Multigenerational exposure of the estuarine sheepshead minnow (Cyprinodon variegatus) to 17β‐estradiol. I. Organism‐level effects over three generations
Identifieur interne : 001282 ( Istex/Corpus ); précédent : 001281; suivant : 001283Multigenerational exposure of the estuarine sheepshead minnow (Cyprinodon variegatus) to 17β‐estradiol. I. Organism‐level effects over three generations
Auteurs : Geraldine M. Cripe ; Becky L. Hemmer ; Larry R. Goodman ; John W. Fournie ; Sandy Raimondo ; Joann C. Vennari ; Rodney L. Danner ; Kent Smith ; Blaze R. Manfredonia ; Dannielle H. Kulaw ; Michael J. HemmerSource :
- Environmental Toxicology and Chemistry [ 0730-7268 ] ; 2009-11.
English descriptors
Abstract
A 280‐d study examined the effects of 17β‐estradiol (E2) on reproduction and development of the sheepshead minnow (Cyprinodon variegatus) exposed from the parental (F0) through three subsequent (F1, F2, and F3) generations and evaluated the need for multigenerational assessments of the risks of endocrine‐disrupting chemicals. This first three‐generation study exposed adult F0 and F1 fish to measured concentrations of 0.01, 0.04, 0.08, 0.2, and 0.3 μg E2/L; the F2 and F3 generations were exposed to 0.2 μg E2/L or less. The cumulative 21‐d production of normal embryos was significantly reduced in the F0 generation at 0.3 μg E2/L and in the F1 and F2 generations at 0.08 μg E2/L or more. The daily reproductive rate was significantly reduced in all three generations at 0.08 μg E2/L or more during spawning days 8 to 14 and 15 to 21. The proportion of infertile eggs from F1 fish was significantly increased above that of the solvent controls at 0.04 and 0.2 μg E2/L and from F2 fish at 0.04 μg E2/L or more. Changes in liver, kidney, and gonadal tissues were seen in the F0 and F1 generations exposed to 0.2 μg E2/L or more. The female gonadosomatic index was significantly decreased at 0.3 μg E2/L in the F0 and F1 generations. Estradiol affected the hepatosomatic index only in female F1 fish, but not in a dose‐dependent manner. All F1 fish in 0.3 μg E2/L appeared to be phenotypically female. Our results indicate that life‐cycle exposure to E2 significantly decreased embryo production by F1 and F2 fish at concentrations lower than those affecting the F0 generation, and they emphasize the importance of evaluating the impact of an estrogenic chemical on reproduction through a minimum of two (F0 and F1) generations.
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DOI: 10.1897/08-542.1
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Organism‐level effects over three generations</title><author><name sortKey="Cripe, Geraldine M" sort="Cripe, Geraldine M" uniqKey="Cripe G" first="Geraldine M." last="Cripe">Geraldine M. Cripe</name><affiliation><mods:affiliation>U.S. Environmental Protection Agency, Office of Research and Development, National Health and Environmental Effects Research Laboratory, Gulf Ecology Division, Gulf Breeze, Florida 32561</mods:affiliation></affiliation></author><author><name sortKey="Hemmer, Becky L" sort="Hemmer, Becky L" uniqKey="Hemmer B" first="Becky L." last="Hemmer">Becky L. Hemmer</name><affiliation><mods:affiliation>U.S. Environmental Protection Agency, Office of Research and Development, National Health and Environmental Effects Research Laboratory, Gulf Ecology Division, Gulf Breeze, Florida 32561</mods:affiliation></affiliation></author><author><name sortKey="Goodman, Larry R" sort="Goodman, Larry R" uniqKey="Goodman L" first="Larry R." last="Goodman">Larry R. 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Vennari</name><affiliation><mods:affiliation>Senior Services of America, Silver Spring, Maryland 20910, USA</mods:affiliation></affiliation></author><author><name sortKey="Danner, Rodney L" sort="Danner, Rodney L" uniqKey="Danner R" first="Rodney L." last="Danner">Rodney L. Danner</name><affiliation><mods:affiliation>Senior Services of America, Silver Spring, Maryland 20910, USA</mods:affiliation></affiliation></author><author><name sortKey="Smith, Kent" sort="Smith, Kent" uniqKey="Smith K" first="Kent" last="Smith">Kent Smith</name><affiliation><mods:affiliation>Senior Services of America, Silver Spring, Maryland 20910, USA</mods:affiliation></affiliation></author><author><name sortKey="Manfredonia, Blaze R" sort="Manfredonia, Blaze R" uniqKey="Manfredonia B" first="Blaze R." last="Manfredonia">Blaze R. 