Neuropeptide regulation of feeding in catfish, Ictalurus punctatus : a role for glucagon-like peptide-1 (GLP-1)?
Identifieur interne : 000E22 ( Istex/Corpus ); précédent : 000E21; suivant : 000E23Neuropeptide regulation of feeding in catfish, Ictalurus punctatus : a role for glucagon-like peptide-1 (GLP-1)?
Auteurs : Jeffrey T. Silverstein ; Vera M. Bondareva ; Jill B. K. Leonard ; Erika M. PlisetskayaSource :
- Comparative Biochemistry and Physiology, Part B: Biochemistry and Molecular Biology [ 1096-4959 ] ; 2001.
English descriptors
- KwdEn :
Abstract
Glucagon-like peptide 1 is a compound known to cause reduced food intake in mammals, though its action on feed intake in fish is unknown. The clear differences in the effects of GLP-1 on mammalian and teleostean glucose homeostasis suggest that we cannot assume a similar action of GLP-1 on feeding in mammals and fish. In this study the effects and specificity of centrally administered GLP-1 on feed intake were examined. It was demonstrated that intracerebroventricular (ICV) injection of glucagon-like peptide 1 (GLP-1) in the channel catfish (Ictalurus punctatus) is a potent inhibitor of feed intake with a dose of 0.25 ng g−1 body wt. reducing feed intake by 50%. The weak response to intraperitoneal (i.p.) and intravenous (i.v.) injection treatments with GLP-1 suggests the major effects on feed intake are centrally mediated. GLP-1 action on feed intake was not antagonized by ICV injection of exendin9–39. Immunoneutralization of GLP-1 by ICV injection of antisalmon GLP-1 antisera did not affect feed intake over 48 h, while ICV injection of GLP-1 at a dose of 30 ng g−1 body wt. reduced feed intake for over 20 h. Additionally, there is some evidence that GLP-1 caused gastric evacuation. We conclude that GLP-1 is a potent inhibitor of feeding in fish, but its involvement in feed intake regulation under physiological conditions remains to be clarified.
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DOI: 10.1016/S1096-4959(01)00357-8
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Bondareva</name><affiliation><mods:affiliation>Sechenov Institute of Evolutionary Physiology and Biochemistry, St. Petersburg, Russia</mods:affiliation></affiliation></author><author><name sortKey="Leonard, Jill B K" sort="Leonard, Jill B K" uniqKey="Leonard J" first="Jill B. K." last="Leonard">Jill B. K. Leonard</name><affiliation><mods:affiliation>USDA, ARS, Catfish Genetics Research Unit, Thad Cochran National Warmwater Aquaculture Center, Stoneville, MS, 38776, USA</mods:affiliation></affiliation></author><author><name sortKey="Plisetskaya, Erika M" sort="Plisetskaya, Erika M" uniqKey="Plisetskaya E" first="Erika M." last="Plisetskaya">Erika M. 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Leonard</name><affiliation><mods:affiliation>USDA, ARS, Catfish Genetics Research Unit, Thad Cochran National Warmwater Aquaculture Center, Stoneville, MS, 38776, USA</mods:affiliation></affiliation></author><author><name sortKey="Plisetskaya, Erika M" sort="Plisetskaya, Erika M" uniqKey="Plisetskaya E" first="Erika M." last="Plisetskaya">Erika M. Plisetskaya</name><affiliation><mods:affiliation>University of Washington, School of Fisheries, Seattle, WA 98195 USA</mods:affiliation></affiliation></author></analytic><monogr></monogr><series><title level="j">Comparative Biochemistry and Physiology, Part B: Biochemistry and Molecular Biology</title><title level="j" type="abbrev">CBB</title><idno type="ISSN">1096-4959</idno><imprint><publisher>ELSEVIER</publisher><date type="published" when="2001">2001</date><biblScope unit="volume">129</biblScope><biblScope unit="issue">2–3</biblScope><biblScope unit="page" from="623">623</biblScope><biblScope