The transcriptomics of sympatric dwarf and normal lake whitefish (Coregonus clupeaformis spp., Salmonidae) divergence as revealed by next‐generation sequencing
Identifieur interne : 001263 ( Istex/Corpus ); précédent : 001262; suivant : 001264The transcriptomics of sympatric dwarf and normal lake whitefish (Coregonus clupeaformis spp., Salmonidae) divergence as revealed by next‐generation sequencing
Auteurs : Julie Jeukens ; Sébastien Renaut ; Jérôme St-Cyr ; Arne W. Nolte ; Louis BernatchezSource :
- Molecular Ecology [ 0962-1083 ] ; 2010-12.
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Abstract
Gene expression divergence is one of the mechanisms thought to be involved in the emergence of incipient species. Next‐generation sequencing has become an extremely valuable tool for the study of this process by allowing whole transcriptome sequencing, or RNA‐Seq. We have conducted a 454 GS‐FLX pyrosequencing experiment to refine our understanding of adaptive divergence between dwarf and normal lake whitefish species (Coregonus clupeaformis spp.). The objectives were to: (i) investigate transcriptomic divergence as measured by liver RNA‐Seq; (ii) test the correlation between divergence in expression and sequence polymorphism; and (iii) investigate the extent of allelic imbalance. We also compared the results of RNA‐seq with those of a previous microarray study performed on the same fish. Following de novo assembly, results showed that normal whitefish overexpressed more contigs associated with protein synthesis while dwarf fish overexpressed more contigs related to energy metabolism, immunity and DNA replication and repair. Moreover, 63 SNPs showed significant allelic imbalance, and this phenomenon prevailed in the recently diverged dwarf whitefish. Results also showed an absence of correlation between gene expression divergence as measured by RNA‐Seq and either polymorphism rate or sequence divergence between normal and dwarf whitefish. This study reiterates an important role for gene expression divergence, and provides evidence for allele‐specific expression divergence as well as evolutionary decoupling of regulatory and coding sequences in the adaptive divergence of normal and dwarf whitefish. It also demonstrates how next‐generation sequencing can lead to a more comprehensive understanding of transcriptomic divergence in a young species pair.
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DOI: 10.1111/j.1365-294X.2010.04934.x
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Nolte</name><affiliation><mods:affiliation>Max Planck Institute for Evolutionary Biology, August‐Thienemann‐Street 2, 24306 Plön, Germany</mods:affiliation></affiliation></author><author><name sortKey="Bernatchez, Louis" sort="Bernatchez, Louis" uniqKey="Bernatchez L" first="Louis" last="Bernatchez">Louis Bernatchez</name><affiliation><mods:affiliation>Institut de Biologie Intégrative et des Systèmes (IBIS), Québec‐Océan, Université Laval, 1030 av. de la médecine, Québec, QC, G1V 0A6, Canada</mods:affiliation></affiliation></author></titleStmt><publicationStmt><idno type="wicri:source">ISTEX</idno><idno type="RBID">ISTEX:ED617AF2E1C5CE6C891D3CCF8FF62732F0A14BCC</idno><date when="2010" year="2010">2010</date><idno type="doi">10.1111/j.1365-294X.2010.04934.x</idno><idno type="url">https://api.istex.fr/document/ED617AF2E1C5CE6C891D3CCF8FF62732F0A14BCC/fulltext/pdf</idno><idno type="wicri:Area/Istex/Corpus">001263</idno><idno type="wicri:explorRef" wicri:stream="Istex" wicri:step="Corpus" wicri:corpus="ISTEX">001263</idno></publicationStmt><sourceDesc><biblStruct><analytic><title level="a" type="main" xml:lang="en">The transcriptomics of sympatric dwarf and normal lake whitefish (Coregonus clupeaformis spp., Salmonidae) divergence as revealed by next‐generation sequencing</title><author><name sortKey="Jeukens, Julie" sort="Jeukens, Julie" uniqKey="Jeukens J" first="Julie" last="Jeukens">Julie Jeukens</name><affiliation><mods:affiliation>Institut de Biologie Intégrative et des Systèmes (IBIS), Québec‐Océan, Université Laval, 1030 av. de la médecine, Québec, QC, G1V 0A6, Canada</mods:affiliation></affiliation></author><author><name sortKey="Renaut, Sebastien" sort="Renaut, Sebastien" uniqKey="Renaut S" first="Sébastien" last="Renaut">Sébastien Renaut</name><affiliation><mods:affiliation>Institut de Biologie Intégrative et des Systèmes (IBIS), Québec‐Océan, Université Laval, 1030 av. de la médecine, Québec, QC, G1V 0A6, Canada</mods:affiliation></affiliation></author><author><name sortKey="St Yr, Jerome" sort="St Yr, Jerome" uniqKey="St Yr J" first="Jérôme" last="St-Cyr">Jérôme St-Cyr</name><affiliation><mods:affiliation>Institut de Biologie Intégrative et des Systèmes (IBIS), Québec‐Océan, Université Laval, 1030 av. de la médecine, Québec, QC, G1V 0A6, Canada</mods:affiliation></affiliation></author><author><name sortKey="Nolte, Arne W" sort="Nolte, Arne W" uniqKey="Nolte A" first="Arne W." last="Nolte">Arne W. 