Variability of the immunoglobulin light chain in the Siberian sturgeon, Acipenser baeri
Identifieur interne : 000B35 ( Istex/Corpus ); précédent : 000B34; suivant : 000B36Variability of the immunoglobulin light chain in the Siberian sturgeon, Acipenser baeri
Auteurs : Mats L. Lundqvist ; Lars PilströmSource :
- Developmental and Comparative Immunology [ 0145-305X ] ; 1999.
Abstract
All sturgeon VL segments isolated in this study belong to a single family, VLI, which can be divided into two subfamilies. Of the 79 cDNA clones isolated, 76 belong to the larger subfamily, VLIa, and only 3 clones constitute the smaller subfamily, VLIb. To evaluate variability, the Shannon entropy was estimated for each individual amino acid position, and to facilitate comparisons of variability between species the mean entropy of the CDR regions was calculated. In such a comparison, the sturgeon was found to have CDR1 and CDR3 variability approaching those found in mouse and clawed frog, but showed very low variability for CDR2. Amino acid position 50 does however display variability in the range of mouse and clawed frog. It is further confirmed that the sturgeon has numerous J segments, but that the junctional diversity does not contribute greatly to the diversity of the light chain. Comparisons of cDNA clones and a genomic VL segment indicate that the VL undergoes changes, particularly in the CDR regions, in a manner that can be explained by somatic hypermutation and/or gene conversion.
Url:
DOI: 10.1016/S0145-305X(99)00049-X
Links to Exploration step
ISTEX:1A8D510C3AFB1B631F2A6D43945472E90570DA63Le document en format XML
<record><TEI wicri:istexFullTextTei="biblStruct"><teiHeader><fileDesc><titleStmt><title>Variability of the immunoglobulin light chain in the Siberian sturgeon, Acipenser baeri</title><author><name sortKey="Lundqvist, Mats L" sort="Lundqvist, Mats L" uniqKey="Lundqvist M" first="Mats L" last="Lundqvist">Mats L. Lundqvist</name><affiliation><mods:affiliation>Department of Cell and Molecular Biology, Immunology Programme, BMC, Uppsala University, PO Box 596, S-751 24 Uppsala, Sweden</mods:affiliation></affiliation></author><author><name sortKey="Pilstrom, Lars" sort="Pilstrom, Lars" uniqKey="Pilstrom L" first="Lars" last="Pilström">Lars Pilström</name><affiliation><mods:affiliation>Department of Cell and Molecular Biology, Immunology Programme, BMC, Uppsala University, PO Box 596, S-751 24 Uppsala, Sweden</mods:affiliation></affiliation><affiliation><mods:affiliation>E-mail: Lars.Pilstrom@icm.uu.se</mods:affiliation></affiliation></author></titleStmt><publicationStmt><idno type="wicri:source">ISTEX</idno><idno type="RBID">ISTEX:1A8D510C3AFB1B631F2A6D43945472E90570DA63</idno><date when="1999" year="1999">1999</date><idno type="doi">10.1016/S0145-305X(99)00049-X</idno><idno type="url">https://api.istex.fr/document/1A8D510C3AFB1B631F2A6D43945472E90570DA63/fulltext/pdf</idno><idno type="wicri:Area/Istex/Corpus">000B35</idno><idno type="wicri:explorRef" wicri:stream="Istex" wicri:step="Corpus" wicri:corpus="ISTEX">000B35</idno></publicationStmt><sourceDesc><biblStruct><analytic><title level="a">Variability of the immunoglobulin light chain in the Siberian sturgeon, Acipenser baeri</title><author><name sortKey="Lundqvist, Mats L" sort="Lundqvist, Mats L" uniqKey="Lundqvist M" first="Mats L" last="Lundqvist">Mats L. Lundqvist</name><affiliation><mods:affiliation>Department of Cell and Molecular Biology, Immunology Programme, BMC, Uppsala University, PO Box 596, S-751 24 Uppsala, Sweden</mods:affiliation></affiliation></author><author><name sortKey="Pilstrom, Lars" sort="Pilstrom, Lars" uniqKey="Pilstrom L" first="Lars" last="Pilström">Lars Pilström</name><affiliation><mods:affiliation>Department of Cell and Molecular Biology, Immunology Programme, BMC, Uppsala University, PO Box 596, S-751 24 Uppsala, Sweden</mods:affiliation></affiliation><affiliation><mods:affiliation>E-mail: Lars.Pilstrom@icm.uu.se</mods:affiliation></affiliation></author></analytic><monogr></monogr><series><title level="j">Developmental and Comparative Immunology</title><title