ISOLATION AND CHARACTERIZATION OF GONADOTROPIN ISOHORMONES FROM THE PITUITARY GLAND OF PIKE EEL (MURAENESOX CINEREUS)
Identifieur interne : 000462 ( Istex/Corpus ); précédent : 000461; suivant : 000463ISOLATION AND CHARACTERIZATION OF GONADOTROPIN ISOHORMONES FROM THE PITUITARY GLAND OF PIKE EEL (MURAENESOX CINEREUS)
Auteurs : Fore-Lien Huang ; Chang-Jen Huang ; Sue-Hwa Lin ; Tung-Bin Lo ; Harold PapkoffSource :
- International Journal of Peptide and Protein Research [ 0367-8377 ] ; 1981-07.
English descriptors
Abstract
Pike eel gonadotropins were isolated from pituitary glands by 40% alcohol‐6% ammonium acetate, pH 5.1 extraction and were purified by DEAE‐cellulose chromatography and electrophoresis into four electrophoretically homogeneous forms. These four isohormones were biologically identified as gonadotropins by the stimulation of 32P‐uptake in 1‐day‐old chick testes, by the induction of ovulation in catfish, and by the in vitro production of testosterone from isolated rat Leydig cells and of androgen from carp testes. The amino acid composition of the isohormones were similar to other known piscine gonadotropins (carp and salmon) and were composed of two non‐identical subunits with Tyr and Ser as N‐terminal amino acid residues. The molecular weights of two subunits were 15 000 and 10 500, respectively, as estimated by SDS‐gel disc electrophoresis.
Url:
DOI: 10.1111/j.1399-3011.1981.tb02041.x
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ISTEX:99B1E16277E87994B7906F3E7E81D084C794736BLe document en format XML
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Box 23–106 Tapei Taiwan</mods:affiliation></affiliation></author><author><name sortKey="Papkoff, Harold" sort="Papkoff, Harold" uniqKey="Papkoff H" first="Harold" last="Papkoff">Harold Papkoff</name><affiliation><mods:affiliation>Hormone Research Laboratory, University of California, San Francisco, California, USA</mods:affiliation></affiliation></author></analytic><monogr></monogr><series><title level="j">International Journal of Peptide and Protein Research</title><idno type="ISSN">0367-8377</idno><idno type="eISSN">1399-3011</idno><imprint><publisher>Blackwell Publishing Ltd</publisher><pubPlace>Oxford, UK</pubPlace><date type="published" when="1981-07">1981-07</date><biblScope unit="volume">18</biblScope><biblScope unit="issue">1</biblScope><biblScope unit="page" from="69">69</biblScope><biblScope unit="page" to="78">78</biblScope></imprint><idno type="ISSN">0367-8377</idno></series><idno type="istex">99B1E16277E87994B7906F3E7E81D084C794736B</idno><idno type="DOI">10.1111/j.1399-3011.1981.tb02041.x</idno><idno type="ArticleID">CBDD69</idno></biblStruct></sourceDesc><seriesStmt><idno type="ISSN">0367-8377</idno></seriesStmt></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Muraenesox cinereus pike eel</term><term>gonadotropin</term><term>isohormones</term><term>pituitary</term></keywords></textClass><langUsage><language ident="en">en</language></langUsage></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Pike eel gonadotropins were isolated from pituitary glands by 40% alcohol‐6% ammonium acetate, pH 5.1 extraction and were purified by DEAE‐cellulose chromatography and electrophoresis into four electrophoretically homogeneous forms. These four isohormones were biologically identified as gonadotropins by the stimulation of 32P‐uptake in 1‐day‐old chick testes, by the induction of ovulation in catfish, and by the in vitro production of testosterone from isolated rat Leydig cells and of androgen from carp testes. The amino acid composition of the isohormones were similar to other known piscine gonadotropins (carp and salmon) and were composed of two non‐identical subunits with Tyr and Ser as N‐terminal amino acid residues. The molecular weights of two subunits were 15 000 and 10 500, respectively, as estimated by SDS‐gel disc electrophoresis.