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Next‐generation sequencing has become an extremely valuable tool for the study of this process by allowing whole transcriptome sequencing, or RNA‐Seq. We have conducted a 454 GS‐FLX pyrosequencing experiment to refine our understanding of adaptive divergence between dwarf and normal lake whitefish species (Coregonus clupeaformis spp.). The objectives were to: (i) investigate transcriptomic divergence as measured by liver RNA‐Seq; (ii) test the correlation between divergence in expression and sequence polymorphism; and (iii) investigate the extent of allelic imbalance. We also compared the results of RNA‐seq with those of a previous microarray study performed on the same fish. Following de novo assembly, results showed that normal whitefish overexpressed more contigs associated with protein synthesis while dwarf fish overexpressed more contigs related to energy metabolism, immunity and DNA replication and repair. Moreover, 63 SNPs showed significant allelic imbalance, and this phenomenon prevailed in the recently diverged dwarf whitefish. Results also showed an absence of correlation between gene expression divergence as measured by RNA‐Seq and either polymorphism rate or sequence divergence between normal and dwarf whitefish. This study reiterates an important role for gene expression divergence, and provides evidence for allele‐specific expression divergence as well as evolutionary decoupling of regulatory and coding sequences in the adaptive divergence of normal and dwarf whitefish. 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Next‐generation sequencing has become an extremely valuable tool for the study of this process by allowing whole transcriptome sequencing, or RNA‐Seq. We have conducted a 454 GS‐FLX pyrosequencing experiment to refine our understanding of adaptive divergence between dwarf and normal lake whitefish species (Coregonus clupeaformis spp.). The objectives were to: (i) investigate transcriptomic divergence as measured by liver RNA‐Seq; (ii) test the correlation between divergence in expression and sequence polymorphism; and (iii) investigate the extent of allelic imbalance. We also compared the results of RNA‐seq with those of a previous microarray study performed on the same fish. Following de novo assembly, results showed that normal whitefish overexpressed more contigs associated with protein synthesis while dwarf fish overexpressed more contigs related to energy metabolism, immunity and DNA replication and repair. Moreover, 63 SNPs showed significant allelic imbalance, and this phenomenon prevailed in the recently diverged dwarf whitefish. Results also showed an absence of correlation between gene expression divergence as measured by RNA‐Seq and either polymorphism rate or sequence divergence between normal and dwarf whitefish. This study reiterates an important role for gene expression divergence, and provides evidence for allele‐specific expression divergence as well as evolutionary decoupling of regulatory and coding sequences in the adaptive divergence of normal and dwarf whitefish. It also demonstrates how next‐generation sequencing can lead to a more comprehensive understanding of transcriptomic divergence in a young species pair.