Tilapia (Oreochromis niloticus) vitellogenins: development of homologous and heterologous ELISAs and analysis of vitellogenin pathway through the ovarian follicle
Identifieur interne : 001059 ( Istex/Corpus ); précédent : 001058; suivant : 001060Tilapia (Oreochromis niloticus) vitellogenins: development of homologous and heterologous ELISAs and analysis of vitellogenin pathway through the ovarian follicle
Auteurs : Pap Ndiaye ; Jean Forgue ; Valérie Lamothe ; Chantal Cauty ; Philippe Tacon ; Pierrette Lafon ; Blandine Davail ; Alexis Fostier ; Françoise Le Menn ; Jesús Nú EzSource :
- Journal of Experimental Zoology Part A: Comparative Experimental Biology [ 1548-8969 ] ; 2006-07-01.
Abstract
Vitellogenin (VTG) of Oreochromis niloticus was again purified, due to the conflicting results found in the literature. Three purification processes have been used: electrophoresis and electro‐elution, double chromatography (gel filtration and ion‐exchange chromatography) and single ion‐exchange chromatography. Using SDS‐PAGE we confirmed in all cases the presence of two polypeptidic forms of plasma VTG of 130 kDa (VTG1) and 170 kDa (VTG2). We raised polyclonal antibodies against each VTG form and we demonstrated the complete cross‐reactivity of each antibody with both forms of VTG by Enzyme Immuno‐Assay (EIA) and Western blots. The homologous ELISAs developed exhibited a detection limit of 6 ng.ml−1, equivalent to 60 ng.ml−1 of plasma VTG and allowed us to quantify the total plasma VTG of O. niloticus with high specificity and sensitivity. Using photonic and electron immunomicroscopy, we followed the pathway of VTG into the ovarian follicle (OF) demonstrating that VTG enters the oocyte at stage 3 of OF development, at the same time as cortical alveoli and lipid globules appear. Heterologous ELISAs performed on other cichlid species allowed us to quantify plasma VTG in Oreochromis aureus and Sarotherodon melanotheron and to detect it in Hemichromis fasciatus, Hemichromis bimaculatus and Tilapia zillii, constituting a reliable tool for monitoring the presence of xeno‐estrogens in the environment of these fish species. J. Exp. Zool. 305A, 2006. © 2006 Wiley‐Liss, Inc.
Url:
DOI: 10.1002/jez.a.290
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ISTEX:93A5A5BE4EA64A2C273135E47EB93F1F05942A13Le document en format XML
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Three purification processes have been used: electrophoresis and electro‐elution, double chromatography (gel filtration and ion‐exchange chromatography) and single ion‐exchange chromatography. Using SDS‐PAGE we confirmed in all cases the presence of two polypeptidic forms of plasma VTG of 130 kDa (VTG1) and 170 kDa (VTG2). We raised polyclonal antibodies against each VTG form and we demonstrated the complete cross‐reactivity of each antibody with both forms of VTG by Enzyme Immuno‐Assay (EIA) and Western blots. The homologous ELISAs developed exhibited a detection limit of 6 ng.ml−1, equivalent to 60 ng.ml−1 of plasma VTG and allowed us to quantify the total plasma VTG of O. niloticus with high specificity and sensitivity. Using photonic and electron immunomicroscopy, we followed the pathway of VTG into the ovarian follicle (OF) demonstrating that VTG enters the oocyte at stage 3 of OF development, at the same time as cortical alveoli and lipid globules appear. Heterologous ELISAs performed on other cichlid species allowed us to quantify plasma VTG in Oreochromis aureus and Sarotherodon melanotheron and to detect it in Hemichromis fasciatus, Hemichromis bimaculatus and Tilapia zillii, constituting a reliable tool for monitoring the presence of xeno‐estrogens in the environment of these fish species. J. Exp. 