Test of aerobic TCE degradation by willows (Salix viminalis) and willows inoculated with TCE-cometabolizing strains of Burkholderia cepacia.
Identifieur interne : 000923 ( Main/Corpus ); précédent : 000922; suivant : 000924Test of aerobic TCE degradation by willows (Salix viminalis) and willows inoculated with TCE-cometabolizing strains of Burkholderia cepacia.
Auteurs : Lauge Peter Westergaard Clausen ; Mette Martina Broholm ; Ulrich Gosewinkel ; Stefan TrappSource :
- Environmental science and pollution research international [ 1614-7499 ] ; 2017.
English descriptors
- KwdEn :
- Biodegradation, Environmental (MeSH), Burkholderia cepacia (growth & development), Hydroponics (MeSH), Salix (growth & development), Salix (metabolism), Salix (microbiology), Soil Pollutants (analysis), Soil Pollutants (metabolism), Soil Pollutants (toxicity), Trees (growth & development), Trees (metabolism), Trees (microbiology), Trichloroethylene (analysis), Trichloroethylene (metabolism), Trichloroethylene (toxicity).
- MESH :
- chemical , analysis : Soil Pollutants, Trichloroethylene.
- growth & development : Burkholderia cepacia, Salix, Trees.
- metabolism : Salix, Soil Pollutants, Trees, Trichloroethylene.
- microbiology : Salix, Trees.
- chemical , toxicity : Soil Pollutants, Trichloroethylene.
- Biodegradation, Environmental, Hydroponics.
Abstract
Trichloroethylene (TCE) is a widespread soil and groundwater pollutant and clean-up is often problematic and expensive. Phytoremediation may be a cost-effective solution at some sites. This study investigates TCE degradation by willows (S. viminalis) and willows inoculated with three strains of B. cepacia (301C, PR1-31 and VM1330-pTOM), using chloride formation as an indicator of dehalogenation. Willows were grown in non-sterile, hydroponic conditions for 3 weeks in chloride-free nutrient solution spiked with TCE. TCE was added weekly due to rapid loss by volatilization. Chloride and TCE in solution were measured every 2-3 days and chloride and metabolite concentrations in plants were measured at test termination. Based on transpiration, no tree toxicity of TCE exposure was observed. However, trees grown in chloride-free solution showed severely inhibited transpiration. No or very little chloride was formed during the test, and levels of chloride in TCE-exposed trees were not elevated. Chloride concentrations in chloride containing TCE-free nutrient solution doubled within 23 days, indicating active exclusion of chloride by root cell membranes. Only traces of TCE-metabolites were detected in plant tissue. We conclude that TCE is not, or to a limited extent (less than 3%), aerobically degraded by the willow trees. The three strains of B. cepacia did not enhance TCE mineralization. Future successful application of rhizo- and phytodegradation of TCE requires measures to be taken to improve the degradation rates.
DOI: 10.1007/s11356-017-9420-8
PubMed: 28639018
Links to Exploration step
pubmed:28639018Le document en format XML
<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">Test of aerobic TCE degradation by willows (Salix viminalis) and willows inoculated with TCE-cometabolizing strains of Burkholderia cepacia.</title><author><name sortKey="Clausen, Lauge Peter Westergaard" sort="Clausen, Lauge Peter Westergaard" uniqKey="Clausen L" first="Lauge Peter Westergaard" last="Clausen">Lauge Peter Westergaard Clausen</name><affiliation><nlm:affiliation>Department of Environmental Engineering, Technical University of Denmark, Bygningstorvet bygning 115, 2800, Kgs Lyngby, Denmark. lpwc@env.dtu.dk.</nlm:affiliation></affiliation></author><author><name sortKey="Broholm, Mette Martina" sort="Broholm, Mette Martina" uniqKey="Broholm M" first="Mette Martina" last="Broholm">Mette Martina Broholm</name><affiliation><nlm:affiliation>Department of Environmental Engineering, Technical University of Denmark, Bygningstorvet bygning 115, 2800, Kgs Lyngby, Denmark.