Pressure and cold shock induction of meiotic gynogenesis and triploidy in the European sea bass, Dicentrarchus labrax L.: relative efficiency of methods and parental variability
Identifieur interne : 000C63 ( Istex/Corpus ); précédent : 000C62; suivant : 000C64Pressure and cold shock induction of meiotic gynogenesis and triploidy in the European sea bass, Dicentrarchus labrax L.: relative efficiency of methods and parental variability
Auteurs : Stefano Peruzzi ; Beatrice ChatainSource :
- Aquaculture [ 0044-8486 ] ; 2000.
Abstract
The optimal conditions for the retention of the second polar body in sea bass eggs were investigated by altering the timing, intensity and duration of application of pressure and cold shocks. Treatment optima for cold shocks were 0–1°C for 15–20 min at 5 min after fertilisation (a.f.) and 8500 psi for 2 min at 6 min a.f. for pressure shocks. Meiogenesis was obtained by fertilising eggs with UV-irradiated homologous sperm (32,000 erg mm−2) and pressure or cold shocking eggs as above. 100% triploidy was induced following definition of liable periods for the disruption of the meiotic spindle obtained in gynogenesis. Ploidy investigations were performed on experimental groups by flow-cytometry. Verification of uniparental transmission in meiogens was carried out by microsatellite marker loci analysis. This work highlights the degree of variation in individual responses of selected broodstock to these agents. Finally, some preliminary results on heterologous fertilisation in sea bass with potential applications for gynogenetic studies are also provided.
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DOI: 10.1016/S0044-8486(00)00355-0
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Tel.: +33-4675-041-09; fax: +33-4676-828-85</mods:affiliation></affiliation><affiliation><mods:affiliation>E-mail: bchatain@ifremer.fr</mods:affiliation></affiliation></author></titleStmt><publicationStmt><idno type="wicri:source">ISTEX</idno><idno type="RBID">ISTEX:137D45329C8625CEAF8D45F18F986669F4B64709</idno><date when="2000" year="2000">2000</date><idno type="doi">10.1016/S0044-8486(00)00355-0</idno><idno type="url">https://api.istex.fr/document/137D45329C8625CEAF8D45F18F986669F4B64709/fulltext/pdf</idno><idno type="wicri:Area/Istex/Corpus">000C63</idno><idno type="wicri:explorRef" wicri:stream="Istex" wicri:step="Corpus" wicri:corpus="ISTEX">000C63</idno></publicationStmt><sourceDesc><biblStruct><analytic><title level="a">Pressure and cold shock induction of meiotic gynogenesis and triploidy in the European sea bass, Dicentrarchus labrax L.: relative efficiency of methods and parental variability</title><author><name sortKey="Peruzzi, Stefano" sort="Peruzzi, Stefano" uniqKey="Peruzzi S" first="Stefano" last="Peruzzi">Stefano Peruzzi</name><affiliation><mods:affiliation>CEFREM, Centre de Formation et de Recherche sur l'Environnement Marin, CNRS UMR 5110, Université de Perpignan, 52 avenue de Villeneuve, 66860 Perpignan, France</mods:affiliation></affiliation></author><author><name sortKey="Chatain, Beatrice" sort="Chatain, Beatrice" uniqKey="Chatain B" first="Beatrice" last="Chatain">Beatrice Chatain</name><affiliation><mods:affiliation>Station Expérimentale d'Acquaculture IFREMER, chemin de Maguelone, 34250 Palavas-les-Flots, France</mods:affiliation></affiliation><affiliation><mods:affiliation>Corresponding author. Tel.: +33-4675-041-09; fax: +33-4676-828-85</mods:affiliation></affiliation><affiliation><mods:affiliation>E-mail: bchatain@ifremer.fr</mods:affiliation></affiliation></author></analytic><monogr></monogr><series><title level="j">Aquaculture</title><title level="j" type="abbrev">AQUA</title><idno type="ISSN">0044-8486</idno><imprint><publisher>ELSEVIER</publisher><date type="published" when="2000">2000</date><biblScope unit="volume">189</biblScope><biblScope unit="issue">1–2</biblScope><biblScope unit="page" from="23">23</biblScope><biblScope unit="page" to="37">37</biblScope></imprint><idno type="ISSN">0044-8486</idno></series><idno type="istex">137D45329C8625CEAF8D45F18F986669F4B64709</idno><idno type="DOI">10.1016/S0044-8486(00)00355-0</idno><idno