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We raised polyclonal antibodies against each VTG form and we demonstrated the complete cross‐reactivity of each antibody with both forms of VTG by Enzyme Immuno‐Assay (EIA) and Western blots. The homologous ELISAs developed exhibited a detection limit of 6 ng.ml−1, equivalent to 60 ng.ml−1 of plasma VTG and allowed us to quantify the total plasma VTG of O. niloticus with high specificity and sensitivity. Using photonic and electron immunomicroscopy, we followed the pathway of VTG into the ovarian follicle (OF) demonstrating that VTG enters the oocyte at stage 3 of OF development, at the same time as cortical alveoli and lipid globules appear. Heterologous ELISAs performed on other cichlid species allowed us to quantify plasma VTG in Oreochromis aureus and Sarotherodon melanotheron and to detect it in Hemichromis fasciatus, Hemichromis bimaculatus and Tilapia zillii, constituting a reliable tool for monitoring the presence of xeno‐estrogens in the environment of these fish species. J. 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type="first">Pap</forename><surname>Ndiaye</surname></persName><affiliation>IFAN, Université Cheikh Anta Diop, Dakar, Sénégal</affiliation></author><author xml:id="author-2"><persName><forename type="first">Jean</forename><surname>Forgue</surname></persName><affiliation>Laboratoire de Génomique et de Physiologie des Poissons, Université de Bordeaux I, 33405 Talence, France</affiliation></author><author xml:id="author-3"><persName><forename type="first">Valérie</forename><surname>Lamothe</surname></persName><affiliation>Unité Micronutriments Reproduction Santé, ENITAB, 1 cours du général de Gaulle, 33175 Gradignan cedex, France</affiliation></author><author xml:id="author-4"><persName><forename type="first">Chantal</forename><surname>Cauty</surname></persName><affiliation>INRA SCRIBE, campus de Beaulieu, 35042 Rennes cedex, France</affiliation></author><author xml:id="author-5"><persName><forename type="first">Philippe</forename><surname>Tacon</surname></persName><affiliation>INRA SCRIBE, campus de Beaulieu, 35042 Rennes cedex, France</affiliation></author><author xml:id="author-6"><persName><forename type="first">Pierrette</forename><surname>Lafon</surname></persName><affiliation>Laboratoire des Régulations Neuroendocriniennes, EA 2972, Université de Bordeaux I, 33405 Talence cedex, France</affiliation></author><author xml:id="author-7"><persName><forename type="first">Blandine</forename><surname>Davail</surname></persName><affiliation>Laboratoire de Génomique et de Physiologie des Poissons, Université de Bordeaux I, 33405 Talence, France</affiliation></author><author xml:id="author-8"><persName><forename type="first">Alexis</forename><surname>Fostier</surname></persName><affiliation>INRA SCRIBE, campus de Beaulieu, 35042 Rennes cedex, France</affiliation></author><author xml:id="author-9"><persName><forename type="first">Françoise LE</forename><surname>Menn</surname></persName><affiliation>Laboratoire de Génomique et de Physiologie des Poissons, Université de 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Zool.</title><idno type="pISSN">1548-8969</idno><idno type="eISSN">1552-499X</idno><idno type="DOI">10.1002/(ISSN)1552-499X</idno><imprint><publisher>Wiley Subscription Services, Inc., A Wiley Company</publisher><pubPlace>Hoboken</pubPlace><date type="published" when="2006-07-01"></date><biblScope unit="volume">305A</biblScope><biblScope unit="issue">7</biblScope><biblScope unit="page" from="576">576</biblScope><biblScope unit="page" to="593">593</biblScope></imprint></monogr><idno type="istex">93A5A5BE4EA64A2C273135E47EB93F1F05942A13</idno><idno type="DOI">10.1002/jez.a.290</idno><idno type="ArticleID">JEZ290</idno></biblStruct></sourceDesc></fileDesc><profileDesc><creation><date>2006</date></creation><langUsage><language ident="en">en</language></langUsage><abstract xml:lang="en"><p>Vitellogenin (VTG) of Oreochromis niloticus was again purified, due to the conflicting results found in the literature. Three purification processes have been used: electrophoresis and electro‐elution, double chromatography (gel filtration and ion‐exchange