</div></front></TEI><istex><corpusName>wiley</corpusName><author><json:item><name>FORE‐LIEN HUANG</name><affiliations><json:string>Institute of Biological Chemistry, Academia Sinica and Institute of Biochemical Sciences, National Taiwan University, Taipei, Taiwan</json:string></affiliations></json:item><json:item><name>CHANG‐JEN HUANG</name><affiliations><json:string>Institute of Biological Chemistry, Academia Sinica and Institute of Biochemical Sciences, National Taiwan University, Taipei, Taiwan</json:string></affiliations></json:item><json:item><name>SUE‐HWA LIN</name><affiliations><json:string>Institute of Biological Chemistry, Academia Sinica and Institute of Biochemical Sciences, National Taiwan University, Taipei, Taiwan</json:string></affiliations></json:item><json:item><name>TUNG‐BIN LO</name><affiliations><json:string>Institute of Biological Chemistry, Academia Sinica and Institute of Biochemical Sciences, National Taiwan University, Taipei, Taiwan</json:string><json:string>Institute of Biological Chemistry Academica Sinica P.O. Box 23–106 Tapei Taiwan</json:string></affiliations></json:item><json:item><name>HAROLD PAPKOFF</name><affiliations><json:string>Hormone Research Laboratory, University of California, San Francisco, California, USA</json:string></affiliations></json:item></author><subject><json:item><lang><json:string>eng</json:string></lang><value>gonadotropin</value></json:item><json:item><lang><json:string>eng</json:string></lang><value>isohormones</value></json:item><json:item><lang><json:string>eng</json:string></lang><value>Muraenesox cinereus pike eel</value></json:item><json:item><lang><json:string>eng</json:string></lang><value>pituitary</value></json:item></subject><articleId><json:string>CBDD69</json:string></articleId><language><json:string>eng</json:string></language><originalGenre><json:string>article</json:string></originalGenre><abstract>Pike eel gonadotropins were isolated from pituitary glands by 40% alcohol‐6% ammonium acetate, pH 5.1 extraction and were purified by DEAE‐cellulose chromatography and electrophoresis into four electrophoretically homogeneous forms. These four isohormones were biologically identified as gonadotropins by the stimulation of 32P‐uptake in 1‐day‐old chick testes, by the induction of ovulation in catfish, and by the in vitro production of testosterone from isolated rat Leydig cells and of androgen from carp testes. The amino acid composition of the isohormones were similar to other known piscine gonadotropins (carp and salmon) and were composed of two non‐identical subunits with Tyr and Ser as N‐terminal amino acid residues. 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Box 23–106 Tapei Taiwan</affiliation></author><author xml:id="author-5"><persName><forename type="first">HAROLD</forename><surname>PAPKOFF</surname></persName><affiliation>Hormone Research Laboratory, University of California, San Francisco, California, USA</affiliation></author></analytic><monogr><title level="j">International Journal of Peptide and Protein Research</title><idno type="pISSN">0367-8377</idno><idno type="eISSN">1399-3011</idno><idno type="DOI">10.1111/(ISSN)1399-3011a</idno><imprint><publisher>Blackwell Publishing Ltd</publisher><pubPlace>Oxford, UK</pubPlace><date type="published" when="1981-07"></date><biblScope unit="volume">18</biblScope><biblScope unit="issue">1</biblScope><biblScope unit="page" from="69">69</biblScope><biblScope unit="page" to="78">78</biblScope></imprint></monogr><idno type="istex">99B1E16277E87994B7906F3E7E81D084C794736B</idno><idno type="DOI">10.1111/j.1399-3011.1981.tb02041.x</idno><idno type="ArticleID">CBDD69</idno></biblStruct></sourceDesc></fileDesc><profileDesc><creation><date>1981</date></creation><langUsage><language ident="en">en</language></langUsage><abstract xml:lang="en"><p>Pike eel gonadotropins were isolated from pituitary glands by 40% alcohol‐6% ammonium acetate, pH 5.1 extraction and were purified by DEAE‐cellulose chromatography and electrophoresis into four electrophoretically homogeneous forms. 