research. Sperm cryopreservation can give an important contribution in the germ storage of all transgenic lines. However, in all applications in fish sperm, cryopreservation needs to overcome a lack in standardization of methodologies and procedures, a correct assay of seminal quality and the development of tools to characterize cryoinjury. Many efforts have recently been made in the study of DNA using different approaches such as the comet assay (single cell gel electrophoresis), TUNEL (terminal deoxynucleotidyl transferase‐nick‐end‐labelling), SCSA (sperm chromatin structure assay) and the analysis of specific DNA sequences using RT‐PCR, since DNA damage may impair fertility or embryo development. Cryopreservation of gametes would certainly benefit from a higher concern on male improvement, basically through nutrition or selection of resistant stocks (e.g. stress resistant individuals or highly adapted to captivity) producing gametes of higher quality. There is a huge window of opportunities for improve the resistance of cells to cryopreservation through diet supplementation of certain compounds such as amino acids (taurine and hypotaurine), vitamins (Vit. E and C) and lipids or through a direct supplementation of the extender media. An equilibrium of those compounds will improve spermatozoa and seminal plasma composition protecting cells against oxidative stress (lipid peroxidation, protein oxidation, DNA fragmentation, enzyme protection) that is gaining each day more importance in cryodamage research.</div></front></TEI><istex><corpusName>wiley</corpusName><author><json:item><name>E. Cabrita</name><affiliations><json:string>Institute of Marine Sciences of Andalusia‐ ICMAN, Spanish National Research Council‐CSIC, Puerto Real, Cádiz, Spain</json:string></affiliations></json:item><json:item><name>C. 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These issues would certainly benefit the aquaculture industry. The tremendous impact that biotechnology is having in aquaculture has been particularly obvious in recent years. Several species are being used as research models not only for aquaculture development applications but also for medical research. Sperm cryopreservation can give an important contribution in the germ storage of all transgenic lines. However, in all applications in fish sperm, cryopreservation needs to overcome a lack in standardization of methodologies and procedures, a correct assay of seminal quality and the development of tools to characterize cryoinjury. Many efforts have recently been made in the study of DNA using different approaches such as the comet assay (single cell gel electrophoresis), TUNEL (terminal deoxynucleotidyl transferase‐nick‐end‐labelling), SCSA (sperm chromatin structure assay) and the analysis of specific DNA sequences using RT‐PCR, since DNA damage may impair fertility or embryo development. Cryopreservation of gametes would certainly benefit from a higher concern on male improvement, basically through nutrition or selection of resistant stocks (e.g. stress resistant individuals or highly adapted to captivity) producing gametes of higher quality. There is a huge window of opportunities for improve the resistance of cells to cryopreservation through diet supplementation of certain compounds such as amino acids (taurine and hypotaurine), vitamins (Vit. E and C) and lipids or through a direct supplementation of the extender media. 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type="first">S.</forename><surname>Martínez‐Páramo</surname></persName><affiliation>Center for Marine Sciences‐CCMAR, University of Algarve, Campus Gambelas, Faro, Portugal</affiliation></author><author xml:id="author-4"><persName><forename type="first">V.</forename><surname>Robles</surname></persName><affiliation>INDEGSAL and Department Molecular Biology, University of León, León, Spain</affiliation></author><author xml:id="author-5"><persName><forename type="first">J.</forename><surname>Beirão</surname></persName><affiliation>INDEGSAL and Department Molecular Biology, University of León, León, Spain</affiliation></author><author xml:id="author-6"><persName><forename type="first">S.</forename><surname>Pérez‐Cerezales</surname></persName><affiliation>INDEGSAL and Department Molecular Biology, University of León, León, Spain</affiliation></author><author xml:id="author-7"><persName><forename type="first">M.P.