level="j" type="abbrev">DCI</title><idno type="ISSN">0145-305X</idno><imprint><publisher>ELSEVIER</publisher><date type="published" when="1999">1999</date><biblScope unit="volume">23</biblScope><biblScope unit="issue">7–8</biblScope><biblScope unit="page" from="607">607</biblScope><biblScope unit="page" to="615">615</biblScope></imprint><idno type="ISSN">0145-305X</idno></series><idno type="istex">1A8D510C3AFB1B631F2A6D43945472E90570DA63</idno><idno type="DOI">10.1016/S0145-305X(99)00049-X</idno><idno type="PII">S0145-305X(99)00049-X</idno></biblStruct></sourceDesc><seriesStmt><idno type="ISSN">0145-305X</idno></seriesStmt></fileDesc><profileDesc><textClass></textClass><langUsage><language ident="en">en</language></langUsage></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">All sturgeon VL segments isolated in this study belong to a single family, VLI, which can be divided into two subfamilies. Of the 79 cDNA clones isolated, 76 belong to the larger subfamily, VLIa, and only 3 clones constitute the smaller subfamily, VLIb. To evaluate variability, the Shannon entropy was estimated for each individual amino acid position, and to facilitate comparisons of variability between species the mean entropy of the CDR regions was calculated. In such a comparison, the sturgeon was found to have CDR1 and CDR3 variability approaching those found in mouse and clawed frog, but showed very low variability for CDR2. Amino acid position 50 does however display variability in the range of mouse and clawed frog. It is further confirmed that the sturgeon has numerous J segments, but that the junctional diversity does not contribute greatly to the diversity of the light chain. Comparisons of cDNA clones and a genomic VL segment indicate that the VL undergoes changes, particularly in the CDR regions, in a manner that can be explained by somatic hypermutation and/or gene conversion.</div></front></TEI><istex><corpusName>elsevier</corpusName><author><json:item><name>Mats L Lundqvist</name><affiliations><json:string>Department of Cell and Molecular Biology, Immunology Programme, BMC, Uppsala University, PO Box 596, S-751 24 Uppsala, Sweden</json:string></affiliations></json:item><json:item><name>Lars Pilström</name><affiliations><json:string>Department of Cell and Molecular Biology, Immunology Programme, BMC, Uppsala University, PO Box 596, S-751 24 Uppsala, Sweden</json:string><json:string>E-mail: Lars.Pilstrom@icm.uu.se</json:string></affiliations></json:item></author><subject><json:item><lang><json:string>eng</json:string></lang><value>Immunoglobulin</value></json:item><json:item><lang><json:string>eng</json:string></lang><value>Light chain</value></json:item><json:item><lang><json:string>eng</json:string></lang><value>VL gene</value></json:item><json:item><lang><json:string>eng</json:string></lang><value>Diversity</value></json:item><json:item><lang><json:string>eng</json:string></lang><value>Evolution</value></json:item><json:item><lang><json:string>eng</json:string></lang><value>Siberian sturgeon</value></json:item><json:item><lang><json:string>eng</json:string></lang><value>Acipenser baeri</value></json:item><json:item><lang><json:string>eng</json:string></lang><value>Ig, Immunoglobulin</value></json:item><json:item><lang><json:string>eng</json:string></lang><value>LP, Leader peptide</value></json:item><json:item><lang><json:string>eng</json:string></lang><value>V, Variable</value></json:item><json:item><lang><json:string>eng</json:string></lang><value>J, Joining</value></json:item><json:item><lang><json:string>eng</json:string></lang><value>CDR, Complementarity determining region</value></json:item><json:item><lang><json:string>eng</json:string></lang><value>FR, Framework</value></json:item><json:item><lang><json:string>eng</json:string></lang><value>RSS, Recombination signal sequence</value></json:item><json:item><lang><json:string>eng</json:string></lang><value>aa, Amino acid</value></json:item><json:item><lang><json:string>eng</json:string></lang><value>nt, Nucleotide(s)</value></json:item></subject><language><json:string>eng</json:string></language><originalGenre><json:string>Full-length