Investigations of microbial degradation of polycyclic aromatic hydrocarbons based on 13C-labeled phenanthrene in a soil co-contaminated with trace elements using a plant assisted approach.
Identifieur interne : 000810 ( Main/Corpus ); précédent : 000809; suivant : 000811Investigations of microbial degradation of polycyclic aromatic hydrocarbons based on 13C-labeled phenanthrene in a soil co-contaminated with trace elements using a plant assisted approach.
Auteurs : Anna Wawra ; Wolfgang Friesl-Hanl ; Anna J Ger ; Markus Puschenreiter ; Gerhard Soja ; Thomas Reichenauer ; Andrea WatzingerSource :
- Environmental science and pollution research international [ 1614-7499 ] ; 2018.
English descriptors
- KwdEn :
- Austria (MeSH), Biodegradation, Environmental (MeSH), Carbon Isotopes (analysis), Metals, Heavy (analysis), Models, Theoretical (MeSH), Phenanthrenes (analysis), Polycyclic Aromatic Hydrocarbons (analysis), Robinia (growth & development), Salix (growth & development), Soil (MeSH), Soil Microbiology (MeSH), Soil Pollutants (analysis), Trace Elements (analysis).
- MESH :
- chemical , analysis : Carbon Isotopes, Metals, Heavy, Phenanthrenes, Polycyclic Aromatic Hydrocarbons, Soil Pollutants, Trace Elements.
- geographic : Austria, Soil.
- growth & development : Robinia, Salix.
- Biodegradation, Environmental, Models, Theoretical, Soil Microbiology.
Abstract
Co-contaminations of soils with organic and inorganic pollutants are a frequent environmental problem. Due to their toxicity and recalcitrance, the heterogeneous pollutants may persist in soil. The hypothesis of this study was that degradation of polycyclic aromatic hydrocarbons (PAHs) is enhanced if heavy metals in soil are immobilized and their bioavailability reduced. For metal immobilization and enhanced biodegradation, distinct mineral and organic soil amendments (iron oxides, gravel sludge, biochar) were deployed in an incubation batch experiment. The second part of the experiment consisted of a greenhouse pot experiment applying fast-growing and pollution-tolerant woody plants (willow and black locust). Soil amendments initially immobilized NH4NO3-extractable zinc, cadmium, and lead; after 100 days of incubation, soil amendments showed reductions only for cadmium and a tendency to enhance arsenic mobility. In order to monitor the remediation success, a 13C-phenanthrene (PHE) label was applied. 13C-phospholipid fatty acid analysis (13C-PLFA) further enabled the identification of PHE-degrading soil microorganisms. Both experiments exhibited a similar PLFA profile. Gram-negative bacteria (esp. cy17:0, 16:1ω7 + 6, 18:1ω7c) were the most significant microbial group taking up 13C-PHE. Plants effectively increased the label uptake by gram-positive bacteria and increased the biomass of the fungal biomarker, although their contribution to the degradation process was minor. Plants tended to prolong PAH dissipation in soil; at the end of the experiment, however, all treatments showed equally low total PAH concentrations in soil. While black locust plants tended not to take up potentially toxic trace elements, willows accumulated them in their leaves. The results of this study show that the chosen treatments did not enhance the remediation of the experimental soil.
