Ultrastructure and osmoregulatory function of the kidney in larvae of the Persian sturgeon Acipenser persicus
Identifieur interne : 001350 ( Istex/Corpus ); précédent : 001349; suivant : 001351Ultrastructure and osmoregulatory function of the kidney in larvae of the Persian sturgeon Acipenser persicus
Auteurs : Z. Taghizadeh Rahmat Abadi ; S. Khodabandeh ; B. Abtahi ; G. Charmantier ; M. Charmantier-DauresSource :
- Journal of Fish Biology [ 0022-1112 ] ; 2011-05.
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- KwdEn :
Abstract
The localization of Na+, K+‐ATPase (NKA) and the ultrastructural features of kidney were examined in larvae of the Persian sturgeon Acipenser persicus (L 31–41 mm total length and 182·3–417·3 mg). Investigations were conducted through light and electron microscopy and through immunofluorescence for NKA detection. The kidney nephrons consisted of a large glomerulus and tubules (neck, proximal, distal and collecting), which connected to the ureters. Posteriorly, ureters extended and joined together into a thin‐walled ureter terminal sac. Ultrastructurally, the glomerular cells (podocytes) possessed distinctive pedicels that extended to the basal membrane. The proximal tubule (PT) showed two different cells. The cells lining the anterior part of PT possessed apical tall microvilli (c. 2·7 µm), a sub‐apical tubular system, a basal nucleus and dense granules. Posteriorly in the cells, the sub‐apical tubular system and granules were absent and round mitochondria associated with basolateral infoldings were found; the apical microvilli were reduced. Distal and collecting tubular cells showed the typical features of osmoregulatory cells, i.e. well‐developed basolateral infoldings associated with numerous mitochondria. No immunofluorescence of NKA was detected in the glomeruli. A weak immunostaining was observed at the basolateral side of the cells lining the neck and PT. A strong immunostaining of NKA was observed in the entire cells of the distal tubules, collecting tubules and in some isolated cells of the ureters. In all immunostained cells, the basolateral region showed a much higher fluorescence and nuclei were immunonegative. In conclusion, the epithelial cells of kidney tubules had morphological and enzymatic features of ionocytes, particularly in the distal and collecting tubules. Thus, the kidney of A. persicus larvae possesses active ion exchange capabilities and, beside its implication in excretion, participates in osmoregulation.
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DOI: 10.1111/j.1095-8649.2011.02939.x
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Investigations were conducted through light and electron microscopy and through immunofluorescence for NKA detection. The kidney nephrons consisted of a large glomerulus and tubules (neck, proximal, distal and collecting), which connected to the ureters. Posteriorly, ureters extended and joined together into a thin‐walled ureter terminal sac. Ultrastructurally, the glomerular cells (podocytes) possessed distinctive pedicels that extended to the basal membrane. The proximal tubule (PT) showed two different cells. The cells lining the anterior part of PT possessed apical tall microvilli (c. 2·7 µm), a sub‐apical tubular system, a basal nucleus and dense granules. Posteriorly in the cells, the sub‐apical tubular system and granules were absent and round mitochondria associated with basolateral infoldings were found; the apical microvilli were reduced. Distal and collecting tubular cells showed the typical features of osmoregulatory cells, i.e. well‐developed basolateral infoldings associated with numerous mitochondria. No immunofluorescence of NKA was detected in the glomeruli. A weak immunostaining was observed at the basolateral side of the cells lining the neck and PT. A strong immunostaining of NKA was observed in the entire cells of the distal tubules, collecting tubules and in some isolated cells of the ureters. In all immunostained cells, the basolateral region showed a much higher fluorescence and nuclei were immunonegative. In conclusion, the epithelial cells of kidney tubules had morphological and enzymatic features of ionocytes, particularly in the distal and collecting tubules. Thus, the kidney of A. persicus larvae possesses active ion exchange capabilities and, beside its implication in excretion, participates in osmoregulation.</div></front></TEI><istex><corpusName>wiley</corpusName><author><json:item><name>Z. 