unit="page" to="631">631</biblScope></imprint><idno type="ISSN">1096-4959</idno></series><idno type="istex">55329048E08E1F6C15C86FA78D0F4652CEDF10FD</idno><idno type="DOI">10.1016/S1096-4959(01)00357-8</idno><idno type="PII">S1096-4959(01)00357-8</idno></biblStruct></sourceDesc><seriesStmt><idno type="ISSN">1096-4959</idno></seriesStmt></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Catfish</term><term>Exendin9–39</term><term>Feed intake regulation</term><term>Feeding</term><term>Glucagon-like peptide-1</term><term>Ictalurus punctatus</term><term>Immunoneutralization</term><term>Intracerebroventricular injection</term></keywords></textClass><langUsage><language ident="en">en</language></langUsage></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Glucagon-like peptide 1 is a compound known to cause reduced food intake in mammals, though its action on feed intake in fish is unknown. The clear differences in the effects of GLP-1 on mammalian and teleostean glucose homeostasis suggest that we cannot assume a similar action of GLP-1 on feeding in mammals and fish. In this study the effects and specificity of centrally administered GLP-1 on feed intake were examined. It was demonstrated that intracerebroventricular (ICV) injection of glucagon-like peptide 1 (GLP-1) in the channel catfish (Ictalurus punctatus) is a potent inhibitor of feed intake with a dose of 0.25 ng g−1 body wt. reducing feed intake by 50%. The weak response to intraperitoneal (i.p.) and intravenous (i.v.) injection treatments with GLP-1 suggests the major effects on feed intake are centrally mediated. GLP-1 action on feed intake was not antagonized by ICV injection of exendin9–39. Immunoneutralization of GLP-1 by ICV injection of antisalmon GLP-1 antisera did not affect feed intake over 48 h, while ICV injection of GLP-1 at a dose of 30 ng g−1 body wt. reduced feed intake for over 20 h. Additionally, there is some evidence that GLP-1 caused gastric evacuation. We conclude that GLP-1 is a potent inhibitor of feeding in fish, but its involvement in feed intake regulation under physiological conditions remains to be clarified.</div></front></TEI><istex><corpusName>elsevier</corpusName><author><json:item><name>Jeffrey T. Silverstein</name><affiliations><json:string>E-mail: jsilvers@ars.usda.gov</json:string><json:string>USDA, ARS, Catfish Genetics Research Unit, Thad Cochran National Warmwater Aquaculture Center, Stoneville, MS, 38776, USA</json:string></affiliations></json:item><json:item><name>Vera M. Bondareva</name><affiliations><json:string>Sechenov Institute of Evolutionary Physiology and Biochemistry, St. Petersburg, Russia</json:string></affiliations></json:item><json:item><name>Jill B.K. Leonard</name><affiliations><json:string>USDA, ARS, Catfish Genetics Research Unit, Thad Cochran National Warmwater Aquaculture Center, Stoneville, MS, 38776, USA</json:string></affiliations></json:item><json:item><name>Erika M. Plisetskaya</name><affiliations><json:string>University of Washington, School of Fisheries, Seattle, WA 98195 USA</json:string></affiliations></json:item></author><subject><json:item><lang><json:string>eng</json:string></lang><value>Feeding</value></json:item><json:item><lang><json:string>eng</json:string></lang><value>Feed intake regulation</value></json:item><json:item><lang><json:string>eng</json:string></lang><value>Glucagon-like peptide-1</value></json:item><json:item><lang><json:string>eng</json:string></lang><value>Intracerebroventricular injection</value></json:item><json:item><lang><json:string>eng</json:string></lang><value>Exendin9–39</value></json:item><json:item><lang><json:string>eng</json:string></lang><value>Immunoneutralization</value></json:item><json:item><lang><json:string>eng</json:string></lang><value>Catfish</value></json:item><json:item><lang><json:string>eng</json:string></lang><value>Ictalurus