Polymerase chain reaction amplification of the thymidine kinase and protein kinase-related genes of channel catfish virus and a putative pilchard herpesvirus
Identifieur interne : 000719 ( Istex/Corpus ); précédent : 000718; suivant : 000720Polymerase chain reaction amplification of the thymidine kinase and protein kinase-related genes of channel catfish virus and a putative pilchard herpesvirus
Auteurs : K. M. Tham ; C. D. MoonSource :
- Journal of Virological Methods [ 0166-0934 ] ; 1996.
Abstract
Polymerase chain reaction (PCR) amplification assays were developed for the detection of thymidine kinase (TK) and protein kinase (PK)-related genes of the channel catfish virus (CCV). Two pairs of primers were constructed based on the published nucleotide sequences of CCV and were used to amplify the expected fragments of 584 and 755 bp for the TK and PK-related genes, respectively. The amplified fragments were shown to be specific for each of the target genes by chemiluminescence Southern blot hybridisation with a digoxigenin-labelled 20-base internal probe. The optimised CCV PCR assay can be used to amplify TK- and PK-related genes in other mammalian and avian herpesviruses. The CCV TK PCR assay also amplified a TK-like gene in some pilchard's gills and tissues obtained in the recent epizootic of pilchard kills in New Zealand.
Url:
DOI: 10.1016/0166-0934(96)02070-8
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<record><TEI wicri:istexFullTextTei="biblStruct"><teiHeader><fileDesc><titleStmt><title>Polymerase chain reaction amplification of the thymidine kinase and protein kinase-related genes of channel catfish virus and a putative pilchard herpesvirus</title><author><name sortKey="Tham, K M" sort="Tham, K M" uniqKey="Tham K" first="K. M." last="Tham">K. M. Tham</name><affiliation><mods:affiliation>Virology Section, Central Animal Health Laboratory, MAF Quality Management, P.O. Box 40-063, Upper Hutt, New Zealand</mods:affiliation></affiliation></author><author><name sortKey="Moon, C D" sort="Moon, C D" uniqKey="Moon C" first="C. D." last="Moon">C. D. Moon</name><affiliation><mods:affiliation>Virology Section, Central Animal Health Laboratory, MAF Quality Management, P.O. Box 40-063, Upper Hutt, New Zealand</mods:affiliation></affiliation></author></titleStmt><publicationStmt><idno type="wicri:source">ISTEX</idno><idno type="RBID">ISTEX:A77171C7A971B02A831582BFA98E2244FBF692A9</idno><date when="1996" year="1996">1996</date><idno type="doi">10.1016/0166-0934(96)02070-8</idno><idno type="url">https://api.istex.fr/document/A77171C7A971B02A831582BFA98E2244FBF692A9/fulltext/pdf</idno><idno type="wicri:Area/Istex/Corpus">000719</idno><idno type="wicri:explorRef" wicri:stream="Istex" wicri:step="Corpus" wicri:corpus="ISTEX">000719</idno></publicationStmt><sourceDesc><biblStruct><analytic><title level="a">Polymerase chain reaction amplification of the thymidine kinase and protein kinase-related genes of channel catfish virus and a putative pilchard herpesvirus</title><author><name sortKey="Tham, K M" sort="Tham, K M" uniqKey="Tham K" first="K. M." last="Tham">K. M. Tham</name><affiliation><mods:affiliation>Virology Section, Central Animal Health Laboratory, MAF Quality Management, P.O. Box 40-063, Upper Hutt, New Zealand</mods:affiliation></affiliation></author><author><name sortKey="Moon, C D" sort="Moon, C D" uniqKey="Moon C" first="C. D." last="Moon">C. D. Moon</name><affiliation><mods:affiliation>Virology Section, Central Animal Health Laboratory, MAF Quality Management, P.O. Box 40-063, Upper Hutt, New Zealand</mods:affiliation></affiliation></author></analytic><monogr></monogr><series><title level="j">Journal of Virological Methods</title><title level="j" type="abbrev">VIRMET</title><idno type="ISSN">0166-0934</idno><imprint><publisher>ELSEVIER</publisher><date type="published" when="1996">1996</date><biblScope unit="volume">61</biblScope><biblScope unit="issue">1–2</biblScope><biblScope unit="page" from="65">65</biblScope><biblScope unit="page" to="72">72</biblScope></imprint><idno type="ISSN">0166-0934</idno></series><idno type="istex">A77171C7A971B02A831582BFA98E2244FBF692A9</idno><idno type="DOI">10.1016/0166-0934(96)02070-8</idno><idno