Urotensin II in the central nervous system of the frog Rana ridibunda: immunohistochemical localization and biochemical characterization
Identifieur interne : 001414 ( Istex/Corpus ); précédent : 001413; suivant : 001415Urotensin II in the central nervous system of the frog Rana ridibunda: immunohistochemical localization and biochemical characterization
Auteurs : Nicolas Chartrel ; J. Michael Conlon ; Françoise Collin ; Benedicte Braun ; David Waugh ; Mauro Vallarino ; Sabine L. Lahrichi ; Jean E. Rivier ; Hubert VaudrySource :
- Journal of Comparative Neurology [ 0021-9967 ] ; 1996-01-08.
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- KwdEn :
Abstract
Urotensin II (UII) is traditionally regarded as a product of the neurosecretory cells in the caudal portion of the spinal cord of jawed fishes. A peptide related to UII has been recently isolated from the frog brain, thereby providing the first evidence that UII is also present in the central nervous system of a tetrapod. In the present study, we have investigated the distribution of UII‐immunoreactive elements in the brain and spinal cord of the frog Rana ridibunda by immunofluorescence using an antiserum directed against the conserved cyclic region of the peptide. Two distinct populations of UII‐immunoreactive perikarya were visualized. The first group of positive neurons was found in the nucleus hypoglossus of the medulla oblongata, which controls two striated muscles of the tongue. The second population of immunoreactive cell bodies was represented by a subset of motoneurons that were particularly abundant in the caudal region of the cord (34% of the motoneuron population). The telencephalon, diencephalon, mesencephalon, and metencephalon were totally devoid of UII‐containing cell bodies but displayed dense networks of UII‐immunoreactive fibers, notably in the thalamus, the tectum, the tegmentum, and the granular layer of the cerebellum. In addition, a dense bundle of long varicose processes projecting rostrocaudally was observed coursing along the ventral surface of the brain from the midtelencephalon to the medulla oblongata. Reversed‐phase high‐performance liquid chromatography analysis of frog brain, medulla oblongata, and spinal cord extracts revealed that, in all three regions, UII‐immunoreactive material eluted as a single peak which exhibited the same retention time as synthetic frog UII. Taken together, these data indicate that UII, in addition to its neuroendocrine functions in fish, is a potential regulatory peptide in the central nervous system of amphibians. © 1996 Wiley‐Liss, Inc.
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DOI: 10.1002/(SICI)1096-9861(19960108)364:2<324::AID-CNE10>3.0.CO;2-P
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Michael Conlon</name><affiliation><mods:affiliation>Regulatory Peptide Center, Department of Biomedical Sciences, Creighton University Medical School, Omaha, Nebraska 68178</mods:affiliation></affiliation></author><author><name sortKey="Collin, Francoise" sort="Collin, Francoise" uniqKey="Collin F" first="Françoise" last="Collin">Françoise Collin</name><affiliation><mods:affiliation>European Institute for Peptide Research, Laboratory of Cellular and Molecular Neuroendocrinology, INSERM U 413, UA CNRS, University of Rouen, 76821 Mont‐Saint‐Aignan, France</mods:affiliation></affiliation></author><author><name sortKey="Braun, Benedicte" sort="Braun, Benedicte" uniqKey="Braun B" first="Benedicte" last="Braun">Benedicte Braun</name><affiliation><mods:affiliation>European Institute for Peptide Research, Laboratory of Cellular and Molecular Neuroendocrinology, INSERM U 413, UA CNRS, University of Rouen, 76821 Mont‐Saint‐Aignan, France</mods:affiliation></affiliation></author><author><name sortKey="Waugh, David" sort="Waugh, David" uniqKey="Waugh D" first="David" last="Waugh">David Waugh</name><affiliation><mods:affiliation>Regulatory Peptide Center, Department of Biomedical Sciences, Creighton University Medical School, Omaha, Nebraska 68178</mods:affiliation></affiliation></author><author><name sortKey="Vallarino, Mauro" sort="Vallarino, Mauro" uniqKey="Vallarino M" first="Mauro" last="Vallarino">Mauro Vallarino</name><affiliation><mods:affiliation>Institute of Comparative Anatomy, University of Genova, 16132 Genova, Italy</mods:affiliation></affiliation></author><author><name sortKey="Lahrichi, Sabine L" sort="Lahrichi, Sabine L" uniqKey="Lahrichi S" first="Sabine L." last="Lahrichi">Sabine L. 