Measurement of vitellogenin, a biomarker for exposure to oestrogenic chemicals, in a wide variety of cyprinid fish
Identifieur interne : 001292 ( Istex/Corpus ); précédent : 001291; suivant : 001293Measurement of vitellogenin, a biomarker for exposure to oestrogenic chemicals, in a wide variety of cyprinid fish
Auteurs : C. R. Tyler ; B. Van Der Eerden ; S. Jobling ; G. Panter ; J. P. SumpterSource :
- Journal of Comparative Physiology B [ 0174-1578 ] ; 1996-11-01.
Abstract
Abstract: There is increasing concern about man-made chemicals in the aquatic environment that mimic oestrogens because they may disrupt reproductive function. Vitellogenin, a precursor of egg-yolk in fish and other oviparous animals, may be used as a biomarker for “oestrogen” exposure. This study investigated the use of a radioimmunoassay developed to carp (Cyprinus carpio) vitellogenin to measure vitellogenin in other species of fish, especially cyprinids that would be of value for field and laboratory studies on oestrogenic xenobiotics. Of the nine families of fish studied, only vitellogenin from cyprinids (to which the carp belongs) showed good cross-reactivity in the carp vitellogenin radioimmunoassay. Vitellogenin from cyprinids native to Europe that cross reacted in the carp vitellogenin radioimmunoassay included: bream (Abramis brama), roach (Rutilus rutilus), rudd (Scardinius erythropthalmus), gudgeon (Gobio gobio) and minnow (Phoxinus phoxinus). Vitellogenin from cyprinids used widely in ecotoxicology that cross reacted in the carp vitellogenin radioimmunoassay included: fathead minnow (Pimephales promelas), zebrafish (Brachydanio rerio) and goldfish (Carassius auratus). In the cyprinids studies, the concentrations of vitellogenin in mature females were between a few hundred and a thousand microgram per millilitre. Concentrations of plasma vitellogenin in immature females were always greater than 200 ng·m-1, whereas in males (with the exception of the fathead minnow) plasma vitellogenin concentrations were less than 20 ng·ml-1 (and generally, much lower). The results suggest that the structure of vitellogenin is highly conserved within the cyprinid family and that the carp vitellogenin radioimmunoassay may be used to measure the concentrations of vitellogenin in plasma from a wide variety of cyprinids.
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DOI: 10.1007/BF02337886
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Vitellogenin, a precursor of egg-yolk in fish and other oviparous animals, may be used as a biomarker for “oestrogen” exposure. This study investigated the use of a radioimmunoassay developed to carp (Cyprinus carpio) vitellogenin to measure vitellogenin in other species of fish, especially cyprinids that would be of value for field and laboratory studies on oestrogenic xenobiotics. Of the nine families of fish studied, only vitellogenin from cyprinids (to which the carp belongs) showed good cross-reactivity in the carp vitellogenin radioimmunoassay. Vitellogenin from cyprinids native to Europe that cross reacted in the carp vitellogenin radioimmunoassay included: bream (Abramis brama), roach (Rutilus rutilus), rudd (Scardinius erythropthalmus), gudgeon (Gobio gobio) and minnow (Phoxinus phoxinus). Vitellogenin from cyprinids used widely in ecotoxicology that cross reacted in the carp vitellogenin radioimmunoassay included: fathead minnow (Pimephales promelas), zebrafish (Brachydanio rerio) and goldfish (Carassius auratus). In the cyprinids studies, the concentrations of vitellogenin in mature females were between a few hundred and a thousand microgram per millilitre. Concentrations of plasma vitellogenin in immature females were always greater than 200 ng·m-1, whereas in males (with the exception of the fathead minnow) plasma vitellogenin concentrations were less than 20 ng·ml-1 (and generally, much lower). The results suggest that the structure of vitellogenin is highly conserved within the cyprinid family and that the carp vitellogenin radioimmunoassay may be used to measure the concentrations of vitellogenin in plasma from a wide variety of cyprinids.</div></front></TEI><istex><corpusName>springer</corpusName><author><json:item><name>C. R. 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Vitellogenin, a precursor of egg-yolk in fish and other oviparous animals, may be used as a biomarker for “oestrogen” exposure. This study investigated the use of a radioimmunoassay developed to carp (Cyprinus carpio) vitellogenin to measure vitellogenin in other species of fish, especially cyprinids that would be of value for field and laboratory studies on oestrogenic xenobiotics. Of the nine families of fish studied, only vitellogenin from cyprinids (to which the carp belongs) showed good cross-reactivity in the carp vitellogenin radioimmunoassay. Vitellogenin from cyprinids native to Europe that cross reacted in the carp vitellogenin radioimmunoassay included: bream (Abramis brama), roach (Rutilus rutilus), rudd (Scardinius erythropthalmus), gudgeon (Gobio gobio) and minnow (Phoxinus phoxinus). Vitellogenin from cyprinids used widely in ecotoxicology that cross reacted in the carp vitellogenin radioimmunoassay included: fathead minnow (Pimephales promelas), zebrafish (Brachydanio rerio) and goldfish (Carassius auratus). In the cyprinids studies, the concentrations of vitellogenin in mature females were between a few hundred and a thousand microgram per millilitre. Concentrations of plasma vitellogenin in immature females were always greater than 200 ng·m-1, whereas in males (with the exception of the fathead minnow) plasma vitellogenin concentrations were less than 20 ng·ml-1 (and generally, much lower). The results suggest that the structure of vitellogenin is highly conserved within the cyprinid family and that the carp vitellogenin radioimmunoassay may be used to measure the concentrations of vitellogenin in plasma from a wide variety of cyprinids.</abstract><qualityIndicators><score>8</score><pdfVersion>1.1</pdfVersion><pdfPageSize>580 x 832 pts</pdfPageSize><refBibsNative>false</refBibsNative><keywordCount>8</keywordCount><abstractCharCount>1843</abstractCharCount><pdfWordCount>5244</pdfWordCount><pdfCharCount>32155</pdfCharCount><pdfPageCount>9</pdfPageCount><abstractWordCount>255</abstractWordCount></qualityIndicators><title>Measurement of vitellogenin, a biomarker for exposure to oestrogenic chemicals, in a wide variety of cyprinid fish</title><genre><json:string>research-article</json:string></genre><host><volume>166</volume><pages><last>426</last><first>418</first></pages><issn><json:string>0174-1578</json:string></issn><issue>7</issue><subject><json:item><value>Biomedicine 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Vitellogenin, a precursor of egg-yolk in fish and other oviparous animals, may be used as a biomarker for “oestrogen” exposure. This study investigated the use of a radioimmunoassay developed to carp (Cyprinus carpio) vitellogenin to measure vitellogenin in other species of fish, especially cyprinids that would be of value for field and laboratory studies on oestrogenic xenobiotics. Of the nine families of fish studied, only vitellogenin from cyprinids (to which the carp belongs) showed good cross-reactivity in the carp vitellogenin radioimmunoassay. Vitellogenin from cyprinids native to Europe that cross reacted in the carp vitellogenin radioimmunoassay included: bream (Abramis brama), roach (Rutilus rutilus), rudd (Scardinius erythropthalmus), gudgeon (Gobio gobio) and minnow (Phoxinus phoxinus). Vitellogenin from cyprinids used widely in ecotoxicology that cross reacted in the carp vitellogenin radioimmunoassay included: fathead minnow (Pimephales promelas), zebrafish (Brachydanio rerio) and goldfish (Carassius auratus). In the cyprinids studies, the concentrations of vitellogenin in mature females were between a few hundred and a thousand microgram per millilitre. Concentrations of plasma vitellogenin in immature females were always greater than 200 ng·m-1, whereas in males (with the exception of the fathead minnow) plasma vitellogenin concentrations were less than 20 ng·ml-1 (and generally, much lower). The results suggest that the structure of vitellogenin is highly conserved within the cyprinid family and that the carp vitellogenin radioimmunoassay may be used to measure the concentrations of vitellogenin in plasma from a wide variety of cyprinids.</p></abstract><textClass><keywords scheme="keyword"><list><head>Abbreviations</head><item><term>PCBs</term><term>polychlorinated biphenyls</term></item><item><term>PAHs</term><term>polycyclic aromatic hydrocarbons</term></item><item><term>c-VTG</term><term>carp vitellogenin</term></item><item><term>GSI</term><term>gonadosomatic index</term></item><item><term>ELISA</term><term>enzyme-linked immunosorbant assay</term></item><item><term>VTG</term><term>vitellogenin</term></item><item><term>RJA</term><term>radioimmunoassay</term></item><item><term>STWs</term><term>sewage treatment works</term></item></list></keywords></textClass><textClass><keywords scheme="Journal Subject"><list><head>Life Sciences</head><item><term>Biomedicine 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Language="En">Measurement of vitellogenin, a biomarker for exposure to oestrogenic chemicals, in a wide variety of cyprinid fish</ArticleTitle><ArticleCategory>Original Paper</ArticleCategory><ArticleFirstPage>418</ArticleFirstPage><ArticleLastPage>426</ArticleLastPage><ArticleHistory><RegistrationDate><Year>2006</Year><Month>2</Month><Day>18</Day></RegistrationDate><Accepted><Year>1996</Year><Month>6</Month><Day>8</Day></Accepted></ArticleHistory><ArticleCopyright><CopyrightHolderName>Springer-Verlag</CopyrightHolderName><CopyrightYear>1996</CopyrightYear></ArticleCopyright><ArticleGrants Type="Regular"><MetadataGrant Grant="OpenAccess"></MetadataGrant><AbstractGrant Grant="OpenAccess"></AbstractGrant><BodyPDFGrant Grant="Restricted"></BodyPDFGrant><BodyHTMLGrant Grant="Restricted"></BodyHTMLGrant><BibliographyGrant Grant="Restricted"></BibliographyGrant><ESMGrant Grant="Restricted"></ESMGrant></ArticleGrants><ArticleContext><JournalID>360</JournalID><VolumeIDStart>166</VolumeIDStart><VolumeIDEnd>166</VolumeIDEnd><IssueIDStart>7</IssueIDStart><IssueIDEnd>7</IssueIDEnd></ArticleContext></ArticleInfo><ArticleHeader><AuthorGroup><Author AffiliationIDS="Aff1" CorrespondingAffiliationID="Aff1"><AuthorName DisplayOrder="Western"><GivenName>C.</GivenName><GivenName>R.</GivenName><FamilyName>Tyler</FamilyName></AuthorName><Contact><Phone>+ 44-1895/274000</Phone><Fax>+ 44-1895/274348</Fax><Email>charles.tyler@brunel.ac.uk</Email></Contact></Author><Author AffiliationIDS="Aff1"><AuthorName DisplayOrder="Western"><GivenName>B.</GivenName><Particle>van der</Particle><FamilyName>Eerden</FamilyName></AuthorName></Author><Author AffiliationIDS="Aff1"><AuthorName DisplayOrder="Western"><GivenName>S.</GivenName><FamilyName>Jobling</FamilyName></AuthorName></Author><Author AffiliationIDS="Aff2"><AuthorName DisplayOrder="Western"><GivenName>G.</GivenName><FamilyName>Panter</FamilyName></AuthorName></Author><Author AffiliationIDS="Aff1"><AuthorName DisplayOrder="Western"><GivenName>J.</GivenName><GivenName>P.</GivenName><FamilyName>Sumpter</FamilyName></AuthorName></Author><Affiliation ID="Aff1"><OrgDivision>Department of Biology and Biochemistry</OrgDivision><OrgName>Brunel University</OrgName><OrgAddress><Postcode>UB8 3PH</Postcode><City>Uxbridge</City><State>Middlesex</State><Country>UK</Country></OrgAddress></Affiliation><Affiliation ID="Aff2"><OrgDivision>Zeneca, Brixham Environmental Laboratory</OrgDivision><OrgName>Freshwater Quarry</OrgName><OrgAddress><Postcode>1Q5 8BA</Postcode><City>Brixham</City><State>Devon</State><Country>UK</Country></OrgAddress></Affiliation></AuthorGroup><Abstract ID="Abs1" Language="En"><Heading>Abstract</Heading><Para>There is increasing concern about man-made chemicals in the aquatic environment that mimic oestrogens because they may disrupt reproductive function. Vitellogenin, a precursor of egg-yolk in fish and other oviparous animals, may be used as a biomarker for “oestrogen” exposure. This study investigated the use of a radioimmunoassay developed to carp (<Emphasis Type="Italic">Cyprinus carpio</Emphasis>) vitellogenin to measure vitellogenin in other species of fish, especially cyprinids that would be of value for field and laboratory studies on oestrogenic xenobiotics. Of the nine families of fish studied, only vitellogenin from cyprinids (to which the carp belongs) showed good cross-reactivity in the carp vitellogenin radioimmunoassay. Vitellogenin from cyprinids native to Europe that cross reacted in the carp vitellogenin radioimmunoassay included: bream (<Emphasis Type="Italic">Abramis brama</Emphasis>), roach (<Emphasis Type="Italic">Rutilus rutilus</Emphasis>), rudd (<Emphasis Type="Italic">Scardinius erythropthalmus</Emphasis>), gudgeon (<Emphasis Type="Italic">Gobio gobio</Emphasis>) and minnow (<Emphasis Type="Italic">Phoxinus phoxinus</Emphasis>). Vitellogenin from cyprinids used widely in ecotoxicology that cross reacted in the carp vitellogenin radioimmunoassay included: fathead minnow (<Emphasis Type="Italic">Pimephales promelas</Emphasis>), zebrafish (<Emphasis Type="Italic">Brachydanio rerio</Emphasis>) and goldfish (<Emphasis Type="Italic">Carassius auratus</Emphasis>). In the cyprinids studies, the concentrations of vitellogenin in mature females were between a few hundred and a thousand microgram per millilitre. Concentrations of plasma vitellogenin in immature females were always greater than 200 ng·m<Superscript>-1</Superscript>, whereas in males (with the exception of the fathead minnow) plasma vitellogenin concentrations were less than 20 ng·ml<Superscript>-1</Superscript> (and generally, much lower). The results suggest that the structure of vitellogenin is highly conserved within the cyprinid family and that the carp vitellogenin radioimmunoassay may be used to measure the concentrations of vitellogenin in plasma from a wide variety of cyprinids.</Para></Abstract><KeywordGroup Language="En"><Heading>Key words</Heading><Keyword>Oestrogenic</Keyword><Keyword>Vitellogenin</Keyword><Keyword>Cyprinids</Keyword><Keyword>Biomarker</Keyword><Keyword>Aquatic</Keyword></KeywordGroup><AbbreviationGroup><Heading>Abbreviations</Heading><DefinitionList><DefinitionListEntry><Term><Emphasis Type="Italic">PCBs</Emphasis></Term><Description><Para>polychlorinated biphenyls</Para></Description></DefinitionListEntry><DefinitionListEntry><Term><Emphasis Type="Italic">PAHs</Emphasis></Term><Description><Para>polycyclic aromatic hydrocarbons</Para></Description></DefinitionListEntry><DefinitionListEntry><Term><Emphasis Type="Italic">c-VTG</Emphasis></Term><Description><Para>carp vitellogenin</Para></Description></DefinitionListEntry><DefinitionListEntry><Term><Emphasis Type="Italic">GSI</Emphasis></Term><Description><Para>gonadosomatic index</Para></Description></DefinitionListEntry><DefinitionListEntry><Term><Emphasis Type="Italic">ELISA</Emphasis></Term><Description><Para>enzyme-linked immunosorbant assay</Para></Description></DefinitionListEntry><DefinitionListEntry><Term><Emphasis Type="Italic">VTG</Emphasis></Term><Description><Para>vitellogenin</Para></Description></DefinitionListEntry><DefinitionListEntry><Term><Emphasis Type="Italic">RJA</Emphasis></Term><Description><Para>radioimmunoassay</Para></Description></DefinitionListEntry><DefinitionListEntry><Term><Emphasis Type="Italic">STWs</Emphasis></Term><Description><Para>sewage treatment works</Para></Description></DefinitionListEntry></DefinitionList></AbbreviationGroup><ArticleNote Type="CommunicatedBy"><SimplePara>Communicated by H. Huddart</SimplePara></ArticleNote></ArticleHeader><NoBody></NoBody></Article></Issue></Volume></Journal></Publisher></istex:document></istex:metadataXml><mods version="3.6"><titleInfo lang="en"><title>Measurement of vitellogenin, a biomarker for exposure to oestrogenic chemicals, in a wide variety of cyprinid fish</title></titleInfo><titleInfo type="alternative" contentType="CDATA" lang="en"><title>Measurement of vitellogenin, a biomarker for exposure to oestrogenic chemicals, in a wide variety of cyprinid fish</title></titleInfo><name type="personal" displayLabel="corresp"><namePart type="given">C.</namePart><namePart type="given">R.</namePart><namePart type="family">Tyler</namePart><affiliation>Department of Biology and Biochemistry, Brunel University, UB8 3PH, Uxbridge, Middlesex, UK</affiliation><affiliation>E-mail: charles.tyler@brunel.ac.uk</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">B.</namePart><namePart type="family">van der Eerden</namePart><affiliation>Department of Biology and Biochemistry, Brunel University, UB8 3PH, Uxbridge, Middlesex, UK</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">S.</namePart><namePart type="family">Jobling</namePart><affiliation>Department of Biology and Biochemistry, Brunel University, UB8 3PH, Uxbridge, Middlesex, UK</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">G.</namePart><namePart type="family">Panter</namePart><affiliation>Zeneca, Brixham Environmental Laboratory, Freshwater Quarry, 1Q5 8BA, Brixham, Devon, UK</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">J.</namePart><namePart type="given">P.</namePart><namePart type="family">Sumpter</namePart><affiliation>Department of Biology and Biochemistry, Brunel University, UB8 3PH, Uxbridge, Middlesex, UK</affiliation><role><roleTerm type="text">author</roleTerm></role></name><typeOfResource>text</typeOfResource><genre type="research-article" displayLabel="OriginalPaper"></genre><originInfo><publisher>Springer-Verlag</publisher><place><placeTerm type="text">Berlin/Heidelberg</placeTerm></place><dateIssued encoding="w3cdtf">1996-11-01</dateIssued><copyrightDate encoding="w3cdtf">1996</copyrightDate></originInfo><language><languageTerm type="code" authority="rfc3066">en</languageTerm><languageTerm type="code" authority="iso639-2b">eng</languageTerm></language><physicalDescription><internetMediaType>text/html</internetMediaType></physicalDescription><abstract lang="en">Abstract: There is increasing concern about man-made chemicals in the aquatic environment that mimic oestrogens because they may disrupt reproductive function. Vitellogenin, a precursor of egg-yolk in fish and other oviparous animals, may be used as a biomarker for “oestrogen” exposure. This study investigated the use of a radioimmunoassay developed to carp (Cyprinus carpio) vitellogenin to measure vitellogenin in other species of fish, especially cyprinids that would be of value for field and laboratory studies on oestrogenic xenobiotics. Of the nine families of fish studied, only vitellogenin from cyprinids (to which the carp belongs) showed good cross-reactivity in the carp vitellogenin radioimmunoassay. Vitellogenin from cyprinids native to Europe that cross reacted in the carp vitellogenin radioimmunoassay included: bream (Abramis brama), roach (Rutilus rutilus), rudd (Scardinius erythropthalmus), gudgeon (Gobio gobio) and minnow (Phoxinus phoxinus). Vitellogenin from cyprinids used widely in ecotoxicology that cross reacted in the carp vitellogenin radioimmunoassay included: fathead minnow (Pimephales promelas), zebrafish (Brachydanio rerio) and goldfish (Carassius auratus). In the cyprinids studies, the concentrations of vitellogenin in mature females were between a few hundred and a thousand microgram per millilitre. Concentrations of plasma vitellogenin in immature females were always greater than 200 ng·m-1, whereas in males (with the exception of the fathead minnow) plasma vitellogenin concentrations were less than 20 ng·ml-1 (and generally, much lower). The results suggest that the structure of vitellogenin is highly conserved within the cyprinid family and that the carp vitellogenin radioimmunoassay may be used to measure the concentrations of vitellogenin in plasma from a wide variety of cyprinids.</abstract><note>Original Paper</note><subject><genre>Abbreviations</genre><topic> PCBs : polychlorinated biphenyls</topic><topic> PAHs : polycyclic aromatic hydrocarbons</topic><topic> c-VTG : carp vitellogenin</topic><topic> GSI : gonadosomatic index</topic><topic> ELISA : enzyme-linked immunosorbant assay</topic><topic> VTG : vitellogenin</topic><topic> RJA : radioimmunoassay</topic><topic> STWs : sewage treatment works</topic></subject><relatedItem type="host"><titleInfo><title>Journal of Comparative Physiology B</title><subTitle>Biochemical, Systemic and Environmental Physiology</subTitle></titleInfo><titleInfo type="abbreviated"><title>J Comp Physiol B</title></titleInfo><genre type="journal" displayLabel="Archive Journal"></genre><originInfo><dateIssued encoding="w3cdtf">1996-11-01</dateIssued><copyrightDate encoding="w3cdtf">1996</copyrightDate></originInfo><subject><genre>Life Sciences</genre><topic>Biomedicine general</topic><topic>Human Physiology</topic><topic>Biochemistry, general</topic><topic>Zoology</topic><topic>Animal Physiology</topic></subject><identifier type="ISSN">0174-1578</identifier><identifier type="eISSN">1432-136X</identifier><identifier type="JournalID">360</identifier><identifier type="IssueArticleCount">7</identifier><identifier type="VolumeIssueCount">8</identifier><part><date>1996</date><detail type="volume"><number>166</number><caption>vol.</caption></detail><detail type="issue"><number>7</number><caption>no.</caption></detail><extent unit="pages"><start>418</start><end>426</end></extent></part><recordInfo><recordOrigin>Springer-Verlag, 1996</recordOrigin></recordInfo></relatedItem><identifier type="istex">0785F1C3876172EA6B33ED793DF4E4620B9F16D5</identifier><identifier type="DOI">10.1007/BF02337886</identifier><identifier type="ArticleID">BF02337886</identifier><identifier type="ArticleID">Art3</identifier><accessCondition type="use and reproduction" contentType="copyright">Springer-Verlag, 1996</accessCondition><recordInfo><recordContentSource>SPRINGER</recordContentSource><recordOrigin>Springer-Verlag, 1996</recordOrigin></recordInfo></mods></metadata><serie></serie></istex></record>Pour manipuler ce document sous Unix (Dilib)
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