type="PII">0166-0934(96)02070-8</idno></biblStruct></sourceDesc><seriesStmt><idno type="ISSN">0166-0934</idno></seriesStmt></fileDesc><profileDesc><textClass></textClass><langUsage><language ident="en">en</language></langUsage></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Polymerase chain reaction (PCR) amplification assays were developed for the detection of thymidine kinase (TK) and protein kinase (PK)-related genes of the channel catfish virus (CCV). Two pairs of primers were constructed based on the published nucleotide sequences of CCV and were used to amplify the expected fragments of 584 and 755 bp for the TK and PK-related genes, respectively. The amplified fragments were shown to be specific for each of the target genes by chemiluminescence Southern blot hybridisation with a digoxigenin-labelled 20-base internal probe. The optimised CCV PCR assay can be used to amplify TK- and PK-related genes in other mammalian and avian herpesviruses. The CCV TK PCR assay also amplified a TK-like gene in some pilchard's gills and tissues obtained in the recent epizootic of pilchard kills in New Zealand.</div></front></TEI><istex><corpusName>elsevier</corpusName><author><json:item><name>K.M. Tham</name><affiliations><json:string>Virology Section, Central Animal Health Laboratory, MAF Quality Management, P.O. Box 40-063, Upper Hutt, New Zealand</json:string></affiliations></json:item><json:item><name>C.D. Moon</name><affiliations><json:string>Virology Section, Central Animal Health Laboratory, MAF Quality Management, P.O. Box 40-063, Upper Hutt, New Zealand</json:string></affiliations></json:item></author><subject><json:item><lang><json:string>eng</json:string></lang><value>Thymine kinase</value></json:item><json:item><lang><json:string>eng</json:string></lang><value>Catfish virus</value></json:item><json:item><lang><json:string>eng</json:string></lang><value>Herpesvirus</value></json:item><json:item><lang><json:string>eng</json:string></lang><value>Pilchard</value></json:item></subject><language><json:string>eng</json:string></language><originalGenre><json:string>Full-length article</json:string></originalGenre><abstract>Polymerase chain reaction (PCR) amplification assays were developed for the detection of thymidine kinase (TK) and protein kinase (PK)-related genes of the channel catfish virus (CCV). Two pairs of primers were constructed based on the published nucleotide sequences of CCV and were used to amplify the expected fragments of 584 and 755 bp for the TK and PK-related genes, respectively. The amplified fragments were shown to be specific for each of the target genes by chemiluminescence Southern blot hybridisation with a digoxigenin-labelled 20-base internal probe. The optimised CCV PCR assay can be used to amplify TK- and PK-related genes in other mammalian and avian herpesviruses. 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Tel.: + 64 4 5281334; fax: + 64 4 5281376.</p></note><affiliation>Virology Section, Central Animal Health Laboratory, MAF Quality Management, P.O. Box 40-063, Upper Hutt, New Zealand</affiliation></author><author xml:id="author-2"><persName><forename type="first">C.D.</forename><surname>Moon</surname></persName><note type="biography">Present address: School of Biological Sciences, Massey University, New Zealand.</note><affiliation>Present address: School of Biological Sciences, Massey University, New Zealand.</affiliation><affiliation>Virology Section, Central Animal Health Laboratory, MAF Quality Management, P.O. Box 40-063, Upper Hutt, New Zealand</affiliation></author></analytic><monogr><title level="j">Journal of Virological Methods</title><title level="j" type="abbrev">VIRMET</title><idno type="pISSN">0166-0934</idno><idno type="PII">S0166-0934(00)X0016-X</idno><imprint><publisher>ELSEVIER</publisher><date type="published" when="1996"></date><biblScope unit="volume">61</biblScope><biblScope unit="issue">1–2</biblScope><biblScope unit="page" from="65">65</biblScope><biblScope unit="page" to="72">72</biblScope></imprint></monogr><idno type="istex">A77171C7A971B02A831582BFA98E2244FBF692A9</idno><idno type="DOI">10.1016/0166-0934(96)02070-8</idno><idno