</abstract><qualityIndicators><score>7.976</score><pdfVersion>1.3</pdfVersion><pdfPageSize>595.276 x 793.701 pts</pdfPageSize><refBibsNative>true</refBibsNative><abstractCharCount>1776</abstractCharCount><pdfWordCount>9197</pdfWordCount><pdfCharCount>59269</pdfCharCount><pdfPageCount>15</pdfPageCount><abstractWordCount>248</abstractWordCount></qualityIndicators><title>The transcriptomics of sympatric dwarf and normal lake whitefish (Coregonus clupeaformis spp., Salmonidae) divergence as revealed by next‐generation 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Next‐generation sequencing has become an extremely valuable tool for the study of this process by allowing whole transcriptome sequencing, or RNA‐Seq. We have conducted a 454 GS‐FLX pyrosequencing experiment to refine our understanding of adaptive divergence between dwarf and normal lake whitefish species (Coregonus clupeaformis spp.). The objectives were to: (i) investigate transcriptomic divergence as measured by liver RNA‐Seq; (ii) test the correlation between divergence in expression and sequence polymorphism; and (iii) investigate the extent of allelic imbalance. We also compared the results of RNA‐seq with those of a previous microarray study performed on the same fish. Following de novo assembly, results showed that normal whitefish overexpressed more contigs associated with protein synthesis while dwarf fish overexpressed more contigs related to energy metabolism, immunity and DNA replication and repair. Moreover, 63 SNPs showed significant allelic imbalance, and this phenomenon prevailed in the recently diverged dwarf whitefish. Results also showed an absence of correlation between gene expression divergence as measured by RNA‐Seq and either polymorphism rate or sequence divergence between normal and dwarf whitefish. This study reiterates an important role for gene expression divergence, and provides evidence for allele‐specific expression divergence as well as evolutionary decoupling of regulatory and coding sequences in the adaptive divergence of normal and dwarf whitefish. It also demonstrates how next‐generation sequencing can lead to a more comprehensive understanding of transcriptomic divergence in a young species pair.</p></abstract><textClass xml:lang="en"><keywords scheme="keyword"><list><head>keywords</head><item><term>454 sequencing</term></item><item><term>adaptive divergence</term></item><item><term>allelic imbalance</term></item><item><term>Coregonus</term></item><item><term>gene expression</term></item><item><term>RNA‐Seq</term></item><item><term>speciation</term></item><item><term>transcriptome</term></item></list></keywords></textClass></profileDesc><revisionDesc><change when="2010-12">Published</change></revisionDesc></teiHeader></istex:fulltextTEI><json:item><extension>txt</extension><original>false</original><mimetype>text/plain</mimetype><uri>https://api.istex.fr/document/ED617AF2E1C5CE6C891D3CCF8FF62732F0A14BCC/fulltext/txt</uri></json:item></fulltext><metadata><istex:metadataXml wicri:clean="Wiley, elements deleted: body"><istex:xmlDeclaration>version="1.0" encoding="UTF-8" standalone="yes"</istex:xmlDeclaration><istex:document><component version="2.0" type="serialArticle" xml:lang="en"><header><publicationMeta level="product"><publisherInfo><publisherName>Blackwell Publishing Ltd</publisherName><publisherLoc>Oxford, UK</publisherLoc></publisherInfo><doi origin="wiley" registered="yes">10.1111/(ISSN)1365-294X</doi><issn type="print">0962-1083</issn><issn type="electronic">1365-294X</issn><idGroup><id type="product" value="MEC"></id><id type="publisherDivision" value="ST"></id></idGroup><titleGroup><title type="main" sort="MOLECULAR ECOLOGY">Molecular Ecology</title></titleGroup></publicationMeta><publicationMeta level="part" position="12124"><doi origin="wiley">10.1111/mec.2010.19.issue-24</doi><numberingGroup><numbering type="journalVolume" number="19">19</numbering><numbering type="journalIssue" number="24">24</numbering></numberingGroup><coverDate startDate="2010-12">December 2010</coverDate></publicationMeta><publicationMeta level="unit" type="article" position="8" status="forIssue"><doi origin="wiley">10.1111/j.1365-294X.2010.04934.x</doi><idGroup><id type="unit" value="MEC4934"></id></idGroup><countGroup><count type="pageTotal" number="15"></count></countGroup><titleGroup><title type="tocHeading1">ORIGINAL ARTICLES</title><title type="tocHeading2">Ecological Genomics</title></titleGroup><copyright>© 2010 Blackwell Publishing Ltd</copyright><eventGroup><event type="xmlConverted" agent="Converter:BPG_TO_WML3G version:2.4 mode:FullText source:FullText result:FullText" date="2010-12-14"></event><event type="publishedOnlineEarlyUnpaginated" date="2010-11-19"></event><event type="publishedOnlineFinalForm" date="2010-12-06"></event><event type="firstOnline" date="2010-11-19"></event><event type="xmlConverted" agent="Converter:WILEY_ML3G_TO_WILEY_ML3GV2 version:4.0.1" date="2014-03-20"></event><event type="xmlConverted" agent="Converter:WML3G_To_WML3G version:4.1.7 