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Rivier</name><affiliation><mods:affiliation>Clayton Foundation Laboratories for Peptide Biology, Salk Institute for Biological Studies, La Jolla, California 92037</mods:affiliation></affiliation></author><author><name sortKey="Vaudry, Hubert" sort="Vaudry, Hubert" uniqKey="Vaudry H" first="Hubert" last="Vaudry">Hubert Vaudry</name><affiliation><mods:affiliation>European Institute for Peptide Research, Laboratory of Cellular and Molecular Neuroendocrinology, INSERM U 413, UA CNRS, University of Rouen, 76821 Mont‐Saint‐Aignan, France</mods:affiliation></affiliation><affiliation><mods:affiliation>European Institute for Peptide Research, Laboratory of Cellular and Molecular Neuroendocrinology, INSERM U 413, UA CNRS, University of Rouen, 76821 Mont‐Saint Aignan, France</mods:affiliation></affiliation></author></analytic><monogr></monogr><series><title level="j">Journal of Comparative Neurology</title><title level="j" type="abbrev">J. Comp. 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A peptide related to UII has been recently isolated from the frog brain, thereby providing the first evidence that UII is also present in the central nervous system of a tetrapod. In the present study, we have investigated the distribution of UII‐immunoreactive elements in the brain and spinal cord of the frog Rana ridibunda by immunofluorescence using an antiserum directed against the conserved cyclic region of the peptide. Two distinct populations of UII‐immunoreactive perikarya were visualized. The first group of positive neurons was found in the nucleus hypoglossus of the medulla oblongata, which controls two striated muscles of the tongue. The second population of immunoreactive cell bodies was represented by a subset of motoneurons that were particularly abundant in the caudal region of the cord (34% of the motoneuron population). The telencephalon, diencephalon, mesencephalon, and metencephalon were totally devoid of UII‐containing cell bodies but displayed dense networks of UII‐immunoreactive fibers, notably in the thalamus, the tectum, the tegmentum, and the granular layer of the cerebellum. In addition, a dense bundle of long varicose processes projecting rostrocaudally was observed coursing along the ventral surface of the brain from the midtelencephalon to the medulla oblongata. Reversed‐phase high‐performance liquid chromatography analysis of frog brain, medulla oblongata, and spinal cord extracts revealed that, in all three regions, UII‐immunoreactive material eluted as a single peak which exhibited the same retention time as synthetic frog UII. Taken together, these data indicate that UII, in addition to its neuroendocrine functions in fish, is a potential regulatory peptide in the central nervous system of amphibians. © 1996 Wiley‐Liss, Inc.</div></front></TEI><istex><corpusName>wiley</corpusName><author><json:item><name>Nicolas Chartrel</name><affiliations><json:string>European Institute for Peptide Research, Laboratory of Cellular and Molecular Neuroendocrinology, INSERM U 413, UA CNRS, University of Rouen, 76821 Mont‐Saint‐Aignan, France</json:string></affiliations></json:item><json:item><name>J. Michael Conlon</name><affiliations><json:string>Regulatory Peptide Center, Department of Biomedical Sciences, Creighton University Medical School, Omaha, Nebraska 68178</json:string></affiliations></json:item><json:item><name>Françoise Collin</name><affiliations><json:string>European Institute for Peptide Research, Laboratory of Cellular and Molecular Neuroendocrinology, INSERM U 413, UA CNRS, University of Rouen, 76821 Mont‐Saint‐Aignan, France</json:string></affiliations></json:item><json:item><name>Benedicte Braun</name><affiliations><json:string>European Institute for Peptide Research, Laboratory of Cellular and Molecular Neuroendocrinology, INSERM U 413, UA CNRS, University of Rouen, 76821 Mont‐Saint‐Aignan, France</json:string></affiliations></json:item><json:item><name>David Waugh</name><affiliations><json:string>Regulatory Peptide Center, Department of Biomedical Sciences, Creighton University Medical School, Omaha, Nebraska 68178</json:string></affiliations></json:item><json:item><name>Mauro Vallarino</name><affiliations><json:string>Institute of Comparative Anatomy, University of Genova, 16132 Genova, Italy</json:string></affiliations></json:item><json:item><name>Sabine L. 