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Immunocytochemical demonstration of somatotropin-like and prolactin-like activity in the brain of Calamoichthys calabaricus (Actinopterygii)

Identifieur interne : 000D83 ( Istex/Corpus ); précédent : 000D82; suivant : 000D84

Immunocytochemical demonstration of somatotropin-like and prolactin-like activity in the brain of Calamoichthys calabaricus (Actinopterygii)

Auteurs : Bente Langvad Hansen ; Georg N Rgaard Hansen

Source :

RBID : ISTEX:310E291855560EED7C2983B2857B4CF0341D05E6

Abstract

Summary: Cellular binding of anti-bSTH and anti-oPRL IgG is demonstrated in the brain and the pituitary gland of the African freshwater fish Calamoichthys calabaricus by means of the unlabeled antibody enzyme method at the light microscopic level. In the brain, somatotropin and prolactin are demonstrated in separate neurons in the preoptic area. The somatotropinergic and prolactinergic perikarya are distinct from those of the hypothalamic-hypophysial neurosecretory neurons, i.e., those stainable with aldehyde fuchsin presumed to be vasotocinergic and isotocinergic. The somatotropinergic and prolactinergic neuronal perikarya give rise to separate beaded axons which pass either ventroposteriorly into the infundibulum, terminating in the neurohypophysis, or ventro-laterally through the wall of the preoptic recess, terminating near the superficial capillary bed covering this part of the brain surface. Moreover, coarse dendrite-like processes of both kinds of immuno-reactive neurons extend towards, and end in, the third ventricle. Binding sites in the brain to antisera against hLHβ, hFSHβ, hTSHβ and anti-(1–24) ACTH IgG, all reactive in the pituitary, are not observed in the neurons confined to the preoptic area.

Url:
DOI: 10.1007/BF00213859

Links to Exploration step

ISTEX:310E291855560EED7C2983B2857B4CF0341D05E6

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<Keyword>Nucleus supraopticus</Keyword>
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<abstract lang="en">Summary: Cellular binding of anti-bSTH and anti-oPRL IgG is demonstrated in the brain and the pituitary gland of the African freshwater fish Calamoichthys calabaricus by means of the unlabeled antibody enzyme method at the light microscopic level. In the brain, somatotropin and prolactin are demonstrated in separate neurons in the preoptic area. The somatotropinergic and prolactinergic perikarya are distinct from those of the hypothalamic-hypophysial neurosecretory neurons, i.e., those stainable with aldehyde fuchsin presumed to be vasotocinergic and isotocinergic. The somatotropinergic and prolactinergic neuronal perikarya give rise to separate beaded axons which pass either ventroposteriorly into the infundibulum, terminating in the neurohypophysis, or ventro-laterally through the wall of the preoptic recess, terminating near the superficial capillary bed covering this part of the brain surface. Moreover, coarse dendrite-like processes of both kinds of immuno-reactive neurons extend towards, and end in, the third ventricle. Binding sites in the brain to antisera against hLHβ, hFSHβ, hTSHβ and anti-(1–24) ACTH IgG, all reactive in the pituitary, are not observed in the neurons confined to the preoptic area.</abstract>
<relatedItem type="host">
<titleInfo>
<title>Cell and Tissue Research</title>
</titleInfo>
<titleInfo type="abbreviated">
<title>Cell Tissue Res.</title>
</titleInfo>
<genre type="journal" displayLabel="Archive Journal"></genre>
<originInfo>
<dateIssued encoding="w3cdtf">1982-02-01</dateIssued>
<copyrightDate encoding="w3cdtf">1982</copyrightDate>
</originInfo>
<subject>
<genre>Biomedicine</genre>
<topic>Neurosciences</topic>
<topic>Endocrinology</topic>
<topic>Neurology</topic>
<topic>Cell Biology</topic>
</subject>
<identifier type="ISSN">0302-766X</identifier>
<identifier type="eISSN">1432-0878</identifier>
<identifier type="JournalID">441</identifier>
<identifier type="IssueArticleCount">21</identifier>
<identifier type="VolumeIssueCount">3</identifier>
<part>
<date>1982</date>
<detail type="volume">
<number>222</number>
<caption>vol.</caption>
</detail>
<detail type="issue">
<number>3</number>
<caption>no.</caption>
</detail>
<extent unit="pages">
<start>615</start>
<end>627</end>
</extent>
</part>
<recordInfo>
<recordOrigin>Springer-Verlag GmbH & Co. KG, 1982</recordOrigin>
</recordInfo>
</relatedItem>
<identifier type="istex">310E291855560EED7C2983B2857B4CF0341D05E6</identifier>
<identifier type="DOI">10.1007/BF00213859</identifier>
<identifier type="ArticleID">BF00213859</identifier>
<identifier type="ArticleID">Art12</identifier>
<accessCondition type="use and reproduction" contentType="copyright">Springer-Verlag, 1982</accessCondition>
<recordInfo>
<recordContentSource>SPRINGER</recordContentSource>
<recordOrigin>Springer-Verlag, 1982</recordOrigin>
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   |texte=   Immunocytochemical demonstration of somatotropin-like and prolactin-like activity in the brain of Calamoichthys calabaricus (Actinopterygii)
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