type="PII">S0044-8486(00)00355-0</idno></biblStruct></sourceDesc><seriesStmt><idno type="ISSN">0044-8486</idno></seriesStmt></fileDesc><profileDesc><textClass></textClass><langUsage><language ident="en">en</language></langUsage></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">The optimal conditions for the retention of the second polar body in sea bass eggs were investigated by altering the timing, intensity and duration of application of pressure and cold shocks. Treatment optima for cold shocks were 0–1°C for 15–20 min at 5 min after fertilisation (a.f.) and 8500 psi for 2 min at 6 min a.f. for pressure shocks. Meiogenesis was obtained by fertilising eggs with UV-irradiated homologous sperm (32,000 erg mm−2) and pressure or cold shocking eggs as above. 100% triploidy was induced following definition of liable periods for the disruption of the meiotic spindle obtained in gynogenesis. Ploidy investigations were performed on experimental groups by flow-cytometry. Verification of uniparental transmission in meiogens was carried out by microsatellite marker loci analysis. This work highlights the degree of variation in individual responses of selected broodstock to these agents. Finally, some preliminary results on heterologous fertilisation in sea bass with potential applications for gynogenetic studies are also provided.</div></front></TEI><istex><corpusName>elsevier</corpusName><author><json:item><name>Stefano Peruzzi</name><affiliations><json:string>CEFREM, Centre de Formation et de Recherche sur l'Environnement Marin, CNRS UMR 5110, Université de Perpignan, 52 avenue de Villeneuve, 66860 Perpignan, France</json:string></affiliations></json:item><json:item><name>Beatrice Chatain</name><affiliations><json:string>Station Expérimentale d'Acquaculture IFREMER, chemin de Maguelone, 34250 Palavas-les-Flots, France</json:string><json:string>Corresponding author. Tel.: +33-4675-041-09; fax: +33-4676-828-85</json:string><json:string>E-mail: bchatain@ifremer.fr</json:string></affiliations></json:item></author><subject><json:item><lang><json:string>eng</json:string></lang><value>Meiotic gynogenesis</value></json:item><json:item><lang><json:string>eng</json:string></lang><value>Triploidy</value></json:item><json:item><lang><json:string>eng</json:string></lang><value>Sea bass</value></json:item><json:item><lang><json:string>eng</json:string></lang><value>Dicentrarchus labrax</value></json:item><json:item><lang><json:string>eng</json:string></lang><value>Pressure and cold shocks</value></json:item><json:item><lang><json:string>eng</json:string></lang><value>Parental variability</value></json:item></subject><language><json:string>eng</json:string></language><originalGenre><json:string>Full-length article</json:string></originalGenre><abstract>The optimal conditions for the retention of the second polar body in sea bass eggs were investigated by altering the timing, intensity and duration of application of pressure and cold shocks. Treatment optima for cold shocks were 0–1°C for 15–20 min at 5 min after fertilisation (a.f.) and 8500 psi for 2 min at 6 min a.f. for pressure shocks. Meiogenesis was obtained by fertilising eggs with UV-irradiated homologous sperm (32,000 erg mm−2) and pressure or cold shocking eggs as above. 100% triploidy was induced following definition of liable periods for the disruption of the meiotic spindle obtained in gynogenesis. Ploidy investigations were performed on experimental groups by flow-cytometry. Verification of uniparental transmission in meiogens was carried out by microsatellite marker loci analysis. This work highlights the degree of variation in individual responses of selected broodstock to these agents. Finally, some preliminary results on heterologous fertilisation in sea bass with potential applications for gynogenetic studies are also provided.