Enhanced 5-methylcytosine detection in single-molecule, real-time sequencing via Tet1 oxidation.
Identifieur interne : 001314 ( Main/Exploration ); précédent : 001313; suivant : 001315Enhanced 5-methylcytosine detection in single-molecule, real-time sequencing via Tet1 oxidation.
Auteurs : Tyson A. Clark [États-Unis] ; Xingyu Lu ; Khai Luong ; Qing Dai ; Matthew Boitano ; Stephen W. Turner ; Chuan He ; Jonas KorlachSource :
- BMC biology [ 1741-7007 ] ; 2013.
Descripteurs français
- KwdFr :
- 5-Méthyl-cytosine (métabolisme), Analyse de séquence d'ADN (MeSH), Cinétique (MeSH), DNA modification methylases (métabolisme), Escherichia coli (enzymologie), Génome bactérien (MeSH), Mixed function oxygenases (MeSH), Oxydoréduction (MeSH), Protéines de liaison à l'ADN (métabolisme), Protéines proto-oncogènes (métabolisme), Spécificité du substrat (MeSH).
- MESH :
English descriptors
- KwdEn :
- 5-Methylcytosine (metabolism), DNA Modification Methylases (metabolism), DNA-Binding Proteins (metabolism), Escherichia coli (enzymology), Genome, Bacterial (MeSH), Kinetics (MeSH), Mixed Function Oxygenases (MeSH), Oxidation-Reduction (MeSH), Proto-Oncogene Proteins (metabolism), Sequence Analysis, DNA (MeSH), Substrate Specificity (MeSH).
- MESH :
- chemical , metabolism : 5-Methylcytosine, DNA Modification Methylases, DNA-Binding Proteins, Proto-Oncogene Proteins.
- enzymology : Escherichia coli.
- Genome, Bacterial, Kinetics, Mixed Function Oxygenases, Oxidation-Reduction, Sequence Analysis, DNA, Substrate Specificity.
Abstract
BACKGROUND
DNA methylation serves as an important epigenetic mark in both eukaryotic and prokaryotic organisms. In eukaryotes, the most common epigenetic mark is 5-methylcytosine, whereas prokaryotes can have 6-methyladenine, 4-methylcytosine, or 5-methylcytosine. Single-molecule, real-time sequencing is capable of directly detecting all three types of modified bases. However, the kinetic signature of 5-methylcytosine is subtle, which presents a challenge for detection. We investigated whether conversion of 5-methylcytosine to 5-carboxylcytosine using the enzyme Tet1 would enhance the kinetic signature, thereby improving detection.
RESULTS
We characterized the kinetic signatures of various cytosine modifications, demonstrating that 5-carboxylcytosine has a larger impact on the local polymerase rate than 5-methylcytosine. Using Tet1-mediated conversion, we show improved detection of 5-methylcytosine using in vitro methylated templates and apply the method to the characterization of 5-methylcytosine sites in the genomes of Escherichia coli MG1655 and Bacillus halodurans C-125.
CONCLUSIONS
We have developed a method for the enhancement of directly detecting 5-methylcytosine during single-molecule, real-time sequencing. Using Tet1 to convert 5-methylcytosine to 5-carboxylcytosine improves the detection rate of this important epigenetic marker, thereby complementing the set of readily detectable microbial base modifications, and enhancing the ability to interrogate eukaryotic epigenetic markers.
DOI: 10.1186/1741-7007-11-4
PubMed: 23339471
PubMed Central: PMC3598637
Affiliations:
Links toward previous steps (curation, corpus...)