</nlm:affiliation></affiliation></author><author><name sortKey="Gosewinkel, Ulrich" sort="Gosewinkel, Ulrich" uniqKey="Gosewinkel U" first="Ulrich" last="Gosewinkel">Ulrich Gosewinkel</name><affiliation><nlm:affiliation>Department of Environmental Science, Aarhus University, Frederiksborgvej 399, 4000, Roskilde, Denmark.</nlm:affiliation></affiliation></author><author><name sortKey="Trapp, Stefan" sort="Trapp, Stefan" uniqKey="Trapp S" first="Stefan" last="Trapp">Stefan Trapp</name><affiliation><nlm:affiliation>Department of Environmental Engineering, Technical University of Denmark, Bygningstorvet bygning 115, 2800, Kgs Lyngby, Denmark. sttr@env.dtu.dk.</nlm:affiliation></affiliation></author></titleStmt><publicationStmt><idno type="wicri:source">PubMed</idno><date when="2017">2017</date><idno type="RBID">pubmed:28639018</idno><idno type="pmid">28639018</idno><idno type="doi">10.1007/s11356-017-9420-8</idno><idno type="wicri:Area/Main/Corpus">000923</idno><idno 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Denmark, Bygningstorvet bygning 115, 2800, Kgs Lyngby, Denmark.</nlm:affiliation></affiliation></author><author><name sortKey="Gosewinkel, Ulrich" sort="Gosewinkel, Ulrich" uniqKey="Gosewinkel U" first="Ulrich" last="Gosewinkel">Ulrich Gosewinkel</name><affiliation><nlm:affiliation>Department of Environmental Science, Aarhus University, Frederiksborgvej 399, 4000, Roskilde, Denmark.</nlm:affiliation></affiliation></author><author><name sortKey="Trapp, Stefan" sort="Trapp, Stefan" uniqKey="Trapp S" first="Stefan" last="Trapp">Stefan Trapp</name><affiliation><nlm:affiliation>Department of Environmental Engineering, Technical University of Denmark, Bygningstorvet bygning 115, 2800, Kgs Lyngby, Denmark. sttr@env.dtu.dk.</nlm:affiliation></affiliation></author></analytic><series><title level="j">Environmental science and pollution research international</title><idno type="eISSN">1614-7499</idno><imprint><date when="2017" type="published">2017</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Biodegradation, Environmental (MeSH)</term><term>Burkholderia cepacia (growth & development)</term><term>Hydroponics (MeSH)</term><term>Salix (growth & development)</term><term>Salix (metabolism)</term><term>Salix (microbiology)</term><term>Soil Pollutants (analysis)</term><term>Soil Pollutants (metabolism)</term><term>Soil Pollutants (toxicity)</term><term>Trees (growth & development)</term><term>Trees (metabolism)</term><term>Trees (microbiology)</term><term>Trichloroethylene (analysis)</term><term>Trichloroethylene (metabolism)</term><term>Trichloroethylene (toxicity)</term></keywords><keywords scheme="MESH" type="chemical" qualifier="analysis" xml:lang="en"><term>Soil Pollutants</term><term>Trichloroethylene</term></keywords><keywords scheme="MESH" qualifier="growth & development" xml:lang="en"><term>Burkholderia cepacia</term><term>Salix</term><term>Trees</term></keywords><keywords scheme="MESH" qualifier="metabolism" xml:lang="en"><term>Salix</term><term>Soil Pollutants</term><term>Trees</term><term>Trichloroethylene</term></keywords><keywords scheme="MESH" qualifier="microbiology" xml:lang="en"><term>Salix</term><term>Trees</term></keywords><keywords scheme="MESH" type="chemical" qualifier="toxicity" xml:lang="en"><term>Soil Pollutants</term><term>Trichloroethylene</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Biodegradation, Environmental</term><term>Hydroponics</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Trichloroethylene (TCE) is a widespread soil and groundwater pollutant and clean-up is often problematic and expensive. Phytoremediation may be a cost-effective solution at some sites. This study investigates TCE degradation by willows (S. viminalis) and willows inoculated with three strains of B. cepacia (301C, PR1-31 and VM1330-pTOM), using chloride formation as an indicator of dehalogenation. Willows were grown in non-sterile, hydroponic conditions for 3 weeks in chloride-free nutrient solution spiked with TCE. TCE was added weekly due to rapid loss by