</abstract><qualityIndicators><score>6.896</score><pdfVersion>1.2</pdfVersion><pdfPageSize>435 x 643 pts</pdfPageSize><refBibsNative>true</refBibsNative><keywordCount>6</keywordCount><abstractCharCount>1064</abstractCharCount><pdfWordCount>5602</pdfWordCount><pdfCharCount>33516</pdfCharCount><pdfPageCount>15</pdfPageCount><abstractWordCount>158</abstractWordCount></qualityIndicators><title>Pressure and cold shock induction of meiotic gynogenesis and triploidy in the European sea bass, Dicentrarchus labrax L.: relative efficiency of methods and parental variability</title><pii><json:string>S0044-8486(00)00355-0</json:string></pii><genre><json:string>research-article</json:string></genre><serie><pages><last>143</last><first>125</first></pages><language><json:string>unknown</json:string></language><title>OECD Documents, Environmental Impacts of Aquatic Biotechnology</title></serie><host><volume>189</volume><pii><json:string>S0044-8486(00)X0113-5</json:string></pii><pages><last>37</last><first>23</first></pages><issn><json:string>0044-8486</json:string></issn><issue>1–2</issue><genre><json:string>journal</json:string></genre><language><json:string>unknown</json:string></language><title>Aquaculture</title><publicationDate>2000</publicationDate></host><categories><wos><json:string>science</json:string><json:string>marine & freshwater biology</json:string><json:string>fisheries</json:string></wos><scienceMetrix><json:string>applied sciences</json:string><json:string>agriculture, fisheries & forestry</json:string><json:string>fisheries</json:string></scienceMetrix></categories><publicationDate>2000</publicationDate><copyrightDate>2000</copyrightDate><doi><json:string>10.1016/S0044-8486(00)00355-0</json:string></doi><id>137D45329C8625CEAF8D45F18F986669F4B64709</id><score>0.03582628</score><fulltext><json:item><extension>pdf</extension><original>true</original><mimetype>application/pdf</mimetype><uri>https://api.istex.fr/document/137D45329C8625CEAF8D45F18F986669F4B64709/fulltext/pdf</uri></json:item><json:item><extension>zip</extension><original>false</original><mimetype>application/zip</mimetype><uri>https://api.istex.fr/document/137D45329C8625CEAF8D45F18F986669F4B64709/fulltext/zip</uri></json:item><istex:fulltextTEI uri="https://api.istex.fr/document/137D45329C8625CEAF8D45F18F986669F4B64709/fulltext/tei"><teiHeader><fileDesc><titleStmt><title level="a">Pressure and cold shock induction of meiotic gynogenesis and triploidy in the European sea bass, Dicentrarchus labrax L.: relative efficiency of methods and parental variability</title></titleStmt><publicationStmt><authority>ISTEX</authority><publisher>ELSEVIER</publisher><availability><p>©2000 Elsevier Science B.V.</p></availability><date>2000</date></publicationStmt><notesStmt><note type="content">Fig. 1: Survival of D. labrax eggs fertilised with UV irradiated sperm. Means with a common superscript are not significantly different by χ2 test (P<0.05). Results observed at 4 h after fertilisation. (a) Mean and standard error of survival rates for the four tested couples. 2n: diploid status; n: haploid status. (b) Survival rate in each couple relative to control. At this stage, survival rates in control groups were of 90%, 83%, 98% and 90% for couples 1, 2, 3 and 4, respectively. Results observed at 72 h after fertilisation. (c) Mean and standard error of survival rate for the four tested couples. 2n: diploid status; n: haploid status. (d) Survival rate in each couple relative to control. At this stage, survival rates in control groups were of 56%, 70%, 69% and 53% for couples 1, 2, 3 and 4, respectively.</note><note type="content">Fig. 2: D. labrax haploid, diploid and triploid larvae and their respective nuclear DNA content measured by flow cytometry (M1, M2, M3). DNA values are reported in arbitrary units expressed as fluorescence channel numbers (FL2-Area). Scale bars represent 1 mm.</note><note type="content">Fig. 3: Survival of D. labrax eggs activated with homologous and heterologous sperm, from fertilisation to hatching. Spawning were issued from two different females (female 1: –; female 2: ---). • D. labrax sperm. ○ D. labrax irradiated sperm (total UV dose of 32.000 erg mm−2). ▴ Sparus aurata sperm.</note><note type="content">Fig. 4: Survival of D. labrax eggs fertilised with UV irradiated sperm (total UV dose of 32.000 erg mm−2). Eggs were submitted to a pressure shock (▩, 8000 psi for 2 min) or to a cold shock (□, 1°C for 20 min) applied at different moments after fertilisation. Results represent survival rates observed 72 h after fertilisation. Means with a common superscript are not significantly different by χ2 test (P<0.05). (a) Mean and standard error of survival rates for the four tested couples. 