mode:FullText,remove_FC" date="2014-10-31"></event></eventGroup><numberingGroup><numbering type="pageFirst" number="5389">5389</numbering><numbering type="pageLast" number="5403">5403</numbering></numberingGroup><correspondenceTo>Julie Jeukens, Fax: 1 418 656 7176; E‐mail: <email>julie.jeukens.1@ulaval.ca</email></correspondenceTo><linkGroup><link type="toTypesetVersion" href="file:MEC.MEC4934.pdf"></link></linkGroup></publicationMeta><contentMeta><unparsedEditorialHistory>Received 7 June 2010; revision received 18 August 2010; accepted 29 August 2010</unparsedEditorialHistory><countGroup><count type="figureTotal" number="5"></count><count type="tableTotal" number="4"></count></countGroup><titleGroup><title type="main">The transcriptomics of sympatric dwarf and normal lake whitefish (<i>Coregonus clupeaformis</i> spp., Salmonidae) divergence as revealed by next‐generation sequencing</title><title type="shortAuthors">J. JEUKENS <i>ET AL.</i></title><title type="short">TRANSCRIPTOMIC DIVERGENCE IN LAKE WHITEFISH</title></titleGroup><creators><creator creatorRole="author" xml:id="cr1" affiliationRef="#a1"><personName><givenNames>JULIE</givenNames><familyName>JEUKENS</familyName></personName></creator><creator creatorRole="author" xml:id="cr2" affiliationRef="#a1"><personName><givenNames>SÉBASTIEN</givenNames><familyName>RENAUT</familyName></personName></creator><creator creatorRole="author" xml:id="cr3" affiliationRef="#a1"><personName><givenNames>JÉRÔME</givenNames><familyName>ST‐CYR</familyName></personName></creator><creator creatorRole="author" xml:id="cr4" affiliationRef="#a2"><personName><givenNames>ARNE W.</givenNames><familyName>NOLTE</familyName></personName></creator><creator creatorRole="author" xml:id="cr5" affiliationRef="#a1"><personName><givenNames>LOUIS</givenNames><familyName>BERNATCHEZ</familyName></personName></creator></creators><affiliationGroup><affiliation xml:id="a1" countryCode="CA"><unparsedAffiliation>Institut de Biologie Intégrative et des Systèmes (IBIS), Québec‐Océan, Université Laval, 1030 av. de la médecine, Québec, QC, G1V 0A6, Canada</unparsedAffiliation></affiliation><affiliation xml:id="a2" countryCode="DE"><unparsedAffiliation>Max Planck Institute for Evolutionary Biology, August‐Thienemann‐Street 2, 24306 Plön, Germany</unparsedAffiliation></affiliation></affiliationGroup><keywordGroup xml:lang="en"><keyword xml:id="k1">454 sequencing</keyword><keyword xml:id="k2">adaptive divergence</keyword><keyword xml:id="k3">allelic imbalance</keyword><keyword xml:id="k4">Coregonus</keyword><keyword xml:id="k5">gene expression</keyword><keyword xml:id="k6">RNA‐Seq</keyword><keyword xml:id="k7">speciation</keyword><keyword xml:id="k8">transcriptome</keyword></keywordGroup><supportingInformation><p><b>Table S1</b> Functional gene categories</p><p><b>Table S2</b> <i>De novo</i> contigs from RNA‐Seq of the whitefish liver transcriptome</p><p><b>Table S3</b> Representation of functional gene categories in the whitefish liver transcriptome</p><p><b>Table S4</b> Candidate genes for allelic imbalance (AI)</p><p><b>Fig. S1</b> Scatter plot of gene expression divergence measured by RNA‐Seq and microarray.</p><supportingInfoItem><mediaResource alt="supporting info item" href="urn-x:wiley:09621083:media:mec4934:MEC_4934_sm_ts1-ts3-ts4-fs1"></mediaResource><caption>Supporting info item</caption></supportingInfoItem><supportingInfoItem><mediaResource alt="supporting info item" href="urn-x:wiley:09621083:media:mec4934:MEC_4934_sm_tS2"></mediaResource><caption>Supporting info item</caption></supportingInfoItem></supportingInformation><abstractGroup><abstract type="main" xml:lang="en"><title type="main">Abstract</title><p>Gene expression divergence is one of the mechanisms thought to be involved in the emergence of incipient species. Next‐generation sequencing has become an extremely valuable tool for the study of this process by allowing whole transcriptome sequencing, or RNA‐Seq. We have conducted a 454 GS‐FLX pyrosequencing experiment to refine our understanding of adaptive divergence between dwarf and normal lake whitefish species (<i>Coregonus clupeaformis</i> spp.). The objectives were to: (i) investigate transcriptomic divergence as measured by liver RNA‐Seq; (ii) test the correlation between divergence in expression and sequence polymorphism; and (iii) investigate the extent of allelic imbalance. We also compared the results of RNA‐seq with those