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Rivier</name><affiliations><json:string>Clayton Foundation Laboratories for Peptide Biology, Salk Institute for Biological Studies, La Jolla, California 92037</json:string></affiliations></json:item><json:item><name>Hubert Vaudry</name><affiliations><json:string>European Institute for Peptide Research, Laboratory of Cellular and Molecular Neuroendocrinology, INSERM U 413, UA CNRS, University of Rouen, 76821 Mont‐Saint‐Aignan, France</json:string><json:string>European Institute for Peptide Research, Laboratory of Cellular and Molecular Neuroendocrinology, INSERM U 413, UA CNRS, University of Rouen, 76821 Mont‐Saint Aignan, France</json:string></affiliations></json:item></author><subject><json:item><lang><json:string>eng</json:string></lang><value>neuropeptides</value></json:item><json:item><lang><json:string>eng</json:string></lang><value>brain</value></json:item><json:item><lang><json:string>eng</json:string></lang><value>spinal cord</value></json:item><json:item><lang><json:string>eng</json:string></lang><value>motoneurons</value></json:item><json:item><lang><json:string>eng</json:string></lang><value>amphibians</value></json:item></subject><articleId><json:string>CNE10</json:string></articleId><language><json:string>eng</json:string></language><originalGenre><json:string>article</json:string></originalGenre><abstract>Urotensin II (UII) is traditionally regarded as a product of the neurosecretory cells in the caudal portion of the spinal cord of jawed fishes. A peptide related to UII has been recently isolated from the frog brain, thereby providing the first evidence that UII is also present in the central nervous system of a tetrapod. In the present study, we have investigated the distribution of UII‐immunoreactive elements in the brain and spinal cord of the frog Rana ridibunda by immunofluorescence using an antiserum directed against the conserved cyclic region of the peptide. Two distinct populations of UII‐immunoreactive perikarya were visualized. The first group of positive neurons was found in the nucleus hypoglossus of the medulla oblongata, which controls two striated muscles of the tongue. The second population of immunoreactive cell bodies was represented by a subset of motoneurons that were particularly abundant in the caudal region of the cord (34% of the motoneuron population). The telencephalon, diencephalon, mesencephalon, and metencephalon were totally devoid of UII‐containing cell bodies but displayed dense networks of UII‐immunoreactive fibers, notably in the thalamus, the tectum, the tegmentum, and the granular layer of the cerebellum. In addition, a dense bundle of long varicose processes projecting rostrocaudally was observed coursing along the ventral surface of the brain from the midtelencephalon to the medulla oblongata. Reversed‐phase high‐performance liquid chromatography analysis of frog brain, medulla oblongata, and spinal cord extracts revealed that, in all three regions, UII‐immunoreactive material eluted as a single peak which exhibited the same retention time as synthetic frog UII. Taken together, these data indicate that UII, in addition to its neuroendocrine functions in fish, is a potential regulatory peptide in the central nervous system of amphibians. © 1996 Wiley‐Liss, Inc.</abstract><qualityIndicators><score>8</score><pdfVersion>1.3</pdfVersion><pdfPageSize>612 x 792 pts (letter)</pdfPageSize><refBibsNative>true</refBibsNative><abstractCharCount>1931</abstractCharCount><pdfWordCount>6380</pdfWordCount><pdfCharCount>42319</pdfCharCount><pdfPageCount>16</pdfPageCount><abstractWordCount>285</abstractWordCount></qualityIndicators><title>Urotensin II in the central nervous system of the frog Rana ridibunda: immunohistochemical localization and biochemical characterization</title><genre><json:string>article</json:string></genre><host><volume>364</volume><publisherId><json:string>CNE</json:string></publisherId><pages><total>16</total><last>339</last><first>324</first></pages><issn><json:string>0021-9967</json:string></issn><issue>2</issue><genre><json:string>journal</json:string></genre><language><json:string>unknown</json:string></language><eissn><json:string>1096-9861</json:string></eissn><title>Journal of Comparative Neurology</title><doi><json:string>10.1002/(ISSN)1096-9861</json:string></doi></host><categories><wos><json:string>science</json:string><json:string>zoology</json:string><json:string>neurosciences</json:string></wos><scienceMetrix><json:string>health sciences</json:string><json:string>clinical medicine</json:string><json:string>neurology & neurosurgery</json:string></scienceMetrix></categories><publicationDate>1996</publicationDate><copyrightDate>1996</copyrightDate><doi><json:string>10.1002/(SICI)1096-9861(19960108)364:2>324::AID-CNE10>3.0.CO;2-P</json:string></doi><id>0FC60A28AEA01B2A55C1216FC4BD01C0453FB282</id><score>0.026727919</score><fulltext><json:item><extension>pdf</extension><original>true</original><mimetype>application/pdf</mimetype><uri>https://api.istex.fr/document/0FC60A28AEA01B2A55C1216FC4BD01C0453FB282/fulltext/pdf</uri></json:item><json:item><extension>zip</extension><original>false</original><mimetype>application/zip</mimetype><uri>https://api.istex.fr/document/0FC60A28AEA01B2A55C1216FC4BD01C0453FB282/fulltext/zip</uri></json:item><istex:fulltextTEI uri="https://api.istex.fr/document/0FC60A28AEA01B2A55C1216FC4BD01C0453FB282/fulltext/tei"><teiHeader><fileDesc><titleStmt><title level="a" type="main" xml:lang="en">Urotensin II in the central nervous system of the frog Rana ridibunda: immunohistochemical localization and biochemical characterization</title></titleStmt><publicationStmt><authority>ISTEX</authority><publisher>John Wiley & Sons, Inc.