2n: diploid status; n:haploid status; ▨, diploid control groups. (b) Survival rate in each couple relative to control. At this stage, survival rates in control groups were of 69%, 66%, 97% and 70% for couples 1, 2, 3 and 4, respectively.</note><note type="content">Fig. 5: Observed Labrax 17 DNA banding pattern in control and gynogenetic progenies of D. labrax. Meiotic gynogens homozygous for one of the maternal alleles are indicated by arrows. F: female parent; M: male parent; bp: allele sizes given in base pairs.</note><note type="content">Fig. 6: Survival of D. labrax eggs fertilised with non-irradiated sperm. Eggs were submitted to pressure or cold shocks of different levels. Pressure shocks (▩) were applied 6 min after fertilisation for 2 min. Cold shocks of 1°C (□) were applied 5 min after fertilisation. Results represent the survival rates observed 72 h after fertilisation. Means with a common superscript are not significantly different by χ2 test (P<0.05). (a) Mean and standard error of survival rates for the three tested couples. 2n: diploid status; 3n: triploid status; ▨, diploid control groups. (b) Survival rates in each couple relative to control. At this stage, survival rates in control groups were of 76%, 95% and 88% for couples 1, 2, 3 and 4, respectively.</note></notesStmt><sourceDesc><biblStruct type="inbook"><analytic><title level="a">Pressure and cold shock induction of meiotic gynogenesis and triploidy in the European sea bass, Dicentrarchus labrax L.: relative efficiency of methods and parental variability</title><author xml:id="author-1"><persName><forename type="first">Stefano</forename><surname>Peruzzi</surname></persName><affiliation>CEFREM, Centre de Formation et de Recherche sur l'Environnement Marin, CNRS UMR 5110, Université de Perpignan, 52 avenue de Villeneuve, 66860 Perpignan, France</affiliation></author><author xml:id="author-2"><persName><forename type="first">Beatrice</forename><surname>Chatain</surname></persName><email>bchatain@ifremer.fr</email><affiliation>Station Expérimentale d'Acquaculture IFREMER, chemin de Maguelone, 34250 Palavas-les-Flots, France</affiliation><affiliation>Corresponding author. Tel.: +33-4675-041-09; fax: +33-4676-828-85</affiliation></author></analytic><monogr><title level="j">Aquaculture</title><title level="j" type="abbrev">AQUA</title><idno type="pISSN">0044-8486</idno><idno type="PII">S0044-8486(00)X0113-5</idno><imprint><publisher>ELSEVIER</publisher><date type="published" when="2000"></date><biblScope unit="volume">189</biblScope><biblScope unit="issue">1–2</biblScope><biblScope unit="page" from="23">23</biblScope><biblScope unit="page" to="37">37</biblScope></imprint></monogr><idno type="istex">137D45329C8625CEAF8D45F18F986669F4B64709</idno><idno type="DOI">10.1016/S0044-8486(00)00355-0</idno><idno type="PII">S0044-8486(00)00355-0</idno></biblStruct></sourceDesc></fileDesc><profileDesc><creation><date>2000</date></creation><langUsage><language ident="en">en</language></langUsage><abstract xml:lang="en"><p>The optimal conditions for the retention of the second polar body in sea bass eggs were investigated by altering the timing, intensity and duration of application of pressure and cold shocks. Treatment optima for cold shocks were 0–1°C for 15–20 min at 5 min after fertilisation (a.f.) and 8500 psi for 2 min at 6 min a.f. for pressure shocks. Meiogenesis was obtained by fertilising eggs with UV-irradiated homologous sperm (32,000 erg mm−2) and pressure or cold shocking eggs as above. 100% triploidy was induced following definition of liable periods for the disruption of the meiotic spindle obtained in gynogenesis. Ploidy investigations were performed on experimental groups by flow-cytometry. Verification of uniparental transmission in meiogens was carried out by microsatellite marker loci analysis. This work highlights the degree of variation in individual responses of selected broodstock to these agents. Finally, some preliminary results on heterologous fertilisation in sea bass with potential applications for gynogenetic studies are also provided.