punctatus</value></json:item></subject><language><json:string>eng</json:string></language><originalGenre><json:string>Full-length article</json:string></originalGenre><abstract>Glucagon-like peptide 1 is a compound known to cause reduced food intake in mammals, though its action on feed intake in fish is unknown. The clear differences in the effects of GLP-1 on mammalian and teleostean glucose homeostasis suggest that we cannot assume a similar action of GLP-1 on feeding in mammals and fish. In this study the effects and specificity of centrally administered GLP-1 on feed intake were examined. It was demonstrated that intracerebroventricular (ICV) injection of glucagon-like peptide 1 (GLP-1) in the channel catfish (Ictalurus punctatus) is a potent inhibitor of feed intake with a dose of 0.25 ng g−1 body wt. reducing feed intake by 50%. The weak response to intraperitoneal (i.p.) and intravenous (i.v.) injection treatments with GLP-1 suggests the major effects on feed intake are centrally mediated. GLP-1 action on feed intake was not antagonized by ICV injection of exendin9–39. Immunoneutralization of GLP-1 by ICV injection of antisalmon GLP-1 antisera did not affect feed intake over 48 h, while ICV injection of GLP-1 at a dose of 30 ng g−1 body wt. reduced feed intake for over 20 h. Additionally, there is some evidence that GLP-1 caused gastric evacuation. We conclude that GLP-1 is a potent inhibitor of feeding in fish, but its involvement in feed intake regulation under physiological conditions remains to be clarified.</abstract><qualityIndicators><score>7.145</score><pdfVersion>1.2</pdfVersion><pdfPageSize>595 x 794 pts</pdfPageSize><refBibsNative>true</refBibsNative><keywordCount>8</keywordCount><abstractCharCount>1368</abstractCharCount><pdfWordCount>4505</pdfWordCount><pdfCharCount>26401</pdfCharCount><pdfPageCount>9</pdfPageCount><abstractWordCount>220</abstractWordCount></qualityIndicators><title>Neuropeptide regulation of feeding in catfish, Ictalurus punctatus : a role for glucagon-like peptide-1 (GLP-1)?</title><pii><json:string>S1096-4959(01)00357-8</json:string></pii><genre><json:string>research-article</json:string></genre><host><volume>129</volume><pii><json:string>S1096-4959(00)X0006-1</json:string></pii><pages><last>631</last><first>623</first></pages><issn><json:string>1096-4959</json:string></issn><issue>2–3</issue><genre><json:string>journal</json:string></genre><language><json:string>unknown</json:string></language><title>Comparative Biochemistry and Physiology, Part B: Biochemistry and Molecular Biology</title><publicationDate>2001</publicationDate></host><categories><wos><json:string>science</json:string><json:string>zoology</json:string><json:string>biochemistry & molecular biology</json:string></wos><scienceMetrix><json:string>health sciences</json:string><json:string>biomedical research</json:string><json:string>biochemistry & molecular biology</json:string></scienceMetrix></categories><publicationDate>2001</publicationDate><copyrightDate>2001</copyrightDate><doi><json:string>10.1016/S1096-4959(01)00357-8</json:string></doi><id>55329048E08E1F6C15C86FA78D0F4652CEDF10FD</id><score>0.020502236</score><fulltext><json:item><extension>pdf</extension><original>true</original><mimetype>application/pdf</mimetype><uri>https://api.istex.fr/document/55329048E08E1F6C15C86FA78D0F4652CEDF10FD/fulltext/pdf</uri></json:item><json:item><extension>zip</extension><original>false</original><mimetype>application/zip</mimetype><uri>https://api.istex.fr/document/55329048E08E1F6C15C86FA78D0F4652CEDF10FD/fulltext/zip</uri></json:item><istex:fulltextTEI uri="https://api.istex.fr/document/55329048E08E1F6C15C86FA78D0F4652CEDF10FD/fulltext/tei"><teiHeader><fileDesc><titleStmt><title level="a" type="main" xml:lang="en">Neuropeptide regulation of feeding in catfish, Ictalurus punctatus : a role for glucagon-like peptide-1 (GLP-1)?