Improved Cryopreservation of Sperm of Paddlefish (Polyodon spathula)
Identifieur interne : 000B75 ( Istex/Corpus ); précédent : 000B74; suivant : 000B76Improved Cryopreservation of Sperm of Paddlefish (Polyodon spathula)
Auteurs : Ákos Horváth ; Béla Urbányi ; Steven D. Mims ; William B. Bean ; Boris Gomelsky ; Terrence R. TierschSource :
- Journal of the World Aquaculture Society [ 0893-8849 ] ; 2006-12.
Abstract
Experiments were performed to improve protocols for sperm cryopreservation of paddlefish (Polyodon spathula), a species for which there has been limited study. The first experiment was conducted to investigate the effects of two extenders (modified Tsvetkova’s extender: mT and modified Hanks’ balanced salt solution: mHBSS) in combination with methanol (MeOH) and dimethyl sulfoxide in two concentrations (5 and 10%) on the postthaw motility and fertilization rates of cryopreserved sperm. The highest postthaw motility (85 ± 5%) was observed when sperm were frozen using mT extender with 10% MeOH as cryoprotectant. Extenders (P = 0.0018) and cryoprotectants (P = 0.0040) each had a significant effect on the postthaw motility of paddlefish sperm. The highest fertilization (80 ± 3%) was found when eggs were fertilized with sperm frozen with mT extender in combination with 10% MeOH. However, there was no significant difference among fertilization rates when MeOH was used as a cryoprotectant in either concentration or in combination with either mT or mHBSS extenders. In the second experiment, 4000 eggs were fertilized with the pooled contents of five straws of thawed sperm (total volume of 1.25 mL) using mT extender in combination with 5% MeOH, and hatch rates as high as 79 ± 5% were observed. A third experiment was also conducted to clarify the role of MeOH concentration; however, no significant difference was found among fertilization and hatch rates when either 5 or 10% MeOH was used as a cryoprotectant. These results suggest that MeOH is a safe and reliable cryoprotectant for freezing of paddlefish sperm and obtaining viable postthaw sperm for consistent fertilization and hatch rates. Further, this experimental protocol is relatively simple and applicable for commercial hatchery production of paddlefish.
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DOI: 10.1111/j.1749-7345.2006.00048.x
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Mims</name><affiliation><mods:affiliation>Division of Aquaculture and Aquatic Sciences, Aquaculture Research Center, Frankfort, Kentucky 40601 USA</mods:affiliation></affiliation></author><author><name sortKey="Bean, William B" sort="Bean, William B" uniqKey="Bean W" first="William B." last="Bean">William B. Bean</name><affiliation><mods:affiliation>Division of Aquaculture and Aquatic Sciences, Aquaculture Research Center, Frankfort, Kentucky 40601 USA</mods:affiliation></affiliation></author><author><name sortKey="Gomelsky, Boris" sort="Gomelsky, Boris" uniqKey="Gomelsky B" first="Boris" last="Gomelsky">Boris Gomelsky</name><affiliation><mods:affiliation>Division of Aquaculture and Aquatic Sciences, Aquaculture Research Center, Frankfort, Kentucky 40601 USA</mods:affiliation></affiliation></author><author><name sortKey="Tiersch, Terrence R" sort="Tiersch, Terrence R" uniqKey="Tiersch T" first="Terrence R." last="Tiersch">Terrence R. Tiersch</name><affiliation><mods:affiliation>Aquaculture Research Station, Louisiana Agricultural Experiment Station, Louisiana State University Agricultural Center, Baton Rouge, Louisiana 70820 USA</mods:affiliation></affiliation></author></analytic><monogr></monogr><series><title level="j">Journal of the World Aquaculture Society</title><idno type="ISSN">0893-8849</idno><idno type="eISSN">1749-7345</idno><imprint><publisher>Blackwell Publishing Inc</publisher><pubPlace>Malden, USA</pubPlace><date type="published" when="2006-12">2006-12</date><biblScope unit="volume">37</biblScope><biblScope unit="issue">4</biblScope><biblScope unit="page" from="356">356</biblScope><biblScope unit="page" to="362">362</biblScope></imprint><idno type="ISSN">0893-8849</idno></series><idno type="istex">1E9D01257BD572BBBEF1556C087B82626D771AFE</idno><idno type="DOI">10.1111/j.1749-7345.2006.00048.x</idno><idno type="ArticleID">JWAS48</idno></biblStruct></sourceDesc><seriesStmt><idno type="ISSN">0893-8849</idno></seriesStmt></fileDesc><profileDesc><textClass></textClass><langUsage><language ident="en">en</language></langUsage></profileDesc></teiHeader><front><div type="abstract">Experiments were performed to improve protocols for sperm cryopreservation of paddlefish (Polyodon spathula), a species for which there has been limited study. The first experiment was conducted