Estrogenic and toxic effects of methoxychlor on zebrafish (Danio rerio)
Identifieur interne : 001425 ( Istex/Corpus ); précédent : 001424; suivant : 001426Estrogenic and toxic effects of methoxychlor on zebrafish (Danio rerio)
Auteurs : Bram J. Versonnen ; Patrick Roose ; Els M. Monteyne ; Colin R. JanssenSource :
- Environmental Toxicology and Chemistry [ 0730-7268 ] ; 2004-09.
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Abstract
Although zebrafish (Danio rerio) have been suggested as a good candidate for screening potential endocrine disruptors, little information is available on the effects of weak estrogens on this species. We investigated the sensitivity of different life stages of zebrafish toward toxic and estrogenic properties of methoxychlor (MXC). Short‐term tests with adults resulted in a sex‐specific 96‐h lethal concentration for 50% (LC50) of the test animals of 36 μg/L for males and 129 μg/L for females. To determine the estrogenic capacity of MXC, adult zebrafish were exposed to 0, 0.5, 5, and 50 μg MXC/L for 14 d. Induction of vitellogenin ([VTG] measured with protein electrophoresis and Western blot) in males was detected at 5 and 50 μg MXC/L. Females, however, did not exhibit higher blood VTG concentrations at the tested MXC concentrations. In a second series of experiments, juvenile zebrafish were exposed to 0, 0.05, 0.5, and 5 μg MXC/L for 33 d. Survival, length, weight, and condition of larvae were examined as indicators of toxic stress and the VTG content in whole body homogenates of juveniles was measured to determine xenoestrogenic effects. No effects of the tested concentrations of MXC were observed. Finally, the effect of MXC on zebrafish eggs, exposed to 0, 1, 10, and 32 μg MXC/L, was examined. Hatching and survival of hatched zebrafish were affected at 10 and 32 μg MXC/L. This study demonstrated that adult male zebrafish are sensitive toward the estrogenic effects of MXC. However, the use of VTG induction to detect effects of (xeno)estrogens in early life stages has to be further investigated, as low concentrations of VTG were detectable in exposed as well as unexposed juvenile fish.
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DOI: 10.1897/03-228
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We investigated the sensitivity of different life stages of zebrafish toward toxic and estrogenic properties of methoxychlor (MXC). Short‐term tests with adults resulted in a sex‐specific 96‐h lethal concentration for 50% (LC50) of the test animals of 36 μg/L for males and 129 μg/L for females. To determine the estrogenic capacity of MXC, adult zebrafish were exposed to 0, 0.5, 5, and 50 μg MXC/L for 14 d. Induction of vitellogenin ([VTG] measured with protein electrophoresis and Western blot) in males was detected at 5 and 50 μg MXC/L. Females, however, did not exhibit higher blood VTG concentrations at the tested MXC concentrations. In a second series of experiments, juvenile zebrafish were exposed to 0, 0.05, 0.5, and 5 μg MXC/L for 33 d. Survival, length, weight, and condition of larvae were examined as indicators of toxic stress and the VTG content in whole body homogenates of juveniles was measured to determine xenoestrogenic effects. No effects of the tested concentrations of MXC were observed. Finally, the effect of MXC on zebrafish eggs, exposed to 0, 1, 10, and 32 μg MXC/L, was examined. Hatching and survival of hatched zebrafish were affected at 10 and 32 μg MXC/L. This study demonstrated that adult male zebrafish are sensitive toward the estrogenic effects of MXC. However, the use of VTG induction to detect effects of (xeno)estrogens in early life stages has to be further investigated, as low concentrations of VTG were detectable in exposed as well as unexposed juvenile fish.</div></front></TEI><istex><corpusName>wiley</corpusName><author><json:item><name>Bram J. Versonnen</name><affiliations><json:string>Ghent University, Laboratory of Environmental Toxicology and Aquatic Ecology, J. Plateaustraat 22, B‐9000 Ghent, Belgium</json:string><json:string>Current Address: B. 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No effects of the tested concentrations of MXC were observed. Finally, the effect of MXC on zebrafish eggs, exposed to 0, 1, 10, and 32 μg MXC/L, was examined. Hatching and survival of hatched zebrafish were affected at 10 and 32 μg MXC/L. This study demonstrated that adult male zebrafish are sensitive toward the estrogenic effects of MXC. 