of a previous microarray study performed on the same fish. Following <i>de novo</i> assembly, results showed that normal whitefish overexpressed more contigs associated with protein synthesis while dwarf fish overexpressed more contigs related to energy metabolism, immunity and DNA replication and repair. Moreover, 63 SNPs showed significant allelic imbalance, and this phenomenon prevailed in the recently diverged dwarf whitefish. Results also showed an absence of correlation between gene expression divergence as measured by RNA‐Seq and either polymorphism rate or sequence divergence between normal and dwarf whitefish. This study reiterates an important role for gene expression divergence, and provides evidence for allele‐specific expression divergence as well as evolutionary decoupling of regulatory and coding sequences in the adaptive divergence of normal and dwarf whitefish. It also demonstrates how next‐generation sequencing can lead to a more comprehensive understanding of transcriptomic divergence in a young species pair.</p></abstract></abstractGroup></contentMeta></header></component></istex:document></istex:metadataXml><mods version="3.6"><titleInfo lang="en"><title>The transcriptomics of sympatric dwarf and normal lake whitefish (Coregonus clupeaformis spp., Salmonidae) divergence as revealed by next‐generation sequencing</title></titleInfo><titleInfo type="abbreviated" lang="en"><title>TRANSCRIPTOMIC DIVERGENCE IN LAKE WHITEFISH</title></titleInfo><titleInfo type="alternative" contentType="CDATA" lang="en"><title>The transcriptomics of sympatric dwarf and normal lake whitefish (Coregonus clupeaformis spp., Salmonidae) divergence as revealed by next‐generation sequencing</title></titleInfo><name type="personal"><namePart type="given">JULIE</namePart><namePart type="family">JEUKENS</namePart><affiliation>Institut de Biologie Intégrative et des Systèmes (IBIS), Québec‐Océan, Université Laval, 1030 av. de la médecine, Québec, QC, G1V 0A6, Canada</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">SÉBASTIEN</namePart><namePart type="family">RENAUT</namePart><affiliation>Institut de Biologie Intégrative et des Systèmes (IBIS), Québec‐Océan, Université Laval, 1030 av. de la médecine, Québec, QC, G1V 0A6, Canada</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">JÉRÔME</namePart><namePart type="family">ST‐CYR</namePart><affiliation>Institut de Biologie Intégrative et des Systèmes (IBIS), Québec‐Océan, Université Laval, 1030 av. de la médecine, Québec, QC, G1V 0A6, Canada</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">ARNE W.</namePart><namePart type="family">NOLTE</namePart><affiliation>Max Planck Institute for Evolutionary Biology, August‐Thienemann‐Street 2, 24306 Plön, Germany</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">LOUIS</namePart><namePart type="family">BERNATCHEZ</namePart><affiliation>Institut de Biologie Intégrative et des Systèmes (IBIS), Québec‐Océan, Université Laval, 1030 av. de la médecine, Québec, QC, G1V 0A6, Canada</affiliation><role><roleTerm type="text">author</roleTerm></role></name><typeOfResource>text</typeOfResource><genre type="article" displayLabel="article"></genre><originInfo><publisher>Blackwell Publishing Ltd</publisher><place><placeTerm type="text">Oxford, UK</placeTerm></place><dateIssued encoding="w3cdtf">2010-12</dateIssued><edition>Received 7 June 2010; revision received 18 August 2010; accepted 29 August 2010</edition><copyrightDate encoding="w3cdtf">2010</copyrightDate></originInfo><language><languageTerm type="code" authority="rfc3066">en</languageTerm><languageTerm type="code" authority="iso639-2b">eng</languageTerm></language><physicalDescription><internetMediaType>text/html</internetMediaType><extent unit="figures">5</extent><extent unit="tables">4</extent></physicalDescription><abstract lang="en">Gene expression divergence is one of the mechanisms thought to be involved in the emergence of incipient species. Next‐generation sequencing has become an extremely valuable tool for the study of this process by allowing whole transcriptome sequencing, or RNA‐Seq. We have conducted a 454 GS‐FLX pyrosequencing experiment to refine our understanding of adaptive divergence between dwarf and normal lake whitefish species (Coregonus clupeaformis spp.). The objectives were to: (i) investigate transcriptomic divergence as measured by liver RNA‐Seq; (ii) test the correlation between divergence in