Le document en format XML
<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">Enhanced 5-methylcytosine detection in single-molecule, real-time sequencing via Tet1 oxidation.</title><author><name sortKey="Clark, Tyson A" sort="Clark, Tyson A" uniqKey="Clark T" first="Tyson A" last="Clark">Tyson A. Clark</name><affiliation wicri:level="2"><nlm:affiliation>Pacific Biosciences, 1380 Willow Road, Menlo Park, CA 94025, USA.</nlm:affiliation><country xml:lang="fr">États-Unis</country><wicri:regionArea>Pacific Biosciences, 1380 Willow Road, Menlo Park, CA 94025</wicri:regionArea><placeName><region type="state">Californie</region></placeName></affiliation></author><author><name sortKey="Lu, Xingyu" sort="Lu, Xingyu" uniqKey="Lu X" first="Xingyu" last="Lu">Xingyu Lu</name></author><author><name sortKey="Luong, Khai" sort="Luong, Khai" uniqKey="Luong K" first="Khai" last="Luong">Khai Luong</name></author><author><name sortKey="Dai, Qing" sort="Dai, Qing" uniqKey="Dai Q" first="Qing" last="Dai">Qing Dai</name></author><author><name sortKey="Boitano, Matthew" sort="Boitano, Matthew" uniqKey="Boitano M" first="Matthew" last="Boitano">Matthew Boitano</name></author><author><name sortKey="Turner, Stephen W" sort="Turner, Stephen W" uniqKey="Turner S" first="Stephen W" last="Turner">Stephen W. Turner</name></author><author><name sortKey="He, Chuan" sort="He, Chuan" uniqKey="He C" first="Chuan" last="He">Chuan He</name></author><author><name sortKey="Korlach, Jonas" sort="Korlach, Jonas" uniqKey="Korlach J" first="Jonas" last="Korlach">Jonas Korlach</name></author></titleStmt><publicationStmt><idno type="wicri:source">PubMed</idno><date when="2013">2013</date><idno type="RBID">pubmed:23339471</idno><idno type="pmid">23339471</idno><idno type="doi">10.1186/1741-7007-11-4</idno><idno type="pmc">PMC3598637</idno><idno type="wicri:Area/Main/Corpus">001329</idno><idno type="wicri:explorRef" wicri:stream="Main" wicri:step="Corpus" wicri:corpus="PubMed">001329</idno><idno type="wicri:Area/Main/Curation">001329</idno><idno type="wicri:explorRef" wicri:stream="Main" wicri:step="Curation">001329</idno><idno type="wicri:Area/Main/Exploration">001329</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en">Enhanced 5-methylcytosine detection in single-molecule, real-time sequencing via Tet1 oxidation.</title><author><name sortKey="Clark, Tyson A" sort="Clark, Tyson A" uniqKey="Clark T" first="Tyson A" last="Clark">Tyson A. Clark</name><affiliation wicri:level="2"><nlm:affiliation>Pacific Biosciences, 1380 Willow Road, Menlo Park, CA 94025, USA.</nlm:affiliation><country xml:lang="fr">États-Unis</country><wicri:regionArea>Pacific Biosciences, 1380 Willow Road, Menlo Park, CA 94025</wicri:regionArea><placeName><region type="state">Californie</region></placeName></affiliation></author><author><name sortKey="Lu, Xingyu" sort="Lu, Xingyu" uniqKey="Lu X" first="Xingyu" last="Lu">Xingyu Lu</name></author><author><name sortKey="Luong, Khai" sort="Luong, Khai" uniqKey="Luong K" first="Khai" last="Luong">Khai Luong</name></author><author><name sortKey="Dai, Qing" sort="Dai, Qing" uniqKey="Dai Q" first="Qing" last="Dai">Qing Dai</name></author><author><name sortKey="Boitano, Matthew" sort="Boitano, Matthew" uniqKey="Boitano M" first="Matthew" last="Boitano">Matthew Boitano</name></author><author><name sortKey="Turner, Stephen W" sort="Turner, Stephen W" uniqKey="Turner S" first="Stephen W" last="Turner">Stephen W. Turner</name></author><author><name sortKey="He, Chuan" sort="He, Chuan" uniqKey="He C" first="Chuan" last="He">Chuan He</name></author><author><name sortKey="Korlach, Jonas" sort="Korlach, Jonas" uniqKey="Korlach