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Plateaustraat 22, B‐9000 Ghent, Belgium</unparsedAffiliation></affiliation><affiliation xml:id="af2" countryCode="BE" type="organization"><unparsedAffiliation>Management Unit of the North Sea Mathematical Models, Royal Belgian Institute for Natural Sciences, 3rd and 23rd Linieregimentsplein, B‐8400 Oostende, Belgium</unparsedAffiliation></affiliation><affiliation xml:id="curr1" countryCode="BE"><unparsedAffiliation>B. Versonnen is EURAS, Rijvisschestraat 118/3, B‐9052 Gent, Belgium</unparsedAffiliation></affiliation></affiliationGroup><keywordGroup xml:lang="en" type="author"><keyword xml:id="kwd1">Fish</keyword><keyword xml:id="kwd2">Endocrine disruption</keyword><keyword xml:id="kwd3">Methoxychlor</keyword><keyword xml:id="kwd4">Embryo</keyword><keyword xml:id="kwd5">Toxicity</keyword></keywordGroup><abstractGroup><abstract type="main" xml:lang="en"><title type="main">Abstract</title><p>Although zebrafish (<i>Danio rerio</i>) have been suggested as a good candidate for screening potential endocrine disruptors, little information is available on the effects of weak estrogens on this species. We investigated the sensitivity of different life stages of zebrafish toward toxic and estrogenic properties of methoxychlor (MXC). Short‐term tests with adults resulted in a sex‐specific 96‐h lethal concentration for 50% (LC50) of the test animals of 36 μg/L for males and 129 μg/L for females. To determine the estrogenic capacity of MXC, adult zebrafish were exposed to 0, 0.5, 5, and 50 μg MXC/L for 14 d. Induction of vitellogenin ([VTG] measured with protein electrophoresis and Western blot) in males was detected at 5 and 50 μg MXC/L. Females, however, did not exhibit higher blood VTG concentrations at the tested MXC concentrations. In a second series of experiments, juvenile zebrafish were exposed to 0, 0.05, 0.5, and 5 μg MXC/L for 33 d. Survival, length, weight, and condition of larvae were examined as indicators of toxic stress and the VTG content in whole body homogenates of juveniles was measured to determine xenoestrogenic effects. No effects of the tested concentrations of MXC were observed. Finally, the effect of MXC on zebrafish eggs, exposed to 0, 1, 10, and 32 μg MXC/L, was examined. Hatching and survival of hatched zebrafish were affected at 10 and 32 μg MXC/L. This study demonstrated that adult male zebrafish are sensitive toward the estrogenic effects of MXC. However, the use of VTG induction to detect effects of (xeno)estrogens in early life stages has to be further investigated, as low concentrations of VTG were detectable in exposed as well as unexposed juvenile fish.</p></abstract></abstractGroup></contentMeta></header></component></istex:document></istex:metadataXml><mods version="3.6"><titleInfo lang="en"><title>Estrogenic and toxic effects of methoxychlor on zebrafish (Danio rerio)</title></titleInfo><titleInfo type="abbreviated" lang="en"><title>Effects of methoxychlor on zebrafish</title></titleInfo><titleInfo type="alternative" contentType="CDATA" lang="en"><title>Estrogenic and toxic effects of methoxychlor on zebrafish (Danio rerio)</title></titleInfo><name type="personal"><namePart type="given">Bram J.</namePart><namePart type="family">Versonnen</namePart><affiliation>Ghent University, Laboratory of Environmental Toxicology and Aquatic Ecology, J. Plateaustraat 22, B‐9000 Ghent, Belgium</affiliation><affiliation>Current Address: B. Versonnen is EURAS, Rijvisschestraat 118/3, B‐9052 Gent, Belgium</affiliation><affiliation>Ghent University, Laboratory of Environmental Toxicology and Aquatic Ecology, J. Plateaustraat 22, B‐9000 Ghent, Belgium</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">Patrick</namePart><namePart type="family">Roose</namePart><affiliation>Management Unit of the North Sea Mathematical Models, Royal Belgian Institute for Natural Sciences, 3rd and 23rd Linieregimentsplein, B‐8400 Oostende, Belgium</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">Els M.</namePart><namePart type="family">Monteyne</namePart><affiliation>Management Unit of the North Sea Mathematical Models, Royal Belgian Institute for Natural Sciences, 3rd and 23rd Linieregimentsplein, B‐8400 Oostende, Belgium</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">Colin R.