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Distal and collecting tubular cells showed the typical features of osmoregulatory cells, i.e. well‐developed basolateral infoldings associated with numerous mitochondria. No immunofluorescence of NKA was detected in the glomeruli. A weak immunostaining was observed at the basolateral side of the cells lining the neck and PT. A strong immunostaining of NKA was observed in the entire cells of the distal tubules, collecting tubules and in some isolated cells of the ureters. In all immunostained cells, the basolateral region showed a much higher fluorescence and nuclei were immunonegative. In conclusion, the epithelial cells of kidney tubules had morphological and enzymatic features of ionocytes, particularly in the distal and collecting tubules. 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Investigations were conducted through light and electron microscopy and through immunofluorescence for NKA detection. The kidney nephrons consisted of a large glomerulus and tubules (neck, proximal, distal and collecting), which connected to the ureters. Posteriorly, ureters extended and joined together into a thin‐walled ureter terminal sac. Ultrastructurally, the glomerular cells (podocytes) possessed distinctive pedicels that extended to the basal membrane. The proximal tubule (PT) showed two different cells. The cells lining the anterior part of PT possessed apical tall microvilli (c. 2·7 µm), a sub‐apical tubular system, a basal nucleus and dense granules. Posteriorly in the cells, the sub‐apical tubular system and granules were absent and round mitochondria associated with basolateral infoldings were found; the apical microvilli were reduced. Distal and collecting tubular cells showed the typical features of osmoregulatory cells, i.e. well‐developed basolateral infoldings associated with numerous mitochondria. No immunofluorescence of NKA was detected in the glomeruli. A weak immunostaining was observed at the basolateral side of the cells lining the neck and PT. A strong immunostaining of NKA was observed in the entire cells of the distal tubules, collecting tubules and in some isolated cells of the ureters. In all immunostained cells, the basolateral region showed a much higher fluorescence and nuclei were immunonegative. In conclusion, the epithelial cells of kidney tubules had morphological and enzymatic features of ionocytes, particularly in the distal and collecting tubules. 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Journal of Fish Biology © 2011 The Fisheries Society of the British Isles</copyright><eventGroup><event type="xmlConverted" agent="Converter:BPG_TO_WML3G version:2.4.8 mode:FullText" date="2011-05-03"></event><event type="publishedOnlineEarlyUnpaginated" date="2011-04-11"></event><event type="firstOnline" date="2011-04-11"></event><event type="publishedOnlineFinalForm" date="2011-05-03"></event><event type="xmlConverted" agent="Converter:WILEY_ML3G_TO_WILEY_ML3GV2 version:3.8.8" date="2014-01-30"></event><event type="xmlConverted" agent="Converter:WML3G_To_WML3G version:4.1.7 mode:FullText,remove_FC" date="2014-10-30"></event></eventGroup><numberingGroup><numbering type="pageFirst" number="1359">1359</numbering><numbering type="pageLast" number="1374">1374</numbering></numberingGroup><correspondenceTo> Tel.: +98 9144472033; email: <email>surp78@gmail.com</email></correspondenceTo><linkGroup><link type="toTypesetVersion" href="file:JFB.JFB2939.pdf"></link></linkGroup></publicationMeta><contentMeta><unparsedEditorialHistory>(Received 14 November 2009, Accepted 5 February 2011)</unparsedEditorialHistory><countGroup><count type="figureTotal" number="5"></count><count type="tableTotal" number="0"></count><count type="formulaTotal" number="0"></count><count type="referenceTotal" number="48"></count></countGroup><titleGroup><title type="main">Ultrastructure and osmoregulatory function of the kidney in larvae of the Persian sturgeon <i>Acipenser persicus</i></title><title type="shortAuthors">Z. TAGHIZADEH RAHMAT ABADI <i>ET AL</i>.</title></titleGroup><creators><creator creatorRole="author" xml:id="cr1" affiliationRef="#a1"><personName><givenNames>Z.</givenNames><familyName>Taghizadeh Rahmat Abadi</familyName></personName></creator><creator creatorRole="author" xml:id="cr2" affiliationRef="#a1" corresponding="yes"><personName><givenNames>S.</givenNames><familyName>Khodabandeh</familyName></personName></creator><creator creatorRole="author" xml:id="cr3" affiliationRef="#a2"><personName><givenNames>B.