expression and sequence polymorphism; and (iii) investigate the extent of allelic imbalance. We also compared the results of RNA‐seq with those of a previous microarray study performed on the same fish. Following de novo assembly, results showed that normal whitefish overexpressed more contigs associated with protein synthesis while dwarf fish overexpressed more contigs related to energy metabolism, immunity and DNA replication and repair. Moreover, 63 SNPs showed significant allelic imbalance, and this phenomenon prevailed in the recently diverged dwarf whitefish. Results also showed an absence of correlation between gene expression divergence as measured by RNA‐Seq and either polymorphism rate or sequence divergence between normal and dwarf whitefish. This study reiterates an important role for gene expression divergence, and provides evidence for allele‐specific expression divergence as well as evolutionary decoupling of regulatory and coding sequences in the adaptive divergence of normal and dwarf whitefish. It also demonstrates how next‐generation sequencing can lead to a more comprehensive understanding of transcriptomic divergence in a young species pair.</abstract><subject lang="en"><genre>keywords</genre><topic>454 sequencing</topic><topic>adaptive divergence</topic><topic>allelic imbalance</topic><topic>Coregonus</topic><topic>gene expression</topic><topic>RNA‐Seq</topic><topic>speciation</topic><topic>transcriptome</topic></subject><relatedItem type="host"><titleInfo><title>Molecular Ecology</title></titleInfo><genre type="journal">journal</genre><note type="content"> Table S1 Functional gene categories Table S2 De novo contigs from RNA‐Seq of the whitefish liver transcriptome Table S3 Representation of functional gene categories in the whitefish liver transcriptome Table S4 Candidate genes for allelic imbalance (AI) Fig. S1 Scatter plot of gene expression divergence measured by RNA‐Seq and microarray. Table S1 Functional gene categories Table S2 De novo contigs from RNA‐Seq of the whitefish liver transcriptome Table S3 Representation of functional gene categories in the whitefish liver transcriptome Table S4 Candidate genes for allelic imbalance (AI) Fig. S1 Scatter plot of gene expression divergence measured by RNA‐Seq and microarray. Table S1 Functional gene categories Table S2 De novo contigs from RNA‐Seq of the whitefish liver transcriptome Table S3 Representation of functional gene categories in the whitefish liver transcriptome Table S4 Candidate genes for allelic imbalance (AI) Fig. S1 Scatter plot of gene expression divergence measured by RNA‐Seq and microarray. Table S1 Functional gene categories Table S2 De novo contigs from RNA‐Seq of the whitefish liver transcriptome Table S3 Representation of functional gene categories in the whitefish liver transcriptome Table S4 Candidate genes for allelic imbalance (AI) Fig. S1 Scatter plot of gene expression divergence measured by RNA‐Seq and microarray. Table S1 Functional gene categories Table S2 De novo contigs from RNA‐Seq of the whitefish liver transcriptome Table S3 Representation of functional gene categories in the whitefish liver transcriptome Table S4 Candidate genes for allelic imbalance (AI) Fig. S1 Scatter plot of gene expression divergence measured by RNA‐Seq and microarray.Supporting Info Item: Supporting info item - Supporting info item - </note><identifier type="ISSN">0962-1083</identifier><identifier type="eISSN">1365-294X</identifier><identifier type="DOI">10.1111/(ISSN)1365-294X</identifier><identifier type="PublisherID">MEC</identifier><part><date>2010</date><detail type="volume"><caption>vol.</caption><number>19</number></detail><detail type="issue"><caption>no.</caption><number>24</number></detail><extent unit="pages"><start>5389</start><end>5403</end><total>15</total></extent></part></relatedItem><identifier type="istex">ED617AF2E1C5CE6C891D3CCF8FF62732F0A14BCC</identifier><identifier type="DOI">10.1111/j.1365-294X.2010.04934.x</identifier><identifier type="ArticleID">MEC4934</identifier><accessCondition type="use and reproduction" contentType="copyright">© 2010 Blackwell Publishing Ltd</accessCondition><recordInfo><recordContentSource>WILEY</recordContentSource><recordOrigin>Blackwell Publishing Ltd</recordOrigin></recordInfo></mods></metadata><serie></serie></istex></record>Pour manipuler ce document sous Unix (Dilib)
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