to investigate the effects of two extenders (modified Tsvetkova’s extender: mT and modified Hanks’ balanced salt solution: mHBSS) in combination with methanol (MeOH) and dimethyl sulfoxide in two concentrations (5 and 10%) on the postthaw motility and fertilization rates of cryopreserved sperm. The highest postthaw motility (85 ± 5%) was observed when sperm were frozen using mT extender with 10% MeOH as cryoprotectant. Extenders (P = 0.0018) and cryoprotectants (P = 0.0040) each had a significant effect on the postthaw motility of paddlefish sperm. The highest fertilization (80 ± 3%) was found when eggs were fertilized with sperm frozen with mT extender in combination with 10% MeOH. However, there was no significant difference among fertilization rates when MeOH was used as a cryoprotectant in either concentration or in combination with either mT or mHBSS extenders. In the second experiment, 4000 eggs were fertilized with the pooled contents of five straws of thawed sperm (total volume of 1.25 mL) using mT extender in combination with 5% MeOH, and hatch rates as high as 79 ± 5% were observed. A third experiment was also conducted to clarify the role of MeOH concentration; however, no significant difference was found among fertilization and hatch rates when either 5 or 10% MeOH was used as a cryoprotectant. These results suggest that MeOH is a safe and reliable cryoprotectant for freezing of paddlefish sperm and obtaining viable postthaw sperm for consistent fertilization and hatch rates. Further, this experimental protocol is relatively simple and applicable for commercial hatchery production of paddlefish.</div></front></TEI><istex><corpusName>wiley</corpusName><author><json:item><name>ÁKos Horváth</name><affiliations><json:string>Department of Fish Culture, Szent István University, Páter K. u. 1., Gödöllő H‐2103 Hungary, and Aquaculture Research Station, Louisiana Agricultural Experiment Station, Louisiana State University Agricultural Center, Baton Rouge, Louisiana 70820 USA</json:string><json:string>Corresponding author.</json:string></affiliations></json:item><json:item><name>Béla Urbányi</name><affiliations><json:string>Department of Fish Culture, Szent István University, Páter K. u. 1., Gödöllő H‐2103 Hungary</json:string></affiliations></json:item><json:item><name>Steven D. Mims</name><affiliations><json:string>Division of Aquaculture and Aquatic Sciences, Aquaculture Research Center, Frankfort, Kentucky 40601 USA</json:string></affiliations></json:item><json:item><name>William B. Bean</name><affiliations><json:string>Division of Aquaculture and Aquatic Sciences, Aquaculture Research Center, Frankfort, Kentucky 40601 USA</json:string></affiliations></json:item><json:item><name>Boris Gomelsky</name><affiliations><json:string>Division of Aquaculture and Aquatic Sciences, Aquaculture Research Center, Frankfort, Kentucky 40601 USA</json:string></affiliations></json:item><json:item><name>Terrence R. Tiersch</name><affiliations><json:string>Aquaculture Research Station, Louisiana Agricultural Experiment Station, Louisiana State University Agricultural Center, Baton Rouge, Louisiana 70820 USA</json:string></affiliations></json:item></author><articleId><json:string>JWAS48</json:string></articleId><language><json:string>eng</json:string></language><originalGenre><json:string>article</json:string></originalGenre><abstract>Experiments were performed to improve protocols for sperm cryopreservation of paddlefish (Polyodon spathula), a species for which there has been limited study. The first experiment was conducted to investigate the effects of two extenders (modified Tsvetkova’s extender: mT and modified Hanks’ balanced salt solution: mHBSS) in combination with methanol (MeOH) and dimethyl sulfoxide in two concentrations (5 and 10%) on the postthaw motility and fertilization rates of cryopreserved sperm. The highest postthaw motility (85 ± 5%) was observed when sperm were frozen using mT extender with 10% MeOH as cryoprotectant. Extenders (P = 0.0018) and cryoprotectants (P = 0.0040) each had a significant effect on the postthaw motility of paddlefish sperm. The highest fertilization (80 ± 3%) was found when eggs were fertilized with sperm frozen with mT extender in combination with 10% MeOH. However, there was no significant difference among fertilization rates when MeOH was used as a cryoprotectant in either concentration or in combination with either mT or mHBSS extenders. In the second experiment, 4000 eggs were fertilized with the pooled contents of five straws of thawed sperm (total volume of 1.25 mL) using mT extender in combination with 5% MeOH, and hatch rates as high as 79 ± 5% were observed. A third experiment was also conducted to clarify the role of MeOH concentration; however, no significant difference was found among fertilization and hatch rates when either 5 or 10% MeOH was used as a cryoprotectant. These results suggest that MeOH is a safe and reliable cryoprotectant for freezing of paddlefish sperm and obtaining viable postthaw sperm for consistent fertilization and hatch rates. 