</title></titleStmt><publicationStmt><authority>ISTEX</authority><publisher>ELSEVIER</publisher><availability><p>©2001 Elsevier Science Inc.</p></availability><date>2001</date></publicationStmt><notesStmt><note type="content">Fig. 1: Dose response in feed intake (% BW) to ICV injected cfGLP-1. Sample size was n=8 for control and n=4 for each dose. Points with different superscripts indicate significant difference at P<0.01. The dose at which intake was 50% of controls is indicated by the arrow.</note><note type="content">Fig. 2: (a) Feed intake following i.v. injection of cfGLP-1, 150 ng g−1 BW. The asterisk indicates a significant difference (P<0.04) between cfGLP-1 injected Norris fish compared with their PBS injected counterparts, the difference in USDA-103 fish was not significant (n=8). (b) Intraperitoneal injection of fish from the Norris strain with PBS or 100 ng g−1 BW cfGLP-1 (n=6).</note><note type="content">Fig. 3: (a) Feed consumption following ICV injection of PBS (control), cfGLP-1 (0.3 ng g−1 BW), the putative GLP-1 receptor antagonist exendin9–39 (12.5 ng g−1 BW), and cfGLP-1+exendin9–39 (same doses as above). All treatments (n=14) were administered in 2 μl volume. Feed consumption was normalized to controls. Bars with different superscripts are significantly different (P<0.005). (b) Time course of feed consumption (normalized to control initial intake value) after ICV injection of hGLP-1 (30 ng g−1 BW), PBS and undiluted antisera against salmon GLP-1 (GLPab). All injections were in 2 μl volume. At times 1 and 3 h: n=4, and at 8, 20 and 48 h: n=8. Bars with different superscripts within a time point are significantly different (P<0.05).</note></notesStmt><sourceDesc><biblStruct type="inbook"><analytic><title level="a" type="main" xml:lang="en">Neuropeptide regulation of feeding in catfish, Ictalurus punctatus : a role for glucagon-like peptide-1 (GLP-1)?</title><author xml:id="author-1"><persName><forename type="first">Jeffrey T.</forename><surname>Silverstein</surname></persName><email>jsilvers@ars.usda.gov</email><note type="correspondence"><p>Corresponding author. Tel.: +1-662-686-3591; fax: +1-662-686-3567</p></note><affiliation>USDA, ARS, Catfish Genetics Research Unit, Thad Cochran National Warmwater Aquaculture Center, Stoneville, MS, 38776, USA</affiliation></author><author xml:id="author-2"><persName><forename type="first">Vera M.</forename><surname>Bondareva</surname></persName><affiliation>Sechenov Institute of Evolutionary Physiology and Biochemistry, St. Petersburg, Russia</affiliation></author><author xml:id="author-3"><persName><forename type="first">Jill B.K.</forename><surname>Leonard</surname></persName><note type="biography">Current address: Biology Department, Northern Michigan University, Marquette, MI 49855, USA.</note><affiliation>Current address: Biology Department, Northern Michigan University, Marquette, MI 49855, USA.</affiliation><affiliation>USDA, ARS, Catfish Genetics Research Unit, Thad Cochran National Warmwater Aquaculture Center, Stoneville, MS, 38776, USA</affiliation></author><author xml:id="author-4"><persName><forename type="first">Erika M.