</forename><surname>Herráez</surname></persName><affiliation>INDEGSAL and Department Molecular Biology, University of León, León, Spain</affiliation></author></analytic><monogr><title level="j">Journal of Applied Ichthyology</title><idno type="pISSN">0175-8659</idno><idno type="eISSN">1439-0426</idno><idno type="DOI">10.1111/(ISSN)1439-0426</idno><imprint><publisher>Blackwell Publishing Ltd</publisher><pubPlace>Oxford, UK</pubPlace><date type="published" when="2010-10"></date><biblScope unit="volume">26</biblScope><biblScope unit="issue">5</biblScope><biblScope unit="page" from="623">623</biblScope><biblScope unit="page" to="635">635</biblScope></imprint></monogr><idno type="istex">D1ECB1B41B856761A745161BE22B5234CA391771</idno><idno type="DOI">10.1111/j.1439-0426.2010.01556.x</idno><idno type="ArticleID">JAI1556</idno></biblStruct></sourceDesc></fileDesc><profileDesc><creation><date>2010</date></creation><langUsage><language ident="en">en</language></langUsage><abstract xml:lang="en"><p>Cryopreservation is of interest not only for fish farming but also for the conservation and genetic improvement of resources. This technique has been well established in some freshwater fish species mainly, salmonid, sturgeons and carps, however, only in the last decade research was focused in marine fish species. The benefits of sperm cryopreservation include: (i) synchronization of gamete availability of both sexes, (ii) sperm economy; (iii) simplification of broodstock management, (iv) transport of gametes from different fish farms, and (v) germplasm storage for genetic selection programs or conservation of species. These issues would certainly benefit the aquaculture industry. The tremendous impact that biotechnology is having in aquaculture has been particularly obvious in recent years. Several species are being used as research models not only for aquaculture development applications but also for medical research. Sperm cryopreservation can give an important contribution in the germ storage of all transgenic lines. However, in all applications in fish sperm, cryopreservation needs to overcome a lack in standardization of methodologies and procedures, a correct assay of seminal quality and the development of tools to characterize cryoinjury. Many efforts have recently been made in the study of DNA using different approaches such as the comet assay (single cell gel electrophoresis), TUNEL (terminal deoxynucleotidyl transferase‐nick‐end‐labelling), SCSA (sperm chromatin structure assay) and the analysis of specific DNA sequences using RT‐PCR, since DNA damage may impair fertility or embryo development. Cryopreservation of gametes would certainly benefit from a higher concern on male improvement, basically through nutrition or selection of resistant stocks (e.g. stress resistant individuals or highly adapted to captivity) producing gametes of higher quality. There is a huge window of opportunities for improve the resistance of cells to cryopreservation through diet supplementation of certain compounds such as amino acids (taurine and hypotaurine), vitamins (Vit. E and C) and lipids or through a direct supplementation of the extender media. An equilibrium of those compounds will improve spermatozoa and seminal plasma composition protecting cells against oxidative stress (lipid peroxidation, protein oxidation, DNA fragmentation, enzyme protection) that is gaining each day more importance in cryodamage research.</p></abstract></profileDesc><revisionDesc><change when="2010-10">Published</change></revisionDesc></teiHeader></istex:fulltextTEI><json:item><extension>txt</extension><original>false</original><mimetype>text/plain</mimetype><uri>https://api.istex.fr/document/D1ECB1B41B856761A745161BE22B5234CA391771/fulltext/txt</uri></json:item></fulltext><metadata><istex:metadataXml wicri:clean="Wiley, elements deleted: body"><istex:xmlDeclaration>version="1.0" encoding="UTF-8" standalone="yes"</istex:xmlDeclaration><istex:document><component version="2.0" type="serialArticle" xml:lang="en"><header><publicationMeta level="product"><publisherInfo><publisherName>Blackwell Publishing Ltd</publisherName><publisherLoc>Oxford, UK</publisherLoc></publisherInfo><doi origin="wiley" registered="yes">10.1111/(ISSN)1439-0426</doi><issn type="print">0175-8659</issn><issn type="electronic">1439-0426</issn><idGroup><id type="product" value="JAI"></id><id type="publisherDivision" value="ST"></id></idGroup><titleGroup><title type="main" sort="JOURNAL OF APPLIED ICHTHYOLOGY">Journal of Applied Ichthyology</title></titleGroup></publicationMeta><publicationMeta level="part" position="10105"><doi origin="wiley">10.1111/jai.2010.26.issue-5</doi><titleGroup><title type="specialIssueTitle">Proceedings of the Second International Workshop on Biology of Fish Gametes