</p></abstract><textClass><keywords scheme="keyword"><list><head>Keywords</head><item><term>Meiotic gynogenesis</term></item><item><term>Triploidy</term></item><item><term>Sea bass</term></item><item><term>Dicentrarchus labrax</term></item><item><term>Pressure and cold shocks</term></item><item><term>Parental variability</term></item></list></keywords></textClass></profileDesc><revisionDesc><change when="2000">Published</change></revisionDesc></teiHeader></istex:fulltextTEI><json:item><extension>txt</extension><original>false</original><mimetype>text/plain</mimetype><uri>https://api.istex.fr/document/137D45329C8625CEAF8D45F18F986669F4B64709/fulltext/txt</uri></json:item></fulltext><metadata><istex:metadataXml wicri:clean="Elsevier, elements deleted: ce:floats; body; tail"><istex:xmlDeclaration>version="1.0" encoding="utf-8"</istex:xmlDeclaration><istex:docType PUBLIC="-//ES//DTD journal article DTD version 4.5.2//EN//XML" URI="art452.dtd" name="istex:docType"><istex:entity SYSTEM="gr1" NDATA="IMAGE" name="gr1"></istex:entity><istex:entity SYSTEM="gr2" NDATA="IMAGE" name="gr2"></istex:entity><istex:entity SYSTEM="gr3" NDATA="IMAGE" name="gr3"></istex:entity><istex:entity SYSTEM="gr4" NDATA="IMAGE" name="gr4"></istex:entity><istex:entity SYSTEM="gr5" NDATA="IMAGE" name="gr5"></istex:entity><istex:entity SYSTEM="gr6" NDATA="IMAGE" name="gr6"></istex:entity></istex:docType><istex:document><converted-article version="4.5.2" docsubtype="fla"><item-info><jid>AQUA</jid><aid>61259</aid><ce:pii>S0044-8486(00)00355-0</ce:pii><ce:doi>10.1016/S0044-8486(00)00355-0</ce:doi><ce:copyright type="full-transfer" year="2000">Elsevier Science B.V.</ce:copyright></item-info><head><ce:title>Pressure and cold shock induction of meiotic gynogenesis and triploidy in the European sea bass, <ce:italic>Dicentrarchus labrax</ce:italic> L.: relative efficiency of methods and parental variability</ce:title><ce:author-group><ce:author><ce:given-name>Stefano</ce:given-name><ce:surname>Peruzzi</ce:surname><ce:cross-ref refid="AFF1"><ce:sup>a</ce:sup></ce:cross-ref></ce:author><ce:author><ce:given-name>Beatrice</ce:given-name><ce:surname>Chatain</ce:surname><ce:cross-ref refid="AFF2"><ce:sup>b</ce:sup></ce:cross-ref><ce:cross-ref refid="CORR1">*</ce:cross-ref><ce:e-address>bchatain@ifremer.fr</ce:e-address></ce:author><ce:affiliation id="AFF1"><ce:label>a</ce:label><ce:textfn>CEFREM, Centre de Formation et de Recherche sur l'Environnement Marin, CNRS UMR 5110, Université de Perpignan, 52 avenue de Villeneuve, 66860 Perpignan, France</ce:textfn></ce:affiliation><ce:affiliation id="AFF2"><ce:label>b</ce:label><ce:textfn>Station Expérimentale d'Acquaculture IFREMER, chemin de Maguelone, 34250 Palavas-les-Flots, France</ce:textfn></ce:affiliation><ce:correspondence id="CORR1"><ce:label>*</ce:label><ce:text>Corresponding author. Tel.: +33-4675-041-09; fax: +33-4676-828-85</ce:text></ce:correspondence></ce:author-group><ce:date-received day="7" month="3" year="1999"></ce:date-received><ce:date-accepted day="19" month="2" year="2000"></ce:date-accepted><ce:abstract><ce:section-title>Abstract</ce:section-title><ce:abstract-sec><ce:simple-para>The optimal conditions for the retention of the second polar body in sea bass eggs were investigated by altering the timing, intensity and duration of application of pressure and cold shocks. Treatment optima for cold shocks were 0–1°C for 15–20 min at 5 min after fertilisation (a.f.) and 8500 psi for 2 min at 6 min a.f. for pressure shocks. Meiogenesis was obtained by fertilising eggs with UV-irradiated homologous sperm (32,000 erg mm<ce:sup>−2</ce:sup>) and pressure or cold shocking eggs as above. 100% triploidy was induced following definition of liable periods for the disruption of the meiotic spindle obtained in gynogenesis. Ploidy investigations were performed on experimental groups by flow-cytometry. Verification of uniparental transmission in meiogens was carried out by microsatellite marker loci analysis. This work highlights the degree of variation in individual responses of selected broodstock to these agents. Finally, some preliminary results on heterologous fertilisation in sea bass with potential applications for gynogenetic studies are also provided.