chromatography) and single ion‐exchange chromatography. Using SDS‐PAGE we confirmed in all cases the presence of two polypeptidic forms of plasma VTG of 130 kDa (VTG1) and 170 kDa (VTG2). We raised polyclonal antibodies against each VTG form and we demonstrated the complete cross‐reactivity of each antibody with both forms of VTG by Enzyme Immuno‐Assay (EIA) and Western blots. The homologous ELISAs developed exhibited a detection limit of 6 ng.ml−1, equivalent to 60 ng.ml−1 of plasma VTG and allowed us to quantify the total plasma VTG of O. niloticus with high specificity and sensitivity. Using photonic and electron immunomicroscopy, we followed the pathway of VTG into the ovarian follicle (OF) demonstrating that VTG enters the oocyte at stage 3 of OF development, at the same time as cortical alveoli and lipid globules appear. Heterologous ELISAs performed on other cichlid species allowed us to quantify plasma VTG in Oreochromis aureus and Sarotherodon melanotheron and to detect it in Hemichromis fasciatus, Hemichromis bimaculatus and Tilapia zillii, constituting a reliable tool for monitoring the presence of xeno‐estrogens in the environment of these fish species. J. Exp. 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Zool.</title></titleGroup><selfCitationGroup><citation type="ancestor" xml:id="cit1"><journalTitle>Journal of Experimental Zoology</journalTitle><accessionId ref="info:x-wiley/issn/0022104X">0022-104X</accessionId><accessionId ref="info:x-wiley/issn/1097010X">1097-010X</accessionId><pubYear year="2004">2004</pubYear></citation></selfCitationGroup></publicationMeta><publicationMeta level="part" position="70"><doi origin="wiley" registered="yes">10.1002/jez.a.v305a:7</doi><numberingGroup><numbering type="journalVolume" number="305">305A</numbering><numbering type="journalIssue">7</numbering></numberingGroup><coverDate startDate="2006-07-01">1 July 2006</coverDate></publicationMeta><publicationMeta level="unit" type="article" position="6" status="forIssue"><doi origin="wiley" registered="yes">10.1002/jez.a.290</doi><idGroup><id type="unit" value="JEZ290"></id></idGroup><countGroup><count type="pageTotal" number="18"></count></countGroup><titleGroup><title type="articleCategory">Reproductive Biology</title><title type="tocHeading1">Reproductive Biology</title></titleGroup><copyright ownership="publisher">Copyright © 2006 Wiley‐Liss, Inc., A Wiley Company</copyright><eventGroup><event type="manuscriptReceived" date="2005-08-15"></event><event type="manuscriptAccepted" date="2006-01-24"></event><event type="publishedOnlineEarlyUnpaginated" date="2006-04-13"></event><event type="firstOnline" date="2006-04-13"></event><event type="publishedOnlineFinalForm" date="2006-06-05"></event><event type="xmlConverted" agent="Converter:JWSART34_TO_WML3G version:2.3.6 mode:FullText source:HeaderRef result:HeaderRef" date="2010-05-07"></event><event type="xmlConverted" agent="Converter:WILEY_ML3G_TO_WILEY_ML3GV2 version:4.0.1" date="2014-03-19"></event><event type="xmlConverted" agent="Converter:WML3G_To_WML3G version:4.1.7 mode:FullText,remove_FC" date="2014-10-30"></event></eventGroup><numberingGroup><numbering type="pageFirst">576</numbering><numbering type="pageLast">593</numbering></numberingGroup><correspondenceTo>IRD, Groupe Aquaculture Continentale Méditerranéenne et Tropicale (GAMET), UR 175, CAVIAR, Av. Agropolis, 34033 Montpellier cedex, France===</correspondenceTo><linkGroup><link type="toTypesetVersion" href="file:JEZ.JEZ290.pdf"></link></linkGroup></publicationMeta><contentMeta><countGroup><count type="figureTotal" number="14"></count><count type="tableTotal" number="1"></count><count type="referenceTotal" number="92"></count></countGroup><titleGroup><title type="main" xml:lang="en">Tilapia (<i>Oreochromis niloticus</i>) vitellogenins: development of homologous and heterologous ELISAs and analysis of vitellogenin pathway through the ovarian follicle</title><title