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This first three‐generation study exposed adult F0 and F1 fish to measured concentrations of 0.01, 0.04, 0.08, 0.2, and 0.3 μg E2/L; the F2 and F3 generations were exposed to 0.2 μg E2/L or less. The cumulative 21‐d production of normal embryos was significantly reduced in the F0 generation at 0.3 μg E2/L and in the F1 and F2 generations at 0.08 μg E2/L or more. The daily reproductive rate was significantly reduced in all three generations at 0.08 μg E2/L or more during spawning days 8 to 14 and 15 to 21. The proportion of infertile eggs from F1 fish was significantly increased above that of the solvent controls at 0.04 and 0.2 μg E2/L and from F2 fish at 0.04 μg E2/L or more. Changes in liver, kidney, and gonadal tissues were seen in the F0 and F1 generations exposed to 0.2 μg E2/L or more. The female gonadosomatic index was significantly decreased at 0.3 μg E2/L in the F0 and F1 generations. Estradiol affected the hepatosomatic index only in female F1 fish, but not in a dose‐dependent manner. All F1 fish in 0.3 μg E2/L appeared to be phenotypically female. Our results indicate that life‐cycle exposure to E2 significantly decreased embryo production by F1 and F2 fish at concentrations lower than those affecting the F0 generation, and they emphasize the importance of evaluating the impact of an estrogenic chemical on reproduction through a minimum of two (F0 and F1) generations.</div></front></TEI><istex><corpusName>wiley</corpusName><author><json:item><name>Geraldine M. Cripe</name><affiliations><json:string>U.S. Environmental Protection Agency, Office of Research and Development, National Health and Environmental Effects Research Laboratory, Gulf Ecology Division, Gulf Breeze, Florida 32561</json:string></affiliations></json:item><json:item><name>Becky L. 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Fournie</name><affiliations><json:string>U.S. Environmental Protection Agency, Office of Research and Development, National Health and Environmental Effects Research Laboratory, Gulf Ecology Division, Gulf Breeze, Florida 32561</json:string></affiliations></json:item><json:item><name>Sandy Raimondo</name><affiliations><json:string>U.S. Environmental Protection Agency, Office of Research and Development, National Health and Environmental Effects Research Laboratory, Gulf Ecology Division, Gulf Breeze, Florida 32561</json:string><json:string>U.S. Environmental Protection Agency, Office of Research and Development, National Health and Environmental Effects Research Laboratory, Gulf Ecology Division, Gulf Breeze, Florida 32561</json:string></affiliations></json:item><json:item><name>Joann C. Vennari</name><affiliations><json:string>Senior Services of America, Silver Spring, Maryland 20910, USA</json:string></affiliations></json:item><json:item><name>Rodney L. Danner</name><affiliations><json:string>Senior Services of America, Silver Spring, Maryland 20910, USA</json:string></affiliations></json:item><json:item><name>Kent Smith</name><affiliations><json:string>Senior Services of America, Silver Spring, Maryland 20910, USA</json:string></affiliations></json:item><json:item><name>Blaze R. Manfredonia</name><affiliations><json:string>Senior Services of America, Silver Spring, Maryland 20910, USA</json:string></affiliations></json:item><json:item><name>Dannielle H. Kulaw</name><affiliations><json:string>Student Services Contractor, Ft. Walton Beach, Florida 32547, USA</json:string></affiliations></json:item><json:item><name>Michael J. Hemmer</name><affiliations><json:string>U.S. Environmental Protection Agency, Office of Research and Development, National Health and Environmental Effects Research Laboratory, Gulf Ecology Division, Gulf Breeze, Florida 32561</json:string></affiliations></json:item></author><subject><json:item><lang><json:string>eng</json:string></lang><value>17β‐Estradiol</value></json:item><json:item><lang><json:string>eng</json:string></lang><value>Sheepshead minnow</value></json:item><json:item><lang><json:string>eng</json:string></lang><value>Reproduction</value></json:item><json:item><lang><json:string>eng</json:string></lang><value>Development</value></json:item><json:item><lang><json:string>eng</json:string></lang><value>Multiple