Wood-Derived Estrogens: Studies in Vitro with Breast Cancer Cell Lines and in Vivo in Trout
Identifieur interne : 000E53 ( Istex/Corpus ); précédent : 000E52; suivant : 000E54Wood-Derived Estrogens: Studies in Vitro with Breast Cancer Cell Lines and in Vivo in Trout
Auteurs : Pirkko Mellanen ; Tiina Pet Nen ; Jyrki Lehtim Ki ; Sari M Kel ; Göran Bylund ; Bjarne Holmbom ; Erkki Mannila ; Aimo Oikari ; Risto SanttiSource :
- Toxicology and Applied Pharmacology [ 0041-008X ] ; 1996.
Abstract
The wood-derived compound, β-sitosterol (purity >90%), was shown to be estrogenic in fish. It induced the expression of the vitellogenin gene in the liver of juvenile and methyltestosterone-treated rainbow trout. Structural similarities to β-sitosterol notwithstanding, cholesterol, citrostadienol, β-sitostanol, and 5-androstene-3β,17β-diol, an estrogenic member of the androstenic steroid group, were inactive. An abietic acid mixture (37% abietic acid, 6% dehydroabietic acid, and a remainder of unknown compounds) showed slight hormonal activity in feed, but it was completely inactive when given intraperitoneally in implants. The estrogenic component of the abietic acid preparation was not identified. In addition to β-sitosterol and abietic acid, several other wood-derived compounds including betulin, isorhapontigenin, isorhapontin, and pinosylvin were estrogenic in breast cancer cells (MCF-7 or T-47D). However, betulin and pinosylvin, available in sufficient amounts forin vivotesting, did not induce the expression of the vitellogenin gene. Differences in the primary sequences of human and fish estrogen receptors (hormone as well as DNA-binding regions) or uptake and metabolism of the compounds may explain the discrepancy between the two estrogen bioassays. Wood-derived compounds such as β-sitosterol, present in pulp and paper mill effluents, may account for the weak estrogenicity of debarking effluent seen at the vitellogenin expression bioassay.
Url:
DOI: 10.1006/taap.1996.0046
Links to Exploration step
ISTEX:E4198E0BD1B69B0C8A19A5BB9DFE742E1F8019D7Le document en format XML
<record><TEI wicri:istexFullTextTei="biblStruct"><teiHeader><fileDesc><titleStmt><title xml:lang="en">Wood-Derived Estrogens: Studies in Vitro with Breast Cancer Cell Lines and in Vivo in Trout</title><author><name sortKey="Mellanen, Pirkko" sort="Mellanen, Pirkko" uniqKey="Mellanen P" first="Pirkko" last="Mellanen">Pirkko Mellanen</name><affiliation><mods:affiliation>Institute of Biomedicine, University of Turku, Turku, Finland</mods:affiliation></affiliation></author><author><name sortKey="Pet Nen, Tiina" sort="Pet Nen, Tiina" uniqKey="Pet Nen T" first="Tiina" last="Pet Nen">Tiina Pet Nen</name><affiliation><mods:affiliation>Departments of Biology and Environmental Sciences, University of Jyväskylä, Jyväskylä, Finland</mods:affiliation></affiliation></author><author><name sortKey="Lehtim Ki, Jyrki" sort="Lehtim Ki, Jyrki" uniqKey="Lehtim Ki J" first="Jyrki" last="Lehtim Ki">Jyrki Lehtim Ki</name><affiliation><mods:affiliation>Institute of Biomedicine, University of Turku, Turku, Finland</mods:affiliation></affiliation></author><author><name sortKey="M Kel, Sari" sort="M Kel, Sari" uniqKey="M Kel S" first="Sari" last="M Kel">Sari M Kel</name><affiliation><mods:affiliation>Institute of Biomedicine, University of Turku, Turku, Finland</mods:affiliation></affiliation></author><author><name sortKey="Bylund, Goran" sort="Bylund, Goran" uniqKey="Bylund G" first="Göran" last="Bylund">Göran Bylund</name><affiliation><mods:affiliation>Department of Biology, Åbo Akademi University, Turku, Finland</mods:affiliation></affiliation></author><author><name sortKey="Holmbom, Bjarne" sort="Holmbom, Bjarne" uniqKey="Holmbom B" first="Bjarne" last="Holmbom">Bjarne Holmbom</name><affiliation><mods:affiliation>Laboratory of Forest Products Chemistry, Åbo Akademi University, Turku, Finland</mods:affiliation></affiliation></author><author><name sortKey="Mannila, Erkki" sort="Mannila, Erkki" uniqKey="Mannila