type="short" xml:lang="en">ISOLATION AND ELISA OF NILE TILAPIA VITELLOGENINS</title></titleGroup><creators><creator xml:id="au1" creatorRole="author" affiliationRef="#af1"><personName><givenNames>Pap</givenNames><familyName>Ndiaye</familyName></personName></creator><creator xml:id="au2" creatorRole="author" affiliationRef="#af2"><personName><givenNames>Jean</givenNames><familyName>Forgue</familyName></personName></creator><creator xml:id="au3" creatorRole="author" affiliationRef="#af3"><personName><givenNames>Valérie</givenNames><familyName>Lamothe</familyName></personName></creator><creator xml:id="au4" creatorRole="author" affiliationRef="#af4"><personName><givenNames>Chantal</givenNames><familyName>Cauty</familyName></personName></creator><creator xml:id="au5" creatorRole="author" affiliationRef="#af4"><personName><givenNames>Philippe</givenNames><familyName>Tacon</familyName></personName></creator><creator xml:id="au6" creatorRole="author" affiliationRef="#af5"><personName><givenNames>Pierrette</givenNames><familyName>Lafon</familyName></personName></creator><creator xml:id="au7" creatorRole="author" affiliationRef="#af2"><personName><givenNames>Blandine</givenNames><familyName>Davail</familyName></personName></creator><creator xml:id="au8" creatorRole="author" affiliationRef="#af4"><personName><givenNames>Alexis</givenNames><familyName>Fostier</familyName></personName></creator><creator xml:id="au9" creatorRole="author" affiliationRef="#af2"><personName><givenNames>Françoise LE</givenNames><familyName>Menn</familyName></personName></creator><creator xml:id="au10" creatorRole="author" affiliationRef="#af6" corresponding="yes"><personName><givenNames>Jesús</givenNames><familyName>Núñez</familyName></personName><contactDetails><email>nunez@ird.fr</email></contactDetails></creator></creators><affiliationGroup><affiliation xml:id="af1" countryCode="US" type="organization"><unparsedAffiliation>IFAN, Université Cheikh Anta Diop, Dakar, Sénégal</unparsedAffiliation></affiliation><affiliation xml:id="af2" countryCode="FR" type="organization"><unparsedAffiliation>Laboratoire de Génomique et de Physiologie des Poissons, Université de Bordeaux I, 33405 Talence, France</unparsedAffiliation></affiliation><affiliation xml:id="af3" countryCode="FR" type="organization"><unparsedAffiliation>Unité Micronutriments Reproduction Santé, ENITAB, 1 cours du général de Gaulle, 33175 Gradignan cedex, France</unparsedAffiliation></affiliation><affiliation xml:id="af4" countryCode="FR" type="organization"><unparsedAffiliation>INRA SCRIBE, campus de Beaulieu, 35042 Rennes cedex, France</unparsedAffiliation></affiliation><affiliation xml:id="af5" countryCode="FR" type="organization"><unparsedAffiliation>Laboratoire des Régulations Neuroendocriniennes, EA 2972, Université de Bordeaux I, 33405 Talence cedex, France</unparsedAffiliation></affiliation><affiliation xml:id="af6" countryCode="FR" type="organization"><unparsedAffiliation>IRD, Groupe Aquaculture Continentale Méditerranéenne et Tropicale (GAMET), UR 175 CAVIAR, Av. Agropolis, 34033 Montpellier cedex, France</unparsedAffiliation></affiliation></affiliationGroup><abstractGroup><abstract type="main" xml:lang="en"><title type="main">Abstract</title><p>Vitellogenin (VTG) of <i>Oreochromis niloticus</i> was again purified, due to the conflicting results found in the literature. Three purification processes have been used: electrophoresis and electro‐elution, double chromatography (gel filtration and ion‐exchange chromatography) and single ion‐exchange chromatography. Using SDS‐PAGE we confirmed in all cases the presence of two polypeptidic forms of plasma VTG of 130 kDa (VTG<sub>1</sub>) and 170 kDa (VTG<sub>2</sub>). We raised polyclonal antibodies against each VTG form and we demonstrated the complete cross‐reactivity of each antibody with both forms of VTG by Enzyme Immuno‐Assay (EIA) and Western blots. The homologous ELISAs developed exhibited a detection limit of 6 