ISOLATION AND CHARACTERIZATION OF GONADOTROPIN ISOHORMONES FROM THE PITUITARY GLAND OF PIKE EEL (MURAENESOX CINEREUS)
Identifieur interne : 001422 ( Istex/Checkpoint ); précédent : 001421; suivant : 001423ISOLATION AND CHARACTERIZATION OF GONADOTROPIN ISOHORMONES FROM THE PITUITARY GLAND OF PIKE EEL (MURAENESOX CINEREUS)
Auteurs : Fore-Lien Huang [Taïwan] ; Chang-Jen Huang [Taïwan] ; Sue-Hwa Lin [Taïwan] ; Tung-Bin Lo [Taïwan] ; Harold Papkoff [États-Unis]Source :
- International Journal of Peptide and Protein Research [ 0367-8377 ] ; 1981-07.
English descriptors
Abstract
Pike eel gonadotropins were isolated from pituitary glands by 40% alcohol‐6% ammonium acetate, pH 5.1 extraction and were purified by DEAE‐cellulose chromatography and electrophoresis into four electrophoretically homogeneous forms. These four isohormones were biologically identified as gonadotropins by the stimulation of 32P‐uptake in 1‐day‐old chick testes, by the induction of ovulation in catfish, and by the in vitro production of testosterone from isolated rat Leydig cells and of androgen from carp testes. The amino acid composition of the isohormones were similar to other known piscine gonadotropins (carp and salmon) and were composed of two non‐identical subunits with Tyr and Ser as N‐terminal amino acid residues. The molecular weights of two subunits were 15 000 and 10 500, respectively, as estimated by SDS‐gel disc electrophoresis.
Url:
DOI: 10.1111/j.1399-3011.1981.tb02041.x
Affiliations:
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level="j">International Journal of Peptide and Protein Research</title><idno type="ISSN">0367-8377</idno><idno type="eISSN">1399-3011</idno><imprint><publisher>Blackwell Publishing Ltd</publisher><pubPlace>Oxford, UK</pubPlace><date type="published" when="1981-07">1981-07</date><biblScope unit="volume">18</biblScope><biblScope unit="issue">1</biblScope><biblScope unit="page" from="69">69</biblScope><biblScope unit="page" to="78">78</biblScope></imprint><idno type="ISSN">0367-8377</idno></series><idno type="istex">99B1E16277E87994B7906F3E7E81D084C794736B</idno><idno type="DOI">10.1111/j.1399-3011.1981.tb02041.x</idno><idno type="ArticleID">CBDD69</idno></biblStruct></sourceDesc><seriesStmt><idno type="ISSN">0367-8377</idno></seriesStmt></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Muraenesox cinereus pike eel</term><term>gonadotropin</term><term>isohormones</term><term>pituitary</term></keywords></textClass><langUsage><language ident="en">en</language></langUsage></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Pike eel gonadotropins were isolated from pituitary glands by 40% alcohol‐6% ammonium acetate, pH 5.1 extraction and were purified by DEAE‐cellulose chromatography and electrophoresis into four electrophoretically homogeneous forms. These four isohormones were biologically identified as gonadotropins by the stimulation of 32P‐uptake in 1‐day‐old chick testes, by the induction of ovulation in catfish, and by the in vitro production of testosterone from isolated rat Leydig cells and of androgen from carp testes. The amino acid composition of the isohormones were similar to other known piscine gonadotropins (carp and salmon) and were composed of two non‐identical subunits with Tyr and Ser as N‐terminal amino acid residues. 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