volatilization. Chloride and TCE in solution were measured every 2-3 days and chloride and metabolite concentrations in plants were measured at test termination. Based on transpiration, no tree toxicity of TCE exposure was observed. However, trees grown in chloride-free solution showed severely inhibited transpiration. No or very little chloride was formed during the test, and levels of chloride in TCE-exposed trees were not elevated. Chloride concentrations in chloride containing TCE-free nutrient solution doubled within 23 days, indicating active exclusion of chloride by root cell membranes. Only traces of TCE-metabolites were detected in plant tissue. We conclude that TCE is not, or to a limited extent (less than 3%), aerobically degraded by the willow trees. The three strains of B. cepacia did not enhance TCE mineralization. Future successful application of rhizo- and phytodegradation of TCE requires measures to be taken to improve the degradation rates.</div></front></TEI><pubmed><MedlineCitation Status="MEDLINE" IndexingMethod="Curated" Owner="NLM"><PMID Version="1">28639018</PMID><DateCompleted><Year>2018</Year><Month>10</Month><Day>30</Day></DateCompleted><DateRevised><Year>2018</Year><Month>11</Month><Day>13</Day></DateRevised><Article PubModel="Print-Electronic"><Journal><ISSN IssnType="Electronic">1614-7499</ISSN><JournalIssue CitedMedium="Internet"><Volume>24</Volume><Issue>22</Issue><PubDate><Year>2017</Year><Month>Aug</Month></PubDate></JournalIssue><Title>Environmental science and pollution research international</Title><ISOAbbreviation>Environ Sci Pollut Res Int</ISOAbbreviation></Journal><ArticleTitle>Test of aerobic TCE degradation by willows (Salix viminalis) and willows inoculated with TCE-cometabolizing strains of Burkholderia cepacia.</ArticleTitle><Pagination><MedlinePgn>18320-18331</MedlinePgn></Pagination><ELocationID EIdType="doi" ValidYN="Y">10.1007/s11356-017-9420-8</ELocationID><Abstract><AbstractText>Trichloroethylene (TCE) is a widespread soil and groundwater pollutant and clean-up is often problematic and expensive. Phytoremediation may be a cost-effective solution at some sites. This study investigates TCE degradation by willows (S. viminalis) and willows inoculated with three strains of B. cepacia (301C, PR1-31 and VM1330-pTOM), using chloride formation as an indicator of dehalogenation. Willows were grown in non-sterile, hydroponic conditions for 3 weeks in chloride-free nutrient solution spiked with TCE. TCE was added weekly due to rapid loss by volatilization. Chloride and TCE in solution were measured every 2-3 days and chloride and metabolite concentrations in plants were measured at test termination. Based on transpiration, no tree toxicity of TCE exposure was observed. However, trees grown in chloride-free solution showed severely inhibited transpiration. No or very little chloride was formed during the test, and levels of chloride in TCE-exposed trees were not elevated. Chloride concentrations in chloride containing TCE-free nutrient solution doubled within 23 days, indicating active exclusion of chloride by root cell membranes. Only traces of TCE-metabolites were detected in plant tissue. We conclude that TCE is not, or to a limited extent (less than 3%), aerobically degraded by the willow trees. The three strains of B. cepacia did not enhance TCE mineralization. Future successful application of rhizo- and phytodegradation of TCE requires measures to be taken to improve the degradation rates.</AbstractText></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Clausen</LastName><ForeName>Lauge Peter Westergaard</ForeName><Initials>LPW</Initials><AffiliationInfo><Affiliation>Department of Environmental Engineering, Technical University of Denmark, Bygningstorvet bygning 115, 2800, Kgs Lyngby, Denmark. lpwc@env.dtu.dk.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Broholm</LastName><ForeName>Mette Martina</ForeName><Initials>MM</Initials><AffiliationInfo><Affiliation>Department of Environmental Engineering, Technical University of Denmark, Bygningstorvet bygning 115, 2800, Kgs Lyngby, Denmark.