</publisher><pubPlace>New York</pubPlace><availability><p>Copyright © 1996 Wiley‐Liss, Inc.</p></availability><date>1996</date></publicationStmt><notesStmt><note>CNRS‐NSF and Galileo - No. 94022;</note><note>INSERM - No. 88‐6016;</note><note>NSF - No. INT‐921682;</note><note>CNR - No. 93, 00438, CT 04;</note><note>NIH - No. DK 26741;</note><note>Conseil Régional de Haute‐Normandic</note></notesStmt><sourceDesc><biblStruct type="inbook"><analytic><title level="a" type="main" xml:lang="en">Urotensin II in the central nervous system of the frog Rana ridibunda: immunohistochemical localization and biochemical characterization</title><author xml:id="author-1"><persName><forename type="first">Nicolas</forename><surname>Chartrel</surname></persName><affiliation>European Institute for Peptide Research, Laboratory of Cellular and Molecular Neuroendocrinology, INSERM U 413, UA CNRS, University of Rouen, 76821 Mont‐Saint‐Aignan, France</affiliation></author><author xml:id="author-2"><persName><forename type="first">J. Michael</forename><surname>Conlon</surname></persName><affiliation>Regulatory Peptide Center, Department of Biomedical Sciences, Creighton University Medical School, Omaha, Nebraska 68178</affiliation></author><author xml:id="author-3"><persName><forename type="first">Françoise</forename><surname>Collin</surname></persName><affiliation>European Institute for Peptide Research, Laboratory of Cellular and Molecular Neuroendocrinology, INSERM U 413, UA CNRS, University of Rouen, 76821 Mont‐Saint‐Aignan, France</affiliation></author><author xml:id="author-4"><persName><forename type="first">Benedicte</forename><surname>Braun</surname></persName><affiliation>European Institute for Peptide Research, Laboratory of Cellular and Molecular Neuroendocrinology, INSERM U 413, UA CNRS, University of Rouen, 76821 Mont‐Saint‐Aignan, France</affiliation></author><author xml:id="author-5"><persName><forename type="first">David</forename><surname>Waugh</surname></persName><affiliation>Regulatory Peptide Center, Department of Biomedical Sciences, Creighton University Medical School, Omaha, Nebraska 68178</affiliation></author><author xml:id="author-6"><persName><forename type="first">Mauro</forename><surname>Vallarino</surname></persName><affiliation>Institute of Comparative Anatomy, University of Genova, 16132 Genova, Italy</affiliation></author><author xml:id="author-7"><persName><forename type="first">Sabine L.</forename><surname>Lahrichi</surname></persName><affiliation>Clayton Foundation Laboratories for Peptide Biology, Salk Institute for Biological Studies, La Jolla, California 92037</affiliation></author><author xml:id="author-8"><persName><forename type="first">Jean E.</forename><surname>Rivier</surname></persName><affiliation>Clayton Foundation Laboratories for Peptide Biology, Salk Institute for Biological Studies, La Jolla, California 92037</affiliation></author><author xml:id="author-9"><persName><forename type="first">Hubert</forename><surname>Vaudry</surname></persName><affiliation>European Institute for Peptide Research, Laboratory of Cellular and Molecular Neuroendocrinology, INSERM U 413, UA CNRS, University of Rouen, 76821 Mont‐Saint‐Aignan, France</affiliation><affiliation>European Institute for Peptide Research, Laboratory of Cellular and Molecular Neuroendocrinology, INSERM U 413, UA CNRS, University of Rouen, 76821 Mont‐Saint Aignan, France</affiliation></author></analytic><monogr><title level="j">Journal of Comparative Neurology</title><title level="j" type="abbrev">J. Comp. Neurol.</title><idno type="pISSN">0021-9967</idno><idno type="eISSN">1096-9861</idno><idno type="DOI">10.1002/(ISSN)1096-9861</idno><imprint><publisher>John Wiley & Sons, Inc.