DOI: 10.1007/s11356-017-0941-y
PubMed: 29249024
Links to Exploration step
pubmed:29249024Le document en format XML
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Wolfgang.Friesl-Hanl@ait.ac.at.</nlm:affiliation></affiliation></author><author><name sortKey="J Ger, Anna" sort="J Ger, Anna" uniqKey="J Ger A" first="Anna" last="J Ger">Anna J Ger</name><affiliation><nlm:affiliation>Environmental Resources & Technologies, AIT Austrian Institute of Technology GmbH, Konrad-Lorenz-Straße 24, 3430, Tulln, Austria.</nlm:affiliation></affiliation></author><author><name sortKey="Puschenreiter, Markus" sort="Puschenreiter, Markus" uniqKey="Puschenreiter M" first="Markus" last="Puschenreiter">Markus Puschenreiter</name><affiliation><nlm:affiliation>Institute of Soil Research, University of Natural Resources and Life Sciences (BOKU), Konrad-Lorenz-Straße 24, 3430, Tulln, Austria.</nlm:affiliation></affiliation></author><author><name sortKey="Soja, Gerhard" sort="Soja, Gerhard" uniqKey="Soja G" first="Gerhard" last="Soja">Gerhard Soja</name><affiliation><nlm:affiliation>Environmental Resources & Technologies, AIT Austrian Institute of Technology GmbH, Konrad-Lorenz-Straße 24, 3430, Tulln, Austria.</nlm:affiliation></affiliation></author><author><name sortKey="Reichenauer, Thomas" sort="Reichenauer, Thomas" uniqKey="Reichenauer T" first="Thomas" last="Reichenauer">Thomas Reichenauer</name><affiliation><nlm:affiliation>Environmental Resources & Technologies, AIT Austrian Institute of Technology GmbH, Konrad-Lorenz-Straße 24, 3430, Tulln, Austria.</nlm:affiliation></affiliation></author><author><name sortKey="Watzinger, Andrea" sort="Watzinger, Andrea" uniqKey="Watzinger A" first="Andrea" last="Watzinger">Andrea Watzinger</name><affiliation><nlm:affiliation>Environmental Resources & Technologies, AIT Austrian Institute of Technology GmbH, Konrad-Lorenz-Straße 24, 3430, Tulln, Austria.</nlm:affiliation></affiliation></author></titleStmt><publicationStmt><idno type="wicri:source">PubMed</idno><date when="2018">2018</date><idno type="RBID">pubmed:29249024</idno><idno type="pmid">29249024</idno><idno type="doi">10.1007/s11356-017-0941-y</idno><idno type="wicri:Area/Main/Corpus">000810</idno><idno type="wicri:explorRef" wicri:stream="Main" wicri:step="Corpus" wicri:corpus="PubMed">000810</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en">Investigations of microbial degradation of polycyclic aromatic hydrocarbons based on <sup>13</sup>C-labeled phenanthrene in a soil co-contaminated with trace elements using a plant assisted approach.</title><author><name sortKey="Wawra, Anna" sort="Wawra, Anna" uniqKey="Wawra A" first="Anna" last="Wawra">Anna Wawra</name><affiliation><nlm:affiliation>Environmental Resources & Technologies, AIT Austrian Institute of Technology GmbH, Konrad-Lorenz-Straße 24, 3430, Tulln, Austria.</nlm:affiliation></affiliation><affiliation><nlm:affiliation>Institute of Soil Research, University of Natural Resources and Life Sciences (BOKU), Konrad-Lorenz-Straße 24, 3430, Tulln, Austria.</nlm:affiliation></affiliation></author><author><name sortKey="Friesl Hanl, Wolfgang" sort="Friesl Hanl, Wolfgang" uniqKey="Friesl Hanl W" first="Wolfgang" last="Friesl-Hanl">Wolfgang Friesl-Hanl</name><affiliation><nlm:affiliation>Environmental Resources & Technologies, AIT Austrian Institute of Technology GmbH, Konrad-Lorenz-Straße 24, 3430, Tulln, Austria. Wolfgang.Friesl-Hanl@ait.ac.at.</nlm:affiliation></affiliation></author><author><name sortKey="J Ger, Anna" sort="J Ger, Anna" uniqKey="J Ger A" first="Anna" last="J Ger">Anna J Ger</name><affiliation><nlm:affiliation>Environmental Resources & Technologies, AIT Austrian Institute of Technology GmbH, Konrad-Lorenz-Straße 24, 3430, Tulln, Austria.</nlm:affiliation></affiliation></author><author><name sortKey="Puschenreiter, Markus" sort="Puschenreiter, Markus" uniqKey="Puschenreiter M" first="Markus" last="Puschenreiter">Markus Puschenreiter</name><affiliation><nlm:affiliation>Institute of Soil Research, University of Natural Resources and Life Sciences (BOKU), Konrad-Lorenz-Straße 24, 3430, Tulln, Austria.</nlm:affiliation></affiliation></author><author><name sortKey="Soja, Gerhard" sort="Soja, Gerhard" uniqKey="Soja G" first="Gerhard" last="Soja">Gerhard Soja</name><affiliation><nlm:affiliation>Environmental Resources & Technologies, AIT Austrian Institute of Technology GmbH, Konrad-Lorenz-Straße 24, 3430, Tulln, Austria.</nlm:affiliation></affiliation></author><author><name sortKey="Reichenauer, Thomas" sort="Reichenauer, Thomas" uniqKey="Reichenauer T" first="Thomas" last="Reichenauer">Thomas Reichenauer</name><affiliation><nlm:affiliation>Environmental Resources & Technologies, AIT Austrian Institute of Technology GmbH, Konrad-Lorenz-Straße 24, 3430, Tulln, Austria.