ng.ml<sup>−1</sup>, equivalent to 60 ng.ml<sup>−1</sup> of plasma VTG and allowed us to quantify the total plasma VTG of <i>O. niloticus</i> with high specificity and sensitivity. Using photonic and electron immunomicroscopy, we followed the pathway of VTG into the ovarian follicle (OF) demonstrating that VTG enters the oocyte at stage 3 of OF development, at the same time as cortical alveoli and lipid globules appear. Heterologous ELISAs performed on other cichlid species allowed us to quantify plasma VTG in <i>Oreochromis aureus</i> and <i>Sarotherodon melanotheron</i> and to detect it in <i>Hemichromis fasciatus</i>, <i>Hemichromis bimaculatus</i> and <i>Tilapia zillii</i>, constituting a reliable tool for monitoring the presence of xeno‐estrogens in the environment of these fish species. <i>J. Exp. Zool. 305A, 2006.</i> © 2006 Wiley‐Liss, Inc.</p></abstract></abstractGroup></contentMeta></header></component></istex:document></istex:metadataXml><mods version="3.6"><titleInfo lang="en"><title>Tilapia (Oreochromis niloticus) vitellogenins: development of homologous and heterologous ELISAs and analysis of vitellogenin pathway through the ovarian follicle</title></titleInfo><titleInfo type="abbreviated" lang="en"><title>ISOLATION AND ELISA OF NILE TILAPIA VITELLOGENINS</title></titleInfo><titleInfo type="alternative" contentType="CDATA" lang="en"><title>Tilapia (Oreochromis niloticus) vitellogenins: development of homologous and heterologous ELISAs and analysis of vitellogenin pathway through the ovarian follicle</title></titleInfo><name type="personal"><namePart type="given">Pap</namePart><namePart type="family">Ndiaye</namePart><affiliation>IFAN, Université Cheikh Anta Diop, Dakar, Sénégal</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">Jean</namePart><namePart type="family">Forgue</namePart><affiliation>Laboratoire de Génomique et de Physiologie des Poissons, Université de Bordeaux I, 33405 Talence, France</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">Valérie</namePart><namePart type="family">Lamothe</namePart><affiliation>Unité Micronutriments Reproduction Santé, ENITAB, 1 cours du général de Gaulle, 33175 Gradignan cedex, France</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">Chantal</namePart><namePart type="family">Cauty</namePart><affiliation>INRA SCRIBE, campus de Beaulieu, 35042 Rennes cedex, France</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">Philippe</namePart><namePart type="family">Tacon</namePart><affiliation>INRA SCRIBE, campus de Beaulieu, 35042 Rennes cedex, France</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">Pierrette</namePart><namePart type="family">Lafon</namePart><affiliation>Laboratoire des Régulations Neuroendocriniennes, EA 2972, Université de Bordeaux I, 33405 Talence cedex, France</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">Blandine</namePart><namePart type="family">Davail</namePart><affiliation>Laboratoire de Génomique et de Physiologie des Poissons, Université de Bordeaux I, 33405 Talence, France</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">Alexis</namePart><namePart type="family">Fostier</namePart><affiliation>INRA SCRIBE, campus de Beaulieu, 35042 Rennes cedex, France</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">Françoise LE</namePart><namePart type="family">Menn</namePart><affiliation>Laboratoire de Génomique et de Physiologie des Poissons, Université de Bordeaux I, 33405 Talence, France</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">Jesús</namePart><namePart type="family">Núñez</namePart><affiliation>IRD, Groupe Aquaculture Continentale Méditerranéenne et Tropicale (GAMET), UR 175 CAVIAR, Av. Agropolis, 34033 Montpellier cedex, France</affiliation><affiliation>IRD, Groupe Aquaculture Continentale Méditerranéenne et Tropicale (GAMET), UR 175, CAVIAR, Av. Agropolis, 34033 Montpellier cedex, France===</affiliation><role><roleTerm type="text">author</roleTerm></role></name><typeOfResource>text</typeOfResource><genre