generation</value></json:item></subject><articleId><json:string>ETC5620281119</json:string></articleId><language><json:string>eng</json:string></language><originalGenre><json:string>article</json:string></originalGenre><abstract>A 280‐d study examined the effects of 17β‐estradiol (E2) on reproduction and development of the sheepshead minnow (Cyprinodon variegatus) exposed from the parental (F0) through three subsequent (F1, F2, and F3) generations and evaluated the need for multigenerational assessments of the risks of endocrine‐disrupting chemicals. This first three‐generation study exposed adult F0 and F1 fish to measured concentrations of 0.01, 0.04, 0.08, 0.2, and 0.3 μg E2/L; the F2 and F3 generations were exposed to 0.2 μg E2/L or less. The cumulative 21‐d production of normal embryos was significantly reduced in the F0 generation at 0.3 μg E2/L and in the F1 and F2 generations at 0.08 μg E2/L or more. The daily reproductive rate was significantly reduced in all three generations at 0.08 μg E2/L or more during spawning days 8 to 14 and 15 to 21. The proportion of infertile eggs from F1 fish was significantly increased above that of the solvent controls at 0.04 and 0.2 μg E2/L and from F2 fish at 0.04 μg E2/L or more. Changes in liver, kidney, and gonadal tissues were seen in the F0 and F1 generations exposed to 0.2 μg E2/L or more. The female gonadosomatic index was significantly decreased at 0.3 μg E2/L in the F0 and F1 generations. Estradiol affected the hepatosomatic index only in female F1 fish, but not in a dose‐dependent manner. All F1 fish in 0.3 μg E2/L appeared to be phenotypically female. Our results indicate that life‐cycle exposure to E2 significantly decreased embryo production by F1 and F2 fish at concentrations lower than those affecting the F0 generation, and they emphasize the importance of evaluating the impact of an estrogenic chemical on reproduction through a minimum of two (F0 and F1) generations.</abstract><qualityIndicators><score>8</score><pdfVersion>1.3</pdfVersion><pdfPageSize>594 x 792 pts</pdfPageSize><refBibsNative>true</refBibsNative><abstractCharCount>1729</abstractCharCount><pdfWordCount>9154</pdfWordCount><pdfCharCount>57202</pdfCharCount><pdfPageCount>12</pdfPageCount><abstractWordCount>288</abstractWordCount></qualityIndicators><title>Multigenerational exposure of the estuarine sheepshead minnow (Cyprinodon variegatus) to 17β‐estradiol. I. 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I. Organism‐level effects over three generations</title></titleStmt><publicationStmt><authority>ISTEX</authority><publisher>Wiley Periodicals, Inc.</publisher><pubPlace>Hoboken</pubPlace><availability><p>Copyright © 2009 SETAC</p></availability><date>2009</date></publicationStmt><notesStmt><note type="content">*This is contribution 1343 from the Gulf Ecology Division.</note></notesStmt><sourceDesc><biblStruct type="inbook"><analytic><title level="a" type="main" xml:lang="en">Multigenerational exposure of the estuarine sheepshead minnow (Cyprinodon variegatus) to 17β‐estradiol. I. Organism‐level effects over three generations</title><author xml:id="author-1"><persName><forename type="first">Geraldine M.</forename><surname>Cripe</surname></persName><affiliation>U.S. Environmental Protection Agency, Office of Research and Development, National Health and Environmental Effects Research Laboratory, Gulf Ecology Division, Gulf Breeze, Florida 32561</affiliation></author><author xml:id="author-2"><persName><forename type="first">Becky L.</forename><surname>Hemmer</surname></persName><affiliation>U.S. Environmental Protection Agency, Office of Research and Development, National Health and Environmental Effects Research Laboratory, Gulf Ecology Division, Gulf Breeze, Florida 32561</affiliation></author><author xml:id="author-3"><persName><forename type="first">Larry R.</forename><surname>Goodman</surname></persName><affiliation>U.S. Environmental Protection Agency, Office of Research and Development, National Health and Environmental Effects Research Laboratory, Gulf Ecology Division, Gulf Breeze, Florida 32561</affiliation></author><author xml:id="author-4"><persName><forename type="first">John W.