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István University, Páter K. u. 1., Gödöllő H‐2103 Hungary</affiliation></author><author xml:id="author-3"><persName><forename type="first">Steven D.</forename><surname>Mims</surname></persName><affiliation>Division of Aquaculture and Aquatic Sciences, Aquaculture Research Center, Frankfort, Kentucky 40601 USA</affiliation></author><author xml:id="author-4"><persName><forename type="first">William B.</forename><surname>Bean</surname></persName><affiliation>Division of Aquaculture and Aquatic Sciences, Aquaculture Research Center, Frankfort, Kentucky 40601 USA</affiliation></author><author xml:id="author-5"><persName><forename type="first">Boris</forename><surname>Gomelsky</surname></persName><affiliation>Division of Aquaculture and Aquatic Sciences, Aquaculture Research Center, Frankfort, Kentucky 40601 USA</affiliation></author><author xml:id="author-6"><persName><forename type="first">Terrence R.</forename><surname>Tiersch</surname></persName><affiliation>Aquaculture Research Station, Louisiana Agricultural Experiment Station, Louisiana State University Agricultural Center, Baton Rouge, Louisiana 70820 USA</affiliation></author></analytic><monogr><title level="j">Journal of the World Aquaculture Society</title><idno type="pISSN">0893-8849</idno><idno type="eISSN">1749-7345</idno><idno type="DOI">10.1111/(ISSN)1749-7345</idno><imprint><publisher>Blackwell Publishing Inc</publisher><pubPlace>Malden, USA</pubPlace><date type="published" when="2006-12"></date><biblScope unit="volume">37</biblScope><biblScope unit="issue">4</biblScope><biblScope unit="page" from="356">356</biblScope><biblScope unit="page" to="362">362</biblScope></imprint></monogr><idno type="istex">1E9D01257BD572BBBEF1556C087B82626D771AFE</idno><idno type="DOI">10.1111/j.1749-7345.2006.00048.x</idno><idno type="ArticleID">JWAS48</idno></biblStruct></sourceDesc></fileDesc><profileDesc><creation><date>2006</date></creation><langUsage><language ident="en">en</language></langUsage><abstract><p>Experiments were performed to improve protocols for sperm cryopreservation of paddlefish (Polyodon spathula), a species for which there has been limited study. The first experiment was conducted to investigate the effects of two extenders (modified Tsvetkova’s extender: mT and modified Hanks’ balanced salt solution: mHBSS) in combination with methanol (MeOH) and dimethyl sulfoxide in two concentrations (5 and 10%) on the postthaw motility and fertilization rates of cryopreserved sperm. The highest postthaw motility (85 ± 5%) was observed when sperm were frozen using mT extender with 10% MeOH as cryoprotectant. Extenders (P = 0.0018) and cryoprotectants (P = 0.0040) each had a significant effect on the postthaw motility of paddlefish sperm. The highest fertilization (80 ± 3%) was found when eggs were fertilized with sperm frozen with mT extender in combination with 10% MeOH. However, there was no significant difference among fertilization rates when MeOH was used as a cryoprotectant in either concentration or in combination with either mT or mHBSS extenders. In the second experiment, 4000 eggs were fertilized with the pooled contents of five straws of thawed sperm (total volume of 1.25 mL) using mT extender in combination with 5% MeOH, and hatch rates as high as 79 ± 5% were observed. A third experiment was also conducted to clarify the role of MeOH concentration; however, no significant difference was found among fertilization and hatch rates when either 5 or 10% MeOH was used as a cryoprotectant. These results suggest that MeOH is a safe and reliable cryoprotectant for freezing of paddlefish sperm and obtaining viable postthaw sperm for consistent fertilization and hatch rates. Further, this experimental protocol is relatively simple and applicable for commercial hatchery production of paddlefish.