article</json:string></originalGenre><abstract>All sturgeon VL segments isolated in this study belong to a single family, VLI, which can be divided into two subfamilies. Of the 79 cDNA clones isolated, 76 belong to the larger subfamily, VLIa, and only 3 clones constitute the smaller subfamily, VLIb. To evaluate variability, the Shannon entropy was estimated for each individual amino acid position, and to facilitate comparisons of variability between species the mean entropy of the CDR regions was calculated. In such a comparison, the sturgeon was found to have CDR1 and CDR3 variability approaching those found in mouse and clawed frog, but showed very low variability for CDR2. Amino acid position 50 does however display variability in the range of mouse and clawed frog. It is further confirmed that the sturgeon has numerous J segments, but that the junctional diversity does not contribute greatly to the diversity of the light chain. Comparisons of cDNA clones and a genomic VL segment indicate that the VL undergoes changes, particularly in the CDR regions, in a manner that can be explained by somatic hypermutation and/or gene conversion.</abstract><qualityIndicators><score>6.592</score><pdfVersion>1.2</pdfVersion><pdfPageSize>595 x 792 pts</pdfPageSize><refBibsNative>true</refBibsNative><keywordCount>16</keywordCount><abstractCharCount>1106</abstractCharCount><pdfWordCount>4444</pdfWordCount><pdfCharCount>25981</pdfCharCount><pdfPageCount>9</pdfPageCount><abstractWordCount>179</abstractWordCount></qualityIndicators><title>Variability of the immunoglobulin light chain in the Siberian sturgeon, Acipenser baeri</title><pii><json:string>S0145-305X(99)00049-X</json:string></pii><genre><json:string>research-article</json:string></genre><host><volume>23</volume><pii><json:string>S0145-305X(00)X0029-8</json:string></pii><pages><last>615</last><first>607</first></pages><issn><json:string>0145-305X</json:string></issn><issue>7–8</issue><genre><json:string>journal</json:string></genre><language><json:string>unknown</json:string></language><title>Developmental and Comparative Immunology</title><publicationDate>1999</publicationDate></host><categories><wos><json:string>science</json:string><json:string>zoology</json:string><json:string>immunology</json:string></wos><scienceMetrix><json:string>health sciences</json:string><json:string>clinical medicine</json:string><json:string>immunology</json:string></scienceMetrix></categories><publicationDate>1999</publicationDate><copyrightDate>1999</copyrightDate><doi><json:string>10.1016/S0145-305X(99)00049-X</json:string></doi><id>1A8D510C3AFB1B631F2A6D43945472E90570DA63</id><score>0.070117824</score><fulltext><json:item><extension>pdf</extension><original>true</original><mimetype>application/pdf</mimetype><uri>https://api.istex.fr/document/1A8D510C3AFB1B631F2A6D43945472E90570DA63/fulltext/pdf</uri></json:item><json:item><extension>zip</extension><original>false</original><mimetype>application/zip</mimetype><uri>https://api.istex.fr/document/1A8D510C3AFB1B631F2A6D43945472E90570DA63/fulltext/zip</uri></json:item><istex:fulltextTEI uri="https://api.istex.fr/document/1A8D510C3AFB1B631F2A6D43945472E90570DA63/fulltext/tei"><teiHeader><fileDesc><titleStmt><title level="a">Variability of the immunoglobulin light chain in the Siberian sturgeon, Acipenser baeri</title></titleStmt><publicationStmt><authority>ISTEX</authority><publisher>ELSEVIER</publisher><availability><p>©1999 Elsevier Science Ltd</p></availability><date>1999</date></publicationStmt><notesStmt><note type="content">Fig. 1: Plot of the Shannon entropy values (H) obtained from the 79 isolated IgVL cDNA clones of Acipenser baeri. The borders between the Shannon entropy plateaus [11] are shown in bold lines. The sequences have been deposited in the EMBL data base with acc. no. AJ387781–AJ387859. The specially indicated positions are numbered according to [15].