J" first="Jonas" last="Korlach">Jonas Korlach</name></author></analytic><series><title level="j">BMC biology</title><idno type="eISSN">1741-7007</idno><imprint><date when="2013" type="published">2013</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>5-Methylcytosine (metabolism)</term><term>DNA Modification Methylases (metabolism)</term><term>DNA-Binding Proteins (metabolism)</term><term>Escherichia coli (enzymology)</term><term>Genome, Bacterial (MeSH)</term><term>Kinetics (MeSH)</term><term>Mixed Function Oxygenases (MeSH)</term><term>Oxidation-Reduction (MeSH)</term><term>Proto-Oncogene Proteins (metabolism)</term><term>Sequence Analysis, DNA (MeSH)</term><term>Substrate Specificity (MeSH)</term></keywords><keywords scheme="KwdFr" xml:lang="fr"><term>5-Méthyl-cytosine (métabolisme)</term><term>Analyse de séquence d'ADN (MeSH)</term><term>Cinétique (MeSH)</term><term>DNA modification methylases (métabolisme)</term><term>Escherichia coli (enzymologie)</term><term>Génome bactérien (MeSH)</term><term>Mixed function oxygenases (MeSH)</term><term>Oxydoréduction (MeSH)</term><term>Protéines de liaison à l'ADN (métabolisme)</term><term>Protéines proto-oncogènes (métabolisme)</term><term>Spécificité du substrat (MeSH)</term></keywords><keywords scheme="MESH" type="chemical" qualifier="metabolism" xml:lang="en"><term>5-Methylcytosine</term><term>DNA Modification Methylases</term><term>DNA-Binding Proteins</term><term>Proto-Oncogene Proteins</term></keywords><keywords scheme="MESH" qualifier="enzymologie" xml:lang="fr"><term>Escherichia coli</term></keywords><keywords scheme="MESH" qualifier="enzymology" xml:lang="en"><term>Escherichia coli</term></keywords><keywords scheme="MESH" qualifier="métabolisme" xml:lang="fr"><term>5-Méthyl-cytosine</term><term>DNA modification methylases</term><term>Protéines de liaison à l'ADN</term><term>Protéines proto-oncogènes</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Genome, Bacterial</term><term>Kinetics</term><term>Mixed Function Oxygenases</term><term>Oxidation-Reduction</term><term>Sequence Analysis, DNA</term><term>Substrate Specificity</term></keywords><keywords scheme="MESH" xml:lang="fr"><term>Analyse de séquence d'ADN</term><term>Cinétique</term><term>Génome bactérien</term><term>Mixed function oxygenases</term><term>Oxydoréduction</term><term>Spécificité du substrat</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en"><p><b>BACKGROUND</b></p><p>DNA methylation serves as an important epigenetic mark in both eukaryotic and prokaryotic organisms. In eukaryotes, the most common epigenetic mark is 5-methylcytosine, whereas prokaryotes can have 6-methyladenine, 4-methylcytosine, or 5-methylcytosine. Single-molecule, real-time sequencing is capable of directly detecting all three types of modified bases. However, the kinetic signature of 5-methylcytosine is subtle, which presents a challenge for detection. We investigated whether conversion of 5-methylcytosine to 5-carboxylcytosine using the enzyme Tet1 would enhance the kinetic signature, thereby improving detection.</p></div><div type="abstract" xml:lang="en"><p><b>RESULTS</b></p><p>We characterized the kinetic signatures of various cytosine modifications, demonstrating that 5-carboxylcytosine has a larger impact on the local polymerase rate than 5-methylcytosine. Using Tet1-mediated conversion, we show improved detection of 5-methylcytosine using in vitro methylated templates and apply the method to the characterization of 5-methylcytosine sites in the genomes of Escherichia coli MG1655 and Bacillus halodurans C-125.