Urinary bladder, ionic composition of seminal fluid and urine with characterization of sperm motility in tench (Tinca tinca L.)
Identifieur interne : 000870 ( Istex/Corpus ); précédent : 000869; suivant : 000871Urinary bladder, ionic composition of seminal fluid and urine with characterization of sperm motility in tench (Tinca tinca L.)
Auteurs : O. Linhart ; M. Rodina ; J. Bastl ; J. CossonSource :
- Journal of Applied Ichthyology [ 0175-8659 ] ; 2003-06.
Abstract
A small urinary bladder attached to the seminal duct in caudal part of the abdominal cavity was registered for the first time in dissected males of tench. The urinary bladder wall was of whitish color and the bladder contained 0.5–2 ml of urine. When collected in the experiment, the tench sperm was white‐colored. Spermatozoa density is highly variable due to contamination by urine, and the latter additionally activates spontaneous motility of the spermatozoa. Seminal fluid contains ions such as Na+ (18.4 ± 1.3 mm), K+ (1.9 ± 0.6 mm), Ca2+ (0.6 ± 0.2 mm) and Mg2+ (0.5 ± 0.1 mm), leading to osmolality of 230 ± 82 mOsmol kg−1 depending on the dilution by urine. Urea was detected in urine samples uncontaminated by sperm with an osmolality of 85 ± 58 mOsmol kg−1. Urine also contained high concentrations of ions such as Na+ (30.9 ± 8.9 mm), K+ (4.3 ± 2.9 mm), Ca2+ (0.9 ± 0.5 mm) and Mg2+ (0.6 ± 0.2 mm). The spontaneous sperm activation by urine was up to 100%, but could be prevented by collection in an immobilizing solution. Motility was observed for 90–100% spermatozoa just after their transfer to distilled water or in a swimming medium (SM, 30–45 mm KCl) with a velocity of 120–140 μm s−1. A flagellar beat frequency of 60–70 Hz and forward motility lasted up to 80 s in distilled water, and up to 180 s in SM at room temperature.
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DOI: 10.1046/j.1439-0426.2003.00470.x
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The urinary bladder wall was of whitish color and the bladder contained 0.5–2 ml of urine. When collected in the experiment, the tench sperm was white‐colored. Spermatozoa density is highly variable due to contamination by urine, and the latter additionally activates spontaneous motility of the spermatozoa. Seminal fluid contains ions such as Na+ (18.4 ± 1.3 mm), K+ (1.9 ± 0.6 mm), Ca2+ (0.6 ± 0.2 mm) and Mg2+ (0.5 ± 0.1 mm), leading to osmolality of 230 ± 82 mOsmol kg−1 depending on the dilution by urine. Urea was detected in urine samples uncontaminated by sperm with an osmolality of 85 ± 58 mOsmol kg−1. Urine also contained high concentrations of ions such as Na+ (30.9 ± 8.9 mm), K+ (4.3 ± 2.9 mm), Ca2+ (0.9 ± 0.5 mm) and Mg2+ (0.6 ± 0.2 mm). The spontaneous sperm activation by urine was up to 100%, but could be prevented by collection in an immobilizing solution. Motility was observed for 90–100% spermatozoa just after their transfer to distilled water or in a swimming medium (SM, 30–45 mm KCl) with a velocity of 120–140 μm s−1. A flagellar beat frequency of 60–70 Hz and forward motility lasted up to 80 s in distilled water, and up to 180 s in SM at room temperature.</div></front></TEI><istex><corpusName>wiley</corpusName><author><json:item><name>O. Linhart</name><affiliations><json:string>Joint Laboratory of Genetics, Physiology and Reproduction of Fish, Institute of Animal Physiology and Genetics, Academy of Sciences of the Czech Republic, University of South Bohemia, Research Institute of Fish Culture and Hydrobiology, Vodnany, Czech Republic;</json:string></affiliations></json:item><json:item><name>M. 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The urinary bladder wall was of whitish color and the bladder contained 0.5–2 ml of urine. When collected in the experiment, the tench sperm was white‐colored. Spermatozoa density is highly variable due to contamination by urine, and the latter additionally activates spontaneous motility of the spermatozoa. Seminal fluid contains ions such as Na+ (18.4 ± 1.3 mm), K+ (1.9 ± 0.6 mm), Ca2+ (0.6 ± 0.2 mm) and Mg2+ (0.5 ± 0.1 mm), leading to osmolality of 230 ± 82 mOsmol kg−1 depending on the dilution by urine. Urea was detected in urine samples uncontaminated by sperm with an osmolality of 85 ± 58 mOsmol kg−1. Urine also contained high concentrations of ions such as Na+ (30.9 ± 8.9 mm), K+ (4.3 ± 2.9 mm), Ca2+ (0.9 ± 0.5 mm) and Mg2+ (0.6 ± 0.2 mm). The spontaneous sperm activation by urine was up to 100%, but could be prevented by collection in an immobilizing solution. Motility was observed for 90–100% spermatozoa just after their transfer to distilled water or in a swimming medium (SM, 30–45 mm KCl) with a velocity of 120–140 μm s−1. 