</forename><surname>Fournie</surname></persName><affiliation>U.S. Environmental Protection Agency, Office of Research and Development, National Health and Environmental Effects Research Laboratory, Gulf Ecology Division, Gulf Breeze, Florida 32561</affiliation></author><author xml:id="author-5"><persName><forename type="first">Sandy</forename><surname>Raimondo</surname></persName><affiliation>U.S. Environmental Protection Agency, Office of Research and Development, National Health and Environmental Effects Research Laboratory, Gulf Ecology Division, Gulf Breeze, Florida 32561</affiliation><affiliation>U.S. Environmental Protection Agency, Office of Research and Development, National Health and Environmental Effects Research Laboratory, Gulf Ecology Division, Gulf Breeze, Florida 32561</affiliation></author><author xml:id="author-6"><persName><forename type="first">Joann C.</forename><surname>Vennari</surname></persName><affiliation>Senior Services of America, Silver Spring, Maryland 20910, USA</affiliation></author><author xml:id="author-7"><persName><forename type="first">Rodney L.</forename><surname>Danner</surname></persName><affiliation>Senior Services of America, Silver Spring, Maryland 20910, USA</affiliation></author><author xml:id="author-8"><persName><forename type="first">Kent</forename><surname>Smith</surname></persName><affiliation>Senior Services of America, Silver Spring, Maryland 20910, USA</affiliation></author><author xml:id="author-9"><persName><forename type="first">Blaze R.</forename><surname>Manfredonia</surname></persName><affiliation>Senior Services of America, Silver Spring, Maryland 20910, USA</affiliation></author><author xml:id="author-10"><persName><forename type="first">Dannielle H.</forename><surname>Kulaw</surname></persName><affiliation>Student Services Contractor, Ft. Walton Beach, Florida 32547, USA</affiliation></author><author xml:id="author-11"><persName><forename type="first">Michael J.</forename><surname>Hemmer</surname></persName><affiliation>U.S. Environmental Protection Agency, Office of Research and Development, National Health and Environmental Effects Research Laboratory, Gulf Ecology Division, Gulf Breeze, Florida 32561</affiliation></author></analytic><monogr><title level="j">Environmental Toxicology and Chemistry</title><title level="j" type="sub">An International Journal</title><title level="j" type="abbrev">Environmental Toxicology and Chemistry</title><idno type="pISSN">0730-7268</idno><idno type="eISSN">1552-8618</idno><idno type="DOI">10.1002/(ISSN)1552-8618</idno><imprint><publisher>Wiley Periodicals, Inc.</publisher><pubPlace>Hoboken</pubPlace><date type="published" when="2009-11"></date><biblScope unit="volume">28</biblScope><biblScope unit="issue">11</biblScope><biblScope unit="page" from="2397">2397</biblScope><biblScope unit="page" to="2408">2408</biblScope></imprint></monogr><idno type="istex">13674B4245E1E37829A90CA740493C31BB56821A</idno><idno type="DOI">10.1897/08-542.1</idno><idno type="ArticleID">ETC5620281119</idno></biblStruct></sourceDesc></fileDesc><profileDesc><creation><date>2009</date></creation><langUsage><language ident="en">en</language></langUsage><abstract xml:lang="en"><p>A 280‐d study examined the effects of 17β‐estradiol (E2) on reproduction and development of the sheepshead minnow (Cyprinodon variegatus) exposed from the parental (F0) through three subsequent (F1, F2, and F3) generations and evaluated the need for multigenerational assessments of the risks of endocrine‐disrupting chemicals. This first three‐generation study exposed adult F0 and F1 fish to measured concentrations of 0.01, 0.04, 0.08, 0.2, and 0.3 μg E2/L; the F2 and F3 generations were exposed to 0.2 μg E2/L or less. The cumulative 21‐d production of normal embryos was significantly reduced in the F0 generation at 0.3 μg E2/L and in the F1 and F2 generations at 0.08 μg E2/L or more. The daily reproductive rate was significantly reduced in all three generations at 0.08 μg E2/L or more during spawning days 8 to 14 and 15 to 21. The proportion of infertile eggs from F1 fish was significantly increased above that of the solvent controls at 0.04 and 0.2 μg E2/L and from F2 fish at 0.04 μg E2/L or more. Changes in liver, kidney, and gonadal tissues were seen in the F0 and F1 generations exposed to 0.2 μg E2/L or more. The female gonadosomatic index was significantly decreased at 0.3 μg E2/L in the F0 and F1 generations. Estradiol affected the hepatosomatic index only in female F1 fish, but not in a dose‐dependent manner. All F1 fish in 0.3 μg E2/L appeared to be phenotypically female. Our results indicate that life‐cycle exposure to E2 significantly decreased embryo production by F1 and F2 fish at concentrations lower than those affecting the F0 generation, and they emphasize the importance of evaluating the impact of an estrogenic chemical on reproduction through a minimum of two (F0 and F1) generations.