type="PII">0166-0934(96)02070-8</idno></biblStruct></sourceDesc></fileDesc><profileDesc><creation><date>1996</date></creation><langUsage><language ident="en">en</language></langUsage><abstract xml:lang="en"><p>Polymerase chain reaction (PCR) amplification assays were developed for the detection of thymidine kinase (TK) and protein kinase (PK)-related genes of the channel catfish virus (CCV). Two pairs of primers were constructed based on the published nucleotide sequences of CCV and were used to amplify the expected fragments of 584 and 755 bp for the TK and PK-related genes, respectively. The amplified fragments were shown to be specific for each of the target genes by chemiluminescence Southern blot hybridisation with a digoxigenin-labelled 20-base internal probe. The optimised CCV PCR assay can be used to amplify TK- and PK-related genes in other mammalian and avian herpesviruses. The CCV TK PCR assay also amplified a TK-like gene in some pilchard's gills and tissues obtained in the recent epizootic of pilchard kills in New Zealand.</p></abstract><textClass><keywords scheme="keyword"><list><head>Keywords</head><item><term>Thymine kinase</term></item><item><term>Catfish virus</term></item><item><term>Herpesvirus</term></item><item><term>Pilchard</term></item></list></keywords></textClass></profileDesc><revisionDesc><change when="1996">Published</change></revisionDesc></teiHeader></istex:fulltextTEI><json:item><extension>txt</extension><original>false</original><mimetype>text/plain</mimetype><uri>https://api.istex.fr/document/A77171C7A971B02A831582BFA98E2244FBF692A9/fulltext/txt</uri></json:item></fulltext><metadata><istex:metadataXml wicri:clean="Elsevier, elements deleted: tail"><istex:xmlDeclaration>version="1.0" encoding="utf-8"</istex:xmlDeclaration><istex:docType PUBLIC="-//ES//DTD journal article DTD version 4.5.2//EN//XML" URI="art452.dtd" name="istex:docType"></istex:docType><istex:document><converted-article version="4.5.2" docsubtype="fla"><item-info><jid>VIRMET</jid><aid>96020708</aid><ce:pii>0166-0934(96)02070-8</ce:pii><ce:doi>10.1016/0166-0934(96)02070-8</ce:doi><ce:copyright type="unknown" year="1996"></ce:copyright></item-info><head><ce:dochead><ce:textfn>Research paper</ce:textfn></ce:dochead><ce:title>Polymerase chain reaction amplification of the thymidine kinase and protein kinase-related genes of channel catfish virus and a putative pilchard herpesvirus</ce:title><ce:author-group><ce:author><ce:given-name>K.M.</ce:given-name><ce:surname>Tham</ce:surname><ce:cross-ref refid="COR1"><ce:sup>∗</ce:sup></ce:cross-ref></ce:author><ce:author><ce:given-name>C.D.</ce:given-name><ce:surname>Moon</ce:surname><ce:cross-ref refid="FN1"><ce:sup>1</ce:sup></ce:cross-ref></ce:author><ce:affiliation><ce:textfn>Virology Section, Central Animal Health Laboratory, MAF Quality Management, P.O. Box 40-063, Upper Hutt, New Zealand</ce:textfn></ce:affiliation><ce:correspondence id="COR1"><ce:label>∗</ce:label><ce:text>Corresponding author. Tel.: + 64 4 5281334; fax: + 64 4 5281376.</ce:text></ce:correspondence><ce:footnote id="FN1"><ce:label>1</ce:label><ce:note-para>Present address: School of Biological Sciences, Massey University, New Zealand.</ce:note-para></ce:footnote></ce:author-group><ce:date-accepted day="17" month="4" year="1996"></ce:date-accepted><ce:abstract><ce:section-title>Abstract</ce:section-title><ce:abstract-sec><ce:simple-para>Polymerase chain reaction (PCR) amplification assays were developed for the detection of thymidine kinase (TK) and protein kinase (PK)-related genes of the channel catfish virus (CCV). Two pairs of primers were constructed based on the published nucleotide sequences of CCV and were used to amplify the expected fragments of 584 and 755 bp for the TK and PK-related genes, respectively. The amplified fragments were shown to be specific for each of the target genes by chemiluminescence Southern blot hybridisation with a digoxigenin-labelled 20-base internal probe. The optimised CCV PCR assay can be used to amplify TK- and PK-related genes in other mammalian and avian herpesviruses. The CCV TK PCR assay also amplified a TK-like gene in some pilchard's gills and tissues obtained in the recent epizootic of pilchard kills in New Zealand.