Special Issue Editors: Harald Rosenthal, Juan F. Asturiano, Otomar Linhart, Akos Horvath</title></titleGroup><numberingGroup><numbering type="journalVolume" number="26">26</numbering><numbering type="journalIssue">5</numbering></numberingGroup><coverDate startDate="2010-10">October 2010</coverDate></publicationMeta><publicationMeta level="unit" type="reviewArticle" position="2" status="forIssue"><doi origin="wiley">10.1111/j.1439-0426.2010.01556.x</doi><idGroup><id type="unit" value="JAI1556"></id></idGroup><countGroup><count type="pageTotal" number="13"></count></countGroup><titleGroup><title type="tocHeading1">Review article</title></titleGroup><copyright>© 2010 Blackwell Verlag, Berlin</copyright><eventGroup><event type="xmlConverted" agent="Converter:BPG_TO_WML3G version:2.3.19 mode:FullText" date="2010-09-17"></event><event type="firstOnline" date="2010-09-17"></event><event type="publishedOnlineFinalForm" date="2010-09-17"></event><event type="xmlConverted" agent="Converter:WILEY_ML3G_TO_WILEY_ML3GV2 version:3.8.8" date="2014-01-28"></event><event type="xmlConverted" agent="Converter:WML3G_To_WML3G version:4.1.7 mode:FullText,remove_FC" date="2014-10-23"></event></eventGroup><numberingGroup><numbering type="pageFirst" number="623">623</numbering><numbering type="pageLast" number="635">635</numbering></numberingGroup><correspondenceTo><b>Author’s address:</b> Elsa Cabrita, Institute of Marine Sciences of Andalusia‐ ICMAN, Spanish National Research Council‐CSIC, AV. Republica Saharaui 2, 11510 Puerto Real, Cádiz, Spain.
E‐mail: <email>elsa.cabrita@icman.csic.es</email></correspondenceTo><linkGroup><link type="toTypesetVersion" href="file:JAI.JAI1556.pdf"></link></linkGroup></publicationMeta><contentMeta><unparsedEditorialHistory>Received: March 14, 2010 Accepted: July 30, 2010</unparsedEditorialHistory><countGroup><count type="figureTotal" number="0"></count><count type="tableTotal" number="2"></count></countGroup><titleGroup><title type="main">Cryopreservation of fish sperm: applications and perspectives</title><title type="shortAuthors">E. Cabrita et al.</title><title type="short">Cryopreservation of fish sperm</title></titleGroup><creators><creator creatorRole="author" xml:id="cr1" affiliationRef="#a1"><personName><givenNames>E.</givenNames><familyName>Cabrita</familyName></personName></creator><creator creatorRole="author" xml:id="cr2" affiliationRef="#a1"><personName><givenNames>C.</givenNames><familyName>Sarasquete</familyName></personName></creator><creator creatorRole="author" xml:id="cr3" affiliationRef="#a2"><personName><givenNames>S.</givenNames><familyName>Martínez‐Páramo</familyName></personName></creator><creator creatorRole="author" xml:id="cr4" affiliationRef="#a3"><personName><givenNames>V.</givenNames><familyName>Robles</familyName></personName></creator><creator creatorRole="author" xml:id="cr5" affiliationRef="#a3"><personName><givenNames>J.</givenNames><familyName>Beirão</familyName></personName></creator><creator creatorRole="author" xml:id="cr6" affiliationRef="#a3"><personName><givenNames>S.</givenNames><familyName>Pérez‐Cerezales</familyName></personName></creator><creator creatorRole="author" xml:id="cr7" affiliationRef="#a3"><personName><givenNames>M.P.</givenNames><familyName>Herráez</familyName></personName></creator></creators><affiliationGroup><affiliation xml:id="a1" countryCode="ES"><unparsedAffiliation>Institute of Marine Sciences of Andalusia‐ ICMAN, Spanish National Research Council‐CSIC, Puerto Real, Cádiz, Spain</unparsedAffiliation></affiliation><affiliation xml:id="a2" countryCode="PT"><unparsedAffiliation>Center for Marine Sciences‐CCMAR, University of Algarve, Campus Gambelas, Faro, Portugal</unparsedAffiliation></affiliation><affiliation xml:id="a3" countryCode="ES"><unparsedAffiliation>INDEGSAL and Department Molecular Biology, University of León, León, Spain</unparsedAffiliation></affiliation></affiliationGroup><abstractGroup><abstract type="main" xml:lang="en"><title type="main">Summary</title><p>Cryopreservation is of interest not only for fish farming but also for the conservation and genetic improvement of resources. This technique