</p></div><div type="abstract" xml:lang="en"><p><b>CONCLUSIONS</b></p><p>We have developed a method for the enhancement of directly detecting 5-methylcytosine during single-molecule, real-time sequencing. Using Tet1 to convert 5-methylcytosine to 5-carboxylcytosine improves the detection rate of this important epigenetic marker, thereby complementing the set of readily detectable microbial base modifications, and enhancing the ability to interrogate eukaryotic epigenetic markers.</p></div></front></TEI><pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">23339471</PMID><DateCompleted><Year>2013</Year><Month>06</Month><Day>11</Day></DateCompleted><DateRevised><Year>2018</Year><Month>11</Month><Day>13</Day></DateRevised><Article PubModel="Electronic"><Journal><ISSN IssnType="Electronic">1741-7007</ISSN><JournalIssue CitedMedium="Internet"><Volume>11</Volume><PubDate><Year>2013</Year><Month>Jan</Month><Day>22</Day></PubDate></JournalIssue><Title>BMC biology</Title><ISOAbbreviation>BMC Biol</ISOAbbreviation></Journal><ArticleTitle>Enhanced 5-methylcytosine detection in single-molecule, real-time sequencing via Tet1 oxidation.</ArticleTitle><Pagination><MedlinePgn>4</MedlinePgn></Pagination><ELocationID EIdType="doi" ValidYN="Y">10.1186/1741-7007-11-4</ELocationID><Abstract><AbstractText Label="BACKGROUND" NlmCategory="BACKGROUND">DNA methylation serves as an important epigenetic mark in both eukaryotic and prokaryotic organisms. In eukaryotes, the most common epigenetic mark is 5-methylcytosine, whereas prokaryotes can have 6-methyladenine, 4-methylcytosine, or 5-methylcytosine. Single-molecule, real-time sequencing is capable of directly detecting all three types of modified bases. However, the kinetic signature of 5-methylcytosine is subtle, which presents a challenge for detection. We investigated whether conversion of 5-methylcytosine to 5-carboxylcytosine using the enzyme Tet1 would enhance the kinetic signature, thereby improving detection.</AbstractText><AbstractText Label="RESULTS" NlmCategory="RESULTS">We characterized the kinetic signatures of various cytosine modifications, demonstrating that 5-carboxylcytosine has a larger impact on the local polymerase rate than 5-methylcytosine. Using Tet1-mediated conversion, we show improved detection of 5-methylcytosine using in vitro methylated templates and apply the method to the characterization of 5-methylcytosine sites in the genomes of Escherichia coli MG1655 and Bacillus halodurans C-125.</AbstractText><AbstractText Label="CONCLUSIONS" NlmCategory="CONCLUSIONS">We have developed a method for the enhancement of directly detecting 5-methylcytosine during single-molecule, real-time sequencing. Using Tet1 to convert 5-methylcytosine to 5-carboxylcytosine improves the detection rate of this important epigenetic marker, thereby complementing the set of readily detectable microbial base modifications, and enhancing the ability to interrogate eukaryotic epigenetic markers.</AbstractText></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Clark</LastName><ForeName>Tyson A</ForeName><Initials>TA</Initials><AffiliationInfo><Affiliation>Pacific Biosciences, 1380 Willow Road, Menlo Park, CA 94025, USA.