</ce:simple-para></ce:abstract-sec></ce:abstract><ce:keywords class="keyword"><ce:section-title>Keywords</ce:section-title><ce:keyword><ce:text>Meiotic gynogenesis</ce:text></ce:keyword><ce:keyword><ce:text>Triploidy</ce:text></ce:keyword><ce:keyword><ce:text>Sea bass</ce:text></ce:keyword><ce:keyword><ce:text><ce:italic>Dicentrarchus labrax</ce:italic></ce:text></ce:keyword><ce:keyword><ce:text>Pressure and cold shocks</ce:text></ce:keyword><ce:keyword><ce:text>Parental variability</ce:text></ce:keyword></ce:keywords></head></converted-article></istex:document></istex:metadataXml><mods version="3.6"><titleInfo><title>Pressure and cold shock induction of meiotic gynogenesis and triploidy in the European sea bass, Dicentrarchus labrax L.: relative efficiency of methods and parental variability</title></titleInfo><titleInfo type="alternative" contentType="CDATA"><title>Pressure and cold shock induction of meiotic gynogenesis and triploidy in the European sea bass,</title></titleInfo><name type="personal"><namePart type="given">Stefano</namePart><namePart type="family">Peruzzi</namePart><affiliation>CEFREM, Centre de Formation et de Recherche sur l'Environnement Marin, CNRS UMR 5110, Université de Perpignan, 52 avenue de Villeneuve, 66860 Perpignan, France</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">Beatrice</namePart><namePart type="family">Chatain</namePart><affiliation>Station Expérimentale d'Acquaculture IFREMER, chemin de Maguelone, 34250 Palavas-les-Flots, France</affiliation><affiliation>Corresponding author. Tel.: +33-4675-041-09; fax: +33-4676-828-85</affiliation><affiliation>E-mail: bchatain@ifremer.fr</affiliation><role><roleTerm type="text">author</roleTerm></role></name><typeOfResource>text</typeOfResource><genre type="research-article" displayLabel="Full-length article"></genre><originInfo><publisher>ELSEVIER</publisher><dateIssued encoding="w3cdtf">2000</dateIssued><copyrightDate encoding="w3cdtf">2000</copyrightDate></originInfo><language><languageTerm type="code" authority="iso639-2b">eng</languageTerm><languageTerm type="code" authority="rfc3066">en</languageTerm></language><physicalDescription><internetMediaType>text/html</internetMediaType></physicalDescription><abstract lang="en">The optimal conditions for the retention of the second polar body in sea bass eggs were investigated by altering the timing, intensity and duration of application of pressure and cold shocks. Treatment optima for cold shocks were 0–1°C for 15–20 min at 5 min after fertilisation (a.f.) and 8500 psi for 2 min at 6 min a.f. for pressure shocks. Meiogenesis was obtained by fertilising eggs with UV-irradiated homologous sperm (32,000 erg mm−2) and pressure or cold shocking eggs as above. 100% triploidy was induced following definition of liable periods for the disruption of the meiotic spindle obtained in gynogenesis. Ploidy investigations were performed on experimental groups by flow-cytometry. Verification of uniparental transmission in meiogens was carried out by microsatellite marker loci analysis. This work highlights the degree of variation in individual responses of selected broodstock to these agents. Finally, some preliminary results on heterologous fertilisation in sea bass with potential applications for gynogenetic studies are also provided.</abstract><note type="content">Fig. 1: Survival of D. labrax eggs fertilised with UV irradiated sperm. Means with a common superscript are not significantly different by χ2 test (P<0.05). Results observed at 4 h after fertilisation. (a) Mean and standard error of survival rates for the four tested couples. 2n: diploid status; n: haploid status. (b) Survival rate in each couple relative to control. At this stage, survival rates in control groups were of 90%, 83%, 98% and 90% for couples 1, 2, 3 and 4, respectively. Results observed at 72 h after fertilisation. (c) Mean and standard error of survival rate for the four tested couples. 