</note><note type="content">Fig. 2: Southern blot of sturgeon erythrocyte DNA digested with EcoRI (E), BamHI (B), and HindIII (H), separately and in combinations. The filter was hybridized with a DNA probe specific for the second VL subfamily region and washed under high stringency conditions. The size markers are indicated to the left.</note><note type="content">Fig. 3: CDR length distribution in IgVL cDNA clones of the Siberian sturgeon. The borders of the CDR regions were set according to [15].</note><note type="content">Fig. 4: Physical map of the genomic clone SgLV2.5.1, EMBL acc no AJ245365. The region coding for the Leader peptide and the V segment are indicated by boxes and are presented as single letter aa sequence above the nt sequence. The exons are written in uppercase letters and the intronic sequences in lower case letters. Start codon is in bold, RSS in bold lower case letters, and potential regulatory elements are indicated as follows. Tata-box (TATAA), Octamer motif (ATTTGCAT), and E-boxes (CANNTG). One E-box and a partial octamer motif are overlapping around nt position 8700. Restriction sites are indicated with the initial letter, BamHI (b), SacI (s), PstI (p), EcoRI (e), and XbaI (x).</note><note type="content">Table 1: The mean entropies of the CDRs from different species, calculated by ANOVA in Statview with a significance limit of <0.05. The figures for Acipenser baeri are taken from this paper and the others from [9]</note></notesStmt><sourceDesc><biblStruct type="inbook"><analytic><title level="a">Variability of the immunoglobulin light chain in the Siberian sturgeon, Acipenser baeri</title><author xml:id="author-1"><persName><forename type="first">Mats L</forename><surname>Lundqvist</surname></persName><affiliation>Department of Cell and Molecular Biology, Immunology Programme, BMC, Uppsala University, PO Box 596, S-751 24 Uppsala, Sweden</affiliation></author><author xml:id="author-2"><persName><forename type="first">Lars</forename><surname>Pilström</surname></persName><email>Lars.Pilstrom@icm.uu.se</email><note type="correspondence"><p>Corresponding author. Tel.: +46-18-471-42-18; fax: +46-18-55-75-79</p></note><affiliation>Department of Cell and Molecular Biology, Immunology Programme, BMC, Uppsala University, PO Box 596, S-751 24 Uppsala, Sweden</affiliation></author></analytic><monogr><title level="j">Developmental and Comparative Immunology</title><title level="j" type="abbrev">DCI</title><idno type="pISSN">0145-305X</idno><idno type="PII">S0145-305X(00)X0029-8</idno><imprint><publisher>ELSEVIER</publisher><date type="published" when="1999"></date><biblScope unit="volume">23</biblScope><biblScope unit="issue">7–8</biblScope><biblScope unit="page" from="607">607</biblScope><biblScope unit="page" to="615">615</biblScope></imprint></monogr><idno type="istex">1A8D510C3AFB1B631F2A6D43945472E90570DA63</idno><idno type="DOI">10.1016/S0145-305X(99)00049-X</idno><idno type="PII">S0145-305X(99)00049-X</idno></biblStruct></sourceDesc></fileDesc><profileDesc><creation><date>1999</date></creation><langUsage><language ident="en">en</language></langUsage><abstract xml:lang="en"><p>All sturgeon VL segments isolated in this study belong to a single family, VLI, which can be divided into two subfamilies. Of the 79 cDNA clones isolated, 76 belong to the larger subfamily, VLIa, and only 3 clones constitute the smaller subfamily, VLIb. To evaluate variability, the Shannon entropy was estimated for each individual amino acid position, and to facilitate comparisons of variability between species the mean entropy of the CDR regions was calculated. In such a comparison, the sturgeon was found to have CDR1 and CDR3 variability approaching those found in mouse and clawed frog, but showed very low variability for CDR2. Amino acid position 50 does however display variability in the range of mouse and clawed frog. It is further confirmed that the sturgeon has numerous J segments, but that the junctional diversity does not contribute greatly to the diversity of the light chain. Comparisons of cDNA clones and a genomic VL segment indicate that the VL undergoes changes, particularly in the CDR regions, in a manner that can be explained by somatic hypermutation and/or gene conversion.