</forename><surname>Plisetskaya</surname></persName><affiliation>University of Washington, School of Fisheries, Seattle, WA 98195 USA</affiliation></author></analytic><monogr><title level="j">Comparative Biochemistry and Physiology, Part B: Biochemistry and Molecular Biology</title><title level="j" type="abbrev">CBB</title><idno type="pISSN">1096-4959</idno><idno type="PII">S1096-4959(00)X0006-1</idno><imprint><publisher>ELSEVIER</publisher><date type="published" when="2001"></date><biblScope unit="volume">129</biblScope><biblScope unit="issue">2–3</biblScope><biblScope unit="page" from="623">623</biblScope><biblScope unit="page" to="631">631</biblScope></imprint></monogr><idno type="istex">55329048E08E1F6C15C86FA78D0F4652CEDF10FD</idno><idno type="DOI">10.1016/S1096-4959(01)00357-8</idno><idno type="PII">S1096-4959(01)00357-8</idno></biblStruct></sourceDesc></fileDesc><profileDesc><creation><date>2001</date></creation><langUsage><language ident="en">en</language></langUsage><abstract xml:lang="en"><p>Glucagon-like peptide 1 is a compound known to cause reduced food intake in mammals, though its action on feed intake in fish is unknown. The clear differences in the effects of GLP-1 on mammalian and teleostean glucose homeostasis suggest that we cannot assume a similar action of GLP-1 on feeding in mammals and fish. In this study the effects and specificity of centrally administered GLP-1 on feed intake were examined. It was demonstrated that intracerebroventricular (ICV) injection of glucagon-like peptide 1 (GLP-1) in the channel catfish (Ictalurus punctatus) is a potent inhibitor of feed intake with a dose of 0.25 ng g−1 body wt. reducing feed intake by 50%. The weak response to intraperitoneal (i.p.) and intravenous (i.v.) injection treatments with GLP-1 suggests the major effects on feed intake are centrally mediated. GLP-1 action on feed intake was not antagonized by ICV injection of exendin9–39. Immunoneutralization of GLP-1 by ICV injection of antisalmon GLP-1 antisera did not affect feed intake over 48 h, while ICV injection of GLP-1 at a dose of 30 ng g−1 body wt. reduced feed intake for over 20 h. Additionally, there is some evidence that GLP-1 caused gastric evacuation. We conclude that GLP-1 is a potent inhibitor of feeding in fish, but its involvement in feed intake regulation under physiological conditions remains to be clarified.</p></abstract><textClass xml:lang="en"><keywords scheme="keyword"><list><head>Keywords</head><item><term>Feeding</term></item><item><term>Feed intake regulation</term></item><item><term>Glucagon-like peptide-1</term></item><item><term>Intracerebroventricular injection</term></item><item><term>Exendin9–39</term></item><item><term>Immunoneutralization</term></item><item><term>Catfish</term></item><item><term>Ictalurus punctatus</term></item></list></keywords></textClass></profileDesc><revisionDesc><change when="2001-01-05">Modified</change><change when="2001">Published</change></revisionDesc></teiHeader></istex:fulltextTEI><json:item><extension>txt</extension><original>false</original><mimetype>text/plain</mimetype><uri>https://api.istex.fr/document/55329048E08E1F6C15C86FA78D0F4652CEDF10FD/fulltext/txt</uri></json:item></fulltext><metadata><istex:metadataXml wicri:clean="Elsevier, elements deleted: ce:floats; body; tail"><istex:xmlDeclaration>version="1.0" encoding="utf-8"</istex:xmlDeclaration><istex:docType PUBLIC="-//ES//DTD journal article DTD version 4.5.2//EN//XML" URI="art452.dtd" name="istex:docType"><istex:entity SYSTEM="gr1" NDATA="IMAGE" name="gr1"></istex:entity><istex:entity SYSTEM="gr2" NDATA="IMAGE" name="gr2"></istex:entity><istex:entity SYSTEM="gr3" NDATA="IMAGE" name="gr3"></istex:entity></istex:docType><istex:document><converted-article version="4.5.2" docsubtype="fla" xml:lang="en"><item-info><jid>CBB</jid><aid>7611</aid><ce:pii>S1096-4959(01)00357-8</ce:pii><ce:doi>10.1016/S1096-4959(01)00357-8</ce:doi><ce:copyright type="full-transfer" year="2001">Elsevier Science Inc.