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Linhart et al.</title><title type="short">Characterization of sperm motility in tench (<i>Tinca tinca</i> L.)</title></titleGroup><creators><creator creatorRole="author" xml:id="cr1" affiliationRef="#a1"><personName><givenNames>O.</givenNames><familyName>Linhart</familyName></personName></creator><creator creatorRole="author" xml:id="cr2" affiliationRef="#a1"><personName><givenNames>M.</givenNames><familyName>Rodina</familyName></personName></creator><creator creatorRole="author" xml:id="cr3" affiliationRef="#a2"><personName><givenNames>J.</givenNames><familyName>Bastl</familyName></personName></creator><creator creatorRole="author" xml:id="cr4" affiliationRef="#a3"><personName><givenNames>J.</givenNames><familyName>Cosson</familyName></personName></creator></creators><affiliationGroup><affiliation xml:id="a1" countryCode="CZ"><unparsedAffiliation>Joint Laboratory of Genetics, Physiology and Reproduction of Fish, Institute of Animal Physiology and Genetics, Academy of Sciences of the Czech Republic, University of South Bohemia, Research Institute of Fish Culture and Hydrobiology, Vodnany, Czech Republic;</unparsedAffiliation></affiliation><affiliation xml:id="a2" countryCode="CZ"><unparsedAffiliation>University of South Bohemia, Faculty of Agriculture, Ceske Budejovice, Czech Republic;</unparsedAffiliation></affiliation><affiliation xml:id="a3" countryCode="FR"><unparsedAffiliation>Centre National de Recherche Scientifique, Station marine, Villefranche sur Mer, France</unparsedAffiliation></affiliation></affiliationGroup><abstractGroup><abstract type="main" xml:lang="en"><title type="main">Summary</title><p>A small urinary bladder attached to the seminal duct in caudal part of the abdominal cavity was registered for the first time in dissected males of tench. The urinary bladder wall was of whitish color and the bladder contained 0.5–2 ml of urine. When collected in the experiment, the tench sperm was white‐colored. Spermatozoa density is highly variable due to contamination by urine, and the latter additionally activates spontaneous motility of the spermatozoa. Seminal fluid contains ions such as Na<sup>+</sup> (18.4 ± 1.3 m<sc>m</sc>), K<sup>+</sup> (1.9 ± 0.6 m<sc>m</sc>), Ca<sup>2+</sup> (0.6 ± 0.2 m<sc>m</sc>) and Mg<sup>2+</sup> (0.5 ± 0.1 m<sc>m</sc>), leading to osmolality of 230 ± 82 mOsmol kg<sup>−1</sup> depending on the dilution by urine. Urea was detected in urine samples uncontaminated by sperm with an osmolality of 85 ± 58 mOsmol kg<sup>−1</sup>. Urine also contained high concentrations of ions such as Na<sup>+</sup> (30.9 ± 8.9 m<sc>m</sc>), K<sup>+</sup> (4.3 ± 2.9 m<sc>m</sc>), Ca<sup>2+</sup> (0.9 ± 0.5 m<sc>m</sc>) and Mg<sup>2+</sup> (0.6 ± 0.2 m<sc>m</sc>). The spontaneous sperm activation by urine was up to 100%, but could be prevented by collection in an immobilizing solution. Motility was observed for 90–100% spermatozoa just after their transfer to distilled water or in a swimming medium (SM, 30–45 m<sc>m</sc> KCl) with a velocity of 120–140 <i>μ</i>m s<sup>−1</sup>. A flagellar beat frequency of 60–70 Hz and forward motility lasted up to 80 s in distilled water, and up to 180 s in SM at room temperature.</p></abstract></abstractGroup></contentMeta></header></component></istex:document></istex:metadataXml><mods version="3.6"><titleInfo lang="en"><title>Urinary bladder, ionic composition of seminal fluid and urine with characterization of sperm motility in tench (Tinca tinca L.)</title></titleInfo><titleInfo type="abbreviated" lang="en"><title>Characterization of sperm motility in tench (Tinca tinca L.)</title></titleInfo><titleInfo type="alternative" contentType="CDATA" lang="en"><title>Urinary bladder, ionic composition of seminal fluid and urine with characterization of sperm motility in tench (Tinca tinca L.)</title></titleInfo><name type="personal"><namePart type="given">O.