</givenNames><familyName>Abtahi</familyName></personName></creator><creator creatorRole="author" xml:id="cr4" affiliationRef="#a3"><personName><givenNames>G.</givenNames><familyName>Charmantier</familyName></personName></creator><creator creatorRole="author" xml:id="cr5" affiliationRef="#a3"><personName><givenNames>M.</givenNames><familyName>Charmantier‐Daures</familyName></personName></creator></creators><affiliationGroup><affiliation xml:id="a1"><unparsedAffiliation><i>Marine Biology Group, Faculty of Marine Sciences, Tarbait Modares University, Tehran, Iran</i></unparsedAffiliation></affiliation><affiliation xml:id="a2"><unparsedAffiliation><i>Faculty of Biology, Shahid Beheshti University, G.C., Tehran, Iran</i></unparsedAffiliation></affiliation><affiliation xml:id="a3" countryCode="FR"><unparsedAffiliation><i>Equipe AEO, UMR 5119 Ecolag, Université Montpellier 2, 34095 Montpellier, France</i></unparsedAffiliation></affiliation></affiliationGroup><keywordGroup xml:lang="en"><keyword xml:id="k1">immunocytochemistry</keyword><keyword xml:id="k2">ionocytes</keyword><keyword xml:id="k3">mesonephros</keyword><keyword xml:id="k4">Na<sup>+</sup></keyword><keyword xml:id="k5">K<sup>+</sup>‐ATPase</keyword><keyword xml:id="k6">osmoregulation</keyword></keywordGroup><abstractGroup><abstract type="main" xml:lang="en"><p>The localization of Na<sup>+</sup>, K<sup>+</sup>‐ATPase (NKA) and the ultrastructural features of kidney were examined in larvae of the Persian sturgeon <i>Acipenser persicus</i> (L 31–41 mm total length and 182·3–417·3 mg). Investigations were conducted through light and electron microscopy and through immunofluorescence for NKA detection. The kidney nephrons consisted of a large glomerulus and tubules (neck, proximal, distal and collecting), which connected to the ureters. Posteriorly, ureters extended and joined together into a thin‐walled ureter terminal sac. Ultrastructurally, the glomerular cells (podocytes) possessed distinctive pedicels that extended to the basal membrane. The proximal tubule (PT) showed two different cells. The cells lining the anterior part of PT possessed apical tall microvilli (<i>c.</i> 2·7 µm), a sub‐apical tubular system, a basal nucleus and dense granules. Posteriorly in the cells, the sub‐apical tubular system and granules were absent and round mitochondria associated with basolateral infoldings were found; the apical microvilli were reduced. Distal and collecting tubular cells showed the typical features of osmoregulatory cells, <i>i.e.</i> well‐developed basolateral infoldings associated with numerous mitochondria. No immunofluorescence of NKA was detected in the glomeruli. A weak immunostaining was observed at the basolateral side of the cells lining the neck and PT. A strong immunostaining of NKA was observed in the entire cells of the distal tubules, collecting tubules and in some isolated cells of the ureters. In all immunostained cells, the basolateral region showed a much higher fluorescence and nuclei were immunonegative. In conclusion, the epithelial cells of kidney tubules had morphological and enzymatic features of ionocytes, particularly in the distal and collecting tubules. Thus, the kidney of <i>A. persicus</i> larvae possesses active ion exchange capabilities and, beside its implication in excretion, participates in osmoregulation.</p></abstract></abstractGroup></contentMeta></header></component></istex:document></istex:metadataXml><mods version="3.6"><titleInfo lang="en"><title>Ultrastructure and osmoregulatory function of the kidney in larvae of the Persian sturgeon Acipenser persicus</title></titleInfo><titleInfo type="alternative" contentType="CDATA" lang="en"><title>Ultrastructure and osmoregulatory function of the kidney in larvae of the Persian sturgeon Acipenser persicus</title></titleInfo><name type="personal"><namePart type="given">Z.</namePart><namePart type="family">Taghizadeh Rahmat Abadi</namePart><affiliation>Marine Biology Group, Faculty of Marine Sciences, Tarbait Modares University, Tehran, Iran</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">S.</namePart><namePart type="family">Khodabandeh</namePart><affiliation>Marine Biology Group, Faculty of Marine Sciences, Tarbait Modares University, Tehran, Iran</affiliation><affiliation>E-mail: surp78@gmail.com</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">B.</namePart><namePart type="family">Abtahi</namePart><affiliation>Faculty of Biology, Shahid Beheshti University, G.C., Tehran, Iran</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">G.