</p></abstract></profileDesc><revisionDesc><change when="2006-12">Published</change></revisionDesc></teiHeader></istex:fulltextTEI><json:item><extension>txt</extension><original>false</original><mimetype>text/plain</mimetype><uri>https://api.istex.fr/document/1E9D01257BD572BBBEF1556C087B82626D771AFE/fulltext/txt</uri></json:item></fulltext><metadata><istex:metadataXml wicri:clean="Wiley, elements deleted: body"><istex:xmlDeclaration>version="1.0" encoding="UTF-8" standalone="yes"</istex:xmlDeclaration><istex:document><component version="2.0" type="serialArticle" xml:lang="en"><header><publicationMeta level="product"><publisherInfo><publisherName>Blackwell Publishing Inc</publisherName><publisherLoc>Malden, USA</publisherLoc></publisherInfo><doi origin="wiley" registered="yes">10.1111/(ISSN)1749-7345</doi><issn type="print">0893-8849</issn><issn 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xml:id="cr5" affiliationRef="#a3"><personName><givenNames>Boris</givenNames><familyName>Gomelsky</familyName></personName></creator><creator creatorRole="author" xml:id="cr6" affiliationRef="#a4"><personName><givenNames>Terrence R.</givenNames><familyName>Tiersch</familyName></personName></creator></creators><affiliationGroup><affiliation xml:id="a1" countryCode="US"><unparsedAffiliation><i>Department of Fish Culture, Szent István University, Páter K. u. 1., Gödöllő H‐2103 Hungary, and Aquaculture Research Station, Louisiana Agricultural Experiment Station, Louisiana State University Agricultural Center, Baton Rouge, Louisiana 70820 USA</i></unparsedAffiliation></affiliation><affiliation xml:id="a2" countryCode="HU"><unparsedAffiliation><i>Department of Fish Culture, Szent István University, Páter K. u. 1., Gödöllő H‐2103 Hungary</i></unparsedAffiliation></affiliation><affiliation xml:id="a3" countryCode="US"><unparsedAffiliation><i>Division of Aquaculture and Aquatic Sciences, Aquaculture Research Center, Frankfort, Kentucky 40601 USA</i></unparsedAffiliation></affiliation><affiliation xml:id="a4" countryCode="US"><unparsedAffiliation><i>Aquaculture Research Station, Louisiana Agricultural Experiment Station, Louisiana State University Agricultural Center, Baton Rouge, Louisiana 70820 USA</i></unparsedAffiliation></affiliation></affiliationGroup><abstractGroup><abstract type="main" xml:lang="en"><title type="main">Abstract</title><p> <b>Experiments were performed to improve protocols for sperm cryopreservation of paddlefish (<i>Polyodon spathula</i>), a species for which there has been limited study. The first experiment was conducted to investigate the effects of two extenders (modified Tsvetkova’s extender: mT and modified Hanks’ balanced salt solution: mHBSS) in combination with methanol (MeOH) and dimethyl sulfoxide in two concentrations (5 and 10%) on the postthaw motility and fertilization rates of cryopreserved sperm. The highest postthaw motility (85 ± 5%) was observed when sperm were frozen using mT extender with 10% MeOH as cryoprotectant. Extenders (<i>P</i> = 0.0018) and cryoprotectants (<i>P</i> = 0.0040) each had a significant effect on the postthaw motility of paddlefish sperm. The highest fertilization (80 ± 3%) was found when eggs were fertilized with sperm frozen with mT extender in combination with 10% MeOH. However, there was no significant difference among fertilization rates when MeOH was used as a cryoprotectant in either concentration or in combination with either mT or mHBSS extenders. In the second experiment, 4000 eggs were fertilized with the pooled contents of five straws of thawed sperm (total volume of 1.25 mL) using mT extender in combination with 5% MeOH, and hatch rates as high as 79 ± 5% were observed. A third experiment was also conducted to clarify the role of MeOH concentration; however, no significant difference was found among fertilization and hatch rates when either 5 or 10% MeOH was used as a cryoprotectant. These results suggest that MeOH is a safe and reliable cryoprotectant for freezing of paddlefish sperm and obtaining viable postthaw sperm for consistent fertilization and hatch rates. Further, this experimental protocol is relatively simple and applicable for commercial hatchery production of paddlefish.</b> </p></abstract></abstractGroup></contentMeta></header></component></istex:document></istex:metadataXml><mods version="3.6"><titleInfo lang="en"><title>Improved Cryopreservation of Sperm of Paddlefish (Polyodon spathula)</title></titleInfo><titleInfo type="alternative" contentType="CDATA" lang="en"><title>Improved Cryopreservation of Sperm of Paddlefish (Polyodon spathula)</title></titleInfo><name type="personal"><namePart type="given">ÁKos</namePart><namePart type="family">Horváth</namePart><affiliation>Department of Fish Culture, Szent István University, Páter K. u. 1., Gödöllő H‐2103 Hungary, and Aquaculture Research Station, Louisiana Agricultural Experiment Station, Louisiana State University Agricultural Center, Baton Rouge, Louisiana 70820 USA</affiliation><affiliation> Corresponding author.