has been well established in some freshwater fish species mainly, salmonid, sturgeons and carps, however, only in the last decade research was focused in marine fish species. The benefits of sperm cryopreservation include: (i) synchronization of gamete availability of both sexes, (ii) sperm economy; (iii) simplification of broodstock management, (iv) transport of gametes from different fish farms, and (v) germplasm storage for genetic selection programs or conservation of species. These issues would certainly benefit the aquaculture industry. The tremendous impact that biotechnology is having in aquaculture has been particularly obvious in recent years. Several species are being used as research models not only for aquaculture development applications but also for medical research. Sperm cryopreservation can give an important contribution in the germ storage of all transgenic lines. However, in all applications in fish sperm, cryopreservation needs to overcome a lack in standardization of methodologies and procedures, a correct assay of seminal quality and the development of tools to characterize cryoinjury. Many efforts have recently been made in the study of DNA using different approaches such as the comet assay (single cell gel electrophoresis), TUNEL (terminal deoxynucleotidyl transferase‐nick‐end‐labelling), SCSA (sperm chromatin structure assay) and the analysis of specific DNA sequences using RT‐PCR, since DNA damage may impair fertility or embryo development. Cryopreservation of gametes would certainly benefit from a higher concern on male improvement, basically through nutrition or selection of resistant stocks (e.g. stress resistant individuals or highly adapted to captivity) producing gametes of higher quality. There is a huge window of opportunities for improve the resistance of cells to cryopreservation through diet supplementation of certain compounds such as amino acids (taurine and hypotaurine), vitamins (Vit. E and C) and lipids or through a direct supplementation of the extender media. An equilibrium of those compounds will improve spermatozoa and seminal plasma composition protecting cells against oxidative stress (lipid peroxidation, protein oxidation, DNA fragmentation, enzyme protection) that is gaining each day more importance in cryodamage research.</p></abstract></abstractGroup></contentMeta></header></component></istex:document></istex:metadataXml><mods version="3.6"><titleInfo lang="en"><title>Cryopreservation of fish sperm: applications and perspectives</title></titleInfo><titleInfo type="abbreviated" lang="en"><title>Cryopreservation of fish sperm</title></titleInfo><titleInfo type="alternative" contentType="CDATA" lang="en"><title>Cryopreservation of fish sperm: applications and perspectives</title></titleInfo><name type="personal"><namePart type="given">E.</namePart><namePart type="family">Cabrita</namePart><affiliation>Institute of Marine Sciences of Andalusia‐ ICMAN, Spanish National Research Council‐CSIC, Puerto Real, Cádiz, Spain</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">C.</namePart><namePart type="family">Sarasquete</namePart><affiliation>Institute of Marine Sciences of Andalusia‐ ICMAN, Spanish National Research Council‐CSIC, Puerto Real, Cádiz, Spain</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">S.</namePart><namePart type="family">Martínez‐Páramo</namePart><affiliation>Center for Marine Sciences‐CCMAR, University of Algarve, Campus Gambelas, Faro, Portugal</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">V.</namePart><namePart type="family">Robles</namePart><affiliation>INDEGSAL and Department Molecular Biology, University of León, León, Spain</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">J.</namePart><namePart type="family">Beirão</namePart><affiliation>INDEGSAL and Department Molecular Biology, University of León, León, Spain</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">S.</namePart><namePart type="family">Pérez‐Cerezales</namePart><affiliation>INDEGSAL and Department Molecular Biology, University of León, León, Spain</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">M.P.