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Lu</LastName><ForeName>Xingyu</ForeName><Initials>X</Initials></Author><Author ValidYN="Y"><LastName>Luong</LastName><ForeName>Khai</ForeName><Initials>K</Initials></Author><Author ValidYN="Y"><LastName>Dai</LastName><ForeName>Qing</ForeName><Initials>Q</Initials></Author><Author ValidYN="Y"><LastName>Boitano</LastName><ForeName>Matthew</ForeName><Initials>M</Initials></Author><Author ValidYN="Y"><LastName>Turner</LastName><ForeName>Stephen W</ForeName><Initials>SW</Initials></Author><Author ValidYN="Y"><LastName>He</LastName><ForeName>Chuan</ForeName><Initials>C</Initials></Author><Author ValidYN="Y"><LastName>Korlach</LastName><ForeName>Jonas</ForeName><Initials>J</Initials></Author></AuthorList><Language>eng</Language><GrantList CompleteYN="Y"><Grant><GrantID>K01 HG006699</GrantID><Acronym>HG</Acronym><Agency>NHGRI NIH HHS</Agency><Country>United States</Country></Grant></GrantList><PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType></PublicationTypeList><ArticleDate DateType="Electronic"><Year>2013</Year><Month>01</Month><Day>22</Day></ArticleDate></Article><MedlineJournalInfo><Country>England</Country><MedlineTA>BMC Biol</MedlineTA><NlmUniqueID>101190720</NlmUniqueID><ISSNLinking>1741-7007</ISSNLinking></MedlineJournalInfo><ChemicalList><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D004268">DNA-Binding Proteins</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D011518">Proto-Oncogene Proteins</NameOfSubstance></Chemical><Chemical><RegistryNumber>6R795CQT4H</RegistryNumber><NameOfSubstance UI="D044503">5-Methylcytosine</NameOfSubstance></Chemical><Chemical><RegistryNumber>EC 1.-</RegistryNumber><NameOfSubstance UI="D006899">Mixed Function Oxygenases</NameOfSubstance></Chemical><Chemical><RegistryNumber>EC 1.-</RegistryNumber><NameOfSubstance UI="C462539">TET1 protein, human</NameOfSubstance></Chemical><Chemical><RegistryNumber>EC 2.1.1.-</RegistryNumber><NameOfSubstance UI="D015254">DNA Modification Methylases</NameOfSubstance></Chemical></ChemicalList><CitationSubset>IM</CitationSubset><MeshHeadingList><MeshHeading><DescriptorName UI="D044503" MajorTopicYN="N">5-Methylcytosine</DescriptorName><QualifierName UI="Q000378" MajorTopicYN="Y">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D015254" MajorTopicYN="N">DNA Modification Methylases</DescriptorName><QualifierName UI="Q000378" MajorTopicYN="N">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D004268" MajorTopicYN="N">DNA-Binding Proteins</DescriptorName><QualifierName UI="Q000378" MajorTopicYN="Y">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D004926" MajorTopicYN="N">Escherichia coli</DescriptorName><QualifierName UI="Q000201" MajorTopicYN="N">enzymology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D016680" MajorTopicYN="N">Genome, Bacterial</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D007700" MajorTopicYN="N">Kinetics</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D006899" MajorTopicYN="N">Mixed Function Oxygenases</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D010084" MajorTopicYN="N">Oxidation-Reduction</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D011518" MajorTopicYN="N">Proto-Oncogene Proteins</DescriptorName><QualifierName UI="Q000378" MajorTopicYN="Y">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D017422" MajorTopicYN="Y">Sequence Analysis, DNA</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D013379" MajorTopicYN="N">Substrate Specificity</DescriptorName></MeshHeading></MeshHeadingList></MedlineCitation><PubmedData><History><PubMedPubDate PubStatus="received"><Year>2012</Year><Month>07</Month><Day>24</Day></PubMedPubDate><PubMedPubDate PubStatus="accepted"><Year>2013</Year><Month>01</Month><Day>22</Day></PubMedPubDate><PubMedPubDate PubStatus="entrez"><Year>2013</Year><Month>1</Month><Day>24</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="pubmed"><Year>2013</Year><Month>1</Month><Day>24</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="medline"><Year>2013</Year><Month>6</Month><Day>12</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate></History><PublicationStatus>epublish</PublicationStatus><ArticleIdList><ArticleId IdType="pubmed">23339471</ArticleId><ArticleId IdType="pii">1741-7007-11-4</ArticleId><ArticleId IdType="doi">10.1186/1741-7007-11-4</ArticleId><ArticleId IdType="pmc">PMC3598637</ArticleId></ArticleIdList><ReferenceList><Reference><Citation>Nat Biotechnol. 