</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">Béla</namePart><namePart type="family">Urbányi</namePart><affiliation>Department of Fish Culture, Szent István University, Páter K. u. 1., Gödöllő H‐2103 Hungary</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">Steven D.</namePart><namePart type="family">Mims</namePart><affiliation>Division of Aquaculture and Aquatic Sciences, Aquaculture Research Center, Frankfort, Kentucky 40601 USA</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">William B.</namePart><namePart type="family">Bean</namePart><affiliation>Division of Aquaculture and Aquatic Sciences, Aquaculture Research Center, Frankfort, Kentucky 40601 USA</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">Boris</namePart><namePart type="family">Gomelsky</namePart><affiliation>Division of Aquaculture and Aquatic Sciences, Aquaculture Research Center, Frankfort, Kentucky 40601 USA</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">Terrence R.</namePart><namePart type="family">Tiersch</namePart><affiliation>Aquaculture Research Station, Louisiana Agricultural Experiment Station, Louisiana State University Agricultural Center, Baton Rouge, Louisiana 70820 USA</affiliation><role><roleTerm type="text">author</roleTerm></role></name><typeOfResource>text</typeOfResource><genre type="article" displayLabel="article"></genre><originInfo><publisher>Blackwell Publishing Inc</publisher><place><placeTerm type="text">Malden, USA</placeTerm></place><dateIssued encoding="w3cdtf">2006-12</dateIssued><copyrightDate encoding="w3cdtf">2006</copyrightDate></originInfo><language><languageTerm type="code" authority="rfc3066">en</languageTerm><languageTerm type="code" authority="iso639-2b">eng</languageTerm></language><physicalDescription><internetMediaType>text/html</internetMediaType><extent unit="tables">3</extent><extent unit="references">26</extent></physicalDescription><abstract>Experiments were performed to improve protocols for sperm cryopreservation of paddlefish (Polyodon spathula), a species for which there has been limited study. The first experiment was conducted to investigate the effects of two extenders (modified Tsvetkova’s extender: mT and modified Hanks’ balanced salt solution: mHBSS) in combination with methanol (MeOH) and dimethyl sulfoxide in two concentrations (5 and 10%) on the postthaw motility and fertilization rates of cryopreserved sperm. The highest postthaw motility (85 ± 5%) was observed when sperm were frozen using mT extender with 10% MeOH as cryoprotectant. Extenders (P = 0.0018) and cryoprotectants (P = 0.0040) each had a significant effect on the postthaw motility of paddlefish sperm. The highest fertilization (80 ± 3%) was found when eggs were fertilized with sperm frozen with mT extender in combination with 10% MeOH. However, there was no significant difference among fertilization rates when MeOH was used as a cryoprotectant in either concentration or in combination with either mT or mHBSS extenders. In the second experiment, 4000 eggs were fertilized with the pooled contents of five straws of thawed sperm (total volume of 1.25 mL) using mT extender in combination with 5% MeOH, and hatch rates as high as 79 ± 5% were observed. A third experiment was also conducted to clarify the role of MeOH concentration; however, no significant difference was found among fertilization and hatch rates when either 5 or 10% MeOH was used as a cryoprotectant. These results suggest that MeOH is a safe and reliable cryoprotectant for freezing of paddlefish sperm and obtaining viable postthaw sperm for consistent fertilization and hatch rates. Further, this experimental protocol is relatively simple and applicable for commercial hatchery production of paddlefish.</abstract><relatedItem type="host"><titleInfo><title>Journal of the World Aquaculture Society</title></titleInfo><genre type="journal">journal</genre><identifier type="ISSN">0893-8849</identifier><identifier type="eISSN">1749-7345</identifier><identifier type="DOI">10.1111/(ISSN)1749-7345</identifier><identifier type="PublisherID">JWAS</identifier><part><date>2006</date><detail type="volume"><caption>vol.</caption><number>37</number></detail><detail type="issue"><caption>no.</caption><number>4</number></detail><extent unit="pages"><start>356</start><end>362</end><total>7</total></extent></part></relatedItem><identifier type="istex">1E9D01257BD572BBBEF1556C087B82626D771AFE</identifier><identifier type="DOI">10.1111/j.1749-7345.2006.00048.x</identifier><identifier type="ArticleID">JWAS48</identifier><recordInfo><recordContentSource>WILEY</recordContentSource><recordOrigin>Blackwell Publishing Inc</recordOrigin></recordInfo></mods></metadata><serie></serie></istex></record>Pour manipuler ce document sous Unix (Dilib)
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