2n: diploid status; n: haploid status. (d) Survival rate in each couple relative to control. At this stage, survival rates in control groups were of 56%, 70%, 69% and 53% for couples 1, 2, 3 and 4, respectively.</note><note type="content">Fig. 2: D. labrax haploid, diploid and triploid larvae and their respective nuclear DNA content measured by flow cytometry (M1, M2, M3). DNA values are reported in arbitrary units expressed as fluorescence channel numbers (FL2-Area). Scale bars represent 1 mm.</note><note type="content">Fig. 3: Survival of D. labrax eggs activated with homologous and heterologous sperm, from fertilisation to hatching. Spawning were issued from two different females (female 1: –; female 2: ---). • D. labrax sperm. ○ D. labrax irradiated sperm (total UV dose of 32.000 erg mm−2). ▴ Sparus aurata sperm.</note><note type="content">Fig. 4: Survival of D. labrax eggs fertilised with UV irradiated sperm (total UV dose of 32.000 erg mm−2). Eggs were submitted to a pressure shock (▩, 8000 psi for 2 min) or to a cold shock (□, 1°C for 20 min) applied at different moments after fertilisation. Results represent survival rates observed 72 h after fertilisation. Means with a common superscript are not significantly different by χ2 test (P<0.05). (a) Mean and standard error of survival rates for the four tested couples. 2n: diploid status; n:haploid status; ▨, diploid control groups. (b) Survival rate in each couple relative to control. At this stage, survival rates in control groups were of 69%, 66%, 97% and 70% for couples 1, 2, 3 and 4, respectively.</note><note type="content">Fig. 5: Observed Labrax 17 DNA banding pattern in control and gynogenetic progenies of D. labrax. Meiotic gynogens homozygous for one of the maternal alleles are indicated by arrows. F: female parent; M: male parent; bp: allele sizes given in base pairs.</note><note type="content">Fig. 6: Survival of D. labrax eggs fertilised with non-irradiated sperm. Eggs were submitted to pressure or cold shocks of different levels. Pressure shocks (▩) were applied 6 min after fertilisation for 2 min. Cold shocks of 1°C (□) were applied 5 min after fertilisation. Results represent the survival rates observed 72 h after fertilisation. Means with a common superscript are not significantly different by χ2 test (P<0.05). (a) Mean and standard error of survival rates for the three tested couples. 2n: diploid status; 3n: triploid status; ▨, diploid control groups. (b) Survival rates in each couple relative to control. At this stage, survival rates in control groups were of 76%, 95% and 88% for couples 1, 2, 3 and 4, respectively.</note><subject><genre>Keywords</genre><topic>Meiotic gynogenesis</topic><topic>Triploidy</topic><topic>Sea bass</topic><topic>Dicentrarchus labrax</topic><topic>Pressure and cold shocks</topic><topic>Parental variability</topic></subject><relatedItem type="host"><titleInfo><title>Aquaculture</title></titleInfo><titleInfo type="abbreviated"><title>AQUA</title></titleInfo><genre type="journal">journal</genre><originInfo><dateIssued encoding="w3cdtf">20000925</dateIssued></originInfo><identifier type="ISSN">0044-8486</identifier><identifier type="PII">S0044-8486(00)X0113-5</identifier><part><date>20000925</date><detail type="volume"><number>189</number><caption>vol.</caption></detail><detail type="issue"><number>1–2</number><caption>no.</caption></detail><extent unit="issue pages"><start>1</start><end>196</end></extent><extent unit="pages"><start>23</start><end>37</end></extent></part></relatedItem><identifier type="istex">137D45329C8625CEAF8D45F18F986669F4B64709</identifier><identifier type="DOI">10.1016/S0044-8486(00)00355-0</identifier><identifier type="PII">S0044-8486(00)00355-0</identifier><accessCondition type="use and reproduction" contentType="copyright">©2000 Elsevier Science B.V.</accessCondition><recordInfo><recordContentSource>ELSEVIER</recordContentSource><recordOrigin>Elsevier Science B.V., ©2000</recordOrigin></recordInfo></mods></metadata></istex></record>Pour manipuler ce document sous Unix (Dilib)
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