2012 Jul;30(7):693-700</Citation><ArticleIdList><ArticleId IdType="pubmed">22750884</ArticleId></ArticleIdList></Reference><Reference><Citation>Science. 2011 Sep 2;333(6047):1303-7</Citation><ArticleIdList><ArticleId IdType="pubmed">21817016</ArticleId></ArticleIdList></Reference><Reference><Citation>Nat Biotechnol. 2012 Jul;30(7):701-7</Citation><ArticleIdList><ArticleId IdType="pubmed">22750883</ArticleId></ArticleIdList></Reference><Reference><Citation>Nat Chem Biol. 2012 Apr;8(4):328-30</Citation><ArticleIdList><ArticleId IdType="pubmed">22327402</ArticleId></ArticleIdList></Reference><Reference><Citation>Genes Dev. 2011 Dec 1;25(23):2436-52</Citation><ArticleIdList><ArticleId IdType="pubmed">22156206</ArticleId></ArticleIdList></Reference><Reference><Citation>Angew Chem Int Ed Engl. 2011 Jul 25;50(31):7008-12</Citation><ArticleIdList><ArticleId IdType="pubmed">21721093</ArticleId></ArticleIdList></Reference><Reference><Citation>Genome Res. 2009 Sep;19(9):1639-45</Citation><ArticleIdList><ArticleId IdType="pubmed">19541911</ArticleId></ArticleIdList></Reference><Reference><Citation>Cell. 2012 Jun 8;149(6):1368-80</Citation><ArticleIdList><ArticleId IdType="pubmed">22608086</ArticleId></ArticleIdList></Reference><Reference><Citation>Bioinformatics. 2011 Jun 15;27(12):1696-7</Citation><ArticleIdList><ArticleId IdType="pubmed">21486936</ArticleId></ArticleIdList></Reference><Reference><Citation>Nucleic Acids Res. 2012 Feb;40(4):e29</Citation><ArticleIdList><ArticleId IdType="pubmed">22156058</ArticleId></ArticleIdList></Reference><Reference><Citation>Mol Cell. 2004 Nov 19;16(4):609-18</Citation><ArticleIdList><ArticleId IdType="pubmed">15546620</ArticleId></ArticleIdList></Reference><Reference><Citation>Nucleic Acids Res. 2012 Dec;40(22):11450-62</Citation><ArticleIdList><ArticleId IdType="pubmed">23034806</ArticleId></ArticleIdList></Reference><Reference><Citation>Genes Dev. 2002 Jan 1;16(1):6-21</Citation><ArticleIdList><ArticleId IdType="pubmed">11782440</ArticleId></ArticleIdList></Reference><Reference><Citation>Science. 2009 Jan 2;323(5910):133-8</Citation><ArticleIdList><ArticleId IdType="pubmed">19023044</ArticleId></ArticleIdList></Reference><Reference><Citation>Infect Immun. 2001 Dec;69(12):7197-204</Citation><ArticleIdList><ArticleId IdType="pubmed">11705888</ArticleId></ArticleIdList></Reference><Reference><Citation>Science. 2011 Sep 2;333(6047):1300-3</Citation><ArticleIdList><ArticleId IdType="pubmed">21778364</ArticleId></ArticleIdList></Reference><Reference><Citation>Science. 2012 May 18;336(6083):934-7</Citation><ArticleIdList><ArticleId IdType="pubmed">22539555</ArticleId></ArticleIdList></Reference><Reference><Citation>Annu Rev Genet. 1991;25:585-627</Citation><ArticleIdList><ArticleId IdType="pubmed">1812816</ArticleId></ArticleIdList></Reference><Reference><Citation>Angew Chem Int Ed Engl. 2011 Jul 11;50(29):6460-8</Citation><ArticleIdList><ArticleId IdType="pubmed">21688365</ArticleId></ArticleIdList></Reference><Reference><Citation>PLoS One. 2010;5(1):e8888</Citation><ArticleIdList><ArticleId IdType="pubmed">20126651</ArticleId></ArticleIdList></Reference><Reference><Citation>Nucleic Acids Res. 2010 Jan;38(Database issue):D234-6</Citation><ArticleIdList><ArticleId IdType="pubmed">19846593</ArticleId></ArticleIdList></Reference><Reference><Citation>Nat Methods. 