Variability of the immunoglobulin light chain in the Siberian sturgeon, Acipenser baeri
Identifieur interne : 000D05 ( Istex/Checkpoint ); précédent : 000D04; suivant : 000D06Variability of the immunoglobulin light chain in the Siberian sturgeon, Acipenser baeri
Auteurs : Mats L. Lundqvist [Suède] ; Lars Pilström [Suède]Source :
- Developmental and Comparative Immunology [ 0145-305X ] ; 1999.
Abstract
All sturgeon VL segments isolated in this study belong to a single family, VLI, which can be divided into two subfamilies. Of the 79 cDNA clones isolated, 76 belong to the larger subfamily, VLIa, and only 3 clones constitute the smaller subfamily, VLIb. To evaluate variability, the Shannon entropy was estimated for each individual amino acid position, and to facilitate comparisons of variability between species the mean entropy of the CDR regions was calculated. In such a comparison, the sturgeon was found to have CDR1 and CDR3 variability approaching those found in mouse and clawed frog, but showed very low variability for CDR2. Amino acid position 50 does however display variability in the range of mouse and clawed frog. It is further confirmed that the sturgeon has numerous J segments, but that the junctional diversity does not contribute greatly to the diversity of the light chain. Comparisons of cDNA clones and a genomic VL segment indicate that the VL undergoes changes, particularly in the CDR regions, in a manner that can be explained by somatic hypermutation and/or gene conversion.
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DOI: 10.1016/S0145-305X(99)00049-X
Affiliations:
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Lundqvist</name><affiliation wicri:level="4"><country xml:lang="fr">Suède</country><wicri:regionArea>Department of Cell and Molecular Biology, Immunology Programme, BMC, Uppsala University, PO Box 596, S-751 24 Uppsala</wicri:regionArea><orgName type="university">Université d'Uppsala</orgName><placeName><settlement type="city">Uppsala</settlement><region nuts="1">Svealand</region><region nuts="1">East Middle Sweden</region></placeName></affiliation></author><author><name sortKey="Pilstrom, Lars" sort="Pilstrom, Lars" uniqKey="Pilstrom L" first="Lars" last="Pilström">Lars Pilström</name><affiliation wicri:level="4"><country xml:lang="fr">Suède</country><wicri:regionArea>Department of Cell and Molecular Biology, Immunology Programme, BMC, Uppsala University, PO Box 596, S-751 24 Uppsala</wicri:regionArea><orgName type="university">Université d'Uppsala</orgName><placeName><settlement type="city">Uppsala</settlement><region nuts="1">Svealand</region><region nuts="1">East Middle Sweden</region></placeName></affiliation><affiliation wicri:level="1"><country wicri:rule="url">Suède</country></affiliation></author></analytic><monogr></monogr><series><title level="j">Developmental and Comparative Immunology</title><title level="j" type="abbrev">DCI</title><idno type="ISSN">0145-305X</idno><imprint><publisher>ELSEVIER</publisher><date type="published" when="1999">1999</date><biblScope unit="volume">23</biblScope><biblScope unit="issue">7–8</biblScope><biblScope unit="page" from="607">607</biblScope><biblScope unit="page" to="615">615</biblScope></imprint><idno type="ISSN">0145-305X</idno></series><idno type="istex">1A8D510C3AFB1B631F2A6D43945472E90570DA63</idno><idno type="DOI">10.1016/S0145-305X(99)00049-X</idno><idno type="PII">S0145-305X(99)00049-X</idno></biblStruct></sourceDesc><seriesStmt><idno type="ISSN">0145-305X</idno></seriesStmt></fileDesc><profileDesc><textClass></textClass><langUsage><language ident="en">en</language></langUsage></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">All sturgeon VL segments isolated in this study belong to a single family, VLI, which can be divided into two subfamilies. Of the 79 cDNA clones isolated, 76 belong to the larger subfamily, VLIa, and only 3 clones constitute the smaller subfamily, VLIb. To evaluate variability, the Shannon entropy was estimated for each individual amino acid position, and to facilitate comparisons of variability between species the mean entropy of the CDR regions was calculated. In such a comparison, the sturgeon was found to have CDR1 and CDR3 variability approaching those found in mouse and clawed frog, but showed very low variability for CDR2. Amino acid position 50 does however display variability in the range of mouse and clawed frog. It is further confirmed that the sturgeon has numerous J segments, but that the junctional diversity does not contribute greatly to the diversity of the light chain. Comparisons of cDNA clones and a genomic VL segment indicate that the VL undergoes changes, particularly in the CDR regions, in a manner that can be explained by somatic hypermutation and/or gene conversion.</div></front></TEI><affiliations><list><country><li>Suède</li></country><region><li>East Middle Sweden</li><li>Svealand</li></region><settlement><li>Uppsala</li></settlement><orgName><li>Université d'Uppsala</li></orgName></list><tree><country name="Suède"><region name="Svealand"><name sortKey="Lundqvist, Mats L" sort="Lundqvist, Mats L" uniqKey="Lundqvist M" first="Mats L" last="Lundqvist">Mats L. Lundqvist</name></region><name sortKey="Pilstrom, Lars" sort="Pilstrom, Lars" uniqKey="Pilstrom L" first="Lars" last="Pilström">Lars Pilström</name><name sortKey="Pilstrom, Lars" sort="Pilstrom, Lars" uniqKey="Pilstrom L" first="Lars" last="Pilström">Lars Pilström</name></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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