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Box 23–106 Tapei Taiwan</correspondenceTo><linkGroup><link type="toTypesetVersion" href="file:CBDD.CBDD69.pdf"></link></linkGroup></publicationMeta><contentMeta><unparsedEditorialHistory>Received 25 November 1980, accepted for publication 23 January 1981</unparsedEditorialHistory><countGroup><count type="referenceTotal" number="34"></count><count type="linksCrossRef" number="6"></count></countGroup><titleGroup><title type="main">ISOLATION AND CHARACTERIZATION OF GONADOTROPIN ISOHORMONES FROM THE PITUITARY GLAND OF PIKE EEL (<i>MURAENESOX CINEREUS</i>)</title></titleGroup><creators><creator creatorRole="author" xml:id="cr1" affiliationRef="#a1"><personName><givenNames>FORE‐LIEN</givenNames><familyName>HUANG</familyName></personName></creator><creator creatorRole="author" xml:id="cr2" affiliationRef="#a1"><personName><givenNames>CHANG‐JEN</givenNames><familyName>HUANG</familyName></personName></creator><creator creatorRole="author" xml:id="cr3" affiliationRef="#a1"><personName><givenNames>SUE‐HWA</givenNames><familyName>LIN</familyName></personName></creator><creator creatorRole="author" xml:id="cr4" affiliationRef="#a1" corresponding="yes"><personName><givenNames>TUNG‐BIN</givenNames><familyName>LO</familyName></personName></creator><creator creatorRole="author" xml:id="cr5" affiliationRef="#a2"><personName><givenNames>HAROLD</givenNames><familyName>PAPKOFF</familyName></personName></creator></creators><affiliationGroup><affiliation xml:id="a1"><unparsedAffiliation>Institute of Biological Chemistry, Academia Sinica and Institute of Biochemical Sciences, National Taiwan University, Taipei, Taiwan</unparsedAffiliation></affiliation><affiliation xml:id="a2" countryCode="US"><unparsedAffiliation>Hormone Research Laboratory, University of California, San Francisco, California, USA</unparsedAffiliation></affiliation></affiliationGroup><keywordGroup xml:lang="en"><keyword xml:id="k1">gonadotropin</keyword><keyword xml:id="k2">isohormones</keyword><keyword xml:id="k3"><i>Muraenesox cinereus</i> pike eel</keyword><keyword xml:id="k4">pituitary</keyword></keywordGroup><abstractGroup><abstract type="main" xml:lang="en"><p>Pike eel gonadotropins were isolated from pituitary glands by 40% alcohol‐6% ammonium acetate, pH 5.1 extraction and were purified by DEAE‐cellulose chromatography and electrophoresis into four electrophoretically homogeneous forms. 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The molecular weights of two subunits were 15 000 and 10 500, respectively, as estimated by SDS‐gel disc electrophoresis.</p></abstract></abstractGroup></contentMeta></header></component></istex:document></istex:metadataXml><mods version="3.6"><titleInfo lang="en"><title>ISOLATION AND CHARACTERIZATION OF GONADOTROPIN ISOHORMONES FROM THE PITUITARY GLAND OF PIKE EEL (MURAENESOX CINEREUS)</title></titleInfo><titleInfo type="alternative" contentType="CDATA" lang="en"><title>ISOLATION AND CHARACTERIZATION OF GONADOTROPIN ISOHORMONES FROM THE PITUITARY GLAND OF PIKE EEL (MURAENESOX CINEREUS)</title></titleInfo><name type="personal"><namePart type="given">FORE‐LIEN</namePart><namePart type="family">HUANG</namePart><affiliation>Institute of Biological Chemistry, Academia Sinica and Institute of Biochemical Sciences, National Taiwan University, Taipei, Taiwan</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">CHANG‐JEN</namePart><namePart type="family">HUANG</namePart><affiliation>Institute of Biological Chemistry, Academia Sinica and Institute of Biochemical Sciences, National Taiwan University, Taipei, Taiwan</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">SUE‐HWA</namePart><namePart type="family">LIN</namePart><affiliation>Institute of Biological Chemistry, Academia Sinica and Institute of