2010 Jun;7(6):461-5</Citation><ArticleIdList><ArticleId IdType="pubmed">20453866</ArticleId></ArticleIdList></Reference><Reference><Citation>Science. 2009 May 15;324(5929):929-30</Citation><ArticleIdList><ArticleId IdType="pubmed">19372393</ArticleId></ArticleIdList></Reference><Reference><Citation>Nat Methods. 2012 Jan;9(1):75-7</Citation><ArticleIdList><ArticleId IdType="pubmed">22101853</ArticleId></ArticleIdList></Reference><Reference><Citation>Nat Biotechnol. 2012 Dec;30(12):1232-9</Citation><ArticleIdList><ArticleId IdType="pubmed">23138224</ArticleId></ArticleIdList></Reference><Reference><Citation>Nucleic Acids Res. 2010 Aug;38(15):e159</Citation><ArticleIdList><ArticleId IdType="pubmed">20571086</ArticleId></ArticleIdList></Reference><Reference><Citation>Genome Integr. 2011 Dec 20;2:10</Citation><ArticleIdList><ArticleId IdType="pubmed">22185597</ArticleId></ArticleIdList></Reference><Reference><Citation>Genome Res. 2013 Jan;23(1):129-41</Citation><ArticleIdList><ArticleId IdType="pubmed">23093720</ArticleId></ArticleIdList></Reference><Reference><Citation>Methods Enzymol. 2010;472:431-55</Citation><ArticleIdList><ArticleId IdType="pubmed">20580975</ArticleId></ArticleIdList></Reference><Reference><Citation>FEMS Microbiol Rev. 2009 May;33(3):488-503</Citation><ArticleIdList><ArticleId IdType="pubmed">19175412</ArticleId></ArticleIdList></Reference><Reference><Citation>Science. 2009 May 15;324(5929):930-5</Citation><ArticleIdList><ArticleId IdType="pubmed">19372391</ArticleId></ArticleIdList></Reference></ReferenceList></PubmedData></pubmed><affiliations><list><country><li>États-Unis</li></country><region><li>Californie</li></region></list><tree><noCountry><name sortKey="Boitano, Matthew" sort="Boitano, Matthew" uniqKey="Boitano M" first="Matthew" last="Boitano">Matthew Boitano</name><name sortKey="Dai, Qing" sort="Dai, Qing" uniqKey="Dai Q" first="Qing" last="Dai">Qing Dai</name><name sortKey="He, Chuan" sort="He, Chuan" uniqKey="He C" first="Chuan" last="He">Chuan He</name><name sortKey="Korlach, Jonas" sort="Korlach, Jonas" uniqKey="Korlach J" first="Jonas" last="Korlach">Jonas Korlach</name><name sortKey="Lu, Xingyu" sort="Lu, Xingyu" uniqKey="Lu X" first="Xingyu" last="Lu">Xingyu Lu</name><name sortKey="Luong, Khai" sort="Luong, Khai" uniqKey="Luong K" first="Khai" last="Luong">Khai Luong</name><name sortKey="Turner, Stephen W" sort="Turner, Stephen W" uniqKey="Turner S" first="Stephen W" last="Turner">Stephen W. Turner</name></noCountry><country name="États-Unis"><region name="Californie"><name sortKey="Clark, Tyson A" sort="Clark, Tyson A" uniqKey="Clark T" first="Tyson A" last="Clark">Tyson A. Clark</name></region></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
EXPLOR_STEP=$WICRI_ROOT/Bois/explor/WillowV1/Data/Main/Exploration
HfdSelect -h $EXPLOR_STEP/biblio.hfd -nk 001314 | SxmlIndent | more
Ou
HfdSelect -h $EXPLOR_AREA/Data/Main/Exploration/biblio.hfd -nk 001314 | SxmlIndent | more
Pour mettre un lien sur cette page dans le réseau Wicri
{{Explor lien
|wiki= Bois
|area= WillowV1
|flux= Main
|étape= Exploration
|type= RBID
|clé= pubmed:23339471
|texte= Enhanced 5-methylcytosine detection in single-molecule, real-time sequencing via Tet1 oxidation.
}}
Pour générer des pages wiki
HfdIndexSelect -h $EXPLOR_AREA/Data/Main/Exploration/RBID.i -Sk "pubmed:23339471" \
| HfdSelect -Kh $EXPLOR_AREA/Data/Main/Exploration/biblio.hfd \
| NlmPubMed2Wicri -a WillowV1
| This area was generated with Dilib version V0.6.37. |  |