type="article" displayLabel="article"></genre><originInfo><publisher>Wiley Subscription Services, Inc., A Wiley Company</publisher><place><placeTerm type="text">Hoboken</placeTerm></place><dateIssued encoding="w3cdtf">2006-07-01</dateIssued><dateCaptured encoding="w3cdtf">2005-08-15</dateCaptured><dateValid encoding="w3cdtf">2006-01-24</dateValid><copyrightDate encoding="w3cdtf">2006</copyrightDate></originInfo><language><languageTerm type="code" authority="rfc3066">en</languageTerm><languageTerm type="code" authority="iso639-2b">eng</languageTerm></language><physicalDescription><internetMediaType>text/html</internetMediaType><extent unit="figures">14</extent><extent unit="tables">1</extent><extent unit="references">92</extent></physicalDescription><abstract lang="en">Vitellogenin (VTG) of Oreochromis niloticus was again purified, due to the conflicting results found in the literature. Three purification processes have been used: electrophoresis and electro‐elution, double chromatography (gel filtration and ion‐exchange chromatography) and single ion‐exchange chromatography. Using SDS‐PAGE we confirmed in all cases the presence of two polypeptidic forms of plasma VTG of 130 kDa (VTG1) and 170 kDa (VTG2). We raised polyclonal antibodies against each VTG form and we demonstrated the complete cross‐reactivity of each antibody with both forms of VTG by Enzyme Immuno‐Assay (EIA) and Western blots. The homologous ELISAs developed exhibited a detection limit of 6 ng.ml−1, equivalent to 60 ng.ml−1 of plasma VTG and allowed us to quantify the total plasma VTG of O. niloticus with high specificity and sensitivity. Using photonic and electron immunomicroscopy, we followed the pathway of VTG into the ovarian follicle (OF) demonstrating that VTG enters the oocyte at stage 3 of OF development, at the same time as cortical alveoli and lipid globules appear. Heterologous ELISAs performed on other cichlid species allowed us to quantify plasma VTG in Oreochromis aureus and Sarotherodon melanotheron and to detect it in Hemichromis fasciatus, Hemichromis bimaculatus and Tilapia zillii, constituting a reliable tool for monitoring the presence of xeno‐estrogens in the environment of these fish species. J. Exp. Zool. 305A, 2006. © 2006 Wiley‐Liss, Inc.</abstract><relatedItem type="host"><titleInfo><title>Journal of Experimental Zoology Part A: Comparative Experimental Biology</title></titleInfo><titleInfo type="abbreviated"><title>J. Exp. Zool.</title></titleInfo><genre type="journal">journal</genre><subject><genre>article-category</genre><topic>Reproductive Biology</topic></subject><identifier type="ISSN">1548-8969</identifier><identifier type="eISSN">1552-499X</identifier><identifier type="DOI">10.1002/(ISSN)1552-499X</identifier><identifier type="PublisherID">JEZ</identifier><part><date>2006</date><detail type="volume"><caption>vol.</caption><number>305A</number></detail><detail type="issue"><caption>no.</caption><number>7</number></detail><extent unit="pages"><start>576</start><end>593</end><total>18</total></extent></part></relatedItem><relatedItem type="preceding"><titleInfo><title>Journal of Experimental Zoology</title></titleInfo><identifier type="ISSN">0022-104X</identifier><identifier type="ISSN">1097-010X</identifier><part><date point="end">2004</date></part></relatedItem><identifier type="istex">93A5A5BE4EA64A2C273135E47EB93F1F05942A13</identifier><identifier type="DOI">10.1002/jez.a.290</identifier><identifier type="ArticleID">JEZ290</identifier><accessCondition type="use and reproduction" contentType="copyright">Copyright © 2006 Wiley‐Liss, Inc., A Wiley Company</accessCondition><recordInfo><recordContentSource>WILEY</recordContentSource><recordOrigin>Wiley Subscription Services, Inc., A Wiley Company</recordOrigin></recordInfo></mods></metadata><serie></serie></istex></record>Pour manipuler ce document sous Unix (Dilib)
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