</nlm:affiliation></affiliation></author><author><name sortKey="Watzinger, Andrea" sort="Watzinger, Andrea" uniqKey="Watzinger A" first="Andrea" last="Watzinger">Andrea Watzinger</name><affiliation><nlm:affiliation>Environmental Resources & Technologies, AIT Austrian Institute of Technology GmbH, Konrad-Lorenz-Straße 24, 3430, Tulln, Austria.</nlm:affiliation></affiliation></author></analytic><series><title level="j">Environmental science and pollution research international</title><idno type="eISSN">1614-7499</idno><imprint><date when="2018" type="published">2018</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Austria (MeSH)</term><term>Biodegradation, Environmental (MeSH)</term><term>Carbon Isotopes (analysis)</term><term>Metals, Heavy (analysis)</term><term>Models, Theoretical (MeSH)</term><term>Phenanthrenes (analysis)</term><term>Polycyclic Aromatic Hydrocarbons (analysis)</term><term>Robinia (growth & development)</term><term>Salix (growth & development)</term><term>Soil (MeSH)</term><term>Soil Microbiology (MeSH)</term><term>Soil Pollutants (analysis)</term><term>Trace Elements (analysis)</term></keywords><keywords scheme="MESH" type="chemical" qualifier="analysis" xml:lang="en"><term>Carbon Isotopes</term><term>Metals, Heavy</term><term>Phenanthrenes</term><term>Polycyclic Aromatic Hydrocarbons</term><term>Soil Pollutants</term><term>Trace Elements</term></keywords><keywords scheme="MESH" type="geographic" xml:lang="en"><term>Austria</term><term>Soil</term></keywords><keywords scheme="MESH" qualifier="growth & development" xml:lang="en"><term>Robinia</term><term>Salix</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Biodegradation, Environmental</term><term>Models, Theoretical</term><term>Soil Microbiology</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Co-contaminations of soils with organic and inorganic pollutants are a frequent environmental problem. Due to their toxicity and recalcitrance, the heterogeneous pollutants may persist in soil. The hypothesis of this study was that degradation of polycyclic aromatic hydrocarbons (PAHs) is enhanced if heavy metals in soil are immobilized and their bioavailability reduced. For metal immobilization and enhanced biodegradation, distinct mineral and organic soil amendments (iron oxides, gravel sludge, biochar) were deployed in an incubation batch experiment. The second part of the experiment consisted of a greenhouse pot experiment applying fast-growing and pollution-tolerant woody plants (willow and black locust). Soil amendments initially immobilized NH<sub>4</sub>NO<sub>3</sub>-extractable zinc, cadmium, and lead; after 100 days of incubation, soil amendments showed reductions only for cadmium and a tendency to enhance arsenic mobility. In order to monitor the remediation success, a <sup>13</sup>C-phenanthrene (PHE) label was applied. <sup>13</sup>C-phospholipid fatty acid analysis (<sup>13</sup>C-PLFA) further enabled the identification of PHE-degrading soil microorganisms. Both experiments exhibited a similar PLFA profile. Gram-negative bacteria (esp. cy17:0, 16:1ω7 + 6, 18:1ω7c) were the most significant microbial group taking up <sup>13</sup>C-PHE. Plants effectively increased the label uptake by gram-positive bacteria and increased the biomass of the fungal biomarker, although their contribution to the degradation process was minor. Plants tended to prolong PAH dissipation in soil; at the end of the experiment, however, all treatments showed equally low total PAH concentrations in soil. While black locust plants tended not to take up potentially toxic trace elements, willows accumulated them in their leaves. The results of this study show that the chosen treatments did not enhance the remediation of the experimental soil.</div></front></TEI><pubmed><MedlineCitation Status="MEDLINE" IndexingMethod="Curated" Owner="NLM"><PMID Version="1">29249024</PMID><DateCompleted><Year>2018</Year><Month>11</Month><Day>26</Day></DateCompleted><DateRevised><Year>2019</Year><Month>01</Month><Day>01</Day></DateRevised><Article PubModel="Print-Electronic"><Journal><ISSN IssnType="Electronic">1614-7499</ISSN><JournalIssue CitedMedium="Internet"><Volume>25</Volume><Issue>7</Issue><PubDate><Year>2018</Year><Month>Mar</Month></PubDate></JournalIssue><Title>Environmental science and pollution research international</Title><ISOAbbreviation>Environ Sci Pollut Res Int</ISOAbbreviation></Journal><ArticleTitle>Investigations of microbial degradation of polycyclic aromatic hydrocarbons based on <sup>13</sup>C-labeled phenanthrene in a soil co-contaminated with trace elements using a plant assisted approach.