</publisher><pubPlace>New York</pubPlace><date type="published" when="1996-01-08"></date><biblScope unit="volume">364</biblScope><biblScope unit="issue">2</biblScope><biblScope unit="page" from="324">324</biblScope><biblScope unit="page" to="339">339</biblScope></imprint></monogr><idno type="istex">0FC60A28AEA01B2A55C1216FC4BD01C0453FB282</idno><idno type="DOI">10.1002/(SICI)1096-9861(19960108)364:2<324::AID-CNE10>3.0.CO;2-P</idno><idno type="ArticleID">CNE10</idno></biblStruct></sourceDesc></fileDesc><profileDesc><creation><date>1996</date></creation><langUsage><language ident="en">en</language></langUsage><abstract xml:lang="en"><p>Urotensin II (UII) is traditionally regarded as a product of the neurosecretory cells in the caudal portion of the spinal cord of jawed fishes. A peptide related to UII has been recently isolated from the frog brain, thereby providing the first evidence that UII is also present in the central nervous system of a tetrapod. In the present study, we have investigated the distribution of UII‐immunoreactive elements in the brain and spinal cord of the frog Rana ridibunda by immunofluorescence using an antiserum directed against the conserved cyclic region of the peptide. Two distinct populations of UII‐immunoreactive perikarya were visualized. The first group of positive neurons was found in the nucleus hypoglossus of the medulla oblongata, which controls two striated muscles of the tongue. The second population of immunoreactive cell bodies was represented by a subset of motoneurons that were particularly abundant in the caudal region of the cord (34% of the motoneuron population). The telencephalon, diencephalon, mesencephalon, and metencephalon were totally devoid of UII‐containing cell bodies but displayed dense networks of UII‐immunoreactive fibers, notably in the thalamus, the tectum, the tegmentum, and the granular layer of the cerebellum. In addition, a dense bundle of long varicose processes projecting rostrocaudally was observed coursing along the ventral surface of the brain from the midtelencephalon to the medulla oblongata. Reversed‐phase high‐performance liquid chromatography analysis of frog brain, medulla oblongata, and spinal cord extracts revealed that, in all three regions, UII‐immunoreactive material eluted as a single peak which exhibited the same retention time as synthetic frog UII. Taken together, these data indicate that UII, in addition to its neuroendocrine functions in fish, is a potential regulatory peptide in the central nervous system of amphibians. © 1996 Wiley‐Liss, Inc.</p></abstract><textClass xml:lang="en"><keywords scheme="keyword"><list><head>keywords</head><item><term>neuropeptides</term></item><item><term>brain</term></item><item><term>spinal cord</term></item><item><term>motoneurons</term></item><item><term>amphibians</term></item></list></keywords></textClass></profileDesc><revisionDesc><change when="1995-07-11">Registration</change><change when="1996-01-08">Published</change></revisionDesc></teiHeader></istex:fulltextTEI><json:item><extension>txt</extension><original>false</original><mimetype>text/plain</mimetype><uri>https://api.istex.fr/document/0FC60A28AEA01B2A55C1216FC4BD01C0453FB282/fulltext/txt</uri></json:item></fulltext><metadata><istex:metadataXml wicri:clean="Wiley, elements deleted: body"><istex:xmlDeclaration>version="1.0" encoding="UTF-8" standalone="yes"</istex:xmlDeclaration><istex:document><component version="2.0" type="serialArticle" xml:lang="en"><header><publicationMeta level="product"><publisherInfo><publisherName>John Wiley & Sons, Inc.</publisherName><publisherLoc>New York</publisherLoc></publisherInfo><doi registered="yes">10.1002/(ISSN)1096-9861</doi><issn type="print">0021-9967</issn><issn type="electronic">1096-9861</issn><idGroup><id type="product" value="CNE"></id></idGroup><titleGroup><title type="main" xml:lang="en">Journal of Comparative Neurology</title><title type="short">J. Comp. Neurol.</title></titleGroup></publicationMeta><publicationMeta level="part" position="20"><doi origin="wiley" registered="yes">10.1002/(SICI)1096-9861(19960108)364:2<>1.0.CO;2-K</doi><numberingGroup><numbering type="journalVolume" number="364">364</numbering><numbering type="journalIssue">2</numbering></numberingGroup><coverDate startDate="1996-01-08">8 January 1996</coverDate></publicationMeta><publicationMeta level="unit" type="article" position="11" status="forIssue"><doi origin="wiley" registered="yes">10.1002/(SICI)1096-9861(19960108)364:2<324::AID-CNE10>3.0.CO;2-P</doi><idGroup><id type="unit" value="CNE10"></id></idGroup><countGroup><count type="pageTotal" number="16"></count></countGroup><copyright ownership="publisher">Copyright © 1996 Wiley‐Liss, Inc.