</p></abstract><textClass><keywords scheme="keyword"><list><head>Keywords</head><item><term>Immunoglobulin</term></item><item><term>Light chain</term></item><item><term>VL gene</term></item><item><term>Diversity</term></item><item><term>Evolution</term></item><item><term>Siberian sturgeon</term></item><item><term>Acipenser baeri</term></item></list></keywords></textClass><textClass><keywords scheme="keyword"><list><head>Abbreviations</head><item><term>Ig, Immunoglobulin</term></item><item><term>LP, Leader peptide</term></item><item><term>V, Variable</term></item><item><term>J, Joining</term></item><item><term>CDR, Complementarity determining region</term></item><item><term>FR, Framework</term></item><item><term>RSS, Recombination signal sequence</term></item><item><term>aa, Amino acid</term></item><item><term>nt, Nucleotide(s)</term></item></list></keywords></textClass></profileDesc><revisionDesc><change when="1999">Published</change></revisionDesc></teiHeader></istex:fulltextTEI><json:item><extension>txt</extension><original>false</original><mimetype>text/plain</mimetype><uri>https://api.istex.fr/document/1A8D510C3AFB1B631F2A6D43945472E90570DA63/fulltext/txt</uri></json:item></fulltext><metadata><istex:metadataXml wicri:clean="Elsevier, elements deleted: ce:floats; body; tail"><istex:xmlDeclaration>version="1.0" encoding="utf-8"</istex:xmlDeclaration><istex:docType PUBLIC="-//ES//DTD journal article DTD version 4.5.2//EN//XML" URI="art452.dtd" name="istex:docType"><istex:entity SYSTEM="gr1" NDATA="IMAGE" name="gr1"></istex:entity><istex:entity SYSTEM="gr2" NDATA="IMAGE" name="gr2"></istex:entity><istex:entity SYSTEM="gr3" NDATA="IMAGE" name="gr3"></istex:entity><istex:entity SYSTEM="gr4" NDATA="IMAGE" name="gr4"></istex:entity></istex:docType><istex:document><converted-article version="4.5.2" docsubtype="fla"><item-info><jid>DCI</jid><aid>312</aid><ce:pii>S0145-305X(99)00049-X</ce:pii><ce:doi>10.1016/S0145-305X(99)00049-X</ce:doi><ce:copyright type="full-transfer" year="1999">Elsevier Science Ltd</ce:copyright></item-info><head><ce:title>Variability of the immunoglobulin light chain in the Siberian sturgeon, <ce:italic>Acipenser baeri</ce:italic></ce:title><ce:author-group><ce:author><ce:given-name>Mats L</ce:given-name><ce:surname>Lundqvist</ce:surname></ce:author><ce:author><ce:given-name>Lars</ce:given-name><ce:surname>Pilström</ce:surname><ce:cross-ref refid="CORR1">*</ce:cross-ref><ce:e-address>Lars.Pilstrom@icm.uu.se</ce:e-address></ce:author><ce:affiliation><ce:textfn>Department of Cell and Molecular Biology, Immunology Programme, BMC, Uppsala University, PO Box 596, S-751 24 Uppsala, Sweden</ce:textfn></ce:affiliation><ce:correspondence id="CORR1"><ce:label>*</ce:label><ce:text>Corresponding author. Tel.: +46-18-471-42-18; fax: +46-18-55-75-79</ce:text></ce:correspondence></ce:author-group><ce:date-received day="2" month="8" year="1999"></ce:date-received><ce:date-accepted day="19" month="8" year="1999"></ce:date-accepted><ce:abstract><ce:section-title>Abstract</ce:section-title><ce:abstract-sec><ce:simple-para>All sturgeon VL segments isolated in this study belong to a single family, VLI, which can be divided into two subfamilies. Of the 79 cDNA clones isolated, 76 belong to the larger subfamily, VLIa, and only 3 clones constitute the smaller subfamily, VLIb. To evaluate variability, the Shannon entropy was estimated for each individual amino acid position, and to facilitate comparisons of variability between species the mean entropy of the CDR regions was calculated. In such a comparison, the sturgeon was found to have CDR1 and CDR3 variability approaching those found in mouse and clawed frog, but showed very low variability for CDR2. Amino acid position 50 does however display variability in the range of mouse and clawed frog. It is further confirmed that the sturgeon has numerous J segments, but that the junctional diversity does not contribute greatly to the diversity of the light chain. Comparisons of cDNA clones and a genomic VL segment indicate that the VL undergoes changes, particularly in the CDR regions, in a manner that can be explained by somatic hypermutation and/or gene conversion.