</ce:copyright></item-info><head><ce:title>Neuropeptide regulation of feeding in catfish, <ce:italic>Ictalurus punctatus</ce:italic>: a role for glucagon-like peptide-1 (GLP-1)?</ce:title><ce:author-group><ce:author><ce:given-name>Jeffrey T.</ce:given-name><ce:surname>Silverstein</ce:surname><ce:cross-ref refid="COR1">*</ce:cross-ref><ce:cross-ref refid="AFF1"><ce:sup>a</ce:sup></ce:cross-ref><ce:e-address type="email">jsilvers@ars.usda.gov</ce:e-address></ce:author><ce:author><ce:given-name>Vera M.</ce:given-name><ce:surname>Bondareva</ce:surname><ce:cross-ref refid="AFF2"><ce:sup>b</ce:sup></ce:cross-ref></ce:author><ce:author><ce:given-name>Jill B.K.</ce:given-name><ce:surname>Leonard</ce:surname><ce:cross-ref refid="AFF1"><ce:sup>a</ce:sup></ce:cross-ref><ce:cross-ref refid="AFF3"><ce:sup>c</ce:sup></ce:cross-ref><ce:cross-ref refid="FN1"><ce:sup>1</ce:sup></ce:cross-ref></ce:author><ce:author><ce:given-name>Erika M.</ce:given-name><ce:surname>Plisetskaya</ce:surname><ce:cross-ref refid="AFF3"><ce:sup>c</ce:sup></ce:cross-ref></ce:author><ce:affiliation id="AFF1"><ce:label>a</ce:label><ce:textfn>USDA, ARS, Catfish Genetics Research Unit, Thad Cochran National Warmwater Aquaculture Center, Stoneville, MS, 38776, USA</ce:textfn></ce:affiliation><ce:affiliation id="AFF2"><ce:label>b</ce:label><ce:textfn>Sechenov Institute of Evolutionary Physiology and Biochemistry, St. Petersburg, Russia</ce:textfn></ce:affiliation><ce:affiliation id="AFF3"><ce:label>c</ce:label><ce:textfn>University of Washington, School of Fisheries, Seattle, WA 98195 USA</ce:textfn></ce:affiliation><ce:correspondence id="COR1"><ce:label>*</ce:label><ce:text>Corresponding author. Tel.: +1-662-686-3591; fax: +1-662-686-3567</ce:text></ce:correspondence><ce:footnote id="FN1"><ce:label>1</ce:label><ce:note-para>Current address: Biology Department, Northern Michigan University, Marquette, MI 49855, USA.</ce:note-para></ce:footnote></ce:author-group><ce:date-received day="10" month="10" year="2000"></ce:date-received><ce:date-revised day="5" month="1" year="2001"></ce:date-revised><ce:date-accepted day="15" month="1" year="2001"></ce:date-accepted><ce:abstract><ce:section-title>Abstract</ce:section-title><ce:abstract-sec><ce:simple-para>Glucagon-like peptide 1 is a compound known to cause reduced food intake in mammals, though its action on feed intake in fish is unknown. The clear differences in the effects of GLP-1 on mammalian and teleostean glucose homeostasis suggest that we cannot assume a similar action of GLP-1 on feeding in mammals and fish. In this study the effects and specificity of centrally administered GLP-1 on feed intake were examined. It was demonstrated that intracerebroventricular (ICV) injection of glucagon-like peptide 1 (GLP-1) in the channel catfish (<ce:italic>Ictalurus punctatus</ce:italic>) is a potent inhibitor of feed intake with a dose of 0.25 ng g<ce:sup>−1</ce:sup> body wt. reducing feed intake by 50%. The weak response to intraperitoneal (i.p.) and intravenous (i.v.) injection treatments with GLP-1 suggests the major effects on feed intake are centrally mediated. GLP-1 action on feed intake was not antagonized by ICV injection of exendin<ce:inf>9–39</ce:inf>. Immunoneutralization of GLP-1 by ICV injection of antisalmon GLP-1 antisera did not affect feed intake over 48 h, while ICV injection of GLP-1 at a dose of 30 ng g<ce:sup>−1</ce:sup> body wt. reduced feed intake for over 20 h. Additionally, there is some evidence that GLP-1 caused gastric evacuation. We conclude that GLP-1 is a potent inhibitor of feeding in fish, but its involvement in feed intake regulation under physiological conditions remains to be clarified.