</namePart><namePart type="family">Charmantier</namePart><affiliation>Equipe AEO, UMR 5119 Ecolag, Université Montpellier 2, 34095 Montpellier, France</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">M.</namePart><namePart type="family">Charmantier‐Daures</namePart><affiliation>Equipe AEO, UMR 5119 Ecolag, Université Montpellier 2, 34095 Montpellier, France</affiliation><role><roleTerm type="text">author</roleTerm></role></name><typeOfResource>text</typeOfResource><genre type="article" displayLabel="article"></genre><originInfo><publisher>Blackwell Publishing Ltd</publisher><place><placeTerm type="text">Oxford, UK</placeTerm></place><dateIssued encoding="w3cdtf">2011-05</dateIssued><edition>(Received 14 November 2009, Accepted 5 February 2011)</edition><copyrightDate encoding="w3cdtf">2011</copyrightDate></originInfo><language><languageTerm type="code" authority="rfc3066">en</languageTerm><languageTerm type="code" authority="iso639-2b">eng</languageTerm></language><physicalDescription><internetMediaType>text/html</internetMediaType><extent unit="figures">5</extent><extent unit="references">48</extent></physicalDescription><abstract lang="en">The localization of Na+, K+‐ATPase (NKA) and the ultrastructural features of kidney were examined in larvae of the Persian sturgeon Acipenser persicus (L 31–41 mm total length and 182·3–417·3 mg). Investigations were conducted through light and electron microscopy and through immunofluorescence for NKA detection. The kidney nephrons consisted of a large glomerulus and tubules (neck, proximal, distal and collecting), which connected to the ureters. Posteriorly, ureters extended and joined together into a thin‐walled ureter terminal sac. Ultrastructurally, the glomerular cells (podocytes) possessed distinctive pedicels that extended to the basal membrane. The proximal tubule (PT) showed two different cells. The cells lining the anterior part of PT possessed apical tall microvilli (c. 2·7 µm), a sub‐apical tubular system, a basal nucleus and dense granules. Posteriorly in the cells, the sub‐apical tubular system and granules were absent and round mitochondria associated with basolateral infoldings were found; the apical microvilli were reduced. Distal and collecting tubular cells showed the typical features of osmoregulatory cells, i.e. well‐developed basolateral infoldings associated with numerous mitochondria. No immunofluorescence of NKA was detected in the glomeruli. A weak immunostaining was observed at the basolateral side of the cells lining the neck and PT. A strong immunostaining of NKA was observed in the entire cells of the distal tubules, collecting tubules and in some isolated cells of the ureters. In all immunostained cells, the basolateral region showed a much higher fluorescence and nuclei were immunonegative. In conclusion, the epithelial cells of kidney tubules had morphological and enzymatic features of ionocytes, particularly in the distal and collecting tubules. Thus, the kidney of A. persicus larvae possesses active ion exchange capabilities and, beside its implication in excretion, participates in osmoregulation.</abstract><subject lang="en"><genre>keywords</genre><topic>immunocytochemistry</topic><topic>ionocytes</topic><topic>mesonephros</topic><topic>Na+</topic><topic>K+‐ATPase</topic><topic>osmoregulation</topic></subject><relatedItem type="host"><titleInfo><title>Journal of Fish Biology</title></titleInfo><genre type="journal">journal</genre><identifier type="ISSN">0022-1112</identifier><identifier type="eISSN">1095-8649</identifier><identifier type="DOI">10.1111/(ISSN)1095-8649</identifier><identifier type="PublisherID">JFB</identifier><part><date>2011</date><detail type="volume"><caption>vol.</caption><number>78</number></detail><detail type="issue"><caption>no.</caption><number>5</number></detail><extent unit="pages"><start>1359</start><end>1374</end><total>16</total></extent></part></relatedItem><identifier type="istex">2D3E8098A95E18E240B57385CDA24E4DD7B3FF5A</identifier><identifier type="DOI">10.1111/j.1095-8649.2011.02939.x</identifier><identifier type="ArticleID">JFB2939</identifier><accessCondition type="use and reproduction" contentType="copyright">© 2011 The Authors. Journal of Fish Biology © 2011 The Fisheries Society of the British Isles</accessCondition><recordInfo><recordContentSource>WILEY</recordContentSource><recordOrigin>Blackwell Publishing Ltd</recordOrigin></recordInfo></mods></metadata><serie></serie></istex></record>Pour manipuler ce document sous Unix (Dilib)
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