</ce:simple-para></ce:abstract-sec></ce:abstract><ce:keywords class="keyword"><ce:section-title>Keywords</ce:section-title><ce:keyword><ce:text>Immunoglobulin</ce:text></ce:keyword><ce:keyword><ce:text>Light chain</ce:text></ce:keyword><ce:keyword><ce:text>VL gene</ce:text></ce:keyword><ce:keyword><ce:text>Diversity</ce:text></ce:keyword><ce:keyword><ce:text>Evolution</ce:text></ce:keyword><ce:keyword><ce:text>Siberian sturgeon</ce:text></ce:keyword><ce:keyword><ce:text><ce:italic>Acipenser baeri</ce:italic></ce:text></ce:keyword></ce:keywords><ce:keywords class="abr"><ce:section-title>Abbreviations</ce:section-title><ce:keyword><ce:text>Ig, Immunoglobulin</ce:text></ce:keyword><ce:keyword><ce:text>LP, Leader peptide</ce:text></ce:keyword><ce:keyword><ce:text>V, Variable</ce:text></ce:keyword><ce:keyword><ce:text>J, Joining</ce:text></ce:keyword><ce:keyword><ce:text>CDR, Complementarity determining region</ce:text></ce:keyword><ce:keyword><ce:text>FR, Framework</ce:text></ce:keyword><ce:keyword><ce:text>RSS, Recombination signal sequence</ce:text></ce:keyword><ce:keyword><ce:text>aa, Amino acid</ce:text></ce:keyword><ce:keyword><ce:text>nt, Nucleotide(s)</ce:text></ce:keyword></ce:keywords></head></converted-article></istex:document></istex:metadataXml><mods version="3.6"><titleInfo><title>Variability of the immunoglobulin light chain in the Siberian sturgeon, Acipenser baeri</title></titleInfo><titleInfo type="alternative" contentType="CDATA"><title>Variability of the immunoglobulin light chain in the Siberian sturgeon,</title></titleInfo><name type="personal"><namePart type="given">Mats L</namePart><namePart type="family">Lundqvist</namePart><affiliation>Department of Cell and Molecular Biology, Immunology Programme, BMC, Uppsala University, PO Box 596, S-751 24 Uppsala, Sweden</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">Lars</namePart><namePart type="family">Pilström</namePart><affiliation>Department of Cell and Molecular Biology, Immunology Programme, BMC, Uppsala University, PO Box 596, S-751 24 Uppsala, Sweden</affiliation><affiliation>E-mail: Lars.Pilstrom@icm.uu.se</affiliation><description>Corresponding author. Tel.: +46-18-471-42-18; fax: +46-18-55-75-79</description><role><roleTerm type="text">author</roleTerm></role></name><typeOfResource>text</typeOfResource><genre type="research-article" displayLabel="Full-length article"></genre><originInfo><publisher>ELSEVIER</publisher><dateIssued encoding="w3cdtf">1999</dateIssued><copyrightDate encoding="w3cdtf">1999</copyrightDate></originInfo><language><languageTerm type="code" authority="iso639-2b">eng</languageTerm><languageTerm type="code" authority="rfc3066">en</languageTerm></language><physicalDescription><internetMediaType>text/html</internetMediaType></physicalDescription><abstract lang="en">All sturgeon VL segments isolated in this study belong to a single family, VLI, which can be divided into two subfamilies. Of the 79 cDNA clones isolated, 76 belong to the larger subfamily, VLIa, and only 3 clones constitute the smaller subfamily, VLIb. To evaluate variability, the Shannon entropy was estimated for each individual amino acid position, and to facilitate comparisons of variability between species the mean entropy of the CDR regions was calculated. In such a comparison, the sturgeon was found to have CDR1 and CDR3 variability approaching those found in mouse and clawed frog, but showed very low variability for CDR2. Amino acid position 50 does however display variability in the range of mouse and clawed frog. It is further confirmed that the sturgeon has numerous J segments, but that the junctional diversity does not contribute greatly to the diversity of the light chain. Comparisons of cDNA clones and a genomic VL segment indicate that the VL undergoes changes, particularly in the CDR regions, in a manner that can be explained by somatic hypermutation and/or gene conversion.