</ce:simple-para></ce:abstract-sec></ce:abstract><ce:keywords class="keyword"><ce:section-title>Keywords</ce:section-title><ce:keyword><ce:text>Feeding</ce:text></ce:keyword><ce:keyword><ce:text>Feed intake regulation</ce:text></ce:keyword><ce:keyword><ce:text>Glucagon-like peptide-1</ce:text></ce:keyword><ce:keyword><ce:text>Intracerebroventricular injection</ce:text></ce:keyword><ce:keyword><ce:text>Exendin<ce:inf>9–39</ce:inf></ce:text></ce:keyword><ce:keyword><ce:text>Immunoneutralization</ce:text></ce:keyword><ce:keyword><ce:text>Catfish</ce:text></ce:keyword><ce:keyword><ce:text><ce:italic>Ictalurus punctatus</ce:italic></ce:text></ce:keyword></ce:keywords></head></converted-article></istex:document></istex:metadataXml><mods version="3.6"><titleInfo lang="en"><title>Neuropeptide regulation of feeding in catfish, Ictalurus punctatus : a role for glucagon-like peptide-1 (GLP-1)?</title></titleInfo><titleInfo type="alternative" lang="en" contentType="CDATA"><title>Neuropeptide regulation of feeding in catfish,</title></titleInfo><name type="personal"><namePart type="given">Jeffrey T.</namePart><namePart type="family">Silverstein</namePart><affiliation>E-mail: jsilvers@ars.usda.gov</affiliation><affiliation>USDA, ARS, Catfish Genetics Research Unit, Thad Cochran National Warmwater Aquaculture Center, Stoneville, MS, 38776, USA</affiliation><description>Corresponding author. Tel.: +1-662-686-3591; fax: +1-662-686-3567</description><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">Vera M.</namePart><namePart type="family">Bondareva</namePart><affiliation>Sechenov Institute of Evolutionary Physiology and Biochemistry, St. Petersburg, Russia</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">Jill B.K.</namePart><namePart type="family">Leonard</namePart><affiliation>USDA, ARS, Catfish Genetics Research Unit, Thad Cochran National Warmwater Aquaculture Center, Stoneville, MS, 38776, USA</affiliation><description>Current address: Biology Department, Northern Michigan University, Marquette, MI 49855, USA.</description><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">Erika M.</namePart><namePart type="family">Plisetskaya</namePart><affiliation>University of Washington, School of Fisheries, Seattle, WA 98195 USA</affiliation><role><roleTerm type="text">author</roleTerm></role></name><typeOfResource>text</typeOfResource><genre type="research-article" displayLabel="Full-length article"></genre><originInfo><publisher>ELSEVIER</publisher><dateIssued encoding="w3cdtf">2001</dateIssued><dateModified encoding="w3cdtf">2001-01-05</dateModified><copyrightDate encoding="w3cdtf">2001</copyrightDate></originInfo><language><languageTerm type="code" authority="iso639-2b">eng</languageTerm><languageTerm type="code" authority="rfc3066">en</languageTerm></language><physicalDescription><internetMediaType>text/html</internetMediaType></physicalDescription><abstract lang="en">Glucagon-like peptide 1 is a compound known to cause reduced food intake in mammals, though its action on feed intake in fish is unknown. The clear differences in the effects of GLP-1 on mammalian and teleostean glucose homeostasis suggest that we cannot assume a similar action of GLP-1 on feeding in mammals and fish. In this study the effects and specificity of centrally administered GLP-1 on feed intake were examined. It was demonstrated that intracerebroventricular (ICV) injection of glucagon-like peptide 1 (GLP-1) in the channel catfish (Ictalurus punctatus) is a potent inhibitor of feed intake with a dose of 0.25 ng g−1 body wt. reducing feed intake by 50%. The weak response to intraperitoneal (i.p.) and intravenous (i.v.) injection treatments with GLP-1 suggests the major effects on feed intake are centrally mediated. GLP-1 action on feed intake was not antagonized by ICV injection of exendin9–39. Immunoneutralization of GLP-1 by ICV injection of antisalmon GLP-1 antisera did not affect feed intake over 48 h, while ICV injection of GLP-1 at a dose of 30 ng g−1 body wt. reduced feed intake for over 20 h. Additionally, there is some evidence that GLP-1 caused gastric evacuation. We conclude that GLP-1 is a potent inhibitor of feeding in fish, but its involvement in feed intake regulation under physiological conditions remains to be clarified.