</copyright><eventGroup><event type="manuscriptAccepted" date="1995-07-11"></event><event type="firstOnline" date="1998-12-06"></event><event type="publishedOnlineFinalForm" date="1998-12-06"></event><event type="xmlConverted" agent="Converter:JWSART34_TO_WML3G version:2.3.15 mode:FullText source:HeaderRef result:HeaderRef" date="2010-07-21"></event><event type="xmlConverted" agent="Converter:WILEY_ML3G_TO_WILEY_ML3GV2 version:3.8.8" date="2014-01-15"></event><event type="xmlConverted" agent="Converter:WML3G_To_WML3G version:4.1.7 mode:FullText,remove_FC" date="2014-10-24"></event></eventGroup><numberingGroup><numbering type="pageFirst">324</numbering><numbering type="pageLast">339</numbering></numberingGroup><correspondenceTo>European Institute for Peptide Research, Laboratory of Cellular and Molecular Neuroendocrinology, INSERM U 413, UA CNRS, University of Rouen, 76821 Mont‐Saint Aignan, France</correspondenceTo><linkGroup><link type="toTypesetVersion" href="file:CNE.CNE10.pdf"></link></linkGroup></publicationMeta><contentMeta><countGroup><count type="figureTotal" number="8"></count><count type="tableTotal" number="2"></count><count type="referenceTotal" number="52"></count></countGroup><titleGroup><title type="main" xml:lang="en">Urotensin II in the central nervous system of the frog <i>Rana ridibunda:</i> immunohistochemical localization and biochemical characterization</title></titleGroup><creators><creator xml:id="au1" creatorRole="author" affiliationRef="#af1"><personName><givenNames>Nicolas</givenNames><familyName>Chartrel</familyName></personName></creator><creator xml:id="au2" creatorRole="author" affiliationRef="#af2"><personName><givenNames>J. Michael</givenNames><familyName>Conlon</familyName></personName></creator><creator xml:id="au3" creatorRole="author" affiliationRef="#af1"><personName><givenNames>Françoise</givenNames><familyName>Collin</familyName></personName></creator><creator xml:id="au4" creatorRole="author" affiliationRef="#af1"><personName><givenNames>Benedicte</givenNames><familyName>Braun</familyName></personName></creator><creator xml:id="au5" creatorRole="author" affiliationRef="#af2"><personName><givenNames>David</givenNames><familyName>Waugh</familyName></personName></creator><creator xml:id="au6" creatorRole="author" affiliationRef="#af3"><personName><givenNames>Mauro</givenNames><familyName>Vallarino</familyName></personName></creator><creator xml:id="au7" creatorRole="author" affiliationRef="#af4"><personName><givenNames>Sabine L.</givenNames><familyName>Lahrichi</familyName></personName></creator><creator xml:id="au8" creatorRole="author" affiliationRef="#af4"><personName><givenNames>Jean E.</givenNames><familyName>Rivier</familyName></personName></creator><creator xml:id="au9" creatorRole="author" affiliationRef="#af1" corresponding="yes"><personName><givenNames>Hubert</givenNames><familyName>Vaudry</familyName></personName></creator></creators><affiliationGroup><affiliation xml:id="af1" countryCode="FR" type="organization"><unparsedAffiliation>European Institute for Peptide Research, Laboratory of Cellular and Molecular Neuroendocrinology, INSERM U 413, UA CNRS, University of Rouen, 76821 Mont‐Saint‐Aignan, France</unparsedAffiliation></affiliation><affiliation xml:id="af2" countryCode="US" type="organization"><unparsedAffiliation>Regulatory Peptide Center, Department of Biomedical Sciences, Creighton University Medical School, Omaha, Nebraska 68178</unparsedAffiliation></affiliation><affiliation xml:id="af3" countryCode="IT" type="organization"><unparsedAffiliation>Institute of Comparative Anatomy, University of Genova, 16132 Genova, Italy</unparsedAffiliation></affiliation><affiliation xml:id="af4" countryCode="US" type="organization"><unparsedAffiliation>Clayton Foundation Laboratories for Peptide Biology, Salk Institute for Biological Studies, La Jolla, California 92037</unparsedAffiliation></affiliation></affiliationGroup><keywordGroup xml:lang="en" type="author"><keyword xml:id="kwd1">neuropeptides</keyword><keyword xml:id="kwd2">brain</keyword><keyword xml:id="kwd3">spinal cord</keyword><keyword xml:id="kwd4">motoneurons</keyword><keyword xml:id="kwd5">amphibians</keyword></keywordGroup><fundingInfo><fundingAgency>CNRS‐NSF and Galileo</fundingAgency><fundingNumber>94022</fundingNumber></fundingInfo><fundingInfo><fundingAgency>INSERM</fundingAgency><fundingNumber>88‐6016</fundingNumber></fundingInfo><fundingInfo><fundingAgency>NSF</fundingAgency><fundingNumber>INT‐921682</fundingNumber></fundingInfo><fundingInfo><fundingAgency>CNR</fundingAgency><fundingNumber>93, 00438, CT 04</fundingNumber></fundingInfo><fundingInfo><fundingAgency>NIH</fundingAgency><fundingNumber>DK 26741</fundingNumber></fundingInfo><fundingInfo><fundingAgency>Conseil Régional de Haute‐Normandic</fundingAgency></fundingInfo><abstractGroup><abstract type="main" xml:lang="en"><title type="main">Abstract</title><p>Urotensin II (UII) is traditionally regarded as a product of the neurosecretory cells in the caudal portion of the spinal cord of jawed fishes. A peptide related to UII has been recently isolated from the