</abstract><note type="content">Fig. 1: Plot of the Shannon entropy values (H) obtained from the 79 isolated IgVL cDNA clones of Acipenser baeri. The borders between the Shannon entropy plateaus [11] are shown in bold lines. The sequences have been deposited in the EMBL data base with acc. no. AJ387781–AJ387859. The specially indicated positions are numbered according to [15].</note><note type="content">Fig. 2: Southern blot of sturgeon erythrocyte DNA digested with EcoRI (E), BamHI (B), and HindIII (H), separately and in combinations. The filter was hybridized with a DNA probe specific for the second VL subfamily region and washed under high stringency conditions. The size markers are indicated to the left.</note><note type="content">Fig. 3: CDR length distribution in IgVL cDNA clones of the Siberian sturgeon. The borders of the CDR regions were set according to [15].</note><note type="content">Fig. 4: Physical map of the genomic clone SgLV2.5.1, EMBL acc no AJ245365. The region coding for the Leader peptide and the V segment are indicated by boxes and are presented as single letter aa sequence above the nt sequence. The exons are written in uppercase letters and the intronic sequences in lower case letters. Start codon is in bold, RSS in bold lower case letters, and potential regulatory elements are indicated as follows. Tata-box (TATAA), Octamer motif (ATTTGCAT), and E-boxes (CANNTG). One E-box and a partial octamer motif are overlapping around nt position 8700. Restriction sites are indicated with the initial letter, BamHI (b), SacI (s), PstI (p), EcoRI (e), and XbaI (x).</note><note type="content">Table 1: The mean entropies of the CDRs from different species, calculated by ANOVA in Statview with a significance limit of <0.05. The figures for Acipenser baeri are taken from this paper and the others from [9]</note><subject><genre>Keywords</genre><topic>Immunoglobulin</topic><topic>Light chain</topic><topic>VL gene</topic><topic>Diversity</topic><topic>Evolution</topic><topic>Siberian sturgeon</topic><topic>Acipenser baeri</topic></subject><subject><genre>Abbreviations</genre><topic>Ig, Immunoglobulin</topic><topic>LP, Leader peptide</topic><topic>V, Variable</topic><topic>J, Joining</topic><topic>CDR, Complementarity determining region</topic><topic>FR, Framework</topic><topic>RSS, Recombination signal sequence</topic><topic>aa, Amino acid</topic><topic>nt, Nucleotide(s)</topic></subject><relatedItem type="host"><titleInfo><title>Developmental and Comparative Immunology</title></titleInfo><titleInfo type="abbreviated"><title>DCI</title></titleInfo><genre type="journal">journal</genre><originInfo><dateIssued encoding="w3cdtf">199910</dateIssued></originInfo><identifier type="ISSN">0145-305X</identifier><identifier type="PII">S0145-305X(00)X0029-8</identifier><part><date>199910</date><detail type="volume"><number>23</number><caption>vol.</caption></detail><detail type="issue"><number>7–8</number><caption>no.</caption></detail><extent unit="issue pages"><start>535</start><end>708</end></extent><extent unit="pages"><start>607</start><end>615</end></extent></part></relatedItem><identifier type="istex">1A8D510C3AFB1B631F2A6D43945472E90570DA63</identifier><identifier type="DOI">10.1016/S0145-305X(99)00049-X</identifier><identifier type="PII">S0145-305X(99)00049-X</identifier><accessCondition type="use and reproduction" contentType="copyright">©1999 Elsevier Science Ltd</accessCondition><recordInfo><recordContentSource>ELSEVIER</recordContentSource><recordOrigin>Elsevier Science Ltd, ©1999</recordOrigin></recordInfo></mods></metadata><serie></serie></istex></record>Pour manipuler ce document sous Unix (Dilib)
EXPLOR_STEP=$WICRI_ROOT/Wicri/Eau/explor/EsturgeonV1/Data/Istex/Corpus
HfdSelect -h $EXPLOR_STEP/biblio.hfd -nk 000B35 | SxmlIndent | more
Ou
HfdSelect -h $EXPLOR_AREA/Data/Istex/Corpus/biblio.hfd -nk 000B35 | SxmlIndent | more
Pour mettre un lien sur cette page dans le réseau Wicri
{{Explor lien
|wiki= Wicri/Eau
|area= EsturgeonV1
|flux= Istex
|étape= Corpus
|type= RBID
|clé= ISTEX:1A8D510C3AFB1B631F2A6D43945472E90570DA63
|texte= Variability of the immunoglobulin light chain in the Siberian sturgeon, Acipenser baeri
}}
| This area was generated with Dilib version V0.6.27. |  |