</namePart><namePart type="family">Janssen</namePart><affiliation>Ghent University, Laboratory of Environmental Toxicology and Aquatic Ecology, J. Plateaustraat 22, B‐9000 Ghent, Belgium</affiliation><role><roleTerm type="text">author</roleTerm></role></name><typeOfResource>text</typeOfResource><genre type="article" displayLabel="article"></genre><originInfo><publisher>Wiley Periodicals, Inc.</publisher><place><placeTerm type="text">Hoboken</placeTerm></place><dateIssued encoding="w3cdtf">2004-09</dateIssued><dateCaptured encoding="w3cdtf">2003-04-23</dateCaptured><dateValid encoding="w3cdtf">2004-02-20</dateValid><copyrightDate encoding="w3cdtf">2004</copyrightDate></originInfo><language><languageTerm type="code" authority="rfc3066">en</languageTerm><languageTerm type="code" authority="iso639-2b">eng</languageTerm></language><physicalDescription><internetMediaType>text/html</internetMediaType><extent unit="figures">4</extent><extent unit="tables">3</extent><extent unit="references">46</extent></physicalDescription><abstract lang="en">Although zebrafish (Danio rerio) have been suggested as a good candidate for screening potential endocrine disruptors, little information is available on the effects of weak estrogens on this species. We investigated the sensitivity of different life stages of zebrafish toward toxic and estrogenic properties of methoxychlor (MXC). Short‐term tests with adults resulted in a sex‐specific 96‐h lethal concentration for 50% (LC50) of the test animals of 36 μg/L for males and 129 μg/L for females. To determine the estrogenic capacity of MXC, adult zebrafish were exposed to 0, 0.5, 5, and 50 μg MXC/L for 14 d. Induction of vitellogenin ([VTG] measured with protein electrophoresis and Western blot) in males was detected at 5 and 50 μg MXC/L. Females, however, did not exhibit higher blood VTG concentrations at the tested MXC concentrations. In a second series of experiments, juvenile zebrafish were exposed to 0, 0.05, 0.5, and 5 μg MXC/L for 33 d. Survival, length, weight, and condition of larvae were examined as indicators of toxic stress and the VTG content in whole body homogenates of juveniles was measured to determine xenoestrogenic effects. No effects of the tested concentrations of MXC were observed. Finally, the effect of MXC on zebrafish eggs, exposed to 0, 1, 10, and 32 μg MXC/L, was examined. Hatching and survival of hatched zebrafish were affected at 10 and 32 μg MXC/L. This study demonstrated that adult male zebrafish are sensitive toward the estrogenic effects of MXC. However, the use of VTG induction to detect effects of (xeno)estrogens in early life stages has to be further investigated, as low concentrations of VTG were detectable in exposed as well as unexposed juvenile fish.</abstract><subject lang="en"><genre>keywords</genre><topic>Fish</topic><topic>Endocrine disruption</topic><topic>Methoxychlor</topic><topic>Embryo</topic><topic>Toxicity</topic></subject><relatedItem type="host"><titleInfo><title>Environmental Toxicology and Chemistry</title><subTitle>An International Journal</subTitle></titleInfo><titleInfo type="abbreviated"><title>Environmental Toxicology and Chemistry</title></titleInfo><genre type="journal">journal</genre><subject><genre>article-category</genre><topic>Environmental Toxicology</topic></subject><identifier type="ISSN">0730-7268</identifier><identifier type="eISSN">1552-8618</identifier><identifier type="DOI">10.1002/(ISSN)1552-8618</identifier><identifier type="PublisherID">ETC</identifier><part><date>2004</date><detail type="volume"><caption>vol.</caption><number>23</number></detail><detail type="issue"><caption>no.</caption><number>9</number></detail><extent unit="pages"><start>2194</start><end>2201</end><total>8</total></extent></part></relatedItem><identifier type="istex">0E80FF278D0DF17A0E73803D9BFEA6DA79A01120</identifier><identifier type="DOI">10.1897/03-228</identifier><identifier type="ArticleID">ETC5620230920</identifier><accessCondition type="use and reproduction" contentType="copyright">Copyright © 2004 SETAC</accessCondition><recordInfo><recordContentSource>WILEY</recordContentSource><recordOrigin>Wiley Periodicals, Inc.</recordOrigin></recordInfo></mods></metadata><serie></serie></istex></record>Pour manipuler ce document sous Unix (Dilib)
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