E" first="Erkki" last="Mannila">Erkki Mannila</name><affiliation><mods:affiliation>Department of Chemistry, University of Jyväskylä, Jyväskylä, Finland</mods:affiliation></affiliation></author><author><name sortKey="Oikari, Aimo" sort="Oikari, Aimo" uniqKey="Oikari A" first="Aimo" last="Oikari">Aimo Oikari</name><affiliation><mods:affiliation>Departments of Biology and Environmental Sciences, University of Jyväskylä, Jyväskylä, Finland</mods:affiliation></affiliation></author><author><name sortKey="Santti, Risto" sort="Santti, Risto" uniqKey="Santti R" first="Risto" last="Santti">Risto Santti</name><affiliation><mods:affiliation>Institute of Biomedicine, University of Turku, Turku, Finland</mods:affiliation></affiliation></author></titleStmt><publicationStmt><idno type="wicri:source">ISTEX</idno><idno type="RBID">ISTEX:E4198E0BD1B69B0C8A19A5BB9DFE742E1F8019D7</idno><date when="1996" year="1996">1996</date><idno type="doi">10.1006/taap.1996.0046</idno><idno type="url">https://api.istex.fr/document/E4198E0BD1B69B0C8A19A5BB9DFE742E1F8019D7/fulltext/pdf</idno><idno type="wicri:Area/Istex/Corpus">000E53</idno><idno type="wicri:explorRef" wicri:stream="Istex" wicri:step="Corpus" wicri:corpus="ISTEX">000E53</idno></publicationStmt><sourceDesc><biblStruct><analytic><title level="a" type="main" xml:lang="en">Wood-Derived Estrogens: Studies in Vitro with Breast Cancer Cell Lines and in Vivo in Trout</title><author><name sortKey="Mellanen, Pirkko" sort="Mellanen, Pirkko" uniqKey="Mellanen P" first="Pirkko" last="Mellanen">Pirkko Mellanen</name><affiliation><mods:affiliation>Institute of Biomedicine, University of Turku, Turku, Finland</mods:affiliation></affiliation></author><author><name sortKey="Pet Nen, Tiina" sort="Pet Nen, Tiina" uniqKey="Pet Nen T" first="Tiina" last="Pet Nen">Tiina Pet Nen</name><affiliation><mods:affiliation>Departments of Biology and Environmental Sciences, University of Jyväskylä, Jyväskylä, Finland</mods:affiliation></affiliation></author><author><name sortKey="Lehtim Ki, Jyrki" sort="Lehtim Ki, Jyrki" uniqKey="Lehtim Ki J" first="Jyrki" last="Lehtim Ki">Jyrki Lehtim Ki</name><affiliation><mods:affiliation>Institute of Biomedicine, University of Turku, Turku, Finland</mods:affiliation></affiliation></author><author><name sortKey="M Kel, Sari" sort="M Kel, Sari" uniqKey="M Kel S" first="Sari" last="M Kel">Sari M Kel</name><affiliation><mods:affiliation>Institute of Biomedicine, University of Turku, Turku, Finland</mods:affiliation></affiliation></author><author><name sortKey="Bylund, Goran" sort="Bylund, Goran" uniqKey="Bylund G" first="Göran" last="Bylund">Göran Bylund</name><affiliation><mods:affiliation>Department of Biology, Åbo Akademi University, Turku, Finland</mods:affiliation></affiliation></author><author><name sortKey="Holmbom, Bjarne" sort="Holmbom, Bjarne" uniqKey="Holmbom B" first="Bjarne" last="Holmbom">Bjarne Holmbom</name><affiliation><mods:affiliation>Laboratory of Forest Products Chemistry, Åbo Akademi University, Turku, Finland</mods:affiliation></affiliation></author><author><name sortKey="Mannila, Erkki" sort="Mannila, Erkki" uniqKey="Mannila E" first="Erkki" last="Mannila">Erkki Mannila</name><affiliation><mods:affiliation>Department of Chemistry, University of Jyväskylä, Jyväskylä, Finland</mods:affiliation></affiliation></author><author><name sortKey="Oikari, Aimo" sort="Oikari, Aimo" uniqKey="Oikari A" first="Aimo" last="Oikari">Aimo Oikari</name><affiliation><mods:affiliation>Departments of Biology and Environmental Sciences, University of Jyväskylä, Jyväskylä, Finland</mods:affiliation></affiliation></author><author><name sortKey="Santti, Risto" sort="Santti, Risto" uniqKey="Santti R" first="Risto" last="Santti">Risto Santti</name><affiliation><mods:affiliation>Institute of Biomedicine, University of Turku, Turku, Finland</mods:affiliation></affiliation></author></analytic><monogr></monogr><series><title level="j">Toxicology and Applied Pharmacology</title><title level="j" type="abbrev">YTAAP</title><idno