</ce:simple-para></ce:abstract-sec></ce:abstract><ce:keywords><ce:section-title>Keywords</ce:section-title><ce:keyword><ce:text>Thymine kinase</ce:text></ce:keyword><ce:keyword><ce:text>Catfish virus</ce:text></ce:keyword><ce:keyword><ce:text>Herpesvirus</ce:text></ce:keyword><ce:keyword><ce:text>Pilchard</ce:text></ce:keyword></ce:keywords></head></converted-article></istex:document></istex:metadataXml><mods version="3.6"><titleInfo><title>Polymerase chain reaction amplification of the thymidine kinase and protein kinase-related genes of channel catfish virus and a putative pilchard herpesvirus</title></titleInfo><titleInfo type="alternative" contentType="CDATA"><title>Polymerase chain reaction amplification of the thymidine kinase and protein kinase-related genes of channel catfish virus and a putative pilchard herpesvirus</title></titleInfo><name type="personal"><namePart type="given">K.M.</namePart><namePart type="family">Tham</namePart><affiliation>Virology Section, Central Animal Health Laboratory, MAF Quality Management, P.O. Box 40-063, Upper Hutt, New Zealand</affiliation><description>Corresponding author. Tel.: + 64 4 5281334; fax: + 64 4 5281376.</description><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">C.D.</namePart><namePart type="family">Moon</namePart><affiliation>Virology Section, Central Animal Health Laboratory, MAF Quality Management, P.O. Box 40-063, Upper Hutt, New Zealand</affiliation><description>Present address: School of Biological Sciences, Massey University, New Zealand.</description><role><roleTerm type="text">author</roleTerm></role></name><typeOfResource>text</typeOfResource><genre type="research-article" displayLabel="Full-length article"></genre><originInfo><publisher>ELSEVIER</publisher><dateIssued encoding="w3cdtf">1996</dateIssued><copyrightDate encoding="w3cdtf">1996</copyrightDate></originInfo><language><languageTerm type="code" authority="iso639-2b">eng</languageTerm><languageTerm type="code" authority="rfc3066">en</languageTerm></language><physicalDescription><internetMediaType>text/html</internetMediaType></physicalDescription><abstract lang="en">Polymerase chain reaction (PCR) amplification assays were developed for the detection of thymidine kinase (TK) and protein kinase (PK)-related genes of the channel catfish virus (CCV). Two pairs of primers were constructed based on the published nucleotide sequences of CCV and were used to amplify the expected fragments of 584 and 755 bp for the TK and PK-related genes, respectively. The amplified fragments were shown to be specific for each of the target genes by chemiluminescence Southern blot hybridisation with a digoxigenin-labelled 20-base internal probe. The optimised CCV PCR assay can be used to amplify TK- and PK-related genes in other mammalian and avian herpesviruses. The CCV TK PCR assay also amplified a TK-like gene in some pilchard's gills and tissues obtained in the recent epizootic of pilchard kills in New Zealand.</abstract><note type="content">Section title: Research paper</note><subject><genre>Keywords</genre><topic>Thymine kinase</topic><topic>Catfish virus</topic><topic>Herpesvirus</topic><topic>Pilchard</topic></subject><relatedItem type="host"><titleInfo><title>Journal of Virological Methods</title></titleInfo><titleInfo type="abbreviated"><title>VIRMET</title></titleInfo><genre type="journal">journal</genre><originInfo><dateIssued encoding="w3cdtf">199609</dateIssued></originInfo><identifier type="ISSN">0166-0934</identifier><identifier type="PII">S0166-0934(00)X0016-X</identifier><part><date>199609</date><detail type="volume"><number>61</number><caption>vol.</caption></detail><detail type="issue"><number>1–2</number><caption>no.</caption></detail><extent unit="issue pages"><start>1</start><end>163</end></extent><extent unit="pages"><start>65</start><end>72</end></extent></part></relatedItem><identifier type="istex">A77171C7A971B02A831582BFA98E2244FBF692A9</identifier><identifier type="DOI">10.1016/0166-0934(96)02070-8</identifier><identifier type="PII">0166-0934(96)02070-8</identifier><recordInfo><recordContentSource>ELSEVIER</recordContentSource></recordInfo></mods></metadata></istex></record>Pour manipuler ce document sous Unix (Dilib)
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