</abstract><note type="content">Fig. 1: Dose response in feed intake (% BW) to ICV injected cfGLP-1. Sample size was n=8 for control and n=4 for each dose. Points with different superscripts indicate significant difference at P<0.01. The dose at which intake was 50% of controls is indicated by the arrow.</note><note type="content">Fig. 2: (a) Feed intake following i.v. injection of cfGLP-1, 150 ng g−1 BW. The asterisk indicates a significant difference (P<0.04) between cfGLP-1 injected Norris fish compared with their PBS injected counterparts, the difference in USDA-103 fish was not significant (n=8). (b) Intraperitoneal injection of fish from the Norris strain with PBS or 100 ng g−1 BW cfGLP-1 (n=6).</note><note type="content">Fig. 3: (a) Feed consumption following ICV injection of PBS (control), cfGLP-1 (0.3 ng g−1 BW), the putative GLP-1 receptor antagonist exendin9–39 (12.5 ng g−1 BW), and cfGLP-1+exendin9–39 (same doses as above). All treatments (n=14) were administered in 2 μl volume. Feed consumption was normalized to controls. Bars with different superscripts are significantly different (P<0.005). (b) Time course of feed consumption (normalized to control initial intake value) after ICV injection of hGLP-1 (30 ng g−1 BW), PBS and undiluted antisera against salmon GLP-1 (GLPab). All injections were in 2 μl volume. At times 1 and 3 h: n=4, and at 8, 20 and 48 h: n=8. Bars with different superscripts within a time point are significantly different (P<0.05).</note><subject lang="en"><genre>Keywords</genre><topic>Feeding</topic><topic>Feed intake regulation</topic><topic>Glucagon-like peptide-1</topic><topic>Intracerebroventricular injection</topic><topic>Exendin9–39</topic><topic>Immunoneutralization</topic><topic>Catfish</topic><topic>Ictalurus punctatus</topic></subject><relatedItem type="host"><titleInfo><title>Comparative Biochemistry and Physiology, Part B: Biochemistry and Molecular Biology</title></titleInfo><titleInfo type="abbreviated"><title>CBB</title></titleInfo><genre type="journal">journal</genre><originInfo><dateIssued encoding="w3cdtf">200106</dateIssued></originInfo><identifier type="ISSN">1096-4959</identifier><identifier type="PII">S1096-4959(00)X0006-1</identifier><part><date>200106</date><detail type="issue"><title>4TH International Symposium on Fish Endocrinology</title></detail><detail type="volume"><number>129</number><caption>vol.</caption></detail><detail type="issue"><number>2–3</number><caption>no.</caption></detail><extent unit="issue pages"><start>205</start><end>702</end></extent><extent unit="pages"><start>623</start><end>631</end></extent></part></relatedItem><identifier type="istex">55329048E08E1F6C15C86FA78D0F4652CEDF10FD</identifier><identifier type="DOI">10.1016/S1096-4959(01)00357-8</identifier><identifier type="PII">S1096-4959(01)00357-8</identifier><accessCondition type="use and reproduction" contentType="copyright">©2001 Elsevier Science Inc.</accessCondition><recordInfo><recordContentSource>ELSEVIER</recordContentSource><recordOrigin>Elsevier Science Inc., ©2001</recordOrigin></recordInfo></mods></metadata><serie></serie></istex></record>Pour manipuler ce document sous Unix (Dilib)
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