type="ISSN">0041-008X</idno><imprint><publisher>ELSEVIER</publisher><date type="published" when="1996">1996</date><biblScope unit="volume">136</biblScope><biblScope unit="issue">2</biblScope><biblScope unit="page" from="381">381</biblScope><biblScope unit="page" to="388">388</biblScope></imprint><idno type="ISSN">0041-008X</idno></series><idno type="istex">E4198E0BD1B69B0C8A19A5BB9DFE742E1F8019D7</idno><idno type="DOI">10.1006/taap.1996.0046</idno><idno type="PII">S0041-008X(96)90046-0</idno></biblStruct></sourceDesc><seriesStmt><idno type="ISSN">0041-008X</idno></seriesStmt></fileDesc><profileDesc><textClass></textClass><langUsage><language ident="en">en</language></langUsage></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">The wood-derived compound, β-sitosterol (purity >90%), was shown to be estrogenic in fish. It induced the expression of the vitellogenin gene in the liver of juvenile and methyltestosterone-treated rainbow trout. Structural similarities to β-sitosterol notwithstanding, cholesterol, citrostadienol, β-sitostanol, and 5-androstene-3β,17β-diol, an estrogenic member of the androstenic steroid group, were inactive. An abietic acid mixture (37% abietic acid, 6% dehydroabietic acid, and a remainder of unknown compounds) showed slight hormonal activity in feed, but it was completely inactive when given intraperitoneally in implants. The estrogenic component of the abietic acid preparation was not identified. In addition to β-sitosterol and abietic acid, several other wood-derived compounds including betulin, isorhapontigenin, isorhapontin, and pinosylvin were estrogenic in breast cancer cells (MCF-7 or T-47D). However, betulin and pinosylvin, available in sufficient amounts forin vivotesting, did not induce the expression of the vitellogenin gene. Differences in the primary sequences of human and fish estrogen receptors (hormone as well as DNA-binding regions) or uptake and metabolism of the compounds may explain the discrepancy between the two estrogen bioassays. Wood-derived compounds such as β-sitosterol, present in pulp and paper mill effluents, may account for the weak estrogenicity of debarking effluent seen at the vitellogenin expression bioassay.</div></front></TEI><istex><corpusName>elsevier</corpusName><author><json:item><name>Pirkko Mellanen</name><affiliations><json:string>Institute of Biomedicine, University of Turku, Turku, Finland</json:string></affiliations></json:item><json:item><name>Tiina Petänen</name><affiliations><json:string>Departments of Biology and Environmental Sciences, University of Jyväskylä, Jyväskylä, Finland</json:string></affiliations></json:item><json:item><name>Jyrki Lehtimäki</name><affiliations><json:string>Institute of Biomedicine, University of Turku, Turku, Finland</json:string></affiliations></json:item><json:item><name>Sari Mäkelä</name><affiliations><json:string>Institute of Biomedicine, University of Turku, Turku, Finland</json:string></affiliations></json:item><json:item><name>Göran Bylund</name><affiliations><json:string>Department of Biology, Åbo Akademi University, Turku, Finland</json:string></affiliations></json:item><json:item><name>Bjarne Holmbom</name><affiliations><json:string>Laboratory of Forest Products Chemistry, Åbo Akademi University, Turku, Finland</json:string></affiliations></json:item><json:item><name>Erkki Mannila</name><affiliations><json:string>Department of Chemistry, University of Jyväskylä, Jyväskylä, Finland</json:string></affiliations></json:item><json:item><name>Aimo Oikari</name><affiliations><json:string>Departments of Biology and Environmental Sciences, University of Jyväskylä, Jyväskylä, Finland</json:string></affiliations></json:item><json:item><name>Risto Santti</name><affiliations><json:string>Institute of Biomedicine, University of Turku, Turku, Finland</json:string></affiliations></json:item></author><language><json:string>eng</json:string></language><originalGenre><json:string>Full-length article</json:string></originalGenre><abstract>The wood-derived compound, β-sitosterol (purity >90%), was shown to be estrogenic in fish. It induced the expression of the vitellogenin gene in the liver of juvenile and methyltestosterone-treated rainbow trout. Structural similarities to β-sitosterol notwithstanding, cholesterol, citrostadienol, β-sitostanol, and 5-androstene-3β,17β-diol, an estrogenic member of the androstenic steroid group, were inactive. An abietic acid mixture (37% abietic acid, 6% dehydroabietic acid, and a remainder of unknown compounds) showed slight hormonal activity in feed, but it was completely inactive when given intraperitoneally in implants. The estrogenic component of the abietic acid preparation was not identified. In addition to β-sitosterol and abietic acid, several other wood-derived compounds including betulin, isorhapontigenin, isorhapontin, and pinosylvin were estrogenic in breast cancer cells (MCF-7 or T-47D). However, betulin and pinosylvin, available in sufficient amounts forin vivotesting, did not induce the expression of the vitellogenin gene. Differences in the primary sequences of human and fish estrogen receptors (hormone as well as DNA-binding regions) or uptake and metabolism of the compounds may explain the discrepancy between the two estrogen bioassays. Wood-derived compounds such as β-sitosterol, present in pulp and paper mill effluents, may account for the weak estrogenicity of debarking effluent seen at the vitellogenin expression bioassay.</abstract><qualityIndicators><score>7.224</score><pdfVersion>1.1</pdfVersion><pdfPageSize>547 x 726 pts</pdfPageSize><refBibsNative>false</refBibsNative><keywordCount>0</keywordCount><abstractCharCount>1469</abstractCharCount><pdfWordCount>4848</pdfWordCount><pdfCharCount>31142</pdfCharCount><pdfPageCount>8</pdfPageCount><abstractWordCount>198</abstractWordCount></qualityIndicators><title>Wood-Derived Estrogens: Studies in Vitro with Breast Cancer Cell Lines and in Vivo in Trout</title><pii><json:string>S0041-008X(96)90046-0</json:string></pii><genre><json:string>research-article</json:string></genre><host><volume>136</volume><pii><json:string>S0041-008X(00)X0112-3</json:string></pii><pages><last>388</last><first>381</first></pages><issn><json:string>0041-008X</json:string></issn><issue>2</issue><genre><json:string>journal</json:string></genre><language><json:string>unknown</json:string></language><title>Toxicology and Applied Pharmacology</title><publicationDate>1996</publicationDate></host><categories><wos><json:string>science</json:string><json:string>toxicology</json:string><json:string>pharmacology & pharmacy</json:string></wos><scienceMetrix><json:string>health sciences</json:string><json:string>biomedical research</json:string><json:string>toxicology</json:string></scienceMetrix></categories><publicationDate>1996</publicationDate><copyrightDate>1996</copyrightDate><doi><json:string>10.1006/taap.1996.0046</json:string></doi><id>E4198E0BD1B69B0C8A19A5BB9DFE742E1F8019D7</id><score>0.028164484</score><fulltext><json:item><extension>pdf</extension><original>true</original><mimetype>application/pdf</mimetype><uri>https://api.istex.fr/document/E4198E0BD1B69B0C8A19A5BB9DFE742E1F8019D7/fulltext/pdf</uri></json:item><json:item><extension>zip</extension><original>false</original><mimetype>application/zip</mimetype><uri>https://api.istex.fr/document/E4198E0BD1B69B0C8A19A5BB9DFE742E1F8019D7/fulltext/zip</uri></json:item><istex:fulltextTEI uri="https://api.istex.fr/document/E4198E0BD1B69B0C8A19A5BB9DFE742E1F8019D7/fulltext/tei"><teiHeader><fileDesc><titleStmt><title level="a" type="main" xml:lang="en">Wood-Derived Estrogens: Studies in Vitro with Breast Cancer Cell Lines and in Vivo in Trout</title><respStmt><resp>Références bibliographiques récupérées via GROBID</resp><name resp="ISTEX-API">ISTEX-API (INIST-CNRS)</name></respStmt><respStmt><resp>Références bibliographiques récupérées via GROBID</resp><name resp="ISTEX-API">ISTEX-API (INIST-CNRS)</name></respStmt><respStmt><resp>Références bibliographiques récupérées via GROBID</resp><name resp="ISTEX-API">ISTEX-API (INIST-CNRS)</name></respStmt></titleStmt><publicationStmt><authority>ISTEX</authority><publisher>ELSEVIER</publisher><availability><p>©1996 Academic Press</p></availability><date>1996</date></publicationStmt><notesStmt><note type="content">Section title: Regular Article</note></notesStmt><sourceDesc><biblStruct type="inbook"><analytic><title level="a" type="main" xml:lang="en">Wood-Derived Estrogens: Studies in Vitro with Breast Cancer Cell Lines and in Vivo in Trout</title><author xml:id="author-1"><persName><forename type="first">Pirkko</forename><surname>Mellanen</surname></persName><affiliation>Institute of Biomedicine, University of Turku, Turku, Finland</affiliation></author><author xml:id="author-2"><persName><forename type="first">Tiina</forename><surname>Petänen</surname></persName><affiliation>Departments of Biology and Environmental Sciences, University of Jyväskylä, Jyväskylä, Finland</affiliation></author><author xml:id="author-3"><persName><forename type="first">Jyrki</forename><surname>Lehtimäki</surname></persName><affiliation>Institute of Biomedicine, University of Turku, Turku, Finland</affiliation></author><author xml:id="author-4"><persName><forename type="first">Sari</forename><surname>Mäkelä</surname></persName><affiliation>Institute of Biomedicine, University of Turku, Turku, Finland</affiliation></author><author xml:id="author-5"><persName><forename type="first">Göran</forename><surname>Bylund</surname></persName><affiliation>Department of Biology, Åbo Akademi University, Turku, Finland</affiliation></author><author xml:id="author-6"><persName><forename type="first">Bjarne</forename><surname>Holmbom</surname></persName><affiliation>Laboratory of Forest Products Chemistry, Åbo Akademi University, Turku, Finland</affiliation></author><author xml:id="author-7"><persName><forename type="first">Erkki</forename><surname>Mannila</surname></persName><affiliation>Department of Chemistry, University of Jyväskylä, Jyväskylä, Finland</affiliation></author><author xml:id="author-8"><persName><forename type="first">Aimo</forename><surname>Oikari</surname></persName><affiliation>Departments of Biology and Environmental Sciences, University of Jyväskylä, Jyväskylä, Finland</affiliation></author><author xml:id="author-9"><persName><forename type="first">Risto</forename><surname>Santti</surname></persName><affiliation>Institute of Biomedicine, University of Turku, Turku, Finland</affiliation></author></analytic><monogr><title level="j">Toxicology and Applied Pharmacology</title><title level="j" type="abbrev">YTAAP</title><idno type="pISSN">0041-008X</idno><idno type="PII">S0041-008X(00)X0112-3</idno><imprint><publisher>ELSEVIER</publisher><date type="published" when="1996"></date><biblScope unit="volume">136</biblScope><biblScope unit="issue">2</biblScope><biblScope unit="page" from="381">381</biblScope><biblScope unit="page" to="388">388</biblScope></imprint></monogr><idno type="istex">E4198E0BD1B69B0C8A19A5BB9DFE742E1F8019D7</idno><idno type="DOI">10.1006/taap.1996.0046</idno><idno type="PII">S0041-008X(96)90046-0</idno></biblStruct></sourceDesc></fileDesc><profileDesc><creation><date>1996</date></creation><langUsage><language ident="en">en</language></langUsage><abstract xml:lang="en"><p>The wood-derived compound, β-sitosterol (purity >90%), was shown to be estrogenic in fish. It induced the expression of the vitellogenin gene in the liver of juvenile and methyltestosterone-treated rainbow trout. Structural similarities to β-sitosterol notwithstanding, cholesterol, citrostadienol, β-sitostanol, and 5-androstene-3β,17β-diol, an estrogenic member of the androstenic steroid group, were inactive. An abietic acid mixture (37% abietic acid, 6% dehydroabietic acid, and a remainder of unknown compounds) showed slight hormonal activity in feed, but it was completely inactive when given intraperitoneally in implants. The estrogenic component of the abietic acid preparation was not identified. In addition to β-sitosterol and abietic acid, several other wood-derived