frog brain, thereby providing the first evidence that UII is also present in the central nervous system of a tetrapod. In the present study, we have investigated the distribution of UII‐immunoreactive elements in the brain and spinal cord of the frog <i>Rana ridibunda</i> by immunofluorescence using an antiserum directed against the conserved cyclic region of the peptide. Two distinct populations of UII‐immunoreactive perikarya were visualized. The first group of positive neurons was found in the nucleus hypoglossus of the medulla oblongata, which controls two striated muscles of the tongue. The second population of immunoreactive cell bodies was represented by a subset of motoneurons that were particularly abundant in the caudal region of the cord (34% of the motoneuron population). The telencephalon, diencephalon, mesencephalon, and metencephalon were totally devoid of UII‐containing cell bodies but displayed dense networks of UII‐immunoreactive fibers, notably in the thalamus, the tectum, the tegmentum, and the granular layer of the cerebellum. In addition, a dense bundle of long varicose processes projecting rostrocaudally was observed coursing along the ventral surface of the brain from the midtelencephalon to the medulla oblongata. Reversed‐phase high‐performance liquid chromatography analysis of frog brain, medulla oblongata, and spinal cord extracts revealed that, in all three regions, UII‐immunoreactive material eluted as a single peak which exhibited the same retention time as synthetic frog UII. Taken together, these data indicate that UII, in addition to its neuroendocrine functions in fish, is a potential regulatory peptide in the central nervous system of amphibians. © 1996 Wiley‐Liss, Inc.</p></abstract></abstractGroup></contentMeta></header></component></istex:document></istex:metadataXml><mods version="3.6"><titleInfo lang="en"><title>Urotensin II in the central nervous system of the frog Rana ridibunda: immunohistochemical localization and biochemical characterization</title></titleInfo><titleInfo type="alternative" contentType="CDATA" lang="en"><title>Urotensin II in the central nervous system of the frog Rana ridibunda: immunohistochemical localization and biochemical characterization</title></titleInfo><name type="personal"><namePart type="given">Nicolas</namePart><namePart type="family">Chartrel</namePart><affiliation>European Institute for Peptide Research, Laboratory of Cellular and Molecular Neuroendocrinology, INSERM U 413, UA CNRS, University of Rouen, 76821 Mont‐Saint‐Aignan, France</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">J. Michael</namePart><namePart type="family">Conlon</namePart><affiliation>Regulatory Peptide Center, Department of Biomedical Sciences, Creighton University Medical School, Omaha, Nebraska 68178</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">Françoise</namePart><namePart type="family">Collin</namePart><affiliation>European Institute for Peptide Research, Laboratory of Cellular and Molecular Neuroendocrinology, INSERM U 413, UA CNRS, University of Rouen, 76821 Mont‐Saint‐Aignan, France</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">Benedicte</namePart><namePart type="family">Braun</namePart><affiliation>European Institute for Peptide Research, Laboratory of Cellular and Molecular Neuroendocrinology, INSERM U 413, UA CNRS, University of Rouen, 76821 Mont‐Saint‐Aignan, France</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">David</namePart><namePart type="family">Waugh</namePart><affiliation>Regulatory Peptide Center, Department of Biomedical Sciences, Creighton University Medical School, Omaha, Nebraska 68178</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">Mauro</namePart><namePart type="family">Vallarino</namePart><affiliation>Institute of Comparative Anatomy, University of Genova, 16132 Genova, Italy</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">Sabine L.</namePart><namePart type="family">Lahrichi</namePart><affiliation>Clayton Foundation Laboratories for Peptide Biology, Salk Institute for Biological Studies, La Jolla, California 92037</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">Jean E.</namePart><namePart type="family">Rivier</namePart><affiliation>Clayton Foundation Laboratories for Peptide Biology, Salk Institute for Biological Studies, La Jolla, California 92037</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">Hubert</namePart><namePart type="family">Vaudry</namePart><affiliation>European Institute for Peptide Research, Laboratory of Cellular and Molecular Neuroendocrinology, INSERM U 413, UA CNRS, University of Rouen, 76821 Mont‐Saint‐Aignan, France</affiliation><affiliation>European Institute for Peptide Research, Laboratory of Cellular and Molecular Neuroendocrinology, INSERM U 413, UA CNRS, University of Rouen, 76821 Mont‐Saint Aignan, France</affiliation><role><roleTerm type="text">author</roleTerm></role></name><typeOfResource>text</typeOfResource><genre type="article" displayLabel="article"></genre><originInfo><publisher>John Wiley & Sons, Inc.