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Gosewinkel</LastName><ForeName>Ulrich</ForeName><Initials>U</Initials><AffiliationInfo><Affiliation>Department of Environmental Science, Aarhus University, Frederiksborgvej 399, 4000, Roskilde, Denmark.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Trapp</LastName><ForeName>Stefan</ForeName><Initials>S</Initials><AffiliationInfo><Affiliation>Department of Environmental Engineering, Technical University of Denmark, Bygningstorvet bygning 115, 2800, Kgs Lyngby, Denmark. sttr@env.dtu.dk.</Affiliation></AffiliationInfo></Author></AuthorList><Language>eng</Language><PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType></PublicationTypeList><ArticleDate DateType="Electronic"><Year>2017</Year><Month>06</Month><Day>21</Day></ArticleDate></Article><MedlineJournalInfo><Country>Germany</Country><MedlineTA>Environ Sci Pollut Res Int</MedlineTA><NlmUniqueID>9441769</NlmUniqueID><ISSNLinking>0944-1344</ISSNLinking></MedlineJournalInfo><ChemicalList><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D012989">Soil Pollutants</NameOfSubstance></Chemical><Chemical><RegistryNumber>290YE8AR51</RegistryNumber><NameOfSubstance UI="D014241">Trichloroethylene</NameOfSubstance></Chemical></ChemicalList><CitationSubset>IM</CitationSubset><MeshHeadingList><MeshHeading><DescriptorName UI="D001673" MajorTopicYN="N">Biodegradation, Environmental</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D016956" MajorTopicYN="N">Burkholderia cepacia</DescriptorName><QualifierName UI="Q000254" MajorTopicYN="Y">growth & development</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D018527" MajorTopicYN="N">Hydroponics</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D032108" MajorTopicYN="N">Salix</DescriptorName><QualifierName UI="Q000254" MajorTopicYN="N">growth & development</QualifierName><QualifierName UI="Q000378" MajorTopicYN="Y">metabolism</QualifierName><QualifierName UI="Q000382" MajorTopicYN="N">microbiology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D012989" MajorTopicYN="N">Soil Pollutants</DescriptorName><QualifierName UI="Q000032" MajorTopicYN="Y">analysis</QualifierName><QualifierName UI="Q000378" MajorTopicYN="N">metabolism</QualifierName><QualifierName UI="Q000633" MajorTopicYN="N">toxicity</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D014197" MajorTopicYN="N">Trees</DescriptorName><QualifierName UI="Q000254" MajorTopicYN="N">growth & development</QualifierName><QualifierName UI="Q000378" MajorTopicYN="Y">metabolism</QualifierName><QualifierName UI="Q000382" MajorTopicYN="N">microbiology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D014241" MajorTopicYN="N">Trichloroethylene</DescriptorName><QualifierName UI="Q000032" MajorTopicYN="Y">analysis</QualifierName><QualifierName UI="Q000378" MajorTopicYN="N">metabolism</QualifierName><QualifierName UI="Q000633" MajorTopicYN="N">toxicity</QualifierName></MeshHeading></MeshHeadingList><KeywordList Owner="NOTNLM"><Keyword MajorTopicYN="N">B. cepacia</Keyword><Keyword MajorTopicYN="N">Chloride</Keyword><Keyword MajorTopicYN="N">Dehalogenation</Keyword><Keyword MajorTopicYN="N">Phytoremediation</Keyword><Keyword MajorTopicYN="N">TCE degradation</Keyword><Keyword MajorTopicYN="N">Trichloroethylene</Keyword><Keyword MajorTopicYN="N">Willow tree toxicity test</Keyword></KeywordList></MedlineCitation><PubmedData><History><PubMedPubDate PubStatus="received"><Year>2017</Year><Month>01</Month><Day>16</Day></PubMedPubDate><PubMedPubDate PubStatus="accepted"><Year>2017</Year><Month>05</Month><Day>30</Day></PubMedPubDate><PubMedPubDate PubStatus="pubmed"><Year>2017</Year><Month>6</Month><Day>24</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="medline"><Year>2018</Year><Month>10</Month><Day>31</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate 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