compounds including betulin, isorhapontigenin, isorhapontin, and pinosylvin were estrogenic in breast cancer cells (MCF-7 or T-47D). However, betulin and pinosylvin, available in sufficient amounts forin vivotesting, did not induce the expression of the vitellogenin gene. Differences in the primary sequences of human and fish estrogen receptors (hormone as well as DNA-binding regions) or uptake and metabolism of the compounds may explain the discrepancy between the two estrogen bioassays. Wood-derived compounds such as β-sitosterol, present in pulp and paper mill effluents, may account for the weak estrogenicity of debarking effluent seen at the vitellogenin expression bioassay.</p></abstract></profileDesc><revisionDesc><change when="1996">Published</change><change xml:id="refBibs-istex" who="#ISTEX-API" when="2016-12-20">References added</change><change xml:id="refBibs-istex" who="#ISTEX-API" when="2017-01-13">References added</change><change xml:id="refBibs-istex" who="#ISTEX-API" when="2017-01-16">References added</change></revisionDesc></teiHeader></istex:fulltextTEI><json:item><extension>txt</extension><original>false</original><mimetype>text/plain</mimetype><uri>https://api.istex.fr/document/E4198E0BD1B69B0C8A19A5BB9DFE742E1F8019D7/fulltext/txt</uri></json:item></fulltext><metadata><istex:metadataXml wicri:clean="Elsevier converted-article found"><istex:xmlDeclaration>version="1.0" encoding="utf-8"</istex:xmlDeclaration><istex:docType PUBLIC="-//ES//DTD journal article DTD version 4.5.2//EN//XML" URI="art452.dtd" name="istex:docType"></istex:docType><istex:document><converted-article version="4.5.2" docsubtype="fla" xml:lang="en"><item-info><jid>YTAAP</jid><aid>90046</aid><ce:pii>S0041-008X(96)90046-0</ce:pii><ce:doi>10.1006/taap.1996.0046</ce:doi><ce:copyright type="full-transfer" year="1996">Academic Press</ce:copyright></item-info><head><ce:dochead><ce:textfn>Regular Article</ce:textfn></ce:dochead><ce:title>Wood-Derived Estrogens: Studies<ce:italic>in Vitro</ce:italic>with Breast Cancer Cell Lines and<ce:italic>in Vivo</ce:italic>in Trout</ce:title><ce:author-group><ce:author><ce:given-name>Pirkko</ce:given-name><ce:surname>Mellanen</ce:surname><ce:cross-ref refid="A1"><ce:sup>a</ce:sup></ce:cross-ref></ce:author><ce:author><ce:given-name>Tiina</ce:given-name><ce:surname>Petänen</ce:surname><ce:cross-ref refid="A2"><ce:sup>b</ce:sup></ce:cross-ref></ce:author><ce:author><ce:given-name>Jyrki</ce:given-name><ce:surname>Lehtimäki</ce:surname><ce:cross-ref refid="A1"><ce:sup>a</ce:sup></ce:cross-ref></ce:author><ce:author><ce:given-name>Sari</ce:given-name><ce:surname>Mäkelä</ce:surname><ce:cross-ref refid="A1"><ce:sup>a</ce:sup></ce:cross-ref></ce:author><ce:author><ce:given-name>Göran</ce:given-name><ce:surname>Bylund</ce:surname><ce:cross-ref refid="A3"><ce:sup>c</ce:sup></ce:cross-ref></ce:author><ce:author><ce:given-name>Bjarne</ce:given-name><ce:surname>Holmbom</ce:surname><ce:cross-ref refid="A4"><ce:sup>d</ce:sup></ce:cross-ref></ce:author><ce:author><ce:given-name>Erkki</ce:given-name><ce:surname>Mannila</ce:surname><ce:cross-ref refid="A5"><ce:sup>e</ce:sup></ce:cross-ref></ce:author><ce:author><ce:given-name>Aimo</ce:given-name><ce:surname>Oikari</ce:surname><ce:cross-ref refid="A2"><ce:sup>b</ce:sup></ce:cross-ref></ce:author><ce:author><ce:given-name>Risto</ce:given-name><ce:surname>Santti</ce:surname><ce:cross-ref refid="A1"><ce:sup>a</ce:sup></ce:cross-ref></ce:author><ce:affiliation id="A1"><ce:label>a</ce:label><ce:textfn>Institute of Biomedicine, University of Turku, Turku, Finland</ce:textfn></ce:affiliation><ce:affiliation id="A2"><ce:label>b</ce:label><ce:textfn>Departments of Biology and Environmental Sciences, University of Jyväskylä, Jyväskylä, Finland</ce:textfn></ce:affiliation><ce:affiliation id="A5"><ce:label>e</ce:label><ce:textfn>Department of Chemistry, University of Jyväskylä, Jyväskylä, Finland</ce:textfn></ce:affiliation><ce:affiliation id="A3"><ce:label>c</ce:label><ce:textfn>Department of Biology, Åbo Akademi