</ArticleTitle><Pagination><MedlinePgn>6364-6377</MedlinePgn></Pagination><ELocationID EIdType="doi" ValidYN="Y">10.1007/s11356-017-0941-y</ELocationID><Abstract><AbstractText>Co-contaminations of soils with organic and inorganic pollutants are a frequent environmental problem. Due to their toxicity and recalcitrance, the heterogeneous pollutants may persist in soil. The hypothesis of this study was that degradation of polycyclic aromatic hydrocarbons (PAHs) is enhanced if heavy metals in soil are immobilized and their bioavailability reduced. For metal immobilization and enhanced biodegradation, distinct mineral and organic soil amendments (iron oxides, gravel sludge, biochar) were deployed in an incubation batch experiment. The second part of the experiment consisted of a greenhouse pot experiment applying fast-growing and pollution-tolerant woody plants (willow and black locust). Soil amendments initially immobilized NH<sub>4</sub>NO<sub>3</sub>-extractable zinc, cadmium, and lead; after 100 days of incubation, soil amendments showed reductions only for cadmium and a tendency to enhance arsenic mobility. In order to monitor the remediation success, a <sup>13</sup>C-phenanthrene (PHE) label was applied. <sup>13</sup>C-phospholipid fatty acid analysis (<sup>13</sup>C-PLFA) further enabled the identification of PHE-degrading soil microorganisms. Both experiments exhibited a similar PLFA profile. Gram-negative bacteria (esp. cy17:0, 16:1ω7 + 6, 18:1ω7c) were the most significant microbial group taking up <sup>13</sup>C-PHE. Plants effectively increased the label uptake by gram-positive bacteria and increased the biomass of the fungal biomarker, although their contribution to the degradation process was minor. Plants tended to prolong PAH dissipation in soil; at the end of the experiment, however, all treatments showed equally low total PAH concentrations in soil. While black locust plants tended not to take up potentially toxic trace elements, willows accumulated them in their leaves. The results of this study show that the chosen treatments did not enhance the remediation of the experimental soil.</AbstractText></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Wawra</LastName><ForeName>Anna</ForeName><Initials>A</Initials><AffiliationInfo><Affiliation>Environmental Resources & Technologies, AIT Austrian Institute of Technology GmbH, Konrad-Lorenz-Straße 24, 3430, Tulln, Austria.</Affiliation></AffiliationInfo><AffiliationInfo><Affiliation>Institute of Soil Research, University of Natural Resources and Life Sciences (BOKU), Konrad-Lorenz-Straße 24, 3430, Tulln, Austria.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Friesl-Hanl</LastName><ForeName>Wolfgang</ForeName><Initials>W</Initials><AffiliationInfo><Affiliation>Environmental Resources & Technologies, AIT Austrian Institute of Technology GmbH, Konrad-Lorenz-Straße 24, 3430, Tulln, Austria. Wolfgang.Friesl-Hanl@ait.ac.at.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Jäger</LastName><ForeName>Anna</ForeName><Initials>A</Initials><AffiliationInfo><Affiliation>Environmental Resources & Technologies, AIT Austrian Institute of Technology GmbH, Konrad-Lorenz-Straße 24, 3430, Tulln, Austria.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Puschenreiter</LastName><ForeName>Markus</ForeName><Initials>M</Initials><AffiliationInfo><Affiliation>Institute of Soil Research, University of Natural Resources and Life Sciences (BOKU), Konrad-Lorenz-Straße 24, 3430, Tulln, Austria.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Soja</LastName><ForeName>Gerhard</ForeName><Initials>G</Initials><AffiliationInfo><Affiliation>Environmental Resources & Technologies, AIT Austrian Institute of Technology GmbH, Konrad-Lorenz-Straße 24, 3430, Tulln, Austria.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Reichenauer</LastName><ForeName>Thomas</ForeName><Initials>T</Initials><AffiliationInfo><Affiliation>Environmental Resources & Technologies, AIT Austrian Institute of Technology GmbH, Konrad-Lorenz-Straße 24, 3430, Tulln, Austria.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Watzinger</LastName><ForeName>Andrea</ForeName><Initials>A</Initials><AffiliationInfo><Affiliation>Environmental Resources & Technologies, AIT Austrian Institute of Technology GmbH, Konrad-Lorenz-Straße 24, 3430, Tulln, Austria.