</namePart><namePart type="family">Linhart</namePart><affiliation>Joint Laboratory of Genetics, Physiology and Reproduction of Fish, Institute of Animal Physiology and Genetics, Academy of Sciences of the Czech Republic, University of South Bohemia, Research Institute of Fish Culture and Hydrobiology, Vodnany, Czech Republic;</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">M.</namePart><namePart type="family">Rodina</namePart><affiliation>Joint Laboratory of Genetics, Physiology and Reproduction of Fish, Institute of Animal Physiology and Genetics, Academy of Sciences of the Czech Republic, University of South Bohemia, Research Institute of Fish Culture and Hydrobiology, Vodnany, Czech Republic;</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">J.</namePart><namePart type="family">Bastl</namePart><affiliation>University of South Bohemia, Faculty of Agriculture, Ceske Budejovice, Czech Republic;</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">J.</namePart><namePart type="family">Cosson</namePart><affiliation>Centre National de Recherche Scientifique, Station marine, Villefranche sur Mer, France</affiliation><role><roleTerm type="text">author</roleTerm></role></name><typeOfResource>text</typeOfResource><genre type="article" displayLabel="article"></genre><originInfo><publisher>Blackwell Verlag GmbH</publisher><place><placeTerm type="text">Oxford, UK</placeTerm></place><dateIssued encoding="w3cdtf">2003-06</dateIssued><edition>Received: January 30, 2003 Accepted: March 1, 2003</edition><copyrightDate encoding="w3cdtf">2003</copyrightDate></originInfo><language><languageTerm type="code" authority="rfc3066">en</languageTerm><languageTerm type="code" authority="iso639-2b">eng</languageTerm></language><physicalDescription><internetMediaType>text/html</internetMediaType><extent unit="figures">5</extent></physicalDescription><abstract lang="en">A small urinary bladder attached to the seminal duct in caudal part of the abdominal cavity was registered for the first time in dissected males of tench. The urinary bladder wall was of whitish color and the bladder contained 0.5–2 ml of urine. When collected in the experiment, the tench sperm was white‐colored. Spermatozoa density is highly variable due to contamination by urine, and the latter additionally activates spontaneous motility of the spermatozoa. Seminal fluid contains ions such as Na+ (18.4 ± 1.3 mm), K+ (1.9 ± 0.6 mm), Ca2+ (0.6 ± 0.2 mm) and Mg2+ (0.5 ± 0.1 mm), leading to osmolality of 230 ± 82 mOsmol kg−1 depending on the dilution by urine. Urea was detected in urine samples uncontaminated by sperm with an osmolality of 85 ± 58 mOsmol kg−1. Urine also contained high concentrations of ions such as Na+ (30.9 ± 8.9 mm), K+ (4.3 ± 2.9 mm), Ca2+ (0.9 ± 0.5 mm) and Mg2+ (0.6 ± 0.2 mm). The spontaneous sperm activation by urine was up to 100%, but could be prevented by collection in an immobilizing solution. Motility was observed for 90–100% spermatozoa just after their transfer to distilled water or in a swimming medium (SM, 30–45 mm KCl) with a velocity of 120–140 μm s−1. A flagellar beat frequency of 60–70 Hz and forward motility lasted up to 80 s in distilled water, and up to 180 s in SM at room temperature.</abstract><relatedItem type="host"><titleInfo><title>Journal of Applied Ichthyology</title></titleInfo><genre type="journal">journal</genre><identifier type="ISSN">0175-8659</identifier><identifier type="eISSN">1439-0426</identifier><identifier type="DOI">10.1111/(ISSN)1439-0426</identifier><identifier type="PublisherID">JAI</identifier><part><date>2003</date><detail type="volume"><caption>vol.</caption><number>19</number></detail><detail type="issue"><caption>no.</caption><number>3</number></detail><extent unit="pages"><start>177</start><end>181</end><total>5</total></extent></part></relatedItem><identifier type="istex">C9911121349381FC117BD78A5FCE3BBA50E8582F</identifier><identifier type="DOI">10.1046/j.1439-0426.2003.00470.x</identifier><identifier type="ArticleID">JAI470</identifier><recordInfo><recordContentSource>WILEY</recordContentSource><recordOrigin>Blackwell Verlag GmbH</recordOrigin></recordInfo></mods></metadata><serie></serie></istex></record>Pour manipuler ce document sous Unix (Dilib)
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