University, Turku, Finland</ce:textfn></ce:affiliation><ce:affiliation id="A4"><ce:label>d</ce:label><ce:textfn>Laboratory of Forest Products Chemistry, Åbo Akademi University, Turku, Finland</ce:textfn></ce:affiliation></ce:author-group><ce:date-received day="12" month="7" year="1995"></ce:date-received><ce:date-accepted day="11" month="11" year="1995"></ce:date-accepted><ce:abstract><ce:section-title>Abstract</ce:section-title><ce:abstract-sec><ce:simple-para>The wood-derived compound, β-sitosterol (purity >90%), was shown to be estrogenic in fish. It induced the expression of the vitellogenin gene in the liver of juvenile and methyltestosterone-treated rainbow trout. Structural similarities to β-sitosterol notwithstanding, cholesterol, citrostadienol, β-sitostanol, and 5-androstene-3β,17β-diol, an estrogenic member of the androstenic steroid group, were inactive. An abietic acid mixture (37% abietic acid, 6% dehydroabietic acid, and a remainder of unknown compounds) showed slight hormonal activity in feed, but it was completely inactive when given intraperitoneally in implants. The estrogenic component of the abietic acid preparation was not identified. In addition to β-sitosterol and abietic acid, several other wood-derived compounds including betulin, isorhapontigenin, isorhapontin, and pinosylvin were estrogenic in breast cancer cells (MCF-7 or T-47D). However, betulin and pinosylvin, available in sufficient amounts for<ce:italic>in vivo</ce:italic>testing, did not induce the expression of the vitellogenin gene. Differences in the primary sequences of human and fish estrogen receptors (hormone as well as DNA-binding regions) or uptake and metabolism of the compounds may explain the discrepancy between the two estrogen bioassays. Wood-derived compounds such as β-sitosterol, present in pulp and paper mill effluents, may account for the weak estrogenicity of debarking effluent seen at the vitellogenin expression bioassay.</ce:simple-para></ce:abstract-sec></ce:abstract></head></converted-article></istex:document></istex:metadataXml><mods version="3.6"><titleInfo lang="en"><title>Wood-Derived Estrogens: Studies in Vitro with Breast Cancer Cell Lines and in Vivo in Trout</title></titleInfo><titleInfo type="alternative" lang="en" contentType="CDATA"><title>Wood-Derived Estrogens: Studies</title></titleInfo><name type="personal"><namePart type="given">Pirkko</namePart><namePart type="family">Mellanen</namePart><affiliation>Institute of Biomedicine, University of Turku, Turku, Finland</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">Tiina</namePart><namePart type="family">Petänen</namePart><affiliation>Departments of Biology and Environmental Sciences, University of Jyväskylä, Jyväskylä, Finland</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">Jyrki</namePart><namePart type="family">Lehtimäki</namePart><affiliation>Institute of Biomedicine, University of Turku, Turku, Finland</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">Sari</namePart><namePart type="family">Mäkelä</namePart><affiliation>Institute of Biomedicine, University of Turku, Turku, Finland</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">Göran</namePart><namePart type="family">Bylund</namePart><affiliation>Department of Biology, Åbo Akademi University, Turku, Finland</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">Bjarne</namePart><namePart type="family">Holmbom</namePart><affiliation>Laboratory of Forest Products Chemistry, Åbo Akademi University, Turku, Finland</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">Erkki</namePart><namePart type="family">Mannila</namePart><affiliation>Department of Chemistry, University of Jyväskylä, Jyväskylä, Finland</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">Aimo</namePart><namePart type="family">Oikari</namePart><affiliation>Departments of Biology and Environmental Sciences, University of Jyväskylä, Jyväskylä, Finland</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">Risto</namePart><namePart