</publisher><place><placeTerm type="text">New York</placeTerm></place><dateIssued encoding="w3cdtf">1996-01-08</dateIssued><dateValid encoding="w3cdtf">1995-07-11</dateValid><copyrightDate encoding="w3cdtf">1996</copyrightDate></originInfo><language><languageTerm type="code" authority="rfc3066">en</languageTerm><languageTerm type="code" authority="iso639-2b">eng</languageTerm></language><physicalDescription><internetMediaType>text/html</internetMediaType><extent unit="figures">8</extent><extent unit="tables">2</extent><extent unit="references">52</extent></physicalDescription><abstract lang="en">Urotensin II (UII) is traditionally regarded as a product of the neurosecretory cells in the caudal portion of the spinal cord of jawed fishes. A peptide related to UII has been recently isolated from the frog brain, thereby providing the first evidence that UII is also present in the central nervous system of a tetrapod. In the present study, we have investigated the distribution of UII‐immunoreactive elements in the brain and spinal cord of the frog Rana ridibunda by immunofluorescence using an antiserum directed against the conserved cyclic region of the peptide. Two distinct populations of UII‐immunoreactive perikarya were visualized. The first group of positive neurons was found in the nucleus hypoglossus of the medulla oblongata, which controls two striated muscles of the tongue. The second population of immunoreactive cell bodies was represented by a subset of motoneurons that were particularly abundant in the caudal region of the cord (34% of the motoneuron population). The telencephalon, diencephalon, mesencephalon, and metencephalon were totally devoid of UII‐containing cell bodies but displayed dense networks of UII‐immunoreactive fibers, notably in the thalamus, the tectum, the tegmentum, and the granular layer of the cerebellum. In addition, a dense bundle of long varicose processes projecting rostrocaudally was observed coursing along the ventral surface of the brain from the midtelencephalon to the medulla oblongata. Reversed‐phase high‐performance liquid chromatography analysis of frog brain, medulla oblongata, and spinal cord extracts revealed that, in all three regions, UII‐immunoreactive material eluted as a single peak which exhibited the same retention time as synthetic frog UII. Taken together, these data indicate that UII, in addition to its neuroendocrine functions in fish, is a potential regulatory peptide in the central nervous system of amphibians. © 1996 Wiley‐Liss, Inc.</abstract><note type="funding">CNRS‐NSF and Galileo - No. 94022; </note><note type="funding">INSERM - No. 88‐6016; </note><note type="funding">NSF - No. INT‐921682; </note><note type="funding">CNR - No. 93, 00438, CT 04; </note><note type="funding">NIH - No. DK 26741; </note><note type="funding">Conseil Régional de Haute‐Normandic</note><subject lang="en"><genre>keywords</genre><topic>neuropeptides</topic><topic>brain</topic><topic>spinal cord</topic><topic>motoneurons</topic><topic>amphibians</topic></subject><relatedItem type="host"><titleInfo><title>Journal of Comparative Neurology</title></titleInfo><titleInfo type="abbreviated"><title>J. Comp. Neurol.</title></titleInfo><genre type="journal">journal</genre><identifier type="ISSN">0021-9967</identifier><identifier type="eISSN">1096-9861</identifier><identifier type="DOI">10.1002/(ISSN)1096-9861</identifier><identifier type="PublisherID">CNE</identifier><part><date>1996</date><detail type="volume"><caption>vol.</caption><number>364</number></detail><detail type="issue"><caption>no.</caption><number>2</number></detail><extent unit="pages"><start>324</start><end>339</end><total>16</total></extent></part></relatedItem><identifier type="istex">0FC60A28AEA01B2A55C1216FC4BD01C0453FB282</identifier><identifier type="DOI">10.1002/(SICI)1096-9861(19960108)364:2<324::AID-CNE10>3.0.CO;2-P</identifier><identifier type="ArticleID">CNE10</identifier><accessCondition type="use and reproduction" contentType="copyright">Copyright © 1996 Wiley‐Liss, Inc.</accessCondition><recordInfo><recordContentSource>WILEY</recordContentSource><recordOrigin>John Wiley & Sons, Inc.</recordOrigin></recordInfo></mods></metadata><serie></serie></istex></record>Pour manipuler ce document sous Unix (Dilib)
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