</namePart><namePart type="family">Herráez</namePart><affiliation>INDEGSAL and Department Molecular Biology, University of León, León, Spain</affiliation><role><roleTerm type="text">author</roleTerm></role></name><typeOfResource>text</typeOfResource><genre type="review-article" displayLabel="reviewArticle"></genre><originInfo><publisher>Blackwell Publishing Ltd</publisher><place><placeTerm type="text">Oxford, UK</placeTerm></place><dateIssued encoding="w3cdtf">2010-10</dateIssued><edition>Received: March 14, 2010 Accepted: July 30, 2010</edition><copyrightDate encoding="w3cdtf">2010</copyrightDate></originInfo><language><languageTerm type="code" authority="rfc3066">en</languageTerm><languageTerm type="code" authority="iso639-2b">eng</languageTerm></language><physicalDescription><internetMediaType>text/html</internetMediaType><extent unit="tables">2</extent></physicalDescription><abstract lang="en">Cryopreservation is of interest not only for fish farming but also for the conservation and genetic improvement of resources. This technique has been well established in some freshwater fish species mainly, salmonid, sturgeons and carps, however, only in the last decade research was focused in marine fish species. The benefits of sperm cryopreservation include: (i) synchronization of gamete availability of both sexes, (ii) sperm economy; (iii) simplification of broodstock management, (iv) transport of gametes from different fish farms, and (v) germplasm storage for genetic selection programs or conservation of species. These issues would certainly benefit the aquaculture industry. The tremendous impact that biotechnology is having in aquaculture has been particularly obvious in recent years. Several species are being used as research models not only for aquaculture development applications but also for medical research. Sperm cryopreservation can give an important contribution in the germ storage of all transgenic lines. However, in all applications in fish sperm, cryopreservation needs to overcome a lack in standardization of methodologies and procedures, a correct assay of seminal quality and the development of tools to characterize cryoinjury. Many efforts have recently been made in the study of DNA using different approaches such as the comet assay (single cell gel electrophoresis), TUNEL (terminal deoxynucleotidyl transferase‐nick‐end‐labelling), SCSA (sperm chromatin structure assay) and the analysis of specific DNA sequences using RT‐PCR, since DNA damage may impair fertility or embryo development. Cryopreservation of gametes would certainly benefit from a higher concern on male improvement, basically through nutrition or selection of resistant stocks (e.g. stress resistant individuals or highly adapted to captivity) producing gametes of higher quality. There is a huge window of opportunities for improve the resistance of cells to cryopreservation through diet supplementation of certain compounds such as amino acids (taurine and hypotaurine), vitamins (Vit. E and C) and lipids or through a direct supplementation of the extender media. An equilibrium of those compounds will improve spermatozoa and seminal plasma composition protecting cells against oxidative stress (lipid peroxidation, protein oxidation, DNA fragmentation, enzyme protection) that is gaining each day more importance in cryodamage research.</abstract><relatedItem type="host"><titleInfo><title>Journal of Applied Ichthyology</title></titleInfo><genre type="journal">journal</genre><identifier type="ISSN">0175-8659</identifier><identifier type="eISSN">1439-0426</identifier><identifier type="DOI">10.1111/(ISSN)1439-0426</identifier><identifier type="PublisherID">JAI</identifier><part><date>2010</date><detail type="title"><title>Proceedings of the Second International Workshop on Biology of Fish Gametes Special Issue Editors: Harald Rosenthal, Juan F. Asturiano, Otomar Linhart, Akos Horvath</title></detail><detail type="volume"><caption>vol.</caption><number>26</number></detail><detail type="issue"><caption>no.</caption><number>5</number></detail><extent unit="pages"><start>623</start><end>635</end><total>13</total></extent></part></relatedItem><identifier type="istex">D1ECB1B41B856761A745161BE22B5234CA391771</identifier><identifier type="DOI">10.1111/j.1439-0426.2010.01556.x</identifier><identifier type="ArticleID">JAI1556</identifier><accessCondition type="use and reproduction" contentType="copyright">© 2010 Blackwell Verlag, Berlin</accessCondition><recordInfo><recordContentSource>WILEY</recordContentSource><recordOrigin>Blackwell Publishing Ltd</recordOrigin></recordInfo></mods></metadata><serie></serie></istex></record>Pour manipuler ce document sous Unix (Dilib)
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