type="family">Santti</namePart><affiliation>Institute of Biomedicine, University of Turku, Turku, Finland</affiliation><role><roleTerm type="text">author</roleTerm></role></name><typeOfResource>text</typeOfResource><genre type="research-article" displayLabel="Full-length article"></genre><originInfo><publisher>ELSEVIER</publisher><dateIssued encoding="w3cdtf">1996</dateIssued><copyrightDate encoding="w3cdtf">1996</copyrightDate></originInfo><language><languageTerm type="code" authority="iso639-2b">eng</languageTerm><languageTerm type="code" authority="rfc3066">en</languageTerm></language><physicalDescription><internetMediaType>text/html</internetMediaType></physicalDescription><abstract lang="en">The wood-derived compound, β-sitosterol (purity >90%), was shown to be estrogenic in fish. It induced the expression of the vitellogenin gene in the liver of juvenile and methyltestosterone-treated rainbow trout. Structural similarities to β-sitosterol notwithstanding, cholesterol, citrostadienol, β-sitostanol, and 5-androstene-3β,17β-diol, an estrogenic member of the androstenic steroid group, were inactive. An abietic acid mixture (37% abietic acid, 6% dehydroabietic acid, and a remainder of unknown compounds) showed slight hormonal activity in feed, but it was completely inactive when given intraperitoneally in implants. The estrogenic component of the abietic acid preparation was not identified. In addition to β-sitosterol and abietic acid, several other wood-derived compounds including betulin, isorhapontigenin, isorhapontin, and pinosylvin were estrogenic in breast cancer cells (MCF-7 or T-47D). However, betulin and pinosylvin, available in sufficient amounts forin vivotesting, did not induce the expression of the vitellogenin gene. Differences in the primary sequences of human and fish estrogen receptors (hormone as well as DNA-binding regions) or uptake and metabolism of the compounds may explain the discrepancy between the two estrogen bioassays. Wood-derived compounds such as β-sitosterol, present in pulp and paper mill effluents, may account for the weak estrogenicity of debarking effluent seen at the vitellogenin expression bioassay.</abstract><note type="content">Section title: Regular Article</note><relatedItem type="host"><titleInfo><title>Toxicology and Applied Pharmacology</title></titleInfo><titleInfo type="abbreviated"><title>YTAAP</title></titleInfo><genre type="journal">journal</genre><originInfo><dateIssued encoding="w3cdtf">199602</dateIssued></originInfo><identifier type="ISSN">0041-008X</identifier><identifier type="PII">S0041-008X(00)X0112-3</identifier><part><date>199602</date><detail type="volume"><number>136</number><caption>vol.</caption></detail><detail type="issue"><number>2</number><caption>no.</caption></detail><extent unit="issue pages"><start>211</start><end>397</end></extent><extent unit="pages"><start>381</start><end>388</end></extent></part></relatedItem><identifier type="istex">E4198E0BD1B69B0C8A19A5BB9DFE742E1F8019D7</identifier><identifier type="DOI">10.1006/taap.1996.0046</identifier><identifier type="PII">S0041-008X(96)90046-0</identifier><accessCondition type="use and reproduction" contentType="copyright">©1996 Academic Press</accessCondition><recordInfo><recordContentSource>ELSEVIER</recordContentSource><recordOrigin>Academic Press, ©1996</recordOrigin></recordInfo></mods></metadata><serie></serie></istex></record>Pour manipuler ce document sous Unix (Dilib)
EXPLOR_STEP=$WICRI_ROOT/Wicri/Eau/explor/EsturgeonV1/Data/Istex/Corpus
HfdSelect -h $EXPLOR_STEP/biblio.hfd -nk 000E53 | SxmlIndent | more
Ou
HfdSelect -h $EXPLOR_AREA/Data/Istex/Corpus/biblio.hfd -nk 000E53 | SxmlIndent | more
Pour mettre un lien sur cette page dans le réseau Wicri
{{Explor lien
|wiki= Wicri/Eau
|area= EsturgeonV1
|flux= Istex
|étape= Corpus
|type= RBID
|clé= ISTEX:E4198E0BD1B69B0C8A19A5BB9DFE742E1F8019D7
|texte= Wood-Derived Estrogens: Studies in Vitro with Breast Cancer Cell Lines and in Vivo in Trout
}}
| This area was generated with Dilib version V0.6.27. |  |