Fish Species Identification by Means of Restriction Fragment Length Polymorphism and High‐Performance Liquid Chromatography
Identifieur interne : 000982 ( Istex/Corpus ); précédent : 000981; suivant : 000983Fish Species Identification by Means of Restriction Fragment Length Polymorphism and High‐Performance Liquid Chromatography
Auteurs : B. Horstkotte ; H. RehbeinSource :
- Journal of Food Science [ 0022-1147 ] ; 2003-11.
Abstract
ABSTRACT The usability of fish species identification with restriction fragment length polymorphism (RFLP) using high‐performance liquid chromatography (HPLC) was evaluated for sturgeon, salmon, and tuna samples. Separations of restriction fragments were performed within about 10 min at alkaline pH using an anion‐ex‐change diethylaminoethyl nonporous column. Unequivocal species identification was achieved with HPLC, despite nucleotide sequence‐depending separation. Separation of DNA fragments by HPLC could be demonstrated to be a fast and reliable alternative to electrophoresis. Calculation of restriction fragment lengths using relative peak areas showed better accordance compared with values obtained by means of DNA size standards. Keywords: species identification, HPLC, RFLP, fish
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DOI: 10.1111/j.1365-2621.2003.tb05785.x
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Rehbein</name><affiliation><mods:affiliation>Authors Horstkotte and Rehbein are with the Federal Research Centre for Fisheries, Inst. for Fishery Technology and Fish Quality, Palmaille 9, 22335 Hamburg, Germany. Direct inquiries to author Horstkotte (E‐mail: Burkhard.Horstkotte@ibt.bfa‐fisch.de) or author Rehbein (E‐mail: Hartmut.Rehbein@ibt.bfa‐fisch.de).</mods:affiliation></affiliation></author></titleStmt><publicationStmt><idno type="wicri:source">ISTEX</idno><idno type="RBID">ISTEX:E3EC7896CD2C448CDC5B38F7E3AEC20638D00AAC</idno><date when="2003" year="2003">2003</date><idno type="doi">10.1111/j.1365-2621.2003.tb05785.x</idno><idno type="url">https://api.istex.fr/document/E3EC7896CD2C448CDC5B38F7E3AEC20638D00AAC/fulltext/pdf</idno><idno type="wicri:Area/Istex/Corpus">000982</idno><idno type="wicri:explorRef" wicri:stream="Istex" wicri:step="Corpus" wicri:corpus="ISTEX">000982</idno></publicationStmt><sourceDesc><biblStruct><analytic><title level="a" type="main" xml:lang="en">Fish Species Identification by Means of Restriction Fragment Length Polymorphism and High‐Performance Liquid Chromatography</title><author><name sortKey="Horstkotte, B" sort="Horstkotte, B" uniqKey="Horstkotte B" first="B." last="Horstkotte">B. Horstkotte</name><affiliation><mods:affiliation>Authors Horstkotte and Rehbein are with the Federal Research Centre for Fisheries, Inst. for Fishery Technology and Fish Quality, Palmaille 9, 22335 Hamburg, Germany. Direct inquiries to author Horstkotte (E‐mail: Burkhard.Horstkotte@ibt.bfa‐fisch.de) or author Rehbein (E‐mail: Hartmut.Rehbein@ibt.bfa‐fisch.de).</mods:affiliation></affiliation></author><author><name sortKey="Rehbein, H" sort="Rehbein, H" uniqKey="Rehbein H" first="H." last="Rehbein">H. Rehbein</name><affiliation><mods:affiliation>Authors Horstkotte and Rehbein are with the Federal Research Centre for Fisheries, Inst. for Fishery Technology and Fish Quality, Palmaille 9, 22335 Hamburg, Germany. Direct inquiries to author Horstkotte (E‐mail: Burkhard.Horstkotte@ibt.bfa‐fisch.de) or author Rehbein (E‐mail: Hartmut.Rehbein@ibt.bfa‐fisch.de).</mods:affiliation></affiliation></author></analytic><monogr></monogr><series><title level="j">Journal of Food Science</title><idno type="ISSN">0022-1147</idno><idno type="eISSN">1750-3841</idno><imprint><publisher>Blackwell Publishing Ltd</publisher><pubPlace>Oxford, UK</pubPlace><date type="published" when="2003-11">2003-11</date><biblScope unit="volume">68</biblScope><biblScope unit="issue">9</biblScope><biblScope unit="page" from="2658">2658</biblScope><biblScope unit="page" to="2666">2666</biblScope></imprint><idno type="ISSN">0022-1147</idno></series><idno type="istex">E3EC7896CD2C448CDC5B38F7E3AEC20638D00AAC</idno><idno type="DOI">10.1111/j.1365-2621.2003.tb05785.x</idno><idno type="ArticleID">JFDS2658</idno></biblStruct></sourceDesc><seriesStmt><idno