Biochemical Sciences, National Taiwan University, Taipei, Taiwan</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">TUNG‐BIN</namePart><namePart type="family">LO</namePart><affiliation>Institute of Biological Chemistry, Academia Sinica and Institute of Biochemical Sciences, National Taiwan University, Taipei, Taiwan</affiliation><affiliation>Institute of Biological Chemistry Academica Sinica P.O. Box 23–106 Tapei Taiwan</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">HAROLD</namePart><namePart type="family">PAPKOFF</namePart><affiliation>Hormone Research Laboratory, University of California, San Francisco, California, USA</affiliation><role><roleTerm type="text">author</roleTerm></role></name><typeOfResource>text</typeOfResource><genre type="article" displayLabel="article"></genre><originInfo><publisher>Blackwell Publishing Ltd</publisher><place><placeTerm type="text">Oxford, UK</placeTerm></place><dateIssued encoding="w3cdtf">1981-07</dateIssued><edition>Received 25 November 1980, accepted for publication 23 January 1981</edition><copyrightDate encoding="w3cdtf">1981</copyrightDate></originInfo><language><languageTerm type="code" authority="rfc3066">en</languageTerm><languageTerm type="code" authority="iso639-2b">eng</languageTerm></language><physicalDescription><internetMediaType>text/html</internetMediaType><extent unit="references">34</extent></physicalDescription><abstract lang="en">Pike eel gonadotropins were isolated from pituitary glands by 40% alcohol‐6% ammonium acetate, pH 5.1 extraction and were purified by DEAE‐cellulose chromatography and electrophoresis into four electrophoretically homogeneous forms. These four isohormones were biologically identified as gonadotropins by the stimulation of 32P‐uptake in 1‐day‐old chick testes, by the induction of ovulation in catfish, and by the in vitro production of testosterone from isolated rat Leydig cells and of androgen from carp testes. The amino acid composition of the isohormones were similar to other known piscine gonadotropins (carp and salmon) and were composed of two non‐identical subunits with Tyr and Ser as N‐terminal amino acid residues. The molecular weights of two subunits were 15 000 and 10 500, respectively, as estimated by SDS‐gel disc electrophoresis.</abstract><subject lang="en"><genre>keywords</genre><topic>gonadotropin</topic><topic>isohormones</topic><topic>Muraenesox cinereus pike eel</topic><topic>pituitary</topic></subject><relatedItem type="host"><titleInfo><title>International Journal of Peptide and Protein Research</title></titleInfo><genre type="journal">journal</genre><identifier type="ISSN">0367-8377</identifier><identifier type="eISSN">1399-3011</identifier><identifier type="DOI">10.1111/(ISSN)1399-3011a</identifier><identifier type="PublisherID">CBDD</identifier><part><date>1981</date><detail type="volume"><caption>vol.</caption><number>18</number></detail><detail type="issue"><caption>no.</caption><number>1</number></detail><extent unit="pages"><start>69</start><end>78</end><total>10</total></extent></part></relatedItem><identifier type="istex">99B1E16277E87994B7906F3E7E81D084C794736B</identifier><identifier type="DOI">10.1111/j.1399-3011.1981.tb02041.x</identifier><identifier type="ArticleID">CBDD69</identifier><accessCondition type="use and reproduction" contentType="copyright">© 1981 Munksgaard, Copenhagen</accessCondition><recordInfo><recordContentSource>WILEY</recordContentSource><recordOrigin>Blackwell Publishing Ltd</recordOrigin></recordInfo></mods></metadata><serie></serie></istex></record>Pour manipuler ce document sous Unix (Dilib)
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|étape= Corpus
|type= RBID
|clé= ISTEX:99B1E16277E87994B7906F3E7E81D084C794736B
|texte= ISOLATION AND CHARACTERIZATION OF GONADOTROPIN ISOHORMONES FROM THE PITUITARY GLAND OF PIKE EEL (MURAENESOX CINEREUS)
}}
| This area was generated with Dilib version V0.6.27. |  |