</Affiliation></AffiliationInfo></Author></AuthorList><Language>eng</Language><GrantList CompleteYN="Y"><Grant><GrantID>WST3-T-81/029-2011, WST3-T-81/034-2014, WST3-T-81/036-2016</GrantID><Agency>EFRD european fund for regional development and County of lower Austria</Agency><Country></Country></Grant></GrantList><PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType></PublicationTypeList><ArticleDate DateType="Electronic"><Year>2017</Year><Month>12</Month><Day>16</Day></ArticleDate></Article><MedlineJournalInfo><Country>Germany</Country><MedlineTA>Environ Sci Pollut Res Int</MedlineTA><NlmUniqueID>9441769</NlmUniqueID><ISSNLinking>0944-1344</ISSNLinking></MedlineJournalInfo><ChemicalList><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D002247">Carbon Isotopes</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D019216">Metals, Heavy</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D010616">Phenanthrenes</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D011084">Polycyclic Aromatic Hydrocarbons</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D012987">Soil</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D012989">Soil Pollutants</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D014131">Trace Elements</NameOfSubstance></Chemical><Chemical><RegistryNumber>448J8E5BST</RegistryNumber><NameOfSubstance UI="C031181">phenanthrene</NameOfSubstance></Chemical><Chemical><RegistryNumber>FDJ0A8596D</RegistryNumber><NameOfSubstance UI="C000615229">Carbon-13</NameOfSubstance></Chemical></ChemicalList><CitationSubset>IM</CitationSubset><MeshHeadingList><MeshHeading><DescriptorName UI="D001317" MajorTopicYN="N" Type="Geographic">Austria</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D001673" MajorTopicYN="N">Biodegradation, Environmental</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D002247" MajorTopicYN="N">Carbon Isotopes</DescriptorName><QualifierName UI="Q000032" MajorTopicYN="N">analysis</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D019216" MajorTopicYN="N">Metals, Heavy</DescriptorName><QualifierName UI="Q000032" MajorTopicYN="N">analysis</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D008962" MajorTopicYN="N">Models, Theoretical</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D010616" MajorTopicYN="N">Phenanthrenes</DescriptorName><QualifierName UI="Q000032" MajorTopicYN="N">analysis</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D011084" MajorTopicYN="N">Polycyclic Aromatic Hydrocarbons</DescriptorName><QualifierName UI="Q000032" MajorTopicYN="Y">analysis</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D031301" MajorTopicYN="N">Robinia</DescriptorName><QualifierName UI="Q000254" MajorTopicYN="Y">growth & development</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D032108" MajorTopicYN="N">Salix</DescriptorName><QualifierName UI="Q000254" MajorTopicYN="Y">growth & development</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D012987" MajorTopicYN="N">Soil</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D012988" MajorTopicYN="Y">Soil Microbiology</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D012989" MajorTopicYN="N">Soil Pollutants</DescriptorName><QualifierName UI="Q000032" MajorTopicYN="Y">analysis</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D014131" MajorTopicYN="N">Trace Elements</DescriptorName><QualifierName UI="Q000032" MajorTopicYN="Y">analysis</QualifierName></MeshHeading></MeshHeadingList><KeywordList Owner="NOTNLM"><Keyword MajorTopicYN="N">13C-Phospholipid fatty acid analysis</Keyword><Keyword MajorTopicYN="N">Phytoremediation</Keyword><Keyword MajorTopicYN="N">Polycyclic aromatic hydrocarbons</Keyword><Keyword MajorTopicYN="N">Soil pollution</Keyword><Keyword MajorTopicYN="N">Stable isotopic label</Keyword><Keyword MajorTopicYN="N">Trace metals</Keyword></KeywordList></MedlineCitation><PubmedData><History><PubMedPubDate PubStatus="received"><Year>2017</Year><Month>06</Month><Day>27</Day></PubMedPubDate><PubMedPubDate PubStatus="accepted"><Year>2017</Year><Month>12</Month><Day>04</Day></PubMedPubDate><PubMedPubDate PubStatus="pubmed"><Year>2017</Year><Month>12</Month><Day>19</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="medline"><Year>2018</Year><Month>11</Month><Day>27</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="entrez"><Year>2017</Year><Month>12</Month><Day>18</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate></History><PublicationStatus>ppublish</PublicationStatus><ArticleIdList><ArticleId IdType="pubmed">29249024</ArticleId><ArticleId IdType="doi">10.1007/s11356-017-0941-y</ArticleId><ArticleId IdType="pii">10.1007/s11356-017-0941-y</ArticleId></ArticleIdList></PubmedData></pubmed></record>Pour manipuler ce document sous Unix (Dilib)
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