type="ISSN">0022-1147</idno></seriesStmt></fileDesc><profileDesc><textClass></textClass><langUsage><language ident="en">en</language></langUsage></profileDesc></teiHeader><front><div type="abstract">ABSTRACT The usability of fish species identification with restriction fragment length polymorphism (RFLP) using high‐performance liquid chromatography (HPLC) was evaluated for sturgeon, salmon, and tuna samples. Separations of restriction fragments were performed within about 10 min at alkaline pH using an anion‐ex‐change diethylaminoethyl nonporous column. Unequivocal species identification was achieved with HPLC, despite nucleotide sequence‐depending separation. Separation of DNA fragments by HPLC could be demonstrated to be a fast and reliable alternative to electrophoresis. Calculation of restriction fragment lengths using relative peak areas showed better accordance compared with values obtained by means of DNA size standards. Keywords: species identification, HPLC, RFLP, fish</div></front></TEI><istex><corpusName>wiley</corpusName><author><json:item><name>B. HORSTKOTTE</name><affiliations><json:string>Authors Horstkotte and Rehbein are with the Federal Research Centre for Fisheries, Inst. for Fishery Technology and Fish Quality, Palmaille 9, 22335 Hamburg, Germany. 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Calculation of restriction fragment lengths using relative peak areas showed better accordance compared with values obtained by means of DNA size standards. 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Direct inquiries to author Horstkotte (E‐mail: <email normalForm="Burkhard.Horstkotte@ibt.bfa-fisch.de">Burkhard.Horstkotte@ibt.bfa‐fisch.de</email>) or author Rehbein (E‐mail: <email normalForm="Hartmut.Rehbein@ibt.bfa-fisch.de">Hartmut.Rehbein@ibt.bfa‐fisch.de</email>).</unparsedAffiliation></affiliation></affiliationGroup><abstractGroup><abstract type="main" xml:lang="en"><p><b>ABSTRACT</b> The usability of fish species identification with restriction fragment length polymorphism (RFLP) using high‐performance liquid chromatography (HPLC) was evaluated for sturgeon, salmon, and tuna samples. Separations of restriction fragments were performed within about 10 min at alkaline pH using an anion‐ex‐change diethylaminoethyl nonporous column. Unequivocal species identification was achieved with HPLC, despite nucleotide sequence‐depending separation. Separation of DNA fragments by HPLC could be demonstrated to be a fast and reliable alternative to electrophoresis. 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Direct inquiries to author Horstkotte (E‐mail: Burkhard.Horstkotte@ibt.bfa‐fisch.de) or author Rehbein (E‐mail: Hartmut.Rehbein@ibt.bfa‐fisch.de).</affiliation><role><roleTerm type="text">author</roleTerm></role></name><typeOfResource>text</typeOfResource><genre type="article" displayLabel="article"></genre><originInfo><publisher>Blackwell Publishing Ltd</publisher><place><placeTerm type="text">Oxford, UK</placeTerm></place><dateIssued encoding="w3cdtf">2003-11</dateIssued><edition>MS 20030347 Submitted 6/23/03, Revised 9/10/03, Accepted 9/29/03</edition><copyrightDate encoding="w3cdtf">2003</copyrightDate></originInfo><language><languageTerm type="code" authority="rfc3066">en</languageTerm><languageTerm type="code" authority="iso639-2b">eng</languageTerm></language><physicalDescription><internetMediaType>text/html</internetMediaType><extent unit="references">20</extent></physicalDescription><abstract>ABSTRACT The usability of fish species identification with restriction fragment length polymorphism (RFLP) using high‐performance liquid chromatography (HPLC) was evaluated for sturgeon, salmon, and tuna samples. Separations of restriction fragments were performed within about 10 min at alkaline pH using an anion‐ex‐change diethylaminoethyl nonporous column. Unequivocal species identification was achieved with HPLC, despite nucleotide sequence‐depending separation. Separation of DNA fragments by HPLC could be demonstrated to be a fast and reliable alternative to electrophoresis. Calculation of restriction fragment lengths using relative peak areas showed better accordance compared with values obtained by means of DNA size standards. 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