</p></abstract><textClass xml:lang="en"><keywords scheme="keyword"><list><head>keywords</head><item><term>17β‐Estradiol</term></item><item><term>Sheepshead minnow</term></item><item><term>Reproduction</term></item><item><term>Development</term></item><item><term>Multiple generation</term></item></list></keywords></textClass><textClass><keywords scheme="Journal Subject"><list><head>article-category</head><item><term>Environmental Toxicology</term></item></list></keywords></textClass></profileDesc><revisionDesc><change when="2008-10-28">Received</change><change when="2009-06-10">Registration</change><change when="2009-11">Published</change></revisionDesc></teiHeader></istex:fulltextTEI><json:item><extension>txt</extension><original>false</original><mimetype>text/plain</mimetype><uri>https://api.istex.fr/document/13674B4245E1E37829A90CA740493C31BB56821A/fulltext/txt</uri></json:item></fulltext><metadata><istex:metadataXml wicri:clean="Wiley, elements deleted: body"><istex:xmlDeclaration>version="1.0" encoding="UTF-8" standalone="yes"</istex:xmlDeclaration><istex:document><component version="2.0" type="serialArticle" xml:lang="en"><header><publicationMeta level="product"><publisherInfo><publisherName>Wiley Periodicals, Inc.</publisherName><publisherLoc>Hoboken</publisherLoc></publisherInfo><doi registered="yes">10.1002/(ISSN)1552-8618</doi><issn type="print">0730-7268</issn><issn type="electronic">1552-8618</issn><idGroup><id type="product" value="ETC"></id></idGroup><titleGroup><title type="main" xml:lang="en" sort="ENVIRONMENTAL TOXICOLOGY AND CHEMISTRY">Environmental Toxicology and Chemistry</title><title type="subtitle">An International Journal</title><title type="short">Environmental Toxicology and Chemistry</title></titleGroup></publicationMeta><publicationMeta level="part" position="110"><doi origin="wiley" registered="no">10.1002/etc.v28:11</doi><numberingGroup><numbering type="journalVolume" number="28">28</numbering><numbering type="journalIssue">11</numbering></numberingGroup><coverDate startDate="2009-11">November 2009</coverDate></publicationMeta><publicationMeta level="unit" type="article" position="19" status="forIssue"><doi origin="wiley" registered="yes">10.1897/08-542.1</doi><idGroup><id type="unit" value="ETC5620281119"></id></idGroup><countGroup><count type="pageTotal" number="12"></count></countGroup><titleGroup><title type="articleCategory">Environmental Toxicology</title><title type="tocHeading1">Environmental Toxicology</title></titleGroup><copyright ownership="thirdParty">Copyright © 2009 SETAC</copyright><eventGroup><event type="manuscriptReceived" date="2008-10-28"></event><event type="manuscriptAccepted" date="2009-06-10"></event><event type="firstOnline" date="2010-01-06"></event><event type="publishedOnlineFinalForm" date="2010-01-06"></event><event type="xmlConverted" agent="Converter:JWSART34_TO_WML3G version:2.3.2 mode:FullText source:FullText result:FullText" date="2010-03-15"></event><event type="xmlConverted" agent="Converter:WILEY_ML3G_TO_WILEY_ML3GV2 version:3.8.8" date="2014-01-25"></event><event type="xmlConverted" agent="Converter:WML3G_To_WML3G version:4.1.7 mode:FullText,remove_FC" date="2014-10-16"></event></eventGroup><numberingGroup><numbering type="pageFirst">2397</numbering><numbering type="pageLast">2408</numbering></numberingGroup><correspondenceTo>U.S. Environmental Protection Agency, Office of Research and Development, National Health and Environmental Effects Research Laboratory, Gulf Ecology Division, Gulf Breeze, Florida 32561</correspondenceTo><linkGroup><link type="toTypesetVersion" href="file:ETC.ETC5620281119.pdf"></link></linkGroup></publicationMeta><contentMeta><countGroup><count type="figureTotal" number="6"></count><count type="tableTotal" number="2"></count><count type="referenceTotal" number="46"></count></countGroup><titleGroup><title type="main" xml:lang="en">Multigenerational exposure of the estuarine sheepshead minnow (<i>Cyprinodon variegatus</i>) to 17β‐estradiol. I. Organism‐level effects over three generations<link href="#fn1"></link></title><title type="short" xml:lang="en">Estradiol effects on three generations of sheepshead minnows</title></titleGroup><creators><creator xml:id="au1" creatorRole="author" affiliationRef="#af1"><personName><givenNames>Geraldine M.</givenNames><familyName>Cripe</familyName></personName></creator><creator xml:id="au2" creatorRole="author" affiliationRef="#af1"><personName><givenNames>Becky L.</givenNames><familyName>Hemmer</familyName></personName></creator><creator xml:id="au3" creatorRole="author" affiliationRef="#af1"><personName><givenNames>Larry R.</givenNames><familyName>Goodman</familyName></personName></creator><creator xml:id="au4" creatorRole="author" affiliationRef="#af1"><personName><givenNames>John W.</givenNames><familyName>Fournie</familyName></personName></creator><creator xml:id="au5" creatorRole="author" affiliationRef="#af1" corresponding="yes"><personName><givenNames>Sandy</givenNames><familyName>Raimondo</familyName></personName><contactDetails><email>raimondo.sandy@epa.gov</email></contactDetails></creator><creator xml:id="au6" creatorRole="author" affiliationRef="#af2"><personName><givenNames>Joann C.</givenNames><familyName>Vennari</familyName></personName></creator><creator xml:id="au7" creatorRole="author" affiliationRef="#af2"><personName><givenNames>Rodney L.</givenNames><familyName>Danner</familyName></personName></creator><creator xml:id="au8" creatorRole="author" affiliationRef="#af2"><personName><givenNames>Kent</givenNames><familyName>Smith</familyName></personName></creator><creator xml:id="au9" creatorRole="author" affiliationRef="#af2"><personName><givenNames>Blaze R.</givenNames><familyName>Manfredonia</familyName></personName></creator><creator xml:id="au10" creatorRole="author" affiliationRef="#af3"><personName><givenNames>Dannielle H.</givenNames><familyName>Kulaw</familyName></personName></creator><creator xml:id="au11" creatorRole="author" affiliationRef="#af1"><personName><givenNames>Michael J.</givenNames><familyName>Hemmer</familyName></personName></creator></creators><affiliationGroup><affiliation xml:id="af1" countryCode="US" type="organization"><unparsedAffiliation>U.S. Environmental Protection Agency, Office of Research and Development, National Health and Environmental Effects Research Laboratory, Gulf Ecology Division, Gulf Breeze, Florida 32561</unparsedAffiliation></affiliation><affiliation xml:id="af2" countryCode="US" type="organization"><unparsedAffiliation>Senior Services of America, Silver Spring, Maryland 20910, USA</unparsedAffiliation></affiliation><affiliation xml:id="af3" countryCode="US" type="organization"><unparsedAffiliation>Student Services Contractor, Ft. Walton Beach, Florida 32547, USA</unparsedAffiliation></affiliation></affiliationGroup><keywordGroup xml:lang="en" type="author"><keyword xml:id="kwd1">17β‐Estradiol</keyword><keyword xml:id="kwd2">Sheepshead minnow</keyword><keyword xml:id="kwd3">Reproduction</keyword><keyword xml:id="kwd4">Development</keyword><keyword xml:id="kwd5">Multiple generation</keyword></keywordGroup><abstractGroup><abstract type="main" xml:lang="en"><title type="main">Abstract</title><p>A 280‐d study examined the effects of 17β‐estradiol (E2) on reproduction and development of the sheepshead minnow (<i>Cyprinodon variegatus</i>) exposed from the parental (F0) through three subsequent (F1, F2, and F3) generations and evaluated the need for multigenerational assessments of the risks of endocrine‐disrupting chemicals. This first three‐generation study exposed adult F0 and F1 fish to measured concentrations of 0.01, 0.04, 0.08, 0.2, and 0.3 μg E2/L; the F2 and F3 generations were exposed to 0.2 μg E2/L or less. The cumulative 21‐d production of normal embryos was significantly reduced in the F0 generation at 0.3 μg E2/L and in the F1 and F2 generations at 0.08 μg E2/L or more. The daily reproductive rate was significantly reduced in all three generations at 0.08 μg E2/L or more during spawning days 8 to 14 and 15 to 21. The proportion of infertile eggs from F1 fish was significantly increased above that of the solvent controls at 0.04 and 0.2 μg E2/L and from F2 fish at 0.04 μg E2/L or more. Changes in liver, kidney, and gonadal tissues were seen in the F0 and F1 generations exposed to 0.2 μg E2/L or more. The female gonadosomatic index was significantly decreased at 0.3 μg E2/L in the F0 and F1 generations. Estradiol affected the hepatosomatic index only in female F1 fish, but not in a dose‐dependent manner. All F1 fish in 0.3 μg E2/L appeared to be phenotypically female. Our results indicate that life‐cycle exposure to E2 significantly decreased embryo production by F1 and F2 fish at concentrations lower than those affecting the F0 generation, and they emphasize the importance of evaluating the impact of an estrogenic chemical on reproduction through a minimum of two (F0 and F1) generations.</p></abstract></abstractGroup></contentMeta><noteGroup><note xml:id="fn1"><p>This is contribution 1343 from the Gulf Ecology Division.</p></note></noteGroup></header></component></istex:document></istex:metadataXml><mods version="3.6"><titleInfo lang="en"><title>Multigenerational exposure of the estuarine sheepshead minnow (Cyprinodon variegatus) to 17β‐estradiol. I. Organism‐level effects over three generations</title></titleInfo><titleInfo type="abbreviated" lang="en"><title>Estradiol effects on three generations of sheepshead minnows</title></titleInfo><titleInfo type="alternative" contentType="CDATA" lang="en"><title>Multigenerational exposure of the estuarine sheepshead minnow (Cyprinodon variegatus) to 17β‐estradiol. I. Organism‐level effects over three generations</title></titleInfo><name type="personal"><namePart type="given">Geraldine M.</namePart><namePart type="family">Cripe</namePart><affiliation>U.S. Environmental Protection Agency, Office of Research and Development, National Health and Environmental Effects Research Laboratory, Gulf Ecology Division, Gulf Breeze, Florida 32561</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">Becky L.</namePart><namePart type="family">Hemmer</namePart><affiliation>U.S. Environmental Protection Agency, Office of Research and Development, National Health and Environmental Effects Research Laboratory, Gulf Ecology Division, Gulf Breeze, Florida 32561</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">Larry R.</namePart><namePart type="family">Goodman</namePart><affiliation>U.S. Environmental Protection Agency, Office of Research and Development, National Health and Environmental Effects Research Laboratory, Gulf Ecology Division, Gulf Breeze, Florida 32561</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">John W.</namePart><namePart type="family">Fournie</namePart><affiliation>U.S. Environmental Protection Agency, Office of Research and Development, National Health and Environmental Effects Research Laboratory, Gulf Ecology Division, Gulf Breeze, Florida 32561</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">Sandy</namePart><namePart type="family">Raimondo</namePart><affiliation>U.S. Environmental Protection Agency, Office of Research and Development, National Health and Environmental Effects Research Laboratory, Gulf Ecology Division, Gulf Breeze, Florida 32561</affiliation><affiliation>U.S. Environmental Protection Agency, Office of Research and Development, National Health and Environmental Effects Research Laboratory, Gulf Ecology Division, Gulf Breeze, Florida 32561</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">Joann C.</namePart><namePart type="family">Vennari</namePart><affiliation>Senior Services of America, Silver Spring, Maryland 20910, USA</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">Rodney L.</namePart><namePart type="family">Danner</namePart><affiliation>Senior Services of America, Silver Spring, Maryland 20910, USA</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">Kent</namePart><namePart type="family">Smith</namePart><affiliation>Senior Services of America, Silver Spring, Maryland 20910, USA</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">Blaze R.</namePart><namePart type="family">Manfredonia</namePart><affiliation>Senior Services of America, Silver Spring, Maryland 20910, USA</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">Dannielle H.</namePart><namePart type="family">Kulaw</namePart><affiliation>Student Services Contractor, Ft. Walton Beach, Florida 32547, USA</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">Michael J.</namePart><namePart type="family">Hemmer</namePart><affiliation>U.S. Environmental Protection Agency, Office of Research and Development, National Health and Environmental Effects Research Laboratory, Gulf Ecology Division, Gulf Breeze, Florida 32561</affiliation><role><roleTerm type="text">author</roleTerm></role></name><typeOfResource>text</typeOfResource><genre type="article" displayLabel="article"></genre><originInfo><publisher>Wiley Periodicals, Inc.</publisher><place><placeTerm type="text">Hoboken</placeTerm></place><dateIssued encoding="w3cdtf">2009-11</dateIssued><dateCaptured encoding="w3cdtf">2008-10-28</dateCaptured><dateValid encoding="w3cdtf">2009-06-10</dateValid><copyrightDate encoding="w3cdtf">2009</copyrightDate></originInfo><language><languageTerm type="code" authority="rfc3066">en</languageTerm><languageTerm type="code" authority="iso639-2b">eng</languageTerm></language><physicalDescription><internetMediaType>text/html</internetMediaType><extent unit="figures">6</extent><extent unit="tables">2</extent><extent unit="references">46</extent></physicalDescription><abstract lang="en">A 280‐d study examined the effects of 17β‐estradiol (E2) on reproduction and development of the sheepshead minnow (Cyprinodon variegatus) exposed from the parental (F0) through three subsequent (F1, F2, and F3) generations and evaluated the need for multigenerational assessments of the risks of endocrine‐disrupting chemicals. This first three‐generation study exposed adult F0 and F1 fish to measured concentrations of 0.01, 0.04, 0.08, 0.2, and 0.3 μg E2/L; the F2 and F3 generations were exposed to 0.2 μg E2/L or less. The cumulative 21‐d production of normal embryos was significantly reduced in the F0 generation at 0.3 μg E2/L and in the F1 and F2 generations at 0.08 μg E2/L or more. The daily reproductive rate was significantly reduced in all three generations at 0.08 μg E2/L or more during spawning days 8 to 14 and 15 to 21. The proportion of infertile eggs from F1 fish was significantly increased above that of the solvent controls at 0.04 and 0.2 μg E2/L and from F2 fish at 0.04 μg E2/L or more. Changes in liver, kidney, and gonadal tissues were seen in the F0 and F1 generations exposed to 0.2 μg E2/L or more. The female gonadosomatic index was significantly decreased at 0.3 μg E2/L in the F0 and F1 generations. Estradiol affected the hepatosomatic index only in female F1 fish, but not in a dose‐dependent manner. All F1 fish in 0.3 μg E2/L appeared to be phenotypically female. Our results indicate that life‐cycle exposure to E2 significantly decreased embryo production by F1 and F2 fish at concentrations lower than those affecting the F0 generation, and they emphasize the importance of evaluating the impact of an estrogenic chemical on reproduction through a minimum of two (F0 and F1) generations.</abstract><note type="content">*This is contribution 1343 from the Gulf Ecology Division.</note><subject lang="en"><genre>keywords</genre><topic>17β‐Estradiol</topic><topic>Sheepshead minnow</topic><topic>Reproduction</topic><topic>Development</topic><topic>Multiple generation</topic></subject><relatedItem type="host"><titleInfo><title>Environmental Toxicology and Chemistry</title><subTitle>An International Journal</subTitle></titleInfo><titleInfo type="abbreviated"><title>Environmental Toxicology and Chemistry</title></titleInfo><genre type="journal">journal</genre><subject><genre>article-category</genre><topic>Environmental Toxicology</topic></subject><identifier type="ISSN">0730-7268</identifier><identifier type="eISSN">1552-8618</identifier><identifier type="DOI">10.1002/(ISSN)1552-8618</identifier><identifier type="PublisherID">ETC</identifier><part><date>2009</date><detail type="volume"><caption>vol.</caption><number>28</number></detail><detail type="issue"><caption>no.</caption><number>11</number></detail><extent unit="pages"><start>2397</start><end>2408</end><total>12</total></extent></part></relatedItem><identifier type="istex">13674B4245E1E37829A90CA740493C31BB56821A</identifier><identifier type="DOI">10.1897/08-542.1</identifier><identifier type="ArticleID">ETC5620281119</identifier><accessCondition type="use and reproduction" contentType="copyright">Copyright © 2009 SETAC</accessCondition><recordInfo><recordContentSource>WILEY</recordContentSource><recordOrigin>Wiley Periodicals, Inc.</recordOrigin></recordInfo></mods></metadata><serie></serie></istex></record>Pour manipuler ce document sous Unix (Dilib)
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