3D atlas describing the ontogenic evolution of the primary olfactory projections in the olfactory bulb of Xenopus laevis
Identifieur interne : 001291 ( Istex/Corpus ); précédent : 001290; suivant : 0012923D atlas describing the ontogenic evolution of the primary olfactory projections in the olfactory bulb of Xenopus laevis
Auteurs : Arnaud Gaudin ; Jean GascuelSource :
- Journal of Comparative Neurology [ 0021-9967 ] ; 2005-09-05.
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Abstract
The adult Xenopus presents the unique capability to smell odors both in water and air thanks to two different olfactory pathways. Nevertheless, the tadpole can initially perceive only water‐borne odorants, as the olfactory receptor neurons (ORN) that will detect air‐borne odorants develop later. Such a phenomenon requires major reorganization processes. Here we focused on the precise description of the neuroanatomical modifications occurring in the olfactory bulb (OB) of the tadpole throughout metamorphosis. Using both carbocyanine dyes and lectin staining, we investigated the evolution of ORN projection patterns into the OB from Stages 47 to 66, thus covering the period of time when all the modifications take place. Although our results confirm previous works (Reiss and Burd [1997] Semin Cell Dev Biol 8:171–179), we showed for the first time that the main olfactory bulb (MOB) is subdivided into seven zones at Stage 47 plus the accessory olfactory bulb (AOB). These seven zones receive fibers dedicated to aquatic olfaction (“aquatic fibers”) and are conserved until Stage 66. At Stage 48 the first fibers dedicated to the aerial olfaction constitute a new dorsomedial zone that grows steadily, pushing the seven original zones ventrolaterally. Only the part of the OB receiving aquatic fibers is fragmented, reminiscent of the organization described in fish. This raises the question of whether such an organization in zones constitutes a plesiomorphy or is linked to aquatic olfaction. We generated a 3D atlas at several stages which are representative of the reorganization process. This will be a useful tool for future studies of development and function. J. Comp. Neurol. 489:403–424, 2005. © 2005 Wiley‐Liss, Inc.
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DOI: 10.1002/cne.20655
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ISTEX:A1AE09DC88CB47FD9691FC872A9338473BFFF17FLe document en format XML
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At Stage 48 the first fibers dedicated to the aerial olfaction constitute a new dorsomedial zone that grows steadily, pushing the seven original zones ventrolaterally. Only the part of the OB receiving aquatic fibers is fragmented, reminiscent of the organization described in fish. This raises the question of whether such an organization in zones constitutes a plesiomorphy or is linked to aquatic olfaction. We generated a 3D atlas at several stages which are representative of the reorganization process. This will be a useful tool for future studies of development and function. J. Comp. 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At Stage 48 the first fibers dedicated to the aerial olfaction constitute a new dorsomedial zone that grows steadily, pushing the seven original zones ventrolaterally. Only the part of the OB receiving aquatic fibers is fragmented, reminiscent of the organization described in fish. This raises the question of whether such an organization in zones constitutes a plesiomorphy or is linked to aquatic olfaction. We generated a 3D atlas at several stages which are representative of the reorganization process. This will be a useful tool for future studies of development and function. J. Comp. 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Nevertheless, the tadpole can initially perceive only water‐borne odorants, as the olfactory receptor neurons (ORN) that will detect air‐borne odorants develop later. Such a phenomenon requires major reorganization processes. Here we focused on the precise description of the neuroanatomical modifications occurring in the olfactory bulb (OB) of the tadpole throughout metamorphosis. Using both carbocyanine dyes and lectin staining, we investigated the evolution of ORN projection patterns into the OB from Stages 47 to 66, thus covering the period of time when all the modifications take place. Although our results confirm previous works (Reiss and Burd [1997] Semin Cell Dev Biol 8:171–179), we showed for the first time that the main olfactory bulb (MOB) is subdivided into seven zones at Stage 47 plus the accessory olfactory bulb (AOB). These seven zones receive fibers dedicated to aquatic olfaction (“aquatic fibers”) and are conserved until Stage 66. At Stage 48 the first fibers dedicated to the aerial olfaction constitute a new dorsomedial zone that grows steadily, pushing the seven original zones ventrolaterally. Only the part of the OB receiving aquatic fibers is fragmented, reminiscent of the organization described in fish. This raises the question of whether such an organization in zones constitutes a plesiomorphy or is linked to aquatic olfaction. We generated a 3D atlas at several stages which are representative of the reorganization process. This will be a useful tool for future studies of development and function. J. Comp. Neurol. 489:403–424, 2005. © 2005 Wiley‐Liss, Inc.</p></abstract></abstractGroup></contentMeta></header></component></istex:document></istex:metadataXml><mods version="3.6"><titleInfo lang="en"><title>3D atlas describing the ontogenic evolution of the primary olfactory projections in the olfactory bulb of Xenopus laevis</title></titleInfo><titleInfo type="abbreviated" lang="en"><title>3D Atlas of Segmentation of Olfactory Bulb</title></titleInfo><titleInfo type="alternative" contentType="CDATA" lang="en"><title>3D atlas describing the ontogenic evolution of the primary olfactory projections in the olfactory bulb of Xenopus laevis</title></titleInfo><name type="personal"><namePart type="given">Arnaud</namePart><namePart type="family">Gaudin</namePart><affiliation>Centre des Sciences du Goût (Unité Mixte de Recherche 5170 Centre National de la Recherche Scientifique–Université de Bourgogne–Institut National de la Recherche Agronomique), F‐21000 Dijon, France</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">Jean</namePart><namePart type="family">Gascuel</namePart><affiliation>Centre des Sciences du Goût (Unité Mixte de Recherche 5170 Centre National de la Recherche Scientifique–Université de Bourgogne–Institut National de la Recherche Agronomique), F‐21000 Dijon, France</affiliation><affiliation>Centre des Sciences du Goût (UMR 5170 CNRS‐Université de Bourgogne‐INRA), 15 rue Hugues Picardet, F‐21000 Dijon, France</affiliation><role><roleTerm type="text">author</roleTerm></role></name><typeOfResource>text</typeOfResource><genre type="article" displayLabel="article"></genre><originInfo><publisher>Wiley Subscription Services, Inc., A Wiley Company</publisher><place><placeTerm type="text">Hoboken</placeTerm></place><dateIssued encoding="w3cdtf">2005-09-05</dateIssued><dateCaptured encoding="w3cdtf">2004-09-24</dateCaptured><dateValid encoding="w3cdtf">2005-03-29</dateValid><copyrightDate encoding="w3cdtf">2005</copyrightDate></originInfo><language><languageTerm type="code" authority="rfc3066">en</languageTerm><languageTerm type="code" authority="iso639-2b">eng</languageTerm></language><physicalDescription><internetMediaType>text/html</internetMediaType><extent unit="figures">9</extent><extent unit="tables">1</extent><extent unit="references">89</extent><extent unit="words">16875</extent></physicalDescription><abstract lang="en">The adult Xenopus presents the unique capability to smell odors both in water and air thanks to two different olfactory pathways. Nevertheless, the tadpole can initially perceive only water‐borne odorants, as the olfactory receptor neurons (ORN) that will detect air‐borne odorants develop later. Such a phenomenon requires major reorganization processes. Here we focused on the precise description of the neuroanatomical modifications occurring in the olfactory bulb (OB) of the tadpole throughout metamorphosis. Using both carbocyanine dyes and lectin staining, we investigated the evolution of ORN projection patterns into the OB from Stages 47 to 66, thus covering the period of time when all the modifications take place. Although our results confirm previous works (Reiss and Burd [1997] Semin Cell Dev Biol 8:171–179), we showed for the first time that the main olfactory bulb (MOB) is subdivided into seven zones at Stage 47 plus the accessory olfactory bulb (AOB). These seven zones receive fibers dedicated to aquatic olfaction (“aquatic fibers”) and are conserved until Stage 66. At Stage 48 the first fibers dedicated to the aerial olfaction constitute a new dorsomedial zone that grows steadily, pushing the seven original zones ventrolaterally. Only the part of the OB receiving aquatic fibers is fragmented, reminiscent of the organization described in fish. This raises the question of whether such an organization in zones constitutes a plesiomorphy or is linked to aquatic olfaction. We generated a 3D atlas at several stages which are representative of the reorganization process. This will be a useful tool for future studies of development and function. J. Comp. Neurol. 489:403–424, 2005. © 2005 Wiley‐Liss, Inc.</abstract><note type="funding">Burgundy council–Institut National de la Recherche Agronomique - No. BTH01150; </note><subject lang="en"><genre>keywords</genre><topic>olfactory receptor neurons</topic><topic>olfactory bulb</topic><topic>neuroanatomy</topic><topic>developmental biology</topic><topic>olfaction</topic><topic>confocal microscopy</topic><topic>3D imaging</topic></subject><relatedItem type="host"><titleInfo><title>Journal of Comparative Neurology</title></titleInfo><titleInfo type="abbreviated"><title>J. Comp. Neurol.</title></titleInfo><genre type="journal">journal</genre><note type="content"> This article includes Supplementary Material available via the internet at http://www.interscience.wiley.com/jpages/xxxx‐xxxx/suppmat .Supporting Info Item: 3‐D animations and 3‐D panels: representative stages 47, 50, 59, 61, 66 - Description of representative stages - </note><subject><genre>article-category</genre><topic>Article</topic></subject><identifier type="ISSN">0021-9967</identifier><identifier type="eISSN">1096-9861</identifier><identifier type="DOI">10.1002/(ISSN)1096-9861</identifier><identifier type="PublisherID">CNE</identifier><part><date>2005</date><detail type="volume"><caption>vol.</caption><number>489</number></detail><detail type="issue"><caption>no.</caption><number>4</number></detail><extent unit="pages"><start>403</start><end>424</end><total>22</total></extent></part></relatedItem><identifier type="istex">A1AE09DC88CB47FD9691FC872A9338473BFFF17F</identifier><identifier type="DOI">10.1002/cne.20655</identifier><identifier type="ArticleID">CNE20655</identifier><accessCondition type="use and reproduction" contentType="copyright">Copyright © 2005 Wiley‐Liss, Inc.</accessCondition><recordInfo><recordContentSource>WILEY</recordContentSource><recordOrigin